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easyseptm direct human ctc enrichment kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc easyseptm direct human ctc enrichment kit
    Easyseptm Direct Human Ctc Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctc+kit/easyseptm+direct+human+ctc+enrichment+kit/pm40639576-69-5-11
    Average 90 stars, based on 1 article reviews
    easyseptm direct human ctc enrichment kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancer.
    Article Snippet: The EasySepTM Direct Human CTC Enrichment Kit (#19657; StemCell Technologies) along with the “Big Easy” Magnet (#18001; StemCell Technologies, Molecular Oncology (2024) a 2024 The Author(s).

    Article Title: Liquid biopsy yield enhancement
    Article Snippet: An exemplary system for CTC enrichment includes the EasySepTM Direct Human CTC Enrichment (StemCell Technologies), and CellSearch® (Veridex, LLC, Raritan, N.J., USA).

    Labeling:

    Article Title: Design of buffer property for the new enrichment method of circulating tumor cell based on immunomagnetic-negative separation
    Article Snippet: The fluorescently labeled A549 cells were spiked into a tube containing 6 mL of the separation buffer, which was further supplemented with 100 μL of human FcR Blocking Reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated for 10 min. .. Upon spiking the labeled A549 cells into 6 mL of the blood sample diluted with 6 mL of the separation buffer, an additional 300 μL of the EasySepTM Direct Human CTC Enrichment Cocktail (STEMCELL Technologies) was additionally added to the reaction tube and further incubated for 5 min. .. Subsequently, 300 μL of EasySepTM Direct RapidSpheresTM 50300 magnetic beads (STEMCELL Technologies) were added to the reaction tube and incubated for 10 min.

    Incubation:

    Article Title: Design of buffer property for the new enrichment method of circulating tumor cell based on immunomagnetic-negative separation
    Article Snippet: The fluorescently labeled A549 cells were spiked into a tube containing 6 mL of the separation buffer, which was further supplemented with 100 μL of human FcR Blocking Reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) and incubated for 10 min. .. Upon spiking the labeled A549 cells into 6 mL of the blood sample diluted with 6 mL of the separation buffer, an additional 300 μL of the EasySepTM Direct Human CTC Enrichment Cocktail (STEMCELL Technologies) was additionally added to the reaction tube and further incubated for 5 min. .. Subsequently, 300 μL of EasySepTM Direct RapidSpheresTM 50300 magnetic beads (STEMCELL Technologies) were added to the reaction tube and incubated for 10 min.

    Selection:

    Article Title: Genomic characteristics and clinical significance of CD56+ circulating tumor cells in small cell lung cancer.
    Article Snippet: .. The first step consists in a CTC enrichment from whole blood sample by immunomagnetic negative selection using the EasySepTM Direct Human CTC Enrichment Kit (StemCell Technologies®, Vancouver, Canada) according to manufacturer instruction. ..



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    Image Search Results


    (A) Representative immunofluorescence images of CTCs with different extraction methods. Co-localization of STING (green, Alexa Fluor 488) and vimentin (red, Alexa Fluor 647). Nuclei were stained with DAPI (blue). Magnification 63X. (B) Representative SEM images of the isolated exosomes (scale bar = 2 μm). (C) Western blot of exosomal markers CD81 and CD63. The isolated exosomes with different protein concentrations (EXO#1 = 40 μg and EXO#2 = 20 μg) were successfully obtained from the culture supernatants of PBMCs from SCLC patients. Red line indicated the band of each protein on the gel. Original western blots are presented in File S1. (D) Stacked bar plots showing the log10-transformed positive control read counts across the samples. (E) Violin plot displaying the distribution of the log of the un-normalized gene counts for all the samples.

    Journal: The Journal of Liquid Biopsy

    Article Title: Novel applications of liquid Biopsy: Comprehensive methodology for circulating biomarker exploration in peripheral blood

    doi: 10.1016/j.jlb.2025.100307

    Figure Lengend Snippet: (A) Representative immunofluorescence images of CTCs with different extraction methods. Co-localization of STING (green, Alexa Fluor 488) and vimentin (red, Alexa Fluor 647). Nuclei were stained with DAPI (blue). Magnification 63X. (B) Representative SEM images of the isolated exosomes (scale bar = 2 μm). (C) Western blot of exosomal markers CD81 and CD63. The isolated exosomes with different protein concentrations (EXO#1 = 40 μg and EXO#2 = 20 μg) were successfully obtained from the culture supernatants of PBMCs from SCLC patients. Red line indicated the band of each protein on the gel. Original western blots are presented in File S1. (D) Stacked bar plots showing the log10-transformed positive control read counts across the samples. (E) Violin plot displaying the distribution of the log of the un-normalized gene counts for all the samples.

    Article Snippet: MACS® Tumor Cell Isolation Kit depletes tumor-associated cells without harming CTCs; we modified the protocol by omitting Non-Tumor Cell Depletion Cocktail B to preserve mesenchymal CTCs (Order no. 130-108-339, www.miltenyibiotec.com ).

    Techniques: Immunofluorescence, Extraction, Staining, Isolation, Western Blot, Transformation Assay, Positive Control