Review



cpsf5  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Proteintech cpsf5
    3’ UTRs are designated shortened (blue) for Compositional Fold Change (cFC) < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. b, Tracks show read coverage of TGFBR1 in RNA-Seq data from CPSF6 knock-out and NT control cells from one representative biological replicate (donor E), visualized using Integrative Genomics Visualizer. 3’ UTRs show evidence of shortening in response to CPSF6 knock-out. c, Charts show functional enrichment (Metascape) analysis of top pathways enriched in genes with shortened 3’ UTRs (left, blue) and lengthened 3’ UTRs (right, red) in response to CSPF6 knock-out in primary CD4+ T cells as assessed by REPAC analysis in 3 biological replicates (donors E-G). d, Violin plot shows comparison of cFC values from REPAC analysis in all tested genes as compared to genes in the indicated pathways (GO:0042110:T Cell activation, R-HSA-1280215: Cytokine Signaling in Immune system, R-HSA-913531: Interferon Signaling) in 3 biological replicates (donors E-G). e, Scatterplot shows cFC of all tested APA sites in REPAC analysis of CPSF6 knock-out primary CD4+ T cells in as compared to NT controls plotted against differential expression data as shown in in data aggregated from 3 biological replicates (donors E-G). 3’ UTRs are designated shortened (blue) for cFC < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. f, Immunoblot shows knock-out of <t>CPSF5</t> in protein lysates harvested at day 4 post-editing in 3 biological replicates (donor Z-AB). g, HIV-1 infectivity (% GFP positive cells normalized to NT control per donor) at day 5 post-challenge with HIV-1 NL4-3 nef:IRES:GFP in indicated knock-out primary CD4+ T cells from 3 biological replicates (donors Z-AB) as assessed by flow cytometry. Each bar represents the average of technical triplicates + /- SD with individual data points shown. Statistics were calculated relative to the NT control per condition by one-way ANOVA with Dunnet’s test for multiple comparisons; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.
    Cpsf5, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/NUDT21+Antibody/pmc12714223-396-17-23
    Average 94 stars, based on 58 article reviews
    cpsf5 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells"

    Article Title: Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1013745

    3’ UTRs are designated shortened (blue) for Compositional Fold Change (cFC) < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. b, Tracks show read coverage of TGFBR1 in RNA-Seq data from CPSF6 knock-out and NT control cells from one representative biological replicate (donor E), visualized using Integrative Genomics Visualizer. 3’ UTRs show evidence of shortening in response to CPSF6 knock-out. c, Charts show functional enrichment (Metascape) analysis of top pathways enriched in genes with shortened 3’ UTRs (left, blue) and lengthened 3’ UTRs (right, red) in response to CSPF6 knock-out in primary CD4+ T cells as assessed by REPAC analysis in 3 biological replicates (donors E-G). d, Violin plot shows comparison of cFC values from REPAC analysis in all tested genes as compared to genes in the indicated pathways (GO:0042110:T Cell activation, R-HSA-1280215: Cytokine Signaling in Immune system, R-HSA-913531: Interferon Signaling) in 3 biological replicates (donors E-G). e, Scatterplot shows cFC of all tested APA sites in REPAC analysis of CPSF6 knock-out primary CD4+ T cells in as compared to NT controls plotted against differential expression data as shown in in data aggregated from 3 biological replicates (donors E-G). 3’ UTRs are designated shortened (blue) for cFC < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. f, Immunoblot shows knock-out of CPSF5 in protein lysates harvested at day 4 post-editing in 3 biological replicates (donor Z-AB). g, HIV-1 infectivity (% GFP positive cells normalized to NT control per donor) at day 5 post-challenge with HIV-1 NL4-3 nef:IRES:GFP in indicated knock-out primary CD4+ T cells from 3 biological replicates (donors Z-AB) as assessed by flow cytometry. Each bar represents the average of technical triplicates + /- SD with individual data points shown. Statistics were calculated relative to the NT control per condition by one-way ANOVA with Dunnet’s test for multiple comparisons; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.
    Figure Legend Snippet: 3’ UTRs are designated shortened (blue) for Compositional Fold Change (cFC) < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. b, Tracks show read coverage of TGFBR1 in RNA-Seq data from CPSF6 knock-out and NT control cells from one representative biological replicate (donor E), visualized using Integrative Genomics Visualizer. 3’ UTRs show evidence of shortening in response to CPSF6 knock-out. c, Charts show functional enrichment (Metascape) analysis of top pathways enriched in genes with shortened 3’ UTRs (left, blue) and lengthened 3’ UTRs (right, red) in response to CSPF6 knock-out in primary CD4+ T cells as assessed by REPAC analysis in 3 biological replicates (donors E-G). d, Violin plot shows comparison of cFC values from REPAC analysis in all tested genes as compared to genes in the indicated pathways (GO:0042110:T Cell activation, R-HSA-1280215: Cytokine Signaling in Immune system, R-HSA-913531: Interferon Signaling) in 3 biological replicates (donors E-G). e, Scatterplot shows cFC of all tested APA sites in REPAC analysis of CPSF6 knock-out primary CD4+ T cells in as compared to NT controls plotted against differential expression data as shown in in data aggregated from 3 biological replicates (donors E-G). 3’ UTRs are designated shortened (blue) for cFC < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. f, Immunoblot shows knock-out of CPSF5 in protein lysates harvested at day 4 post-editing in 3 biological replicates (donor Z-AB). g, HIV-1 infectivity (% GFP positive cells normalized to NT control per donor) at day 5 post-challenge with HIV-1 NL4-3 nef:IRES:GFP in indicated knock-out primary CD4+ T cells from 3 biological replicates (donors Z-AB) as assessed by flow cytometry. Each bar represents the average of technical triplicates + /- SD with individual data points shown. Statistics were calculated relative to the NT control per condition by one-way ANOVA with Dunnet’s test for multiple comparisons; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.

