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bacstain ctc rapid staining kit  (Dojindo Labs)


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    Structured Review

    Dojindo Labs bacstain ctc rapid staining kit
    Bacstain Ctc Rapid Staining Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctc+kit/Bacstain+CTC+Rapid+Staining+Kit/pm25626919-87-47-52
    Average 93 stars, based on 36 article reviews
    bacstain ctc rapid staining kit - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Fabrication of cyborg bacterial cells as living cell-material hybrids using intracellular hydrogelation.
    Article Snippet: The production of living therapeutics, cell-based delivery of drugs and gene-editing tools and the manufacturing of bio-commodities all share a common concept: they use either a synthetic or a living cell chassis to achieve their primary engineering or therapeutic goal.. Live-cell chassis face limitations inherent to their auto-replicative nature and the complexity of the cellular context.. This limitation highlights the need for a new chassis combining the engineering simplicity of synthetic materials and the functionalities of natural cells.

    Article Title: Effect of Humidity on Antibacterial Activity of Copper and Its Alloy Surfaces
    Article Snippet: .. The viable bacterial number was decided using 5-cyano2,3-ditolyl-2H-tetrazolium chloride (CTC) staining kit (-Bacstain- CTC Rapid Staining Kit for Flow cytometry, BS01, Dojindo Laboratories, Kumamoto, Japan) following its instruction. ..

    Article Title: Quantitative Evaluation of Nucleic Acid Degradability of Copper Alloy Surfaces and Its Correlation to Antibacterial Activity
    Article Snippet: .. Then, a 200 μL portion was used for a viable cell count using 5-cyano-2,3-ditolyl-2H-tetrazolium chloride (CTC) staining kit (-Bacstain- CTC Rapid Staining Kit for Flow cytometry, BS01, Dojindo Laboratories, Kumamoto, Japan) following its instruction. ..

    Article Title: Efficient ε-poly-L-lysine production by Streptomyces albulus based on a dynamic pH-regulation strategy
    Article Snippet: Previous studies have demonstrated that the biosynthesis of ε-poly-L-lysine (ε-PL) is suppressed due to the decrease in cell viability in the late phase of fed-batch fermentation.. To address this, a dynamic pH-regulation strategy was developed to maintain and improve ε-PL production.. Using the method proposed here, ε-PL production and productivity reached 60.2 g/L and 7.5 g/L/d, respectively, both of which were 36.5% higher compared to the case for the fermentation without dynamic pH regulation.

    Article Title: Optimization of Spore Purification and Quality Assessment in Paenibacillus Species.
    Article Snippet: .. Briefly, spore samples (1.0 × 106 cells/mL, i.e., 6.0 log CFU/mL) were stained using the Bacstain CTC Rapid Staining Kit (Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions and incubated at 37 °C for 30 min in the dark. .. The stained samples were analyzed using a Guava easyCyte flow cytometer (Cytek Industries, Woodland Park, NJ, USA), and the data were processed with FlowJo v10.10.0 (BD Biosciences, Bristol, United Kingdom).

    Article Title: Cannabis and derivatives thereof for the treatment of pain and inflammation related with dental pulp and bone regeneration related to dental jaw bone defects
    Article Snippet: .. The samples were stained with Dojindo Rapid staining kit BS02 and evaluated under a Cytation 5 cell imaging microscope. ..

    Flow Cytometry:

    Article Title: Fabrication of cyborg bacterial cells as living cell-material hybrids using intracellular hydrogelation.
    Article Snippet: The production of living therapeutics, cell-based delivery of drugs and gene-editing tools and the manufacturing of bio-commodities all share a common concept: they use either a synthetic or a living cell chassis to achieve their primary engineering or therapeutic goal.. Live-cell chassis face limitations inherent to their auto-replicative nature and the complexity of the cellular context.. This limitation highlights the need for a new chassis combining the engineering simplicity of synthetic materials and the functionalities of natural cells.

    Article Title: Effect of Humidity on Antibacterial Activity of Copper and Its Alloy Surfaces
    Article Snippet: .. The viable bacterial number was decided using 5-cyano2,3-ditolyl-2H-tetrazolium chloride (CTC) staining kit (-Bacstain- CTC Rapid Staining Kit for Flow cytometry, BS01, Dojindo Laboratories, Kumamoto, Japan) following its instruction. ..

    Article Title: Quantitative Evaluation of Nucleic Acid Degradability of Copper Alloy Surfaces and Its Correlation to Antibacterial Activity
    Article Snippet: .. Then, a 200 μL portion was used for a viable cell count using 5-cyano-2,3-ditolyl-2H-tetrazolium chloride (CTC) staining kit (-Bacstain- CTC Rapid Staining Kit for Flow cytometry, BS01, Dojindo Laboratories, Kumamoto, Japan) following its instruction. ..

    Cell Counting:

    Article Title: Quantitative Evaluation of Nucleic Acid Degradability of Copper Alloy Surfaces and Its Correlation to Antibacterial Activity
    Article Snippet: .. Then, a 200 μL portion was used for a viable cell count using 5-cyano-2,3-ditolyl-2H-tetrazolium chloride (CTC) staining kit (-Bacstain- CTC Rapid Staining Kit for Flow cytometry, BS01, Dojindo Laboratories, Kumamoto, Japan) following its instruction. ..

