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MetaMorph Inc count nuclei function
Count Nuclei Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/count+nuclei+function/count+nuclei+function/pm19033387-329-4-2
Average 90 stars, based on 1 article reviews
count nuclei function - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: Serotonin Modulates Developmental Microglia via 5-HT2B Receptors: Potential Implication during Synaptic Refinement of Retinogeniculate Projections.
Article Snippet: For motility assays, the tested molecules were at the same concentration on both sides of the membrane, and microglia were incubated for 5 h. For chemotaxis assay, the tested molecules were placed exclusively in the lower compartment of the wells, and microglia were incubated for 2 h. In both cases, migration was stopped by fixation with 4% PFA in PB for 15 min. After nuclei staining with bis-benzamide (1:1,000 in PB), the inner (upper) part of the insert was cleaned with a cotton swab, the membrane was placed on a glass slide and mounted in Mowiol (Calbiochem; Sigma) and imaged using a Leica DM 6000B fluorescent microscope. .. The nuclei staining of cells that crossed the membrane was quantified, either manually (cell counting in randomly chosen regions of the membrane) and with a semi-automatized “count nuclei” function in MetaMorph, on series of 6 fields (objective 10x) per membrane (both approaches gave similar results). ..

Membrane:

Article Title: Serotonin Modulates Developmental Microglia via 5-HT2B Receptors: Potential Implication during Synaptic Refinement of Retinogeniculate Projections.
Article Snippet: For motility assays, the tested molecules were at the same concentration on both sides of the membrane, and microglia were incubated for 5 h. For chemotaxis assay, the tested molecules were placed exclusively in the lower compartment of the wells, and microglia were incubated for 2 h. In both cases, migration was stopped by fixation with 4% PFA in PB for 15 min. After nuclei staining with bis-benzamide (1:1,000 in PB), the inner (upper) part of the insert was cleaned with a cotton swab, the membrane was placed on a glass slide and mounted in Mowiol (Calbiochem; Sigma) and imaged using a Leica DM 6000B fluorescent microscope. .. The nuclei staining of cells that crossed the membrane was quantified, either manually (cell counting in randomly chosen regions of the membrane) and with a semi-automatized “count nuclei” function in MetaMorph, on series of 6 fields (objective 10x) per membrane (both approaches gave similar results). ..

Cell Counting:

Article Title: Serotonin Modulates Developmental Microglia via 5-HT2B Receptors: Potential Implication during Synaptic Refinement of Retinogeniculate Projections.
Article Snippet: For motility assays, the tested molecules were at the same concentration on both sides of the membrane, and microglia were incubated for 5 h. For chemotaxis assay, the tested molecules were placed exclusively in the lower compartment of the wells, and microglia were incubated for 2 h. In both cases, migration was stopped by fixation with 4% PFA in PB for 15 min. After nuclei staining with bis-benzamide (1:1,000 in PB), the inner (upper) part of the insert was cleaned with a cotton swab, the membrane was placed on a glass slide and mounted in Mowiol (Calbiochem; Sigma) and imaged using a Leica DM 6000B fluorescent microscope. .. The nuclei staining of cells that crossed the membrane was quantified, either manually (cell counting in randomly chosen regions of the membrane) and with a semi-automatized “count nuclei” function in MetaMorph, on series of 6 fields (objective 10x) per membrane (both approaches gave similar results). ..

other:

Article Title: The adaptor complex AP-2 regulates post-endocytic trafficking through the non-clathrin Arf6-dependent endocytic pathway.
Article Snippet: Using the Metamorph ‘Count Nuclei’ function, β1-integrin- and CD63-positive spots were determined based on predetermined threshold intensity as well as diameter.

Article Title: Discriminative detection and enumeration of microbial life in marine subsurface sediments.
Article Snippet: For computer counting without image analysis, all images were smoothed using a median filter (3 3 pixel square), and then fluorescent signals were counted with the ‘Count Nuclei’ function of the MetaMorph software.

Article Title: Resident CAPS on dense-core vesicles docks and primes vesicles for fusion
Article Snippet: Individual vesicles were counted either using “Count Nuclei” function in Metamorph (method 1) or “Find Maxima” function in FIJI (method 2).



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