Journal: Journal of Nutrition and Metabolism
Article Title: Maternal High‐Fat Diet, During Preimplantation or Gestation/Lactation, Alters Mouse Offspring Metabolic, Molecular and Locomotor Phenotypes
doi: 10.1155/jnme/5286440
Figure Lengend Snippet: Maternal high‐fat diet affects motor coordination in female and male adult offspring. Inverted screen test latency to fall in the female (A) and male (B) offsprings at 6 weeks old. Number of female offspring: 21 CFD, 23 EmbHFD, 24 HFD, number of male offspring: 20 CFD, 24 EmbHFD, 24 HFD, number of mothers: 6 CFD, 8 EmbHFD and 8 HFD. Rotarod latency to fall for female (C) and male (D) offsprings at 7 and 12 weeks old. Number of female offspring: 26 CFD, 24 EmbHFD, 26 HFD, number of male offspring: 26 CFD, 24 EmbHFD, 26 HFD, number of mothers: 9 CFD, 8 EmbHFD and 9 HFD. A 10‐min open field test of 4‐week‐old offspring was analysed as two periods of 5 min in females (E‐G‐I‐K) and males (F‐H‐J‐L). Distance travelled (E, F), resting time (G, H), vertical time (I, J) and jump counts (K, L) were obtained with the ENV250 software. Number of female offspring: 25 CFD, 23 EmbHFD, 27 HFD, number of male offspring: 26 CFD, 24 EmbHFD, 26 HFD, number of mothers: 9 CFD, 8 EmbHFD and 9 HFD. Representative pictures of Sudan black B–stained sections of mouse offspring gastrocnemius muscle at 15 weeks (M). Cells that showed darker blue are considered as positive (higher lipid content, black arrow) compared to a normal cell (red arrow). Scale bar represents 200 μm. Percentage of Sudan Black B–positive cells (SBB+) in female (N) and male (O) offspring gastrocnemius muscle at 15 weeks. Number of female offspring: 9 CFD, 8 EmbHFD, 9 HFD, number of male offspring: 9 CFD, 8 EmbHFD, 9 HFD, number of mothers: 9 CFD, 8 EmbHFD and 9 HFD. CFD data (black circles), EmbHFD data (green squares) and HFD data (red triangles) were analysed by multilevel random effects regression and shown as dot plots with mean. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 between diet groups or between ages.
Article Snippet: Animal tracking was performed using the ENV250 Test Software (MED Associates, Inc. Vermont) and sessions were video‐recorded.
Techniques: Software, Staining