anti mouse rat cd55 (Bio-Rad)

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Bio-Rad anti mouse rat cd55

See also . (A) mRNA expression fold changes (FC) and p values for complement regulators CD35, CD46, <t>CD55,</t> and CD59 in different colorectal cancer/adenocarcinoma versus normal tissue pairs. Data were acquired from https://www.oncomine.org/resource/login.html (GSE20916) . (B) Relative expression of CD55 (log 10 conversion) in COADREAD patients is shown against hypoxia signature expression (log 10 conversion) . Two-tailed p value is shown for the Pearson r (correlation coefficient). (C) KM curve for colorectal cancer patients with high (red) or low (blue) CD55 mRNA expression levels is shown. This analysis was based on the PrognoScan database ( http://dna00.bio.kyutech.ac.jp/PrognoScan/ ) using the publicly available Gene Expression Omnibus data ( https://www.ncbi.nlm.nih.gov/geo ) with the accession number GSE14333 ( ; ). p = 0.011327. (D) mRNA expression of CD55/18S is shown. qPCR was carried out following exposure of HCT116 cells to 0, 6, or 24 hr of hypoxia (1% O 2 ) or 24 hr of hypoxia followed by 1 hr reoxygenation (21% O 2 ). ** = p value < 0.01, 1-way ANOVA with Tukey’s multiple comparison test. Error bars represent the SEM for a representative experiment. n = 3. (E) HCT116 cells were treated with 0, 6, or 24 hr of hypoxia (1% O 2 ) or 24 hr of hypoxia followed by 1 hr reoxygenation (21% O 2 ). Western blotting (WB) was carried with the antibodies indicated. β-actin, loading control. n = 3. (F) CT26 cells were treated with 0 or 24 hr of hypoxia (1% O 2 ). WB was carried with the antibodies indicated. β-actin, loading control. n = 3. (G) Graph shows the % CMC/total lysis in HCT116 cells treated in 21% O 2 (normoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis (and total cell number) following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3. (H) Graph shows the % CMC/total lysis in HCT116 cells treated with 24 hr of 1% O 2 (hypoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis (and total cell number) following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3 (I) Graph shows the % CMC/total lysis in CT26 cells treated in 21% O 2 (normoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis following treatment with either normal human serum or heat-inactivated normal human serum. * = p value < 0.05, unpaired t test. Error bars represent the SEM for a representative experiment. n = 3. (J) Graph shows the % CMC/total lysis in CT26 cells treated with 24 hr of 1% O 2 (hypoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3.

Anti Mouse Rat Cd55, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/curve+fitting+function+in+sigmaplot+software/Mouse+anti+Human+CD55/pmc06405289-230-11-6
Average 93 stars, based on 61 article reviews

anti mouse rat cd55 - by Bioz Stars, 2026-08

93/100 stars

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1) Product Images from "Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer"

Article Title: Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer

Journal: Cell reports

doi: 10.1016/j.celrep.2018.11.093

See also . (A) mRNA expression fold changes (FC) and p values for complement regulators CD35, CD46, CD55, and CD59 in different colorectal cancer/adenocarcinoma versus normal tissue pairs. Data were acquired from https://www.oncomine.org/resource/login.html (GSE20916) . (B) Relative expression of CD55 (log 10 conversion) in COADREAD patients is shown against hypoxia signature expression (log 10 conversion) . Two-tailed p value is shown for the Pearson r (correlation coefficient). (C) KM curve for colorectal cancer patients with high (red) or low (blue) CD55 mRNA expression levels is shown. This analysis was based on the PrognoScan database ( http://dna00.bio.kyutech.ac.jp/PrognoScan/ ) using the publicly available Gene Expression Omnibus data ( https://www.ncbi.nlm.nih.gov/geo ) with the accession number GSE14333 ( ; ). p = 0.011327. (D) mRNA expression of CD55/18S is shown. qPCR was carried out following exposure of HCT116 cells to 0, 6, or 24 hr of hypoxia (1% O 2 ) or 24 hr of hypoxia followed by 1 hr reoxygenation (21% O 2 ). ** = p value < 0.01, 1-way ANOVA with Tukey’s multiple comparison test. Error bars represent the SEM for a representative experiment. n = 3. (E) HCT116 cells were treated with 0, 6, or 24 hr of hypoxia (1% O 2 ) or 24 hr of hypoxia followed by 1 hr reoxygenation (21% O 2 ). Western blotting (WB) was carried with the antibodies indicated. β-actin, loading control. n = 3. (F) CT26 cells were treated with 0 or 24 hr of hypoxia (1% O 2 ). WB was carried with the antibodies indicated. β-actin, loading control. n = 3. (G) Graph shows the % CMC/total lysis in HCT116 cells treated in 21% O 2 (normoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis (and total cell number) following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3. (H) Graph shows the % CMC/total lysis in HCT116 cells treated with 24 hr of 1% O 2 (hypoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis (and total cell number) following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3 (I) Graph shows the % CMC/total lysis in CT26 cells treated in 21% O 2 (normoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis following treatment with either normal human serum or heat-inactivated normal human serum. * = p value < 0.05, unpaired t test. Error bars represent the SEM for a representative experiment. n = 3. (J) Graph shows the % CMC/total lysis in CT26 cells treated with 24 hr of 1% O 2 (hypoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3.

