control plasmid encoding gpf shrna (OriGene)
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Control Plasmid Encoding Gpf Shrna, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+vector/Negative+shRNA+control+in+pRS+Vector/us11008573-811-20-10
Average 94 stars, based on 73 article reviews
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shRNA:Article Title: Sertad1 exacerbates cardiomyocytes ferroptosis in sepsis-induced myocardial injury by inhibiting HIPK2. Article Snippet: factor-alpha (TNF-α), culminating in multiple organ dysfunction [1–3].. Myocardial injury, a frequent complication of sepsis, arises from multifactorial mechanisms including mitochondrial dysfunction, microcirculatory disturbances, calcium dysregulation, and ferroptosis activation [4].. Clinically, sepsis-induced myocardial injury manifests as elevated cardiac troponin levels, ventricular diastolic dysfunction, and reduced cardiac output—distinct from ischemic injury by typically maintaining patent coronary arteries [5, 6]. Article Title: ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis Article Snippet: Following 48 h of transfection, the cells were treated with 100 μg/mL of G418 (Sigma‐Aldrich) every 2 days for 10 days total to establish a stable cell line. .. To ensure the stable knockdown of ISG15 in the POCC cells, TR127 cells were transfected with pRS vector containing four Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness Article Snippet: Cytotoxicity was assessed using the CyQUANTTM LDH Cytotoxicity Assay (Invitrogen). .. Lentiviral knockdown was performed using gene-specific human shRNA lentiviral particles (GPR18: Locus ID 2841) or Article Title: Baicalin Inhibits VSMC Phenotypic Transition and Ameliorates Atherosclerosis via GSK3β-TCF21 Signaling Pathway Article Snippet: .. TCF21 lentiviral vectors shRNA and Article Title: SLC25A33-mediated mitochondrial DNA synthesis plays a critical role in the inflammatory response of M1 macrophages by contributing to mitochondrial ROS and VDAC oligomerization Article Snippet: Cells were transfected with scramble siRNA, siSLC25A33, siMyD88, siPI3K, simTOR, siTAK1, siTBK1, siATF4 (Bioneer), SLC25A33 ORF Clone (Origene) or ATF4 ORF Clone (GeneCopoeia) using Lipofectamine TM 3000 (Thermo Fisher Scientific). .. Lentiviral plasmid vectors shSLC25A33, shATF4, and Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. For stable clone generation, L02 cells with FAT4 -KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Article Title: Amylin inhibits gastric cancer progression by targeting CCN1 and affecting the PI3K/AKT signalling pathway. Article Snippet: .. As controls, either pCMV6 without an insert or pRS with negative Control:Article Title: Sertad1 exacerbates cardiomyocytes ferroptosis in sepsis-induced myocardial injury by inhibiting HIPK2. Article Snippet: factor-alpha (TNF-α), culminating in multiple organ dysfunction [1–3].. Myocardial injury, a frequent complication of sepsis, arises from multifactorial mechanisms including mitochondrial dysfunction, microcirculatory disturbances, calcium dysregulation, and ferroptosis activation [4].. Clinically, sepsis-induced myocardial injury manifests as elevated cardiac troponin levels, ventricular diastolic dysfunction, and reduced cardiac output—distinct from ischemic injury by typically maintaining patent coronary arteries [5, 6]. Article Title: ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis Article Snippet: Following 48 h of transfection, the cells were treated with 100 μg/mL of G418 (Sigma‐Aldrich) every 2 days for 10 days total to establish a stable cell line. .. To ensure the stable knockdown of ISG15 in the POCC cells, TR127 cells were transfected with pRS vector containing four Article Title: Egfl6 promotes ovarian cancer progression by enhancing the immunosuppressive functions of tumor-associated myeloid cells Article Snippet: Dosing was selected based on prior studies, pharmacokinetic studies, and toxicity studies (performed by Jackson laboratories) ( , ). .. 