Journal: Molecular Biology of the Cell
Article Title: Lymphatic endothelial cell calcium pulses are sensitive to spatial gradients in wall shear stress
doi: 10.1091/mbc.E18-10-0618
Figure Lengend Snippet: HLMVEC Ca 2+ pulse durations are sensitive to WSS magnitude and WSSGs in cells that pulse repeatedly. Plots represent the cumulative distribution functions (CDFs) for individual pulse durations (measured as FWHM) for P cells. The initial pulse (first pulse, FP) is shown in red, while all subsequent pulses (second, third, etc., SP) are shown in black. Dotted lines are fits to the Weibull distribution (see text). Pulse duration distributions are portrayed for Rings 1–6 (A–F), spatially uniform WSS (parallel plate chamber, G), and no flow (H). The scale (β) and shape (κ) parameters for fits to the Weibull distribution and the average pulse durations (FWHM; μ) are presented at the bottom of each graph. Experiments were performed in triplicate. Asterisks indicate statistically significant differences in CDFs as determined using the Kolmogorov–Smirnov test ( n.s. , not significant; *, p < 0.05; **, p < 10 –4 ; ***, p < 10 –7 ).
Article Snippet: Cumulative distribution function plots and modeling of the distributions of experimental data were performed using MATLAB.
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