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control shrna shctrl  (OriGene)


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    Structured Review

    OriGene control shrna shctrl
    Figure 3. Effect of RASSF10 on cell growth and cell migration/invasion. (a) Overexpression of RASSF10 in AGS and MKN45 cells after stable transfection with RASSF10 expression vectors was confirmed by western blotting. (b) MTS viability assays showed RASSF10 expression significantly suppressed growth of AGS and MKN45 cells. (c) Colony-formation ability of AGS and MKN45 cells was significantly inhibited by RASSF10 expression. (d1) RASSF10 expression was knocked down in GES-1 cells by transfection of <t>shRNA</t> against RASSF10. (d2) Cell growth of GES-1 was significantly increased after knockdown of RASSF10. (e) Cell migration ability was significantly inhibited by RASSF10 expression in AGS cells as indicated by wound-healing assay. (f) Cell invasiveness was significantly reduced by RASSF10 expression in AGS and MKN45 cells as indicated by matrigel invasion assay.
    Control Shrna Shctrl, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+vector/Scrambled+shRNA+control+in+pGFP-V-RS+shRNA+Vector/pm26279301-240-17-23
    Average 95 stars, based on 152 article reviews
    control shrna shctrl - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Ras association domain family member 10 suppresses gastric cancer growth by cooperating with GSTP1 to regulate JNK/c-Jun/AP-1 pathway."

    Article Title: Ras association domain family member 10 suppresses gastric cancer growth by cooperating with GSTP1 to regulate JNK/c-Jun/AP-1 pathway.

    Journal: Oncogene

    doi: 10.1038/onc.2015.300

    Figure 3. Effect of RASSF10 on cell growth and cell migration/invasion. (a) Overexpression of RASSF10 in AGS and MKN45 cells after stable transfection with RASSF10 expression vectors was confirmed by western blotting. (b) MTS viability assays showed RASSF10 expression significantly suppressed growth of AGS and MKN45 cells. (c) Colony-formation ability of AGS and MKN45 cells was significantly inhibited by RASSF10 expression. (d1) RASSF10 expression was knocked down in GES-1 cells by transfection of shRNA against RASSF10. (d2) Cell growth of GES-1 was significantly increased after knockdown of RASSF10. (e) Cell migration ability was significantly inhibited by RASSF10 expression in AGS cells as indicated by wound-healing assay. (f) Cell invasiveness was significantly reduced by RASSF10 expression in AGS and MKN45 cells as indicated by matrigel invasion assay.
    Figure Legend Snippet: Figure 3. Effect of RASSF10 on cell growth and cell migration/invasion. (a) Overexpression of RASSF10 in AGS and MKN45 cells after stable transfection with RASSF10 expression vectors was confirmed by western blotting. (b) MTS viability assays showed RASSF10 expression significantly suppressed growth of AGS and MKN45 cells. (c) Colony-formation ability of AGS and MKN45 cells was significantly inhibited by RASSF10 expression. (d1) RASSF10 expression was knocked down in GES-1 cells by transfection of shRNA against RASSF10. (d2) Cell growth of GES-1 was significantly increased after knockdown of RASSF10. (e) Cell migration ability was significantly inhibited by RASSF10 expression in AGS cells as indicated by wound-healing assay. (f) Cell invasiveness was significantly reduced by RASSF10 expression in AGS and MKN45 cells as indicated by matrigel invasion assay.

    Techniques Used: Migration, Over Expression, Stable Transfection, Expressing, Western Blot, Transfection, shRNA, Knockdown, Wound Healing Assay, Invasion Assay

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    Article Title: S1P-S1PR1 signaling impairs CD8 + T cell metabolism and effector function in tumors
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    Expressing:

    Article Title: S1P-S1PR1 signaling impairs CD8 + T cell metabolism and effector function in tumors
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    Plasmid Preparation:

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    Control:

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    Article Title: Peroxisome Proliferator-Activated Receptor Family of Lipid-Activated Nuclear Receptors Alpha Silencing Promotes Oxidative Stress and Hypertrophic Phenotype in Rat Cardiac Cells
    Article Snippet: In detail, 250 ng of plasmids were transfected using Lipofectamine 2000 (Thermo Fischer Scientific, Waltham, MA, USA) following the manufacturer’s instructions. .. The following shRNA plasmids were used: 29-mer scrambled shRNA cassette in pGFP-V-RS (Scramble, TR30013, OriGene Technologies, Inc., Rockville, MD, USA) as control, or 4 unique 29mer shRNA constructs in retroviral GFP vector for rat PPARα shRNAs (Gene ID: 25747, TG7097836A-D, OriGene Technologies, Inc., Rockville, MD, USA). .. We obtained stably transfected clones by using antibiotic selection 0.5 μg/mL of puromycin (Sigma-Aldrich, St. Louis, MO, USA) in the culture medium for two weeks.

