control empty pfn2k vector (OriGene)
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OriGene
control empty pfn2k vector
Control Empty Pfn2k Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+vectors/Negative+shRNA+control+in+pGFP-V-RS+Vector/pmc02709342-239-22-25
Average 91 stars, based on 36 article reviews
Control Empty Pfn2k Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+vectors/Negative+shRNA+control+in+pGFP-V-RS+Vector/pmc02709342-239-22-25
Average 91 stars, based on 36 article reviews
control empty pfn2k vector - by Bioz Stars,
2026-10
91/100 stars
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Knockdown:Article Title: Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer Article Snippet: To generate UGDH overexpression lines, LNCaP AD and LNCaP CR cells were transduced with lentiviral particles packaged to contain plasmids encoding wild type UGDH with a C-terminal Flag epitope tag, or a vector control expressing EGFP (UGDH EX-Q0483-Lv102 and EX-EGFP-Lv105, respectively, from GeneCopoeia, Rockville, MD, USA). .. To achieve Article Title: Endocannabinoid signalling in stem cells and cerebral organoids drives differentiation to deep layer projection neurons via CB 1 receptors. Article Snippet: .. CB1 receptor knockdown was performed in R1 mES cells with 10 μg Transfection:Article Title: Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer Article Snippet: To generate UGDH overexpression lines, LNCaP AD and LNCaP CR cells were transduced with lentiviral particles packaged to contain plasmids encoding wild type UGDH with a C-terminal Flag epitope tag, or a vector control expressing EGFP (UGDH EX-Q0483-Lv102 and EX-EGFP-Lv105, respectively, from GeneCopoeia, Rockville, MD, USA). .. To achieve Article Title: Pseudolaric Acid B Targets CD147 to Selectively Kill Acute Myeloid Leukemia Cells Article Snippet: .. CD147 gene-specific short hairpin RNA (shRNA-CD147-A and shRNA-CD147-B; OriGene; Rockville, MD, USA; see for sequences) and a scrambled Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. Stable shRNA-mediated PAK6 transfection For stable clone generation, L02 cells with FAT4-KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Article Title: The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis Article Snippet: For transfections, cells were incubated with CPNE7 shRNA (ORIGENE, Rockville, MD, USA, TL305242) at 50 μmol/L concentrations in Opti MEM medium. .. The transfection efficiencies of cell lines were tested by transfection of the Article Title: Glia Maturation Factor-Gamma Regulates Autophagy and Scavenger Receptor-Mediated Phagocytosis in Murine Macrophages Article Snippet: For GMFγ + Nrf2 double-knockdown, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with an siRNA against Nrf2 (SR427248; ORIGENE), followed by transfection with an siRNA against GMFg for another 48 h, using Nucleofector II. .. For GMFγ overexpression studies, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with a GMFgGFP (ORIGENE) or shRNA:Article Title: Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer Article Snippet: To generate UGDH overexpression lines, LNCaP AD and LNCaP CR cells were transduced with lentiviral particles packaged to contain plasmids encoding wild type UGDH with a C-terminal Flag epitope tag, or a vector control expressing EGFP (UGDH EX-Q0483-Lv102 and EX-EGFP-Lv105, respectively, from GeneCopoeia, Rockville, MD, USA). .. To achieve Article Title: Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V Article Snippet: Ba/F3 cells were plated onto 3.5 cm dishes coated with fibronectin fragments (Retronectin dish, Takara, Shiga, Japan) and cultured with 1 ml virus supernatant for 72 h. Retrovirus-infected Ba/F3 cells were isolated as GFP-positive cells by FACS Aria (BD Biosciences, Franklin Lakes, NJ). .. Expression vectors for CALM-specific shRNA and SCR shRNA (a negative control) were generated by cloning these sequences into Article Title: Pseudolaric Acid B Targets CD147 to Selectively Kill Acute Myeloid Leukemia Cells Article Snippet: .. CD147 gene-specific short hairpin RNA (shRNA-CD147-A and shRNA-CD147-B; OriGene; Rockville, MD, USA; see for sequences) and a scrambled Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. Stable shRNA-mediated PAK6 transfection For stable clone generation, L02 cells with FAT4-KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Article Title: The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis Article Snippet: For transfections, cells were incubated with CPNE7 shRNA (ORIGENE, Rockville, MD, USA, TL305242) at 50 μmol/L concentrations in Opti MEM medium. .. The transfection efficiencies of cell lines were tested by transfection of the Control:Article Title: Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer Article Snippet: To generate UGDH overexpression lines, LNCaP AD and LNCaP CR cells were transduced with lentiviral particles packaged to contain plasmids encoding wild type UGDH with a C-terminal Flag epitope tag, or a vector control expressing EGFP (UGDH EX-Q0483-Lv102 and EX-EGFP-Lv105, respectively, from GeneCopoeia, Rockville, MD, USA). .. To achieve Article Title: Long non-coding RNA H19 promotes the proliferation, migration and invasion while inhibits apoptosis of hypertrophic scarring fibroblasts by targeting miR-3187-3p/GAB1 axis. Article Snippet: Background: It had been reported that long non-coding RNA (lncRNA) H19 was associated with the proliferation of fibroblasts.. However, the regulatory mechanism of H19 remains unclear.. Thus, the study was designed to explore the underlying mechanism of H19 in the process of Hypertrophic scarring (HS). Article Title: Glia Maturation Factor-Gamma Regulates Autophagy and Scavenger Receptor-Mediated Phagocytosis in Murine Macrophages Article Snippet: For GMFγ + Nrf2 double-knockdown, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with an siRNA against Nrf2 (SR427248; ORIGENE), followed by transfection with an siRNA against GMFg for another 48 h, using Nucleofector II. .. For GMFγ overexpression studies, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with a GMFgGFP (ORIGENE) or Expressing:Article Title: Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V Article Snippet: Ba/F3 cells were plated onto 3.5 cm dishes coated with fibronectin fragments (Retronectin dish, Takara, Shiga, Japan) and cultured with 1 ml virus supernatant for 72 h. Retrovirus-infected Ba/F3 cells were isolated as GFP-positive cells by FACS Aria (BD Biosciences, Franklin Lakes, NJ). .. Expression vectors for CALM-specific shRNA and SCR shRNA (a negative control) were generated by cloning these sequences into Negative Control:Article Title: Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V Article Snippet: Ba/F3 cells were plated onto 3.5 cm dishes coated with fibronectin fragments (Retronectin dish, Takara, Shiga, Japan) and cultured with 1 ml virus supernatant for 72 h. Retrovirus-infected Ba/F3 cells were isolated as GFP-positive cells by FACS Aria (BD Biosciences, Franklin Lakes, NJ). .. Expression vectors for CALM-specific shRNA and SCR shRNA (a negative control) were generated by cloning these sequences into Article Title: Pseudolaric Acid B Targets CD147 to Selectively Kill Acute Myeloid Leukemia Cells Article Snippet: .. CD147 gene-specific short hairpin RNA (shRNA-CD147-A and shRNA-CD147-B; OriGene; Rockville, MD, USA; see for sequences) and a scrambled Article Title: Long non-coding RNA H19 promotes the proliferation, migration and invasion while inhibits apoptosis of hypertrophic scarring fibroblasts by targeting miR-3187-3p/GAB1 axis. Article Snippet: Background: It had been reported that long non-coding RNA (lncRNA) H19 was associated with the proliferation of fibroblasts.. However, the regulatory mechanism of H19 remains unclear.. Thus, the study was designed to explore the underlying mechanism of H19 in the process of Hypertrophic scarring (HS). Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. Stable shRNA-mediated PAK6 transfection For stable clone generation, L02 cells with FAT4-KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Article Title: The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis Article Snippet: For transfections, cells were incubated with CPNE7 shRNA (ORIGENE, Rockville, MD, USA, TL305242) at 50 μmol/L concentrations in Opti MEM medium. .. The transfection efficiencies of cell lines were tested by transfection of the Generated:Article Title: Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V Article Snippet: Ba/F3 cells were plated onto 3.5 cm dishes coated with fibronectin fragments (Retronectin dish, Takara, Shiga, Japan) and cultured with 1 ml virus supernatant for 72 h. Retrovirus-infected Ba/F3 cells were isolated as GFP-positive cells by FACS Aria (BD Biosciences, Franklin Lakes, NJ). .. Expression vectors for CALM-specific shRNA and SCR shRNA (a negative control) were generated by cloning these sequences into Cloning:Article Title: Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V Article Snippet: Ba/F3 cells were plated onto 3.5 cm dishes coated with fibronectin fragments (Retronectin dish, Takara, Shiga, Japan) and cultured with 1 ml virus supernatant for 72 h. Retrovirus-infected Ba/F3 cells were isolated as GFP-positive cells by FACS Aria (BD Biosciences, Franklin Lakes, NJ). .. Expression vectors for CALM-specific shRNA and SCR shRNA (a negative control) were generated by cloning these sequences into Cell Culture:Article Title: Pseudolaric Acid B Targets CD147 to Selectively Kill Acute Myeloid Leukemia Cells Article Snippet: .. CD147 gene-specific short hairpin RNA (shRNA-CD147-A and shRNA-CD147-B; OriGene; Rockville, MD, USA; see for sequences) and a scrambled Small Interfering RNA:Article Title: Long non-coding RNA H19 promotes the proliferation, migration and invasion while inhibits apoptosis of hypertrophic scarring fibroblasts by targeting miR-3187-3p/GAB1 axis. Article Snippet: Background: It had been reported that long non-coding RNA (lncRNA) H19 was associated with the proliferation of fibroblasts.. However, the regulatory mechanism of H19 remains unclear.. Thus, the study was designed to explore the underlying mechanism of H19 in the process of Hypertrophic scarring (HS). Stable Transfection:Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. Stable shRNA-mediated PAK6 transfection For stable clone generation, L02 cells with FAT4-KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Plasmid Preparation:Article Title: The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis Article Snippet: For transfections, cells were incubated with CPNE7 shRNA (ORIGENE, Rockville, MD, USA, TL305242) at 50 μmol/L concentrations in Opti MEM medium. .. The transfection efficiencies of cell lines were tested by transfection of the Article Title: Glia Maturation Factor-Gamma Regulates Autophagy and Scavenger Receptor-Mediated Phagocytosis in Murine Macrophages Article Snippet: For GMFγ + Nrf2 double-knockdown, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with an siRNA against Nrf2 (SR427248; ORIGENE), followed by transfection with an siRNA against GMFg for another 48 h, using Nucleofector II. .. For GMFγ overexpression studies, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with a GMFgGFP (ORIGENE) or Over Expression:Article Title: Glia Maturation Factor-Gamma Regulates Autophagy and Scavenger Receptor-Mediated Phagocytosis in Murine Macrophages Article Snippet: For GMFγ + Nrf2 double-knockdown, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with an siRNA against Nrf2 (SR427248; ORIGENE), followed by transfection with an siRNA against GMFg for another 48 h, using Nucleofector II. .. For GMFγ overexpression studies, RAW264.7 cells (2 × 106 cells) were transiently transfected for 24 h with a GMFgGFP (ORIGENE) or |