Figure Legend Snippet: See also . (A) mRNA expression fold changes (FC) and p values for complement regulators CD35, CD46, CD55, and CD59 in different colorectal cancer/adenocarcinoma versus normal tissue pairs. Data were acquired from https://www.oncomine.org/resource/login.html (GSE20916) . (B) Relative expression of CD55 (log 10 conversion) in COADREAD patients is shown against hypoxia signature expression (log 10 conversion) . Two-tailed p value is shown for the Pearson r (correlation coefficient). (C) KM curve for colorectal cancer patients with high (red) or low (blue) CD55 mRNA expression levels is shown. This analysis was based on the PrognoScan database ( http://dna00.bio.kyutech.ac.jp/PrognoScan/ ) using the publicly available Gene Expression Omnibus data ( https://www.ncbi.nlm.nih.gov/geo ) with the accession number GSE14333 ( ; ). p = 0.011327. (D) mRNA expression of CD55/18S is shown. qPCR was carried out following exposure of HCT116 cells to 0, 6, or 24 hr of hypoxia (1% O 2 ) or 24 hr of hypoxia followed by 1 hr reoxygenation (21% O 2 ). ** = p value < 0.01, 1-way ANOVA with Tukey’s multiple comparison test. Error bars represent the SEM for a representative experiment. n = 3. (E) HCT116 cells were treated with 0, 6, or 24 hr of hypoxia (1% O 2 ) or 24 hr of hypoxia followed by 1 hr reoxygenation (21% O 2 ). Western blotting (WB) was carried with the antibodies indicated. β-actin, loading control. n = 3. (F) CT26 cells were treated with 0 or 24 hr of hypoxia (1% O 2 ). WB was carried with the antibodies indicated. β-actin, loading control. n = 3. (G) Graph shows the % CMC/total lysis in HCT116 cells treated in 21% O 2 (normoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis (and total cell number) following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3. (H) Graph shows the % CMC/total lysis in HCT116 cells treated with 24 hr of 1% O 2 (hypoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis (and total cell number) following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3 (I) Graph shows the % CMC/total lysis in CT26 cells treated in 21% O 2 (normoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis following treatment with either normal human serum or heat-inactivated normal human serum. * = p value < 0.05, unpaired t test. Error bars represent the SEM for a representative experiment. n = 3. (J) Graph shows the % CMC/total lysis in CT26 cells treated with 24 hr of 1% O 2 (hypoxia) and either CD55 blocking antibody or IgG control (for the last hour of treatment). % CMC/total lysis was assessed by calculating calcein release/total lysis following treatment with either normal human serum or heat-inactivated normal human serum. **** = p value < 0.0001, unpaired t test, two-tailed. Error bars represent the SEM for a representative experiment. n = 3.

Techniques Used: Expressing, Two Tailed Test, Western Blot, Lysis, Blocking Assay

Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Blocking Assay, Viability Assay, Recombinant, shRNA, Software

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anti mouse rat cd55 (Bio-Rad)

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1) Product Images from "Mutations in an Innate Immunity Pathway Are Associated with Poor Overall Survival Outcomes and Hypoxic Signaling in Cancer"

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