    Transfection:

    Article Title: S1P-S1PR1 signaling impairs CD8 + T cell metabolism and effector function in tumors
    Article Snippet: HEK 293 T (ATCC) cells were cultured in T75 tissue culture flasks (Falcon, NY, USA) under standard conditions (37 °C, 5% CO2). .. Cells were maintained in complete DMEM (Gibco, Thermo Fisher Scientific) supplemented with:10% fetal bovine serum (FBS; Gibco), 1% penicillin–streptomycin (Gibco) For lentiviral production, HEK293T cells were co-transfected with: 15 μg lentiviral expression plasmid encoding S1pr1 shRNA, Eif2ak3 shRNA, or non-targeting scrambled shRNA control (Origene, USA) 10 μg psPAX2 packaging plasmid 5 μg pMD2.G envelope plasmid Transfection was carried out using the CaCl2/HBS precipitation method. ..

    Article Title: Intellectual disability-causing mutations in KIF11 impair microtubule dynamics and dendritic arborization
    Article Snippet: .. DIV14-16 Primary hippocampal mouse neurons, plated in 35 mm Mattek No1.5 dishes were simultaneously transfected via combiMag and Lipofectamine with 0.5 μg EB3-miRFP703 (Addgene #79994) or mRuby-Synaptophysin [(pEF Synaptophysin-mRuby was a gift from Edwin Chapman (Addgene plasmid # 188980; http://n2t.net/addgene:188980 ; RRID:Addgene_188980)] or mApple-PSD95 [mApple-PSD95-N-14 was a gift from Michael Davidson (Addgene plasmid # 54941; http://n2t.net/addgene:54941 ; RRID:Addgene_54941)] and either 0.5 μg NC-GFP (Origene TR30013) or KIF11-shRNA-A (Origene TG501174) or tagged KIF11 constructs. .. After 24–48 h of transfection, EB3-miRFP703 imaging was performed at 37 °C with 5% CO 2 in TOKAI HIT STX Stage Top Incubation Chamber, using a confocal microscope (FV3000; Olympus; UPLAPO60XOHR Objective in immersion oil).

    Construct:

    Article Title: MYB/AKT3 axis is a key driver of ovarian cancer growth, aggressiveness, and chemoresistance
    Article Snippet: .. For RNA interference mediated gene silencing, four independent short hairpin RNA (shRNA) expression constructs for MYB (pGFP-V-RS-shMYB #1, #2, #3 and #4) and non-targeted scrambled control (pGFP-V-RS-NT-Scr) (Origene, Rockville, MD, USA) were used. ..

    Article Title: Intellectual disability-causing mutations in KIF11 impair microtubule dynamics and dendritic arborization
    Article Snippet: .. DIV14-16 Primary hippocampal mouse neurons, plated in 35 mm Mattek No1.5 dishes were simultaneously transfected via combiMag and Lipofectamine with 0.5 μg EB3-miRFP703 (Addgene #79994) or mRuby-Synaptophysin [(pEF Synaptophysin-mRuby was a gift from Edwin Chapman (Addgene plasmid # 188980; http://n2t.net/addgene:188980 ; RRID:Addgene_188980)] or mApple-PSD95 [mApple-PSD95-N-14 was a gift from Michael Davidson (Addgene plasmid # 54941; http://n2t.net/addgene:54941 ; RRID:Addgene_54941)] and either 0.5 μg NC-GFP (Origene TR30013) or KIF11-shRNA-A (Origene TG501174) or tagged KIF11 constructs. .. After 24–48 h of transfection, EB3-miRFP703 imaging was performed at 37 °C with 5% CO 2 in TOKAI HIT STX Stage Top Incubation Chamber, using a confocal microscope (FV3000; Olympus; UPLAPO60XOHR Objective in immersion oil).

