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control shrna lentiviral particles a  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology control shrna lentiviral particles a
    Control Shrna Lentiviral Particles A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+shrna/Control+shRNA+Lentiviral+Particles-A/pm41896541-77-0-8
    Average 96 stars, based on 1301 article reviews
    control shrna lentiviral particles a - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    shRNA:

    Article Title: METTL16 supports arginine biosynthesis and suppresses IL-1β production to attenuate allergic asthma
    Article Snippet: Upon reaching 50% confluency, the cells were incubated with complete growth medium containing 5 μg/mL polybrene (Yeasen, 40804ES76, Shanghai, China). .. The cells were then transducedwith either METTL16-targeting shRNA lentiviruses (Santa Cruz Biotechnology, sc-149379-V, CA, USA) or nontargeting control shRNA lentiviruses (Santa Cruz Biotechnology, sc-108080, CA, USA). ..

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).

    Article Title: CXCR4, CXCR7 and PBRM1 are responsible for everolimus and cabozantinib resistance in human renal cancer cells.
    Article Snippet: .. Control shRNA Lentiviral Particles-A (2.5x104 IFU; shControl, sc-108080, Santa Cruz Biotechnology, Dallas, TX, USA) were used. ..

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle
    Article Snippet: GsMTx4 (Pz inhibitor; HY-P1410) was purchased from MedChemExpress (NJ, USA). .. Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA). ..

    Article Title: Adrenomedullin 2 drives intratumoral cancer cell invasion, intravasation and metastasis.
    Article Snippet: .. Scramble non targeting shRNA (sc-108080), ADM2 (sc-72452-V) and ATF2 (sc-29205-V) were purchased from Santa Cruz Biotechnologies. .. Lectin-649 (VECTDL1048) was from Vector Laboratories.

    Article Title: A natural product-derived degrader (COT-01): cortisone-based CRBN recruitment for cyclin D2 degradation in multiple myeloma.
    Article Snippet: .. MM.1S cells were transduced with lentiviral vectors encoding either a non-targeting control shRNA or an shRNA targeting human CRBN (sc108080, sc-78528-V, Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Control:

    Article Title: METTL16 supports arginine biosynthesis and suppresses IL-1β production to attenuate allergic asthma
    Article Snippet: Upon reaching 50% confluency, the cells were incubated with complete growth medium containing 5 μg/mL polybrene (Yeasen, 40804ES76, Shanghai, China). .. The cells were then transducedwith either METTL16-targeting shRNA lentiviruses (Santa Cruz Biotechnology, sc-149379-V, CA, USA) or nontargeting control shRNA lentiviruses (Santa Cruz Biotechnology, sc-108080, CA, USA). ..

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).

    Article Title: CXCR4, CXCR7 and PBRM1 are responsible for everolimus and cabozantinib resistance in human renal cancer cells.
    Article Snippet: .. Control shRNA Lentiviral Particles-A (2.5x104 IFU; shControl, sc-108080, Santa Cruz Biotechnology, Dallas, TX, USA) were used. ..

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle
    Article Snippet: GsMTx4 (Pz inhibitor; HY-P1410) was purchased from MedChemExpress (NJ, USA). .. Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA). ..

    Article Title: A natural product-derived degrader (COT-01): cortisone-based CRBN recruitment for cyclin D2 degradation in multiple myeloma.
    Article Snippet: .. MM.1S cells were transduced with lentiviral vectors encoding either a non-targeting control shRNA or an shRNA targeting human CRBN (sc108080, sc-78528-V, Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Transduction:

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).

    Article Title: A natural product-derived degrader (COT-01): cortisone-based CRBN recruitment for cyclin D2 degradation in multiple myeloma.
    Article Snippet: .. MM.1S cells were transduced with lentiviral vectors encoding either a non-targeting control shRNA or an shRNA targeting human CRBN (sc108080, sc-78528-V, Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Sequencing:

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Knockdown:

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).



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    Santa Cruz Biotechnology control shrna lentiviral particles a
    Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
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    Image Search Results


    Relationship between MIF and TMED10. (A) Secretion of MIF was measured in HEK293T cells either untreated or treated with brefeldin A. (B) Secretion of MIF was measured in HEK293T cells untreated or treated with the indicated doses of probenecid. (C) GST or the indicated GST-TMED10 domains coupled to glutathione sepharose beads were incubated with whole cell lysates from HEK293T cells expressing Flag-MIF. Elutes were subjected to SDS-PAGE and immunoblotted with an anti-Flag antibody. (D) GST-MIF coupled to glutathione sepharose beads was incubated with whole cell lysate from HEK293T cells expressing GFP-TMED10 or GFP-TMED10(ΔCT). Elutes were subjected to SDS-PAGE and immunoblotted with anti-GFP antibody. (E) Secretion of MIF was measured in HEK293T cells expressing mock or GFP-TMED10. (F) Secretion of MIF was measured in HEK293T cells treated with control siRNA or siRNA targeting TMED10 for 72 h. (G) A crosslink assay was performed in HEK293T cells expressing GFP-TMED10 with mock or Flag-MIF to examine the oligomeric form of TMED10. (H) Secretion of Flag-MIF was measured in HEK293T cells expressing mock, RAB1A, 1B, and 2A.

