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cpsf5  (Proteintech)


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    Structured Review

    Proteintech cpsf5
    3’ UTRs are designated shortened (blue) for Compositional Fold Change (cFC) < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. b, Tracks show read coverage of TGFBR1 in RNA-Seq data from CPSF6 knock-out and NT control cells from one representative biological replicate (donor E), visualized using Integrative Genomics Visualizer. 3’ UTRs show evidence of shortening in response to CPSF6 knock-out. c, Charts show functional enrichment (Metascape) analysis of top pathways enriched in genes with shortened 3’ UTRs (left, blue) and lengthened 3’ UTRs (right, red) in response to CSPF6 knock-out in primary CD4+ T cells as assessed by REPAC analysis in 3 biological replicates (donors E-G). d, Violin plot shows comparison of cFC values from REPAC analysis in all tested genes as compared to genes in the indicated pathways (GO:0042110:T Cell activation, R-HSA-1280215: Cytokine Signaling in Immune system, R-HSA-913531: Interferon Signaling) in 3 biological replicates (donors E-G). e, Scatterplot shows cFC of all tested APA sites in REPAC analysis of CPSF6 knock-out primary CD4+ T cells in as compared to NT controls plotted against differential expression data as shown in in data aggregated from 3 biological replicates (donors E-G). 3’ UTRs are designated shortened (blue) for cFC < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. f, Immunoblot shows knock-out of <t>CPSF5</t> in protein lysates harvested at day 4 post-editing in 3 biological replicates (donor Z-AB). g, HIV-1 infectivity (% GFP positive cells normalized to NT control per donor) at day 5 post-challenge with HIV-1 NL4-3 nef:IRES:GFP in indicated knock-out primary CD4+ T cells from 3 biological replicates (donors Z-AB) as assessed by flow cytometry. Each bar represents the average of technical triplicates + /- SD with individual data points shown. Statistics were calculated relative to the NT control per condition by one-way ANOVA with Dunnet’s test for multiple comparisons; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.
    Cpsf5, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpsd+function+of/NUDT21+Antibody/pmc12714223-396-17-23
    Average 94 stars, based on 57 article reviews
    cpsf5 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells"

    Article Title: Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1013745

    3’ UTRs are designated shortened (blue) for Compositional Fold Change (cFC) < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. b, Tracks show read coverage of TGFBR1 in RNA-Seq data from CPSF6 knock-out and NT control cells from one representative biological replicate (donor E), visualized using Integrative Genomics Visualizer. 3’ UTRs show evidence of shortening in response to CPSF6 knock-out. c, Charts show functional enrichment (Metascape) analysis of top pathways enriched in genes with shortened 3’ UTRs (left, blue) and lengthened 3’ UTRs (right, red) in response to CSPF6 knock-out in primary CD4+ T cells as assessed by REPAC analysis in 3 biological replicates (donors E-G). d, Violin plot shows comparison of cFC values from REPAC analysis in all tested genes as compared to genes in the indicated pathways (GO:0042110:T Cell activation, R-HSA-1280215: Cytokine Signaling in Immune system, R-HSA-913531: Interferon Signaling) in 3 biological replicates (donors E-G). e, Scatterplot shows cFC of all tested APA sites in REPAC analysis of CPSF6 knock-out primary CD4+ T cells in as compared to NT controls plotted against differential expression data as shown in in data aggregated from 3 biological replicates (donors E-G). 3’ UTRs are designated shortened (blue) for cFC < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. f, Immunoblot shows knock-out of CPSF5 in protein lysates harvested at day 4 post-editing in 3 biological replicates (donor Z-AB). g, HIV-1 infectivity (% GFP positive cells normalized to NT control per donor) at day 5 post-challenge with HIV-1 NL4-3 nef:IRES:GFP in indicated knock-out primary CD4+ T cells from 3 biological replicates (donors Z-AB) as assessed by flow cytometry. Each bar represents the average of technical triplicates + /- SD with individual data points shown. Statistics were calculated relative to the NT control per condition by one-way ANOVA with Dunnet’s test for multiple comparisons; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.
    Figure Legend Snippet: 3’ UTRs are designated shortened (blue) for Compositional Fold Change (cFC) < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. b, Tracks show read coverage of TGFBR1 in RNA-Seq data from CPSF6 knock-out and NT control cells from one representative biological replicate (donor E), visualized using Integrative Genomics Visualizer. 3’ UTRs show evidence of shortening in response to CPSF6 knock-out. c, Charts show functional enrichment (Metascape) analysis of top pathways enriched in genes with shortened 3’ UTRs (left, blue) and lengthened 3’ UTRs (right, red) in response to CSPF6 knock-out in primary CD4+ T cells as assessed by REPAC analysis in 3 biological replicates (donors E-G). d, Violin plot shows comparison of cFC values from REPAC analysis in all tested genes as compared to genes in the indicated pathways (GO:0042110:T Cell activation, R-HSA-1280215: Cytokine Signaling in Immune system, R-HSA-913531: Interferon Signaling) in 3 biological replicates (donors E-G). e, Scatterplot shows cFC of all tested APA sites in REPAC analysis of CPSF6 knock-out primary CD4+ T cells in as compared to NT controls plotted against differential expression data as shown in in data aggregated from 3 biological replicates (donors E-G). 3’ UTRs are designated shortened (blue) for cFC < -0.25 and -log 10 (adjusted p-value) < 0.05 and lengthened (red) for cFC > 0.25 and -log 10 (adjusted p-value) < 0.05. Dots represent individual altered 3’ UTRs. f, Immunoblot shows knock-out of CPSF5 in protein lysates harvested at day 4 post-editing in 3 biological replicates (donor Z-AB). g, HIV-1 infectivity (% GFP positive cells normalized to NT control per donor) at day 5 post-challenge with HIV-1 NL4-3 nef:IRES:GFP in indicated knock-out primary CD4+ T cells from 3 biological replicates (donors Z-AB) as assessed by flow cytometry. Each bar represents the average of technical triplicates + /- SD with individual data points shown. Statistics were calculated relative to the NT control per condition by one-way ANOVA with Dunnet’s test for multiple comparisons; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001.