    Techniques Used: RNA Sequencing, Knock-Out, Control, Functional Assay, Comparison, Activation Assay, Quantitative Proteomics, Western Blot, Infection, Flow Cytometry

    Related Articles

    Cell-Signaling:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    Virus:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    Recombinant:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    Magnetic Beads:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    Reverse Transcription:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    Protease Inhibitor:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    Transfection:

    Article Title: Protein arginine methylation promotes global 3'UTR shortening in proliferating cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-SDMA Cell Signaling Technology Cat #13222; RRID: AB_2714013 Rabbit anti-ADMA Cell Signaling Technology Cat #13522; RRID: AB_2665370 Rabbit anti-NUDT21 Proteintech Cat #10322-1-AP; RRID: AB_2251496 Rabbit anti-CPSF73 Proteintech Cat #11609-1-AP; RRID: AB_2292188 Mouse anti-CSTF64 Proteintech Cat #68520-1-Ig; RRID: AB_3085229 Rabbit anti-PCF11 Proteintech Cat #84081-4-RR; RRID: AB_3671649 Rabbit anti-Symplekin Abcam Cat #ab251887; RRID: AB_3752789 Mouse anti-Actin Abcam Cat #ab8226; RRID: AB_306371 Mouse anti-GAPDH Proteintech Cat #60004-1-Ig; RRID: AB_2107436 Mouse Monoclonal ANTI-FLAG M2 Sigma-Aldrich Cat #F3165; RRID: AB_259529 Rabbit anti-ubiquitin Proteintech Cat# 10201-2-AP; RRID: AB_671515 Rabbit anti-c-Myc Cell Signaling Technology Cat# 18583; RRID: AB_2895543 Rabbit anti-FGF-2 Proteintech Cat #11234-1-AP; RRID: AB_2941976 Rabbit anti-ATG7 Cell Signaling Technology Cat #2631; RRID: AB_2227783 IRDye 680RD Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-68070; RRID: AB_10956588 IRDye 680RD Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-68071; RRID: AB_10956166 IRDye 800CW Goat anti-Mouse IgG (H+L) Li-Cor Cat #926-32210; RRID: AB_621842 IRDye 800CW Goat anti-Rabbit IgG (H+L) Li-Cor Cat #926-32211; RRID: AB_621843 Bacterial and virus strains NEB 5-alpha Competent E. coli New England Biolabs Cat #C2987H Chemicals, peptides, and recombinant proteins LLY-283 Sigma-Aldrich Cat# SML1972 GSK3368715 dihydrochloride Cambridge Bioscience Cat# HY-128717A-5mg OTS964 hydrochloride Cambridge Bioscience Cat# HY-12467-5mg Palbociclib Cambridge Bioscience Cat# HY-50767-5mg Anti-FLAG M2 Magnetic Beads Merck Cat# M8823-1ML 3X FLAG peptide Cambridge Bioscience Cat# HY-P0319-5MG Phusion High-Fidelity DNA Polymerase New England Biolabs Cat# M0530L SuperScript IV Reverse Transcriptase Invitrogen Cat# 18090200 DpnI New England Biolabs Cat# R0176L cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 11873580001 SUPERase ⋅ In RNase Inhibitor Invitrogen Cat# AM2694 DNase I NEB Cat# M0303L PhosSTOP Roche Cat# 4906845001 NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 12 well Invitrogen Cat# NP0322BOX NuPAGE Bis-Tris Mini Protein Gels, 4–12%, 15 well Invitrogen Cat# NP0323BOX NuPAGE Tris-Acetate Mini Protein Gels, 3-8%,12 well Invitrogen Cat# EA03752BOX Novex TBE Gels, 6%, 15 well Invitrogen Cat# EC62655BOX NuPAGE LDS sample buffer Invitrogen Cat# NP0007 