    Journal: Autophagy Reports

    Article Title: TRPML3 and TRPML1/3 differentially regulate unconventional MIF secretion through TMED10 and secretory autophagy

    doi: 10.1080/27694127.2026.2740425

    Figure Lengend Snippet: Relationship between MIF and TMED10. (A) Secretion of MIF was measured in HEK293T cells either untreated or treated with brefeldin A. (B) Secretion of MIF was measured in HEK293T cells untreated or treated with the indicated doses of probenecid. (C) GST or the indicated GST-TMED10 domains coupled to glutathione sepharose beads were incubated with whole cell lysates from HEK293T cells expressing Flag-MIF. Elutes were subjected to SDS-PAGE and immunoblotted with an anti-Flag antibody. (D) GST-MIF coupled to glutathione sepharose beads was incubated with whole cell lysate from HEK293T cells expressing GFP-TMED10 or GFP-TMED10(ΔCT). Elutes were subjected to SDS-PAGE and immunoblotted with anti-GFP antibody. (E) Secretion of MIF was measured in HEK293T cells expressing mock or GFP-TMED10. (F) Secretion of MIF was measured in HEK293T cells treated with control siRNA or siRNA targeting TMED10 for 72 h. (G) A crosslink assay was performed in HEK293T cells expressing GFP-TMED10 with mock or Flag-MIF to examine the oligomeric form of TMED10. (H) Secretion of Flag-MIF was measured in HEK293T cells expressing mock, RAB1A, 1B, and 2A.

    Article Snippet: For knockdown experiments, siRNA targeting human TMED10 (BIONEER, 10,972–1), human ATG5 (BIONEER, 9474–1), human SYT5 (BIONEER, 6861–1), and a negative control scrambled siRNA (Santa Cruz Biotechnology, sc-37007) were used.

    Techniques: Incubation, Expressing, SDS Page, Control

    Effect of autophagy on MIF secretion. (A) HeLa cells expressing GFP-LC3 and mCherry-MIF were treated with cold digitonin (50 µg/ml) and imaged by fluorescence microscopy. (B) Secretion of MIF was measured in HEK293T cells either untreated or treated with 10 μM SAR405. (C) Secretion of MIF was measured in HEK293T cells treated with control siRNA or siRNA targeting ATG5 for 72 h. (D) Secretion of MIF was measured in HEK293T cells either untreated or treated with 100 nM bafilomycin A1. (E) Secretion of MIF was measured in HEK293T cells treated with control siRNA or siRNA targeting SYT5 for 48 h. Knockdown efficiency was confirmed by immunoblotting for SYT5. (F) Secretion of Flag-MIF was measured in HEK293T cells expressing mock or GFP-SEC22B. (G) HeLa cells expressing GFP-SEC22B and mCherry-MIF were treated with cold digitonin (50 µg/ml) and imaged by fluorescence microscopy. (H, upper) HeLa cells co-expressing GFP-MIF and Flag-VAMP4 were treated with cold digitonin (50 µg/ml), immunostained with an anti-Flag antibody, and imaged by fluorescence microscopy. (H, lower) HeLa cells expressing GFP-VAMP8 and mCherry-MIF were treated with cold digitonin (50 µg/ml) and imaged by fluorescence microscopy.

    Journal: Autophagy Reports

    Article Title: TRPML3 and TRPML1/3 differentially regulate unconventional MIF secretion through TMED10 and secretory autophagy

    doi: 10.1080/27694127.2026.2740425

    Figure Lengend Snippet: Effect of autophagy on MIF secretion. (A) HeLa cells expressing GFP-LC3 and mCherry-MIF were treated with cold digitonin (50 µg/ml) and imaged by fluorescence microscopy. (B) Secretion of MIF was measured in HEK293T cells either untreated or treated with 10 μM SAR405. (C) Secretion of MIF was measured in HEK293T cells treated with control siRNA or siRNA targeting ATG5 for 72 h. (D) Secretion of MIF was measured in HEK293T cells either untreated or treated with 100 nM bafilomycin A1. (E) Secretion of MIF was measured in HEK293T cells treated with control siRNA or siRNA targeting SYT5 for 48 h. Knockdown efficiency was confirmed by immunoblotting for SYT5. (F) Secretion of Flag-MIF was measured in HEK293T cells expressing mock or GFP-SEC22B. (G) HeLa cells expressing GFP-SEC22B and mCherry-MIF were treated with cold digitonin (50 µg/ml) and imaged by fluorescence microscopy. (H, upper) HeLa cells co-expressing GFP-MIF and Flag-VAMP4 were treated with cold digitonin (50 µg/ml), immunostained with an anti-Flag antibody, and imaged by fluorescence microscopy. (H, lower) HeLa cells expressing GFP-VAMP8 and mCherry-MIF were treated with cold digitonin (50 µg/ml) and imaged by fluorescence microscopy.

    Article Snippet: For knockdown experiments, siRNA targeting human TMED10 (BIONEER, 10,972–1), human ATG5 (BIONEER, 9474–1), human SYT5 (BIONEER, 6861–1), and a negative control scrambled siRNA (Santa Cruz Biotechnology, sc-37007) were used.

    Techniques: Expressing, Fluorescence, Microscopy, Control, Knockdown, Western Blot

    Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle

    doi: 10.1093/ajrcmb/aanag058

    Figure Lengend Snippet: Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

    Article Snippet: Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA).

    Techniques: CyQUANT Assay, Comparison, Expressing, shRNA, Knockdown, Western Blot, Analysis