    Techniques Used: RNA Sequencing, Knock-Out, Control, Functional Assay, Comparison, Activation Assay, Quantitative Proteomics, Western Blot, Infection, Flow Cytometry

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    Western Blot:

    Article Title: Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.
    Article Snippet: RNA substrates were synthesized by runoff in vitro transcription using T7 RNA polymerase (New England Biolabs) and capped by vaccinia capping enzyme (New England Biolabs). .. RNA pulldown and western blotting experiments were performed as previously described38 using the following antibodies: mouse monoclonal anti-U170K clone 9C4.1 (Millipore, 05-1588; RRID: AB_10805959; 1:2,000), mouse monoclonal anti-U1A (Santa Cruz, sc-101149; RRID: AB_2193721; 1:2,000), rat monoclonal anti-U1C (Sigma, SAB4200188-200UL; RRID: AB_10640155; 1:2,000), rabbit polyclonal anti-CFIm68 (Bethyl, A301358A; RRID:AB_937785; 1:2,000), rabbit polyclonal anti-CFIm59 (Bethyl, A301-360A; RRID:AB_937864; 1:2,000), rabbit polyclonal anti-NUDT21 (CFIm25) (Proteintech, 10322-1-AP; RRID:AB_2251496; 1:500), rabbit polyclonal anti-NCBP2 (CBP20) (Bethyl, A302-553A; RRID: AB_2034872; 1:2,000), rabbit polyclonal anti-CPSF30 (Bethyl, A301-585A; RRID: AB_1078868; 1:2,000), goat anti-rabbit IgG HRP conjugate (Millipore, 12-348; RRID: AB_390191; 1:2,000), goat anti-mouse IgG HRP conjugate (Millipore, 12-349; RRID: AB_390192; 1:2,000) and rabbit anti-rat IgG HRP conjugate (Invitrogen, PA1-28573; RRID: AB_10980086; 1:2,000). .. Gapmer ASOs against AMOs were synthesized by Integrated DNA Technologies.

