ipilimumab anti ctla 4 ctla 4 inhibitor  (MedChemExpress)

Journal: Nature Communications

Article Title: Distinct migratory dendritic cell subsets cooperate to prime tissue-resident memory-like CD8⁺ T cells in mouse tumors

doi: 10.1038/s41467-026-77641-7

Figure Lengend Snippet: A – E scRNAseq of 80% T Act and 20% T CM /T Naive sorted from the tdLN of 14-day KP-bearing mice (gating strategy in Supplementary Fig. ). A UMAP dimensionality reduction embedding the different clusters. B Dot plot showing the expression of core marker genes across clusters. C Violin plots showing the scoring of indicated gene signatures across activated clusters , , . D Distribution of T Act clusters across CITE-Seq gated CD103 - T Act and CD103 + T Act . E Projection of lung Cd44 + Sell - scRNA-seq clusters 1, 4, 7, 8, and 9 on tdLN T Act clusters 0, 3, 5, and 8. F Representative flow cytometry gating strategy and quantification of the proportions of T Act populations in KP tdLN ( n = 12 mice). Data are presented as stacked mean values ± SD. G Representative flow cytometry histograms and quantifications of SLAMF6, CD49a, and Hobit-tdTomato expression across tdLN T Act subsets ( n = 5 Hobit-tdTomato mice, repeated measures one-way ANOVA with Tukey’s multiple comparisons test). Data are presented as mean values ± SD with individual replicates displayed. H Representative flow cytometry gating strategy showing the phenotype of tetramer-OVA + (dark) or tetramer-OVA - cells (grey) in the tdLN of KP-OVA tumor-bearing mice. I Quantification of the proportions of Tet-OVA + or Tet-OVA - CD8 + T cell populations in the tdLN of KP-OVA tumor-bearing mice ( n = 4 mice, repeated measures two-way ANOVA with Šídák’s multiple comparisons test). Data are presented as stacked mean values ± SD. J Quantification of the absolute numbers of Tet-OVA + or Tet-OVA - CD8 + T cell populations in the tdLN of KP-OVA tumor-bearing mice ( n = 4 mice, repeated measures two-way ANOVA with Šídák’s multiple comparisons test). Data are presented as mean values ± SD with individual replicates displayed. K Representative flow cytometry plots showing tdLN Tet-OVA + T Act subsets at day 17 post KP-OVA tumor injection and chronic isotype, anti-PD-1, anti-CTLA-4, or combined treatment as depicted in Supplementary Fig. . L Quantifications of the proportions of tdLN Tet-OVA + T Act subsets at day 17 post KP-OVA tumor injection and chronic isotype, anti-PD-1, anti-CTLA-4, or combined treatment as depicted in Supplementary Fig. ( n = 7 mice per group, one-way ANOVA with Tukey’s multiple comparisons test). Data are presented as mean values ± SD with individual replicates displayed. KP: ( Kras G12D , p53 -/- ) lung adenocarcinoma; tdLN: tumor-draining lymph node; T Act : activated CD8 + T cell; T RM : tissue-resident memory-like CD8 + T cell; T Ex : exhausted-like CD8 + T cell; T Effector : effector-like CD8 + T cell.

Article Snippet: For chronic ICB immunotherapies, mice received intraperitoneal injections of 200 μg control isotype (clone 2A3, BioXCell BE0089), anti-CTLA-4 (clone 9H10, BioXCell BE0131), anti-PD-1 (clone 29F.1A12, BioxCell BE0273), or both anti-PD-1 and anti-CTLA-4 antibodies in 200 μl of PBS every 3 days from day 7 after KP-OVA tumor injection and mice were euthanized at day 17 for T cell analysis.

Techniques: Expressing, Marker, Flow Cytometry, Injection, Tissue

Journal: Advanced Science

Article Title: Coixenolide Enhances Antitumor Immunity of Cytotoxic CD8 + T Cells by Inhibiting S100A8/A9‐CD36 Axis in Obesity‐Associated Pancreatic Adenocarcinoma

doi: 10.1002/advs.202515759

Figure Lengend Snippet: Cell–cell communication analysis indicated that S100a8/a9 served as a critical target for coixenolide, regulating the interactions between PAAD cells and CD8 + T cells. (A) The overview of cell–cell interactions between malignant epithelial cells and T cells in the tumor tissues treated with vehicle control and coixenolide, respectively. (B) The intensity of intercellular signaling, showing both the sent and received signals. The cells in the upper right corner exhibit the strongest incoming and outgoing interactions. S100a8/a9 + tumor cell clusters (Clusters 2 and 8) are highlighted in red boxes. Cytotoxic CD8 + T and Treg cells are indicated by red arrows. (C) The outgoing communication patterns of epithelial cells and T cells in the tumor of HFD‐fed mice with or without coixenolide treatment. (D) CD8 + T cell killing assays were conducted using coculture of Pan02 and CD8 + T cells. Pan02 or CD8 + T cells were pretreated with 25 mg/mL coixenolide before coculture. (E) Primary CD8 + T cells were activated by incubation with CD3/CD28 antibody and labeled with carboxy‐fluorescein succinimidyl ester (CFSE). (F–H) The levels of TNF‐α (F), IFN‐γ (G), and Granzyme B (GZMB) (H) in the supernatants after co‐culture of CD8 + T and Pan02 cells pretreated with coixenolide (+) or vehicle control (−) were determined using ELISA. (I–K) The expression of TNF‐α (I), IFN‐γ (J), and Granzyme B (GZMB) (K) in CD8 + T cells after co‐culture of CD8 + T cells and Pan02 cells pretreated with coixenolide (+) or vehicle control (−) was determined using flow cytometry. (L) Flow cytometry analyses of T cell exhaustion markers (CTLA‐4) on CD8 + T cells after coculture of Pan02 and CD8 + T cells pretreated with coixenolide (+) or vehicle control (−). (M,N) Flow cytometry analyses of CD8 + T cell killing (M) and division (N) activities. CD8 + T cells were cocultured with S100a9 knockout (KO) or wild‐type (WT) Pan02 cells. (O–Q) The levels of TNF‐α (O), IFN‐γ (P), and GZMB (Q) in the supernatants after coculture of CD8 + T cells with WT or S100a9 KO Pan02 cells were determined using ELISA. n = 3 or 4 independent coculture experiments or replicates. One‐way ANOVA with Tukey's post‐hoc test (E–L), two‐sided t ‐test (M–N), or two‐way ANOVA (O‐Q) was used. All p values are two‐sided. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following treatment with coixenolide for 24 h, Treg cells were stained with PE‐conjugated anti‐mouse CTLA‐4 antibody ( A27774 , ABclonal) and FITC‐conjugated anti‐mouse IL‐10 antibody ( A27680 , ABclonal), and the expression levels of CTLA‐4 and IL‐10 were analyzed by flow cytometry.

Techniques: Control, Incubation, Labeling, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Knock-Out