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crop seq vector  (Addgene inc)


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    Structured Review

    Addgene inc crop seq vector
    Crop Seq Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc09352581-317-10-13
    Average 96 stars, based on 124 article reviews
    crop seq vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Splice isoform-perturbation coupled to single cell transcriptome profiling reveals functions of microexons in neurogenesis and autism-linked pathways
    Article Snippet: Cells were passaged using Trypsin (Life Technologies) and maintained at subconfluent conditions at 37 °C and 5% CO2.

    Modification:

    Article Title: MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy.
    Article Snippet: .. Plasmids and gRNA library construction We modified CROPseq-guide-Puro (Addgene, plasmid 86708) lentiviral plasmid22 to embed the gRNA sequences in the 3′ UTR of a polyadenylated RFP transcript, generating the CROPseq–RFP lentiviral vector. ..

    Plasmid Preparation:

    Article Title: MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy.
    Article Snippet: .. Plasmids and gRNA library construction We modified CROPseq-guide-Puro (Addgene, plasmid 86708) lentiviral plasmid22 to embed the gRNA sequences in the 3′ UTR of a polyadenylated RFP transcript, generating the CROPseq–RFP lentiviral vector. ..

    Article Title: X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping
    Article Snippet: .. CROPseq-Guide-puro plasmid (Addgene; #86708) was digested with MluI-HF (NEB; #R3198S) and gel-purified. .. The oligos were annealed, PCR-amplified using Q5 High-Fidelity PCR (NEB; #E0555L) and gel purified.

    Article Title: X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping
    Article Snippet: 1 μg of CROPseq-Guide-puro (Addgene; #86708) was digested with SnaBI (NEB; #R0130S) and SalI-HF (NEB; #R3138S) and gel purified. .. The filler and the LTR fragments were generated through PCR from the CROPseq-Guide-puro plasmid (Addgene; #86708) and gel purified. .. The Gibson assembly was performed according to the NEBuilder HiFi DNA Assembly Cloning Kit instructions in a 1:5 ratio.

    Generated:

    Article Title: X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping
    Article Snippet: 1 μg of CROPseq-Guide-puro (Addgene; #86708) was digested with SnaBI (NEB; #R0130S) and SalI-HF (NEB; #R3138S) and gel purified. .. The filler and the LTR fragments were generated through PCR from the CROPseq-Guide-puro plasmid (Addgene; #86708) and gel purified. .. The Gibson assembly was performed according to the NEBuilder HiFi DNA Assembly Cloning Kit instructions in a 1:5 ratio.

    Polymerase Chain Reaction:

    Article Title: X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping
    Article Snippet: 1 μg of CROPseq-Guide-puro (Addgene; #86708) was digested with SnaBI (NEB; #R0130S) and SalI-HF (NEB; #R3138S) and gel purified. .. The filler and the LTR fragments were generated through PCR from the CROPseq-Guide-puro plasmid (Addgene; #86708) and gel purified. .. The Gibson assembly was performed according to the NEBuilder HiFi DNA Assembly Cloning Kit instructions in a 1:5 ratio.

    Purification:

    Article Title: X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping
    Article Snippet: 1 μg of CROPseq-Guide-puro (Addgene; #86708) was digested with SnaBI (NEB; #R0130S) and SalI-HF (NEB; #R3138S) and gel purified. .. The filler and the LTR fragments were generated through PCR from the CROPseq-Guide-puro plasmid (Addgene; #86708) and gel purified. .. The Gibson assembly was performed according to the NEBuilder HiFi DNA Assembly Cloning Kit instructions in a 1:5 ratio.

    Article Title: X-CODE: a dual RNA barcoding system for multi-platform clonal tracking and spatial phenotyping
    Article Snippet: .. 1 μg of CROPseq-Guide-puro (Addgene; #86708) was digested with SnaBI (NEB; #R0130S) and SalI-HF (NEB; #R3138S) and gel purified. .. The filler and the LTR fragments were generated through PCR from the CROPseq-Guide-puro plasmid (Addgene; #86708) and gel purified.

    Infection:

    Article Title: Exonic enhancers are a widespread class of dual-function regulatory elements.
    Article Snippet: .. Single cells were seeded into 96-well plates were subsequently infected with a Crop-seq-derived lentivirus (Addgene #86708) encoding an sgRNA targeting the human CD81 promoter (Supplementary Table 7), followed by seven days of puromycin selection. ..

    Selection:

    Article Title: Exonic enhancers are a widespread class of dual-function regulatory elements.
    Article Snippet: .. Single cells were seeded into 96-well plates were subsequently infected with a Crop-seq-derived lentivirus (Addgene #86708) encoding an sgRNA targeting the human CD81 promoter (Supplementary Table 7), followed by seven days of puromycin selection. ..



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    Image Search Results


    a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Journal: Nature Methods

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing

    doi: 10.1038/s41592-025-02805-0

    Figure Lengend Snippet: a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Article Snippet: The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA .

    Techniques: Binding Assay, Functional Assay, Flow Cytometry