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000008 Doxycycline hyclate Sigma-Aldrich Cat# D9891 Puromycin dihydrochloride Cambridge Bioscience Cat# T2219-10mg (Continued on next page) ll OPEN ACCESS Article e1 Molecular Cell 86, 1–17.e1–e9, November 19, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cycloheximide Cambridge Bioscience Cat# C001-5g MG-132 Sigma-Aldrich Cat# 474790-1MG Critical commercial assays Maxwell RSC simplyRNA Cells Kit Promega Cat# AS1390 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385612 CyQUANT NF Cell Proliferation Assay Kit Thermo Fisher Scientific Cat# C35007 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621L High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat# 4368814 DC Protein Concentration Assay BIORAD Cat# 5000116 Deposited data 3’ RNA-seq – 96hrs ADMAi vs SDMAi vs SDMAi+ADMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14928 3’ RNA-seq – DMAi timecourse in LU-99 This study EBI ArrayExpress: E-MTAB-14927 3’ RNA-seq – DMAi across panel of 10 cancer lines This study EBI ArrayExpress: E-MTAB-14932 3’ RNA-seq – DMAi in murine T cells This study EBI ArrayExpress: E-MTAB-14990 3’ RNA-seq – DMAi in patientderived organoids This study EBI ArrayExpress: E-MTAB-14992 3’ RNA-seq – siCFIM25 +/- DMAi in LU-99 This study EBI ArrayExpress: E-MTAB-14930 3’ RNA-seq – DMAi in RPE-1 and Palbociclib in LU-99 This study EBI ArrayExpress: E-MTAB-17463 3’ RNA-seq – DMAi +/- FLAG protein overexpression in LU-99 This study EBI ArrayExpress: E-MTAB-17496 Original Western blot images This study Mendeley Data: DOI:10.17632/m7b24gbyxw.1 Experimental models: Cell lines GP2D AstraZeneca Global Cell Bank N/A NCI-H838 AstraZeneca Global Cell Bank N/A LU-99 AstraZeneca Global Cell Bank N/A PANC0403 AstraZeneca Global Cell Bank N/A SK-MEL-2 AstraZeneca Global Cell Bank N/A SUM149PT AstraZeneca Global Cell Bank N/A U2OS AstraZeneca Global Cell Bank N/A HCT116 p53 +/+ Francis Crick Institute Cell Services N/A HCT116 p53 -/- Francis Crick Institute Cell Services N/A MCF-7 Francis Crick Institute Cell Services N/A hTERT-RPE1 Pines Lab N/A Organoid model - HUB-07-A2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - HUB-07-B2-051 Foundation Hubrecht Organoid Biobank N/A Organoid model - TEMPUS AZ-291290 Tempus Labs N/A Organoid model - TEMPUS AZ-574812 Tempus Labs N/A Experimental models: Organisms/strains Mice: C57BL/6J (wild type) University of York N/A Oligonucleotides Silencer Select Negative Control No. 1 siRNA Thermo Fisher Scientific Cat# 4390843 Silencer Select NUDT21 siRNA Thermo Fisher Scientific ID: s21772 Silencer Select ELAVL1 siRNA Thermo Fisher Scientific ID: s4608 Silencer Select KHDRBS1 siRNA Thermo Fisher Scientific ID: s20953 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–17.e1–e9, November 19, 2026 e2

    other:

    Article Title: Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.
    Article Snippet: Rabbit polyclonal anti-NUDT21 (CFIm25) (Proteintech Cat #10322-1-AP; RRID:AB_2251496), used at a 1:500 dilution.

    Article Title: SNW1 promotes lymphatic metastasis in bladder cancer by modulating SRPK1 splicing
    Article Snippet: NUDT21 , Proteintech , Cat# 10322-1-AP; RRID: AB_2251496.