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    Recombinant:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SNW1 Bethyl Laboratories Cat# A300-785A SNW1 Proteintech Cat# 25926-1-AP; RRID:AB_2880298 NUDT21 Proteintech Cat# 10322-1-AP; RRID:AB_2251496 SRPK1 Proteintech Cat# 14073-1-AP; RRID:AB_2194745 CPSF6 Proteintech Cat# 15489-1-AP; RRID:AB_10694140 GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Normal Rabbit IgG Cell Signaling Technology Cat# 2729; RRID:AB_1031062 HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) Proteintech Cat# RGAR001; RRID:AB_3073505 Bacterial and virus strains hU6-MCS-CBh-gcGFP-IRES-puromycin GeneChem N/A Biological samples Bladder cancer tissues and adjacent normal tissues Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School N/A Chemicals, peptides, and recombinant proteins FBS Gibco Cat# A5256701 INTERFERin Polyplus Cat# 101000028 Lipomaster 2000 Vazyme Cat# TL201 D-Luciferin, Potassium Salt Vazyme Cat# DD1210 Puromycin Sangon Biotech Cat# A610593 Pierce TM Protease Inhibitor Thermo Scientific Cat# A32955 Trizol reagent Thermo Scientific Cat# 15596018 Matrigel Corning Cat# 354234 4% paraformaldehyde Biosharp Cat# BL539A Crystal Violet Staining Solution Beyotime Cat# C0121 Hematoxylin Servicebio Cat# G1004 Eosin Servicebio Cat# G1001 PureProteome Protein A/G magnetic beads Millipore Cat# LSKMAGAG10 PVDF membranes Bio-Rad Laboratories Cat# 1620177 SuperKine TM ECL Abbkine Cat# BMU102-CN Critical commercial assays HiScript III RT SuperMix Vazyme Cat# R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PureBinding®RNA Immunoprecipitation Kit GENESEED Cat# P0102 Deposited data Full-length transcriptome sequencing data This paper GSA-Human: HRA013690; https://ngdc. cncb.ac.cn/gsa-human/browse/ HRA013690 Experimental models: Cell lines T24 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu 55 UM-UC-3 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu217 (Continued on next page) e1 iScience 29, 114811, February 20, 2026 ..

    Virus:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
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    Protease Inhibitor:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
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    Staining:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SNW1 Bethyl Laboratories Cat# A300-785A SNW1 Proteintech Cat# 25926-1-AP; RRID:AB_2880298 NUDT21 Proteintech Cat# 10322-1-AP; RRID:AB_2251496 SRPK1 Proteintech Cat# 14073-1-AP; RRID:AB_2194745 CPSF6 Proteintech Cat# 15489-1-AP; RRID:AB_10694140 GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Normal Rabbit IgG Cell Signaling Technology Cat# 2729; RRID:AB_1031062 HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) Proteintech Cat# RGAR001; RRID:AB_3073505 Bacterial and virus strains hU6-MCS-CBh-gcGFP-IRES-puromycin GeneChem N/A Biological samples Bladder cancer tissues and adjacent normal tissues Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School N/A Chemicals, peptides, and recombinant proteins FBS Gibco Cat# A5256701 INTERFERin Polyplus Cat# 101000028 Lipomaster 2000 Vazyme Cat# TL201 D-Luciferin, Potassium Salt Vazyme Cat# DD1210 Puromycin Sangon Biotech Cat# A610593 Pierce TM Protease Inhibitor Thermo Scientific Cat# A32955 Trizol reagent Thermo Scientific Cat# 15596018 Matrigel Corning Cat# 354234 4% paraformaldehyde Biosharp Cat# BL539A Crystal Violet Staining Solution Beyotime Cat# C0121 Hematoxylin Servicebio Cat# G1004 Eosin Servicebio Cat# G1001 PureProteome Protein A/G magnetic beads Millipore Cat# LSKMAGAG10 PVDF membranes Bio-Rad Laboratories Cat# 1620177 SuperKine TM ECL Abbkine Cat# BMU102-CN Critical commercial assays HiScript III RT SuperMix Vazyme Cat# R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PureBinding®RNA Immunoprecipitation Kit GENESEED Cat# P0102 Deposited data Full-length transcriptome sequencing data This paper GSA-Human: HRA013690; https://ngdc. cncb.ac.cn/gsa-human/browse/ HRA013690 Experimental models: Cell lines T24 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu 55 UM-UC-3 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu217 (Continued on next page) e1 iScience 29, 114811, February 20, 2026 ..