    Activity Assay:

    Article Title: Efficient ε-poly-L-lysine production by Streptomyces albulus based on a dynamic pH-regulation strategy
    Article Snippet: Previous studies have demonstrated that the biosynthesis of ε-poly-L-lysine (ε-PL) is suppressed due to the decrease in cell viability in the late phase of fed-batch fermentation.. To address this, a dynamic pH-regulation strategy was developed to maintain and improve ε-PL production.. Using the method proposed here, ε-PL production and productivity reached 60.2 g/L and 7.5 g/L/d, respectively, both of which were 36.5% higher compared to the case for the fermentation without dynamic pH regulation.

    Concentration Assay:

    Article Title: Efficient ε-poly-L-lysine production by Streptomyces albulus based on a dynamic pH-regulation strategy
    Article Snippet: Previous studies have demonstrated that the biosynthesis of ε-poly-L-lysine (ε-PL) is suppressed due to the decrease in cell viability in the late phase of fed-batch fermentation.. To address this, a dynamic pH-regulation strategy was developed to maintain and improve ε-PL production.. Using the method proposed here, ε-PL production and productivity reached 60.2 g/L and 7.5 g/L/d, respectively, both of which were 36.5% higher compared to the case for the fermentation without dynamic pH regulation.

    ATP Assay:

    Article Title: Efficient ε-poly-L-lysine production by Streptomyces albulus based on a dynamic pH-regulation strategy
    Article Snippet: Previous studies have demonstrated that the biosynthesis of ε-poly-L-lysine (ε-PL) is suppressed due to the decrease in cell viability in the late phase of fed-batch fermentation.. To address this, a dynamic pH-regulation strategy was developed to maintain and improve ε-PL production.. Using the method proposed here, ε-PL production and productivity reached 60.2 g/L and 7.5 g/L/d, respectively, both of which were 36.5% higher compared to the case for the fermentation without dynamic pH regulation.

    Incubation:

    Article Title: Optimization of Spore Purification and Quality Assessment in Paenibacillus Species.
    Article Snippet: .. Briefly, spore samples (1.0 × 106 cells/mL, i.e., 6.0 log CFU/mL) were stained using the Bacstain CTC Rapid Staining Kit (Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions and incubated at 37 °C for 30 min in the dark. .. The stained samples were analyzed using a Guava easyCyte flow cytometer (Cytek Industries, Woodland Park, NJ, USA), and the data were processed with FlowJo v10.10.0 (BD Biosciences, Bristol, United Kingdom).

    Imaging:

    Article Title: Cannabis and derivatives thereof for the treatment of pain and inflammation related with dental pulp and bone regeneration related to dental jaw bone defects
    Article Snippet: .. The samples were stained with Dojindo Rapid staining kit BS02 and evaluated under a Cytation 5 cell imaging microscope. ..

    Microscopy:

    Article Title: Cannabis and derivatives thereof for the treatment of pain and inflammation related with dental pulp and bone regeneration related to dental jaw bone defects
    Article Snippet: .. The samples were stained with Dojindo Rapid staining kit BS02 and evaluated under a Cytation 5 cell imaging microscope. ..



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    Image Search Results


    (A) Representative immunofluorescence images of CTCs with different extraction methods. Co-localization of STING (green, Alexa Fluor 488) and vimentin (red, Alexa Fluor 647). Nuclei were stained with DAPI (blue). Magnification 63X. (B) Representative SEM images of the isolated exosomes (scale bar = 2 μm). (C) Western blot of exosomal markers CD81 and CD63. The isolated exosomes with different protein concentrations (EXO#1 = 40 μg and EXO#2 = 20 μg) were successfully obtained from the culture supernatants of PBMCs from SCLC patients. Red line indicated the band of each protein on the gel. Original western blots are presented in File S1. (D) Stacked bar plots showing the log10-transformed positive control read counts across the samples. (E) Violin plot displaying the distribution of the log of the un-normalized gene counts for all the samples.

    Journal: The Journal of Liquid Biopsy

    Article Title: Novel applications of liquid Biopsy: Comprehensive methodology for circulating biomarker exploration in peripheral blood

    doi: 10.1016/j.jlb.2025.100307

    Figure Lengend Snippet: (A) Representative immunofluorescence images of CTCs with different extraction methods. Co-localization of STING (green, Alexa Fluor 488) and vimentin (red, Alexa Fluor 647). Nuclei were stained with DAPI (blue). Magnification 63X. (B) Representative SEM images of the isolated exosomes (scale bar = 2 μm). (C) Western blot of exosomal markers CD81 and CD63. The isolated exosomes with different protein concentrations (EXO#1 = 40 μg and EXO#2 = 20 μg) were successfully obtained from the culture supernatants of PBMCs from SCLC patients. Red line indicated the band of each protein on the gel. Original western blots are presented in File S1. (D) Stacked bar plots showing the log10-transformed positive control read counts across the samples. (E) Violin plot displaying the distribution of the log of the un-normalized gene counts for all the samples.

    Article Snippet: MACS® Tumor Cell Isolation Kit depletes tumor-associated cells without harming CTCs; we modified the protocol by omitting Non-Tumor Cell Depletion Cocktail B to preserve mesenchymal CTCs (Order no. 130-108-339, www.miltenyibiotec.com ).

    Techniques: Immunofluorescence, Extraction, Staining, Isolation, Western Blot, Transformation Assay, Positive Control