2F8c cells were transfected with Egfl6-pCMV6-Entry vector or Article Title: Baicalin Inhibits VSMC Phenotypic Transition and Ameliorates Atherosclerosis via GSK3β-TCF21 Signaling Pathway Article Snippet: .. TCF21 lentiviral vectors shRNA and Article Title: SLC25A33-mediated mitochondrial DNA synthesis plays a critical role in the inflammatory response of M1 macrophages by contributing to mitochondrial ROS and VDAC oligomerization Article Snippet: Cells were transfected with scramble siRNA, siSLC25A33, siMyD88, siPI3K, simTOR, siTAK1, siTBK1, siATF4 (Bioneer), SLC25A33 ORF Clone (Origene) or ATF4 ORF Clone (GeneCopoeia) using Lipofectamine TM 3000 (Thermo Fisher Scientific). .. Lentiviral plasmid vectors shSLC25A33, shATF4, and Bioprocessing:Article Title: Sertad1 exacerbates cardiomyocytes ferroptosis in sepsis-induced myocardial injury by inhibiting HIPK2. Article Snippet: factor-alpha (TNF-α), culminating in multiple organ dysfunction [1–3].. Myocardial injury, a frequent complication of sepsis, arises from multifactorial mechanisms including mitochondrial dysfunction, microcirculatory disturbances, calcium dysregulation, and ferroptosis activation [4].. Clinically, sepsis-induced myocardial injury manifests as elevated cardiac troponin levels, ventricular diastolic dysfunction, and reduced cardiac output—distinct from ischemic injury by typically maintaining patent coronary arteries [5, 6]. Knockdown:Article Title: ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis Article Snippet: Following 48 h of transfection, the cells were treated with 100 μg/mL of G418 (Sigma‐Aldrich) every 2 days for 10 days total to establish a stable cell line. .. To ensure the stable knockdown of ISG15 in the POCC cells, TR127 cells were transfected with pRS vector containing four Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness Article Snippet: Cytotoxicity was assessed using the CyQUANTTM LDH Cytotoxicity Assay (Invitrogen). .. Lentiviral knockdown was performed using gene-specific human shRNA lentiviral particles (GPR18: Locus ID 2841) or Transfection:Article Title: ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis Article Snippet: Following 48 h of transfection, the cells were treated with 100 μg/mL of G418 (Sigma‐Aldrich) every 2 days for 10 days total to establish a stable cell line. .. To ensure the stable knockdown of ISG15 in the POCC cells, TR127 cells were transfected with pRS vector containing four Article Title: Egfl6 promotes ovarian cancer progression by enhancing the immunosuppressive functions of tumor-associated myeloid cells Article Snippet: Dosing was selected based on prior studies, pharmacokinetic studies, and toxicity studies (performed by Jackson laboratories) ( , ). .. 2F8c cells were transfected with Egfl6-pCMV6-Entry vector or Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. For stable clone generation, L02 cells with FAT4 -KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Plasmid Preparation:Article Title: ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis Article Snippet: Following 48 h of transfection, the cells were treated with 100 μg/mL of G418 (Sigma‐Aldrich) every 2 days for 10 days total to establish a stable cell line. .. To ensure the stable knockdown of ISG15 in the POCC cells, TR127 cells were transfected with pRS vector containing four Article Title: SLC25A33-mediated mitochondrial DNA synthesis plays a critical role in the inflammatory response of M1 macrophages by contributing to mitochondrial ROS and VDAC oligomerization Article Snippet: Cells were transfected with scramble siRNA, siSLC25A33, siMyD88, siPI3K, simTOR, siTAK1, siTBK1, siATF4 (Bioneer), SLC25A33 ORF Clone (Origene) or ATF4 ORF Clone (GeneCopoeia) using Lipofectamine TM 3000 (Thermo Fisher Scientific). .. Lentiviral plasmid vectors shSLC25A33, shATF4, and Construct:Article Title: ISG15 mediates the function of extracellular vesicles in promoting ovarian cancer progression and metastasis Article Snippet: Following 48 h of transfection, the cells were treated with 100 μg/mL of G418 (Sigma‐Aldrich) every 2 days for 10 days total to establish a stable cell line. .. To ensure the stable knockdown of ISG15 in the POCC cells, TR127 cells were transfected with pRS vector containing four Negative Control:Article Title: N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4 + T cell responsiveness Article Snippet: Cytotoxicity was assessed using the CyQUANTTM LDH Cytotoxicity Assay (Invitrogen). .. Lentiviral knockdown was performed using gene-specific human shRNA lentiviral particles (GPR18: Locus ID 2841) or Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. For stable clone generation, L02 cells with FAT4 -KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or Article Title: Amylin inhibits gastric cancer progression by targeting CCN1 and affecting the PI3K/AKT signalling pathway. Article Snippet: .. As controls, either pCMV6 without an insert or pRS with negative Stable Transfection:Article Title: FAT4 loss initiates hepatocarcinogenesis through the switching of canonical to noncanonical WNT signaling pathways Article Snippet: .. For stable clone generation, L02 cells with FAT4 -KO were transfected with 2 μg of p21 (RAC1)-activated kinase 6 (PAK6)–specific shRNA or |