    Magnetic Beads:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SNW1 Bethyl Laboratories Cat# A300-785A SNW1 Proteintech Cat# 25926-1-AP; RRID:AB_2880298 NUDT21 Proteintech Cat# 10322-1-AP; RRID:AB_2251496 SRPK1 Proteintech Cat# 14073-1-AP; RRID:AB_2194745 CPSF6 Proteintech Cat# 15489-1-AP; RRID:AB_10694140 GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Normal Rabbit IgG Cell Signaling Technology Cat# 2729; RRID:AB_1031062 HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) Proteintech Cat# RGAR001; RRID:AB_3073505 Bacterial and virus strains hU6-MCS-CBh-gcGFP-IRES-puromycin GeneChem N/A Biological samples Bladder cancer tissues and adjacent normal tissues Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School N/A Chemicals, peptides, and recombinant proteins FBS Gibco Cat# A5256701 INTERFERin Polyplus Cat# 101000028 Lipomaster 2000 Vazyme Cat# TL201 D-Luciferin, Potassium Salt Vazyme Cat# DD1210 Puromycin Sangon Biotech Cat# A610593 Pierce TM Protease Inhibitor Thermo Scientific Cat# A32955 Trizol reagent Thermo Scientific Cat# 15596018 Matrigel Corning Cat# 354234 4% paraformaldehyde Biosharp Cat# BL539A Crystal Violet Staining Solution Beyotime Cat# C0121 Hematoxylin Servicebio Cat# G1004 Eosin Servicebio Cat# G1001 PureProteome Protein A/G magnetic beads Millipore Cat# LSKMAGAG10 PVDF membranes Bio-Rad Laboratories Cat# 1620177 SuperKine TM ECL Abbkine Cat# BMU102-CN Critical commercial assays HiScript III RT SuperMix Vazyme Cat# R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PureBinding®RNA Immunoprecipitation Kit GENESEED Cat# P0102 Deposited data Full-length transcriptome sequencing data This paper GSA-Human: HRA013690; https://ngdc. cncb.ac.cn/gsa-human/browse/ HRA013690 Experimental models: Cell lines T24 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu 55 UM-UC-3 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu217 (Continued on next page) e1 iScience 29, 114811, February 20, 2026 ..

    Real-time Polymerase Chain Reaction:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SNW1 Bethyl Laboratories Cat# A300-785A SNW1 Proteintech Cat# 25926-1-AP; RRID:AB_2880298 NUDT21 Proteintech Cat# 10322-1-AP; RRID:AB_2251496 SRPK1 Proteintech Cat# 14073-1-AP; RRID:AB_2194745 CPSF6 Proteintech Cat# 15489-1-AP; RRID:AB_10694140 GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Normal Rabbit IgG Cell Signaling Technology Cat# 2729; RRID:AB_1031062 HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) Proteintech Cat# RGAR001; RRID:AB_3073505 Bacterial and virus strains hU6-MCS-CBh-gcGFP-IRES-puromycin GeneChem N/A Biological samples Bladder cancer tissues and adjacent normal tissues Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School N/A Chemicals, peptides, and recombinant proteins FBS Gibco Cat# A5256701 INTERFERin Polyplus Cat# 101000028 Lipomaster 2000 Vazyme Cat# TL201 D-Luciferin, Potassium Salt Vazyme Cat# DD1210 Puromycin Sangon Biotech Cat# A610593 Pierce TM Protease Inhibitor Thermo Scientific Cat# A32955 Trizol reagent Thermo Scientific Cat# 15596018 Matrigel Corning Cat# 354234 4% paraformaldehyde Biosharp Cat# BL539A Crystal Violet Staining Solution Beyotime Cat# C0121 Hematoxylin Servicebio Cat# G1004 Eosin Servicebio Cat# G1001 PureProteome Protein A/G magnetic beads Millipore Cat# LSKMAGAG10 PVDF membranes Bio-Rad Laboratories Cat# 1620177 SuperKine TM ECL Abbkine Cat# BMU102-CN Critical commercial assays HiScript III RT SuperMix Vazyme Cat# R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PureBinding®RNA Immunoprecipitation Kit GENESEED Cat# P0102 Deposited data Full-length transcriptome sequencing data This paper GSA-Human: HRA013690; https://ngdc. cncb.ac.cn/gsa-human/browse/ HRA013690 Experimental models: Cell lines T24 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu 55 UM-UC-3 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu217 (Continued on next page) e1 iScience 29, 114811, February 20, 2026 ..

