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fitc conjugated rabbit anti mouse igg antibody  (Bio-Rad)


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    Bio-Rad fitc conjugated rabbit anti mouse igg antibody
    IF of NF-κB activity induced by CypA in the THP-1 cell. ( a ) The u and d THP-1 cells were precubated with control (a, b, i, j) and AP-9 (c, d, k, l), then stimulated with CypA (200 ng/mL, b, d, j, l), CypA/CSA (f, n), LPS (1 µg/mL, g, o) or LPS/CSA (h, p) respectively for 1h. The p50 protein of NF-κB activity was analysed by IF with <t>FITC-conjugated</t> anti-p50 antibody (green fluorescence) for the uTHP-1 and Cy3-conjugated anti-p50 antibody (red fluorescence) for the dTHP-1, respectively. CypA-induced nuclear translocation of NF-κB 2 h after treatment (b and h). Nuclear translocation of NF-κB induced by CypA was blockaded by AP-9 (d and l) or CSA (f and n) respectively. ( b ) Merged images of Cy3 and DAPI in the dTHP-1 were obtained using computer software. After CypA stimulating THP-1, the red color for NF-κB overlayed with the blue color for nucleus in cell nucleus under immunofluorescence microscopy, which showed plenty of p50 transducted into nucleus. The scale bars indicate 25 μm ( a ) and 10 μm ( b ). The data shown are representative of similar results from three independent experiments.
    Fitc Conjugated Rabbit Anti Mouse Igg Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/curve+fitting+function+in+sigmaplot+software/pmc07107241-67-19-24?v=Bio-Rad
    Average 94 stars, based on 806 article reviews
    fitc conjugated rabbit anti mouse igg antibody - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis"

    Article Title: Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis

    Journal: Rheumatology (Oxford, England)

    doi: 10.1093/rheumatology/ken225

    IF of NF-κB activity induced by CypA in the THP-1 cell. ( a ) The u and d THP-1 cells were precubated with control (a, b, i, j) and AP-9 (c, d, k, l), then stimulated with CypA (200 ng/mL, b, d, j, l), CypA/CSA (f, n), LPS (1 µg/mL, g, o) or LPS/CSA (h, p) respectively for 1h. The p50 protein of NF-κB activity was analysed by IF with FITC-conjugated anti-p50 antibody (green fluorescence) for the uTHP-1 and Cy3-conjugated anti-p50 antibody (red fluorescence) for the dTHP-1, respectively. CypA-induced nuclear translocation of NF-κB 2 h after treatment (b and h). Nuclear translocation of NF-κB induced by CypA was blockaded by AP-9 (d and l) or CSA (f and n) respectively. ( b ) Merged images of Cy3 and DAPI in the dTHP-1 were obtained using computer software. After CypA stimulating THP-1, the red color for NF-κB overlayed with the blue color for nucleus in cell nucleus under immunofluorescence microscopy, which showed plenty of p50 transducted into nucleus. The scale bars indicate 25 μm ( a ) and 10 μm ( b ). The data shown are representative of similar results from three independent experiments.
    Figure Legend Snippet: IF of NF-κB activity induced by CypA in the THP-1 cell. ( a ) The u and d THP-1 cells were precubated with control (a, b, i, j) and AP-9 (c, d, k, l), then stimulated with CypA (200 ng/mL, b, d, j, l), CypA/CSA (f, n), LPS (1 µg/mL, g, o) or LPS/CSA (h, p) respectively for 1h. The p50 protein of NF-κB activity was analysed by IF with FITC-conjugated anti-p50 antibody (green fluorescence) for the uTHP-1 and Cy3-conjugated anti-p50 antibody (red fluorescence) for the dTHP-1, respectively. CypA-induced nuclear translocation of NF-κB 2 h after treatment (b and h). Nuclear translocation of NF-κB induced by CypA was blockaded by AP-9 (d and l) or CSA (f and n) respectively. ( b ) Merged images of Cy3 and DAPI in the dTHP-1 were obtained using computer software. After CypA stimulating THP-1, the red color for NF-κB overlayed with the blue color for nucleus in cell nucleus under immunofluorescence microscopy, which showed plenty of p50 transducted into nucleus. The scale bars indicate 25 μm ( a ) and 10 μm ( b ). The data shown are representative of similar results from three independent experiments.

    Techniques Used: Activity Assay, Control, Fluorescence, Translocation Assay, Software, Immunofluorescence, Microscopy



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