    Article Title: Peroxisome Proliferator-Activated Receptor Family of Lipid-Activated Nuclear Receptors Alpha Silencing Promotes Oxidative Stress and Hypertrophic Phenotype in Rat Cardiac Cells
    Article Snippet: In detail, 250 ng of plasmids were transfected using Lipofectamine 2000 (Thermo Fischer Scientific, Waltham, MA, USA) following the manufacturer’s instructions. .. The following shRNA plasmids were used: 29-mer scrambled shRNA cassette in pGFP-V-RS (Scramble, TR30013, OriGene Technologies, Inc., Rockville, MD, USA) as control, or 4 unique 29mer shRNA constructs in retroviral GFP vector for rat PPARα shRNAs (Gene ID: 25747, TG7097836A-D, OriGene Technologies, Inc., Rockville, MD, USA). .. We obtained stably transfected clones by using antibiotic selection 0.5 μg/mL of puromycin (Sigma-Aldrich, St. Louis, MO, USA) in the culture medium for two weeks.

    Retroviral:

    Article Title: Peroxisome Proliferator-Activated Receptor Family of Lipid-Activated Nuclear Receptors Alpha Silencing Promotes Oxidative Stress and Hypertrophic Phenotype in Rat Cardiac Cells
    Article Snippet: In detail, 250 ng of plasmids were transfected using Lipofectamine 2000 (Thermo Fischer Scientific, Waltham, MA, USA) following the manufacturer’s instructions. .. The following shRNA plasmids were used: 29-mer scrambled shRNA cassette in pGFP-V-RS (Scramble, TR30013, OriGene Technologies, Inc., Rockville, MD, USA) as control, or 4 unique 29mer shRNA constructs in retroviral GFP vector for rat PPARα shRNAs (Gene ID: 25747, TG7097836A-D, OriGene Technologies, Inc., Rockville, MD, USA). .. We obtained stably transfected clones by using antibiotic selection 0.5 μg/mL of puromycin (Sigma-Aldrich, St. Louis, MO, USA) in the culture medium for two weeks.



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    TCF7 regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, shRNA, shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).

    Journal: International Journal of Molecular Sciences

    Article Title: Unfolding Immune Dysregulation in COPD: Identification of a Three-Gene Signature and Functional Validation of TCF7 in Human Lung Tissue and T Lymphocytes

    doi: 10.3390/ijms27104231

    Figure Lengend Snippet: TCF7 regulates pro−caspase−8 expression in T lymphocytes and is significantly reduced in COPD. ( A ) Immunofluorescence co−staining of control human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Scale bar is 50 μm. ( B ) Immunofluorescence co−staining of COPD human lung tissue displaying separate channels for DAPI (blue), caspase−8 (green), TCF7 (red), and the merged image. Note the marked reduction in both TCF7 and caspase−8 signals compared to the control. Scale bar is 50 μm. ( C ) Representative Western blot images of TCF7 (50 kDa), pro−caspase−8 (55 kDa), and internal control β−tubulin (55 kDa) in wild type (WT) and TCF7 knockout (KO) Jurkat T cells. ( D ) Quantitative densitometric analysis of TCF7 protein levels comparing WT and KO groups. ( E ) Quantitative densitometric analysis of pro−caspase−8 protein levels comparing WT and KO groups. ( F ) Representative Western blot images of TCF7 and β−tubulin in primary T lymphocytes isolated from the peripheral blood of healthy donors (Control) and patients with COPD (Model). ( G ) Quantitative densitometric analysis of TCF7 protein levels in human primary T lymphocytes. ( H ) Representative Western blot images of TCF7 and β−tubulin protein levels in Jurkat T cells across four experimental conditions including Control, shRNA, shRNA plus TCF7 Rescue construct, and shRNA plus Empty Vector. ( I ) Quantitative densitometric analysis of TCF7 protein levels across the four experimental rescue groups. ( J ) Representative Western blot images of pro−caspase−8 and β−tubulin protein levels across the same four experimental conditions in Jurkat T cells. ( K ) Quantitative densitometric analysis of pro−caspase−8 protein levels across the four experimental rescue groups. Data in the bar charts are presented as mean ± SD ( n = 4 for primary human cells, n = 3 for cell line experiments). Statistical significance was assessed using Student’s t test with Welch’s correction where appropriate (* p < 0.05, *** p < 0.001, ns indicates not significant).

    Article Snippet: Short hairpin RNA targeting human TCF7 (shRNA) and a scramble control shRNA were purchased from OriGene with Cat.No.TR30004.

    Techniques: Expressing, Immunofluorescence, Staining, Control, Western Blot, Knock-Out, Isolation, shRNA, Construct, Plasmid Preparation