    Immunoprecipitation:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SNW1 Bethyl Laboratories Cat# A300-785A SNW1 Proteintech Cat# 25926-1-AP; RRID:AB_2880298 NUDT21 Proteintech Cat# 10322-1-AP; RRID:AB_2251496 SRPK1 Proteintech Cat# 14073-1-AP; RRID:AB_2194745 CPSF6 Proteintech Cat# 15489-1-AP; RRID:AB_10694140 GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Normal Rabbit IgG Cell Signaling Technology Cat# 2729; RRID:AB_1031062 HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) Proteintech Cat# RGAR001; RRID:AB_3073505 Bacterial and virus strains hU6-MCS-CBh-gcGFP-IRES-puromycin GeneChem N/A Biological samples Bladder cancer tissues and adjacent normal tissues Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School N/A Chemicals, peptides, and recombinant proteins FBS Gibco Cat# A5256701 INTERFERin Polyplus Cat# 101000028 Lipomaster 2000 Vazyme Cat# TL201 D-Luciferin, Potassium Salt Vazyme Cat# DD1210 Puromycin Sangon Biotech Cat# A610593 Pierce TM Protease Inhibitor Thermo Scientific Cat# A32955 Trizol reagent Thermo Scientific Cat# 15596018 Matrigel Corning Cat# 354234 4% paraformaldehyde Biosharp Cat# BL539A Crystal Violet Staining Solution Beyotime Cat# C0121 Hematoxylin Servicebio Cat# G1004 Eosin Servicebio Cat# G1001 PureProteome Protein A/G magnetic beads Millipore Cat# LSKMAGAG10 PVDF membranes Bio-Rad Laboratories Cat# 1620177 SuperKine TM ECL Abbkine Cat# BMU102-CN Critical commercial assays HiScript III RT SuperMix Vazyme Cat# R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PureBinding®RNA Immunoprecipitation Kit GENESEED Cat# P0102 Deposited data Full-length transcriptome sequencing data This paper GSA-Human: HRA013690; https://ngdc. cncb.ac.cn/gsa-human/browse/ HRA013690 Experimental models: Cell lines T24 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu 55 UM-UC-3 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu217 (Continued on next page) e1 iScience 29, 114811, February 20, 2026 ..

    Sequencing:

    Article Title: SNW1 Promotes Lymphatic Metastasis in Bladder Cancer by Modulating SRPK1 Splicing
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SNW1 Bethyl Laboratories Cat# A300-785A SNW1 Proteintech Cat# 25926-1-AP; RRID:AB_2880298 NUDT21 Proteintech Cat# 10322-1-AP; RRID:AB_2251496 SRPK1 Proteintech Cat# 14073-1-AP; RRID:AB_2194745 CPSF6 Proteintech Cat# 15489-1-AP; RRID:AB_10694140 GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Normal Rabbit IgG Cell Signaling Technology Cat# 2729; RRID:AB_1031062 HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) Proteintech Cat# RGAR001; RRID:AB_3073505 Bacterial and virus strains hU6-MCS-CBh-gcGFP-IRES-puromycin GeneChem N/A Biological samples Bladder cancer tissues and adjacent normal tissues Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School N/A Chemicals, peptides, and recombinant proteins FBS Gibco Cat# A5256701 INTERFERin Polyplus Cat# 101000028 Lipomaster 2000 Vazyme Cat# TL201 D-Luciferin, Potassium Salt Vazyme Cat# DD1210 Puromycin Sangon Biotech Cat# A610593 Pierce TM Protease Inhibitor Thermo Scientific Cat# A32955 Trizol reagent Thermo Scientific Cat# 15596018 Matrigel Corning Cat# 354234 4% paraformaldehyde Biosharp Cat# BL539A Crystal Violet Staining Solution Beyotime Cat# C0121 Hematoxylin Servicebio Cat# G1004 Eosin Servicebio Cat# G1001 PureProteome Protein A/G magnetic beads Millipore Cat# LSKMAGAG10 PVDF membranes Bio-Rad Laboratories Cat# 1620177 SuperKine TM ECL Abbkine Cat# BMU102-CN Critical commercial assays HiScript III RT SuperMix Vazyme Cat# R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 PureBinding®RNA Immunoprecipitation Kit GENESEED Cat# P0102 Deposited data Full-length transcriptome sequencing data This paper GSA-Human: HRA013690; https://ngdc. cncb.ac.cn/gsa-human/browse/ HRA013690 Experimental models: Cell lines T24 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu 55 UM-UC-3 Cell Bank of Chinese Academy of Sciences, Shanghai Cat# TCHu217 (Continued on next page) e1 iScience 29, 114811, February 20, 2026 ..



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