Solvent:Article Title: p53 reveals principles of chromatin remodeling and enhancer activation.
Article Snippet: .. Cells were treated with Dimethylsulfoxid (DMSO) solvent control (0.15%; Carl Roth, Karlsruhe, Germany) or Nutlin-3a (10 μM; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. Transfection, RNA extraction, and reverse transcription semi-quantitative real-time PCR Cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (#4390844 and #s607 from Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific) and Opti-MEM (Thermo Fisher Scientific) following the manufacturer’s protocol. .. Total cellular RNA was extracted using the innuPREP RNA Mini Kit (IST Innuscreen, Berlin, Germany) following the manufacturer’s protocol.
Article Title: Gene regulation by convergent promoters.
Article Snippet: .. Cells were treated with DMSO solvent control (0.15%; Carl Roth) or Nutlin-3a (10 μM; Sigma Aldrich) for 24 h. For knockdown experiments, cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (ThermoFisher Scientific) using RNAiMAX (ThermoFisher Scientific) and Opti-MEM (ThermoFisher Scientific) following the manufacturerʼ protocol. .. The following silencer select siRNAs (ThermoFisher Scientific) were used: siControl (cat. no. 4390844), FAS (cat. no. s1508), ACTA2 (cat. no. s945), da_PTP4A1 (forward CCACGUUUCUCAUAAUUAAtt; reverse UUAAUUAUGAGAAACGUGGtt).
Control:Article Title: p53 reveals principles of chromatin remodeling and enhancer activation.
Article Snippet: .. Cells were treated with Dimethylsulfoxid (DMSO) solvent control (0.15%; Carl Roth, Karlsruhe, Germany) or Nutlin-3a (10 μM; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. Transfection, RNA extraction, and reverse transcription semi-quantitative real-time PCR Cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (#4390844 and #s607 from Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific) and Opti-MEM (Thermo Fisher Scientific) following the manufacturer’s protocol. .. Total cellular RNA was extracted using the innuPREP RNA Mini Kit (IST Innuscreen, Berlin, Germany) following the manufacturer’s protocol.
Article Title: Gene regulation by convergent promoters.
Article Snippet: .. Cells were treated with DMSO solvent control (0.15%; Carl Roth) or Nutlin-3a (10 μM; Sigma Aldrich) for 24 h. For knockdown experiments, cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (ThermoFisher Scientific) using RNAiMAX (ThermoFisher Scientific) and Opti-MEM (ThermoFisher Scientific) following the manufacturerʼ protocol. .. The following silencer select siRNAs (ThermoFisher Scientific) were used: siControl (cat. no. 4390844), FAS (cat. no. s1508), ACTA2 (cat. no. s945), da_PTP4A1 (forward CCACGUUUCUCAUAAUUAAtt; reverse UUAAUUAUGAGAAACGUGGtt).
Transfection:Article Title: p53 reveals principles of chromatin remodeling and enhancer activation.
Article Snippet: .. Cells were treated with Dimethylsulfoxid (DMSO) solvent control (0.15%; Carl Roth, Karlsruhe, Germany) or Nutlin-3a (10 μM; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. Transfection, RNA extraction, and reverse transcription semi-quantitative real-time PCR Cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (#4390844 and #s607 from Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific) and Opti-MEM (Thermo Fisher Scientific) following the manufacturer’s protocol. .. Total cellular RNA was extracted using the innuPREP RNA Mini Kit (IST Innuscreen, Berlin, Germany) following the manufacturer’s protocol.
Article Title: Gene regulation by convergent promoters.
Article Snippet: .. Cells were treated with DMSO solvent control (0.15%; Carl Roth) or Nutlin-3a (10 μM; Sigma Aldrich) for 24 h. For knockdown experiments, cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (ThermoFisher Scientific) using RNAiMAX (ThermoFisher Scientific) and Opti-MEM (ThermoFisher Scientific) following the manufacturerʼ protocol. .. The following silencer select siRNAs (ThermoFisher Scientific) were used: siControl (cat. no. 4390844), FAS (cat. no. s1508), ACTA2 (cat. no. s945), da_PTP4A1 (forward CCACGUUUCUCAUAAUUAAtt; reverse UUAAUUAUGAGAAACGUGGtt).
RNA Extraction:Article Title: p53 reveals principles of chromatin remodeling and enhancer activation.
Article Snippet: .. Cells were treated with Dimethylsulfoxid (DMSO) solvent control (0.15%; Carl Roth, Karlsruhe, Germany) or Nutlin-3a (10 μM; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. Transfection, RNA extraction, and reverse transcription semi-quantitative real-time PCR Cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (#4390844 and #s607 from Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific) and Opti-MEM (Thermo Fisher Scientific) following the manufacturer’s protocol. .. Total cellular RNA was extracted using the innuPREP RNA Mini Kit (IST Innuscreen, Berlin, Germany) following the manufacturer’s protocol.
Reverse Transcription:Article Title: p53 reveals principles of chromatin remodeling and enhancer activation.
Article Snippet: .. Cells were treated with Dimethylsulfoxid (DMSO) solvent control (0.15%; Carl Roth, Karlsruhe, Germany) or Nutlin-3a (10 μM; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. Transfection, RNA extraction, and reverse transcription semi-quantitative real-time PCR Cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (#4390844 and #s607 from Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific) and Opti-MEM (Thermo Fisher Scientific) following the manufacturer’s protocol. .. Total cellular RNA was extracted using the innuPREP RNA Mini Kit (IST Innuscreen, Berlin, Germany) following the manufacturer’s protocol.
Real-time Polymerase Chain Reaction:Article Title: p53 reveals principles of chromatin remodeling and enhancer activation.
Article Snippet: .. Cells were treated with Dimethylsulfoxid (DMSO) solvent control (0.15%; Carl Roth, Karlsruhe, Germany) or Nutlin-3a (10 μM; MedChemExpress, Monmouth Junction, NJ, USA) for 24 h. Transfection, RNA extraction, and reverse transcription semi-quantitative real-time PCR Cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (#4390844 and #s607 from Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific) and Opti-MEM (Thermo Fisher Scientific) following the manufacturer’s protocol. .. Total cellular RNA was extracted using the innuPREP RNA Mini Kit (IST Innuscreen, Berlin, Germany) following the manufacturer’s protocol.
Knockdown:Article Title: Gene regulation by convergent promoters.
Article Snippet: .. Cells were treated with DMSO solvent control (0.15%; Carl Roth) or Nutlin-3a (10 μM; Sigma Aldrich) for 24 h. For knockdown experiments, cells were seeded in six-well plates and reverse transfected with 10 nM Silencer Select siRNAs (ThermoFisher Scientific) using RNAiMAX (ThermoFisher Scientific) and Opti-MEM (ThermoFisher Scientific) following the manufacturerʼ protocol. .. The following silencer select siRNAs (ThermoFisher Scientific) were used: siControl (cat. no. 4390844), FAS (cat. no. s1508), ACTA2 (cat. no. s945), da_PTP4A1 (forward CCACGUUUCUCAUAAUUAAtt; reverse UUAAUUAUGAGAAACGUGGtt).
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