    Western Blot:

    Article Title: Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.
    Article Snippet: RNA substrates were synthesized by runoff in vitro transcription using T7 RNA polymerase (New England Biolabs) and capped by vaccinia capping enzyme (New England Biolabs). .. RNA pulldown and western blotting experiments were performed as previously described38 using the following antibodies: mouse monoclonal anti-U170K clone 9C4.1 (Millipore, 05-1588; RRID: AB_10805959; 1:2,000), mouse monoclonal anti-U1A (Santa Cruz, sc-101149; RRID: AB_2193721; 1:2,000), rat monoclonal anti-U1C (Sigma, SAB4200188-200UL; RRID: AB_10640155; 1:2,000), rabbit polyclonal anti-CFIm68 (Bethyl, A301358A; RRID:AB_937785; 1:2,000), rabbit polyclonal anti-CFIm59 (Bethyl, A301-360A; RRID:AB_937864; 1:2,000), rabbit polyclonal anti-NUDT21 (CFIm25) (Proteintech, 10322-1-AP; RRID:AB_2251496; 1:500), rabbit polyclonal anti-NCBP2 (CBP20) (Bethyl, A302-553A; RRID: AB_2034872; 1:2,000), rabbit polyclonal anti-CPSF30 (Bethyl, A301-585A; RRID: AB_1078868; 1:2,000), goat anti-rabbit IgG HRP conjugate (Millipore, 12-348; RRID: AB_390191; 1:2,000), goat anti-mouse IgG HRP conjugate (Millipore, 12-349; RRID: AB_390192; 1:2,000) and rabbit anti-rat IgG HRP conjugate (Invitrogen, PA1-28573; RRID: AB_10980086; 1:2,000). .. Gapmer ASOs against AMOs were synthesized by Integrated DNA Technologies.

    Article Title: Transposable element–gene chimera cartography, origination and role in enhancing transcriptome plasticity
    Article Snippet: RNA substrates were synthesized by runoff in vitro transcription using T7 RNA polymerase (New England Biolabs) and capped by vaccinia capping enzyme (New England Biolabs). .. RNA pulldown and western blotting experiments were performed as previously described using the following antibodies: mouse monoclonal anti-U1-70K clone 9C4.1 (Millipore, 05-1588; RRID: AB_10805959; 1:2,000), mouse monoclonal anti-U1A (Santa Cruz, sc-101149; RRID: AB_2193721; 1:2,000), rat monoclonal anti-U1C (Sigma, SAB4200188-200UL; RRID: AB_10640155; 1:2,000), rabbit polyclonal anti-CFIm68 (Bethyl, A301-358A; RRID:AB_937785; 1:2,000), rabbit polyclonal anti-CFIm59 (Bethyl, A301-360A; RRID:AB_937864; 1:2,000), rabbit polyclonal anti-NUDT21 (CFIm25) (Proteintech, 10322-1-AP; RRID:AB_2251496; 1:500), rabbit polyclonal anti-NCBP2 (CBP20) (Bethyl, A302-553A; RRID: AB_2034872; 1:2,000), rabbit polyclonal anti-CPSF30 (Bethyl, A301-585A; RRID: AB_1078868; 1:2,000), goat anti-rabbit IgG HRP conjugate (Millipore, 12-348; RRID: AB_390191; 1:2,000), goat anti-mouse IgG HRP conjugate (Millipore, 12-349; RRID: AB_390192; 1:2,000) and rabbit anti-rat IgG HRP conjugate (Invitrogen, PA1-28573; RRID: AB_10980086; 1:2,000). .. Gapmer ASOs against AMOs were synthesized by Integrated DNA Technologies.



    Similar Products

    cpsd function  (MathWorks Inc)
    90
    MathWorks Inc cpsd function
    Cpsd Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/bio_rxiv__2025__06__07__658448-145-24-23
    Average 90 stars, based on 1 article reviews
    cpsd function - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    matlab functions cpsd  (MathWorks Inc)
    90
    MathWorks Inc matlab functions cpsd
    Matlab Functions Cpsd, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/bio_rxiv__2025__05__02__651511-84-26-24
    Average 90 stars, based on 1 article reviews
    matlab functions cpsd - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    cpsd function from  (MathWorks Inc)
    90
    MathWorks Inc cpsd function from
    Cpsd Function From, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/pm39426257-66-65-68
    Average 90 stars, based on 1 article reviews
    cpsd function from - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    cross power spectral density (cpsd) function  (MathWorks Inc)
    90
    MathWorks Inc cross power spectral density (cpsd) function
    Cross Power Spectral Density (Cpsd) Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/10__1108_slash_aeat___05___2023___0130-57-1-9
    Average 90 stars, based on 1 article reviews
    cross power spectral density (cpsd) function - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    cpsd function of  (MathWorks Inc)
    90
    MathWorks Inc cpsd function of
    Cpsd Function Of, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/pmc10955923__44172_2023_155_MOESM2_ESM-42-13-16
    Average 90 stars, based on 1 article reviews
    cpsd function of - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results