Review



anti cox2 antibody  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc anti cox2 antibody
    Anti Cox2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/Cox2+XP+Rabbit+mAb/pmc13015246-2-0-3
    Average 97 stars, based on 1186 article reviews
    anti cox2 antibody - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: A potent icaritin derivative ameliorates Parkinson's disease via GPER-mediated mitophagy restoration and dopaminergic neuroprotection.
    Article Snippet: Icaritin (ICT), a major prenylated flavone from Epimedium with anti-osteoporosis, anti-cancer, and antiinflammatory properties, is limited clinically by poor water solubility and low bioavailability.. In this study, ICT derivatives were prepared via multi-step chemical reactions and five ICT derivatives (ICTA, ICTB, ICTC, ICTD and ICTE) were successfully synthesized.. Compared with ICT, most derivatives exhibited improved solubility and reduced cytotoxicity.

    other:

    Article Title: Comparative Effects of Dexamethasone and ASC Secretome in an Ex Vivo Osteoarthritis Co-Culture Model
    Article Snippet: Primary antibodies—rabbit anti-COX2 (#12282, Cell Signaling, Danvers, MA, USA; 1:1000 dilution), rabbit anti-IDO (#86630, Cell Signaling, Danvers, MA, USA; 1:1000 dilution), rabbit anti-COL2A1 (ab34712, Abcam, Cambridge, UK; 1:2000 dilution), and anti-GAPDH (sc-47724, Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:750 dilution)—were incubated overnight at 4 °C.

    Article Title: Bazedoxifene rescues hepatocytes from chemically induced oxidative ferroptotic injury in vivo and in vitro by inhibiting protein disulfide isomerase
    Article Snippet: Cox2 (D5H5) XP® Rabbit mAb , Cell Signaling Technology , Cat12282S; RRID: AB_2571729.

    Article Title: Serum metabolic fingerprinting for diagnosis and therapeutic applications of ovarian endometriosis
    Article Snippet: Anti-COX2 , Cell Signaling Technology , Cat# 12282, RRID: AB_2571729.

    Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors
    Article Snippet: Anti-COX2 antibody , Cell Signaling Technology , Cat# 12282; RRID: AB_2164118.

    DC Protein Assay:

    Article Title: Buckwheat Flavonoids Modulate Inflammation in RAW 264.7 Macrophages at Physiologically Relevant Concentrations via the LPS/COX-2 Pathway.
    Article Snippet: The cold RIPA buffer supplemented with phosphatase and protease inhibitors (Roche, Mannheim, Germany) was used to extract the cellular proteins from the samples obtained in the previous assay. .. Equal protein amounts (30 μg), quantified by the DC protein assay (Bio-Rad, Hercules, CA, USA), were loaded in a 10% SDSpolyacrylamide gel, transferred to nitrocellulose membranes (GE Healthcare, Buckinghamshire, UK), and incubated with primary COX-2 (74 kDa; D5H5, #12282) or β-actin (45 kDa; #4967) from Cell Signaling (MA, USA) at a dilution of 1:1000 and with secondary antirabbit antibody (#7074) at a dilution 1:5000. .. Band intensity quantification using ImageJ version 1.53k (NIH, USA) allowed the comparison between the treatments and the LPS-activated macrophages. β-Actin served as a loading control to normalize the COX-2 level.

    Incubation:

    Article Title: Buckwheat Flavonoids Modulate Inflammation in RAW 264.7 Macrophages at Physiologically Relevant Concentrations via the LPS/COX-2 Pathway.
    Article Snippet: The cold RIPA buffer supplemented with phosphatase and protease inhibitors (Roche, Mannheim, Germany) was used to extract the cellular proteins from the samples obtained in the previous assay. .. Equal protein amounts (30 μg), quantified by the DC protein assay (Bio-Rad, Hercules, CA, USA), were loaded in a 10% SDSpolyacrylamide gel, transferred to nitrocellulose membranes (GE Healthcare, Buckinghamshire, UK), and incubated with primary COX-2 (74 kDa; D5H5, #12282) or β-actin (45 kDa; #4967) from Cell Signaling (MA, USA) at a dilution of 1:1000 and with secondary antirabbit antibody (#7074) at a dilution 1:5000. .. Band intensity quantification using ImageJ version 1.53k (NIH, USA) allowed the comparison between the treatments and the LPS-activated macrophages. β-Actin served as a loading control to normalize the COX-2 level.

    Article Title: Bazedoxifene rescues hepatocytes from chemically induced oxidative ferroptotic injury in vivo and in vitro by inhibiting protein disulfide isomerase
    Article Snippet: .. For detection of GPX4 and COX-2, paraffin-embedded liver sections were incubated overnight at 4°C with either anti-GPX4 antibody (#HY- P80450 , 1:500; MedChemExpress, Monmouth Junction, NJ, USA) or anti-COX-2 antibody (#12282S, 1:1000; Cell Signaling Technology, Boston, MA, USA). .. After washing, sections were treated for 1 h with a rabbit polymer detection system (#PV-6001; ZSGB-BIO, Beijing, China) and visualized using a DAB staining kit (#ZLI-9017; ZSGB-BIO, Beijing, China).



