biotinylated antisense crna with the enzo bioarray high yield rna transcript labelling kit (Bioarray Inc)

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Bioarray Inc biotinylated antisense crna with the enzo bioarray high yield rna transcript labelling kit
Biotinylated Antisense Crna With The Enzo Bioarray High Yield Rna Transcript Labelling Kit, supplied by Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crna+transcript+labeling+kit/biotin+labeled+crna/pmc06495815-75-9-15
Average 90 stars, based on 1 article reviews

biotinylated antisense crna with the enzo bioarray high yield rna transcript labelling kit - by Bioz Stars, 2026-11

90/100 stars

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Generated:

Article Title: Spatial Differentiation in the Vegetative Mycelium of Aspergillus niger
Article Snippet: The quality of the RNA was analyzed with an Agilent 2100 bioanalyzer, using an RNA6000 LabChip kit (Agilent Technology, Palo Alto, CA). .. Biotin-labeled antisense cRNA was generated by labeling 20 or 2 μg of total RNA with a BioArray high-yield RNA transcription labeling kit (ENZO) or an Affymetrix eukaryotic one-cycle target labeling and control reagent package, respectively. .. The quality of the cRNA was checked using the Agilent 2100 bioanalyzer.

Article Title: CD8+ T regulatory cells use a novel genetic program that includes CD103 to suppress Th1 immunity in eye-derived tolerance.
Article Snippet: METHODS.. Ovalbumin (OVA)-specific CD8 T cells from T-cell receptor (TCR) transgenic OT-1 mice acquire efferent regulatory properties similar to in vivo–generated CD8 T regs after stimulation with OVA-pulsed TGF2-treated APCs.. Changes in the genetic program associated with acquisition of efferent regulatory function in OT-1 CD8 T cells in vitro were determined by DNA microarray analyses and confirmed by RT-PCR analyses and biological assays.

Labeling:

Article Title: Transcript-specific translational regulation in the unfolded protein response of Saccharomyces cerevisiae.
Article Snippet: Instead double-stranded cDNA products were immediately purified following the ‘‘Phase Lock Gel Cleanup of Double-Stranded cDNA’’ protocol (Affymetrix Manual II). cDNA was resuspended in 22 ll of RNAse free water. cRNA preparation was performed with minor modifications to the Affymetrix Manual II. .. Briefly, 11 ll of cDNA was used as a template to produce biotinylated cRNA using half the recommended volumes of the ENZO BioArray High Yield RNA Transcript Labeling Kit. .. Labelled cRNAs were purified following the ‘‘Qiagen RNeasy Cleanup and Quantification of Biotin-Labeled cRNA’’ protocol (Affymetrix Manual II). cRNA quality was assessed on Agilent RNA6000nano LabChips (Agilent Technology 2100 Bioanalyzer Version A.01.20 SI211). cRNA quantity was assessed by running 1 ll of each cRNA sample on a NanoDrop ND-1000 UV–Vis Spectrophotometer (Nanodrop Technologies).

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other:

Article Title: Comparative gene expression of intestinal metabolizing enzymes.
Article Snippet: Biotin-labeled cRNA was synthesized from the double strand cDNA using T7 RNA polymerasecatalysed in vitro transcription in the presence of biotin-labeled NTP (BioArray high yield RNA transcription labeling kit, Enzo Biochem, New York, NY) at 371C for 5 h, and the labeled cRNA was purified using RNease mini kit (Qiagen).

Article Title: Analysis of daily and circadian gene expression in the rat pineal gland
Article Snippet: After extraction with phenol-chloroform and ethanol precipitation with ammonium acetate, the cDNA was used as a template to generate biotin-labeled cRNA probe (Enzo Bioarray, Affymetrix, Santa Clara, CA, USA).

Article Title: Subchronic toxicity and toxicogenomic evaluation of tamoxifen citrate + bexarotene in female rats.
Article Snippet: Purified cDNA was used to synthesize biotin-labeled antisense cRNA using the BioArray High Yield RNA Transcript Labeling Kit. cRNA was fragmented, added to temperature equilibrated, prewetted probe arrays, and hybridized.

Article Title: Transcriptional profiling of Medicago truncatula meristematic root cells
Article Snippet: Biotin-labelled cRNA was generated using the Enzo BioArray kit (Affymetrix), purified using RNeasy spin columns (Qiagen), and then quantified by spectrophotometer.

Article Title: Copper Induces Apoptosis of Neuroblastoma Cells Via Post-translational Regulation of the Expression of Bcl-2-family Proteins and the tx J Mouse is a Better Model of Hepatic than Brain Cu Toxicity
Article Snippet: Details of each sample including the concentration of Cu, glycine and the time point at which the sample was collected is listed in . cDNA (10 μl) was then used to synthesize biotin-labeled cRNA as per the protocol described by the Enzo BioArray High Yield RNA Transcript Labeling Kit (Farmingdale, NY, USA).

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