gene exp cox2 mm03294838 g1  (Thermo Fisher)

Journal: Redox Biology

Article Title: A novel hydrogen persulfide donor HPD1 alleviated LPS-induced neuroinflammation by modulating glial polarization through TLR4-MAPK/NF-κB pathway

doi: 10.1016/j.redox.2026.104430

Figure Lengend Snippet: Antibodies used for Western blotting.

Article Snippet: Primary , COX-2 , Rabbit , Mouse , 1:2000 , Proteintech (12375-1-AP).

Techniques: Western Blot

Journal: Redox Biology

Article Title: A novel hydrogen persulfide donor HPD1 alleviated LPS-induced neuroinflammation by modulating glial polarization through TLR4-MAPK/NF-κB pathway

doi: 10.1016/j.redox.2026.104430

Figure Lengend Snippet: HPD1 inhibited LPS-induced M1 polarization of BV2 microglia. (A-G) Relative mRNA expression levels of M1 pro-inflammatory markers, including (A) Il6 (encoding IL-6), (B) Il1b (IL-1β), (C) Tnf (TNFα), (D) Ptgs2 (COX-2), (E) Nos2 (iNOS), (F) Cd86 (CD86), and (G) Cxcl10 (CXCL10) (n = 4 or 5). (H) Representative Western blot images showing the protein expression levels of IL-6, pro-IL-1β, TNFα, COX-2, and iNOS. (I-M) Quantitative analysis of the protein expression levels of (I) IL-6, (J) pro-IL-1β, (K) TNFα, (L) COX-2, and (M) iNOS (n = 3 or 4). (N-P) Concentrations of (N) IL-6, (O) TNFα, and (P) NO in cell culture supernatants (n = 6 or 8). Data are presented as mean ± SEM and analyzed using one-way ANOVA followed by Tukey's multiple comparisons test. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Control group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. LPS-only group. CD86: cluster of differentiation 86; COX-2: cyclooxygenase-2; CXCL10: CXC motif chemokine ligand 10; iNOS: inducible nitric oxide synthase; IL-1β: interleukin-1β; IL-6: interleukin-6; LPS: lipopolysaccharide; NO: nitric oxide; TNFα: tumor necrosis factor-α.

Article Snippet: Primary , COX-2 , Rabbit , Mouse , 1:2000 , Proteintech (12375-1-AP).

Techniques: Expressing, Western Blot, Analysis, Cell Culture, Control

Journal: Exploration

Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage

doi: 10.1002/exp2.70233

Figure Lengend Snippet: Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of COX‐2, TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.

Article Snippet: Proteins in equivalent amounts were then separated on a 10% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to the PVDF membrane, followed by blocking in TBS‐T solution containing 5% skim milk for 1 h. Subsequently, the membrane was incubated with primary antibody against COX‐2 (66351‐1‐Ig, Proteintech), iNOS (80517‐1‐RR, Proteintech), and CD206 (60143‐1‐Ig, Proteintech) overnight at 4°C.

Techniques: Staining, Analysis, Immunofluorescence, Imaging, Immunohistochemical staining, Comparison