    Similar Products

    95
    MedChemExpress zhang sen
    Zhang Sen, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/COX2+Antibody/10__1016_slash_j__apsb__2026__06__002-46-20-40
    Average 95 stars, based on 1 article reviews
    zhang sen - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Affinity Biosciences cox2
    Cox2, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/2+anti+cox/pm42320771-54-5-30
    Average 86 stars, based on 1 article reviews
    cox2 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Huabio Inc anti cyclooxygenase 2 cox2
    Anti Cyclooxygenase 2 Cox2, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/3+anti+caspase/pm42235724-79-9-12
    Average 86 stars, based on 1 article reviews
    anti cyclooxygenase 2 cox2 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Servicebio Inc recombinant anti cox2 cyclooxygenase 2 antibody
    Recombinant Anti Cox2 Cyclooxygenase 2 Antibody, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/anti+cox5a/pmc13198320-23-0-5
    Average 86 stars, based on 1 article reviews
    recombinant anti cox2 cyclooxygenase 2 antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Wanleibio rabbit anti cox2 antibody
    Rabbit Anti Cox2 Antibody, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/anti+cox2+rabbit/pm42132245-175-0-6
    Average 86 stars, based on 1 article reviews
    rabbit anti cox2 antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Wanleibio rabbit anti cox2 antibodies
    Rabbit Anti Cox2 Antibodies, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/anti+cox2+rabbit/pm42135568-244-0-6
    Average 86 stars, based on 1 article reviews
    rabbit anti cox2 antibodies - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Proteintech anti prostaglandin endoperoxide synthase 2
    PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and <t>PTGS2</t> in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.
    Anti Prostaglandin Endoperoxide Synthase 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/COX2%2F+Cyclooxygenase+2%2F+PTGS2+Antibody/pmc13040322-90-57-66
    Average 96 stars, based on 1 article reviews
    anti prostaglandin endoperoxide synthase 2 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Servicebio Inc gb114320 recombinant anti cox2 cyclooxygenase 2 antibody servicebio
    PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and <t>PTGS2</t> in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.
    Gb114320 Recombinant Anti Cox2 Cyclooxygenase 2 Antibody Servicebio, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/anti+cox5a/pm42030937-198-187-192
    Average 86 stars, based on 1 article reviews
    gb114320 recombinant anti cox2 cyclooxygenase 2 antibody servicebio - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc anti cox2 antibody
    PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and <t>PTGS2</t> in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.
    Anti Cox2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/Cox2+XP+Rabbit+mAb/pmc13015246-2-0-3
    Average 97 stars, based on 1 article reviews
    anti cox2 antibody - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

    Journal: Biomedical Reports

    Article Title: Peroxiredoxin 4 suppresses ferroptosis in esophageal squamous cell carcinoma by activating the phosphoinositide 3-kinase  signaling pathway

    doi: 10.3892/br.2026.2133

    Figure Lengend Snippet: PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

    Article Snippet: The primary antibodies were as follows: Anti-PRDX4 (1:2,000 dilution; cat. no. 10703-1-AP), anti-E-cadherin (1:20,000 dilution; cat. no. 20874-1-AP), anti-N-cadherin (1:2,000 dilution; cat. no. 22018-1-AP), anti-Vimentin (1:20,000 dilution; cat. no. 10366-1-AP), and anti-GPX4 (1:1,000 dilution; cat. no. 30388-1-AP; all from Proteintech Group, Inc.), anti-solute carrier family 7 member 11 (SLC7A11; 1:1,000 dilution; cat. no. DF12509; Affinity Biosciences, Ltd.), anti-prostaglandin-endoperoxide synthase 2 (PTGS2; 1:1,000 dilution; cat. no. 27308-1-AP; Proteintech Group, Inc.), anti-p-PI3K (1:1,000 dilution; cat. no. AP0427; ABclonal Biotech Co., Ltd.), anti-PI3K (1:1,000 dilution; cat. no. A19742; ABclonal Biotech Co., Ltd.), anti-p-AKT (1:2,000 dilution; cat. no. 28731-1-AP; Proteintech Group, Inc.), anti-AKT (1:2,000 dilution; cat. no. 10176-2-AP; Proteintech Group, Inc.) and anti-β-actin (1:2,000 dilution; cat. no. 20536-1-AP; Proteintech Group, Inc.).

    Techniques: Transfection, Western Blot, Control, Expressing, Small Interfering RNA