sequence (Santa Cruz Biotechnology)
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Sequence, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 859 article reviews
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shRNA:Article Title: Deciphering the role of SIRT6 in suppressing the AMPK-mTOR-TFEB axis: regulation of autophagy activation in HCC. Article Snippet: Sirtuin 6 (SIRT6), belong to the NAD-dependent class III protein deacetylase family, is implicated in cancer development through a multifaceted role.. While it has been identified with both tumor-suppressive and tumor-promoting roles in Hepatocellular carcinoma (HCC), there remains considerable debate regarding its exact function.. The specific molecular mechanisms driving its tumor-suppressive effects in HCC remains poorly understood. Article Title: HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors Article Snippet: .. U87MG cells were transfected with either TRPML1 shRNA plasmid (Santa Cruz, Sc-44519) or Article Title: TREM1-NLRP3-driven pyroptosis in sepsis-associated acute kidney injury (AKI) with parallel autophagy changes. Article Snippet: .. For the depletion of TREM-1, TREM-1 siRNA (Cat# sc-42999, Santa Cruz Biotechnology, USA), Article Title: FTO-Mediated m6A Demethylation of MZF1 Regulates DECR1 to Promote Fatty Acid Oxidation and Exacerbate Myocardial Ischemia/Reperfusion Injury : FTO-Mediated m6A Demethylation of MZF1 Enhances Fatty Acid Oxidation and Aggravates Myocardial I/R Injury. Article Snippet: Myocardial ischemia-reperfusion injury (MIRI) is a major clinical challenge, marked by metabolic disruptions and cellular damage following the restoration of blood flow after ischemia.. During ischemia, the heart shifts from fatty acid metabolism to glucose metabolism, leading to metabolic abnormalities, including reduced intracellular pH, ion disturbances, cell swelling, and apoptosis.. Upon reperfusion, fatty acid β-oxidation resumes, becoming the dominant energy source, which induces excessive oxidative stress and aggravates myocardial injury. Article Title: Dysregulation of a novel autophagosome-mitochondria contact contributes to autophagy dysfunction and neurodegeneration in tauopathy Article Snippet: .. Sources of antibodies or reagents are as follows: polyclonal antibodies against LC3 (Cell Signaling Technology, Cat# 2775), AMPKα (Cell Signaling Technology, Cat# 2532), p62/SQSTM1 (Abnova, Cat# H00008878-M01), NeuN (Millipore/Sigma, Cat# ABN78), TBC1D15 (Abcam, Cat# ab121396), Tau5 (DAKO, Cat# A0024), and synaptophysin/SYP (Abcam, Cat# ab32127); monoclonal antibodies against phospho-AMPKα (Cell Signaling Technology, Cat# 2535), AT8 (ThermoFisher Scientific, Cat# MN1020), syntaxin 1/STX1 (Santa Cruz Biotechnology, Cat# sc-12736), Rab7 (Sigma, Cat# R8779), GAPDH (Sigma, Cat# CB1001), TOM20 (Abcam, Cat# ab186734), mCherry (Takara, Cat# 632543), and Alexa fluor 488- (Cat# A-11017; Cat# A-11070), 546- (Cat# A-11018; Cat# A-11071), and 647- (Cat# A-21237; Cat# A-21246) conjugated secondary antibodies (Invitrogen); 10-NCP (VWR, Cat# 80017-188), trehalose (Cat# T0167), cycloheximide (Cat# 01810), rotenone (Cat# 557368), antimycin A (Cat# 1397-94-0), AICAR (Cat# A9978), CC (Cat# 171264), epoxomicin (Cat# 324800), CID 1067700 (Cat# SML0545), DMSO (Cat# D2650), and Sarkosyl (Cat# L9150) (Sigma); TBC1D15 shRNA (Cat# sc-154093-SH), Fis1 shRNA (Cat# sc-60644-SH), and Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors. Article Snippet: .. Transfection of small interfering RNA (siRNA) or short hairpin RNA (shRNA) Transfection of an siRNA targeting either PD-L1 (ON-TARGETplus siRNA for CD274) (L-015836-01-0010, Horizon Discovery, USA) or importin-α3 (ON-TARGETplus siRNA for KPNA4) (L-017477-00-0010, Horizon Discovery, USA) or importin-β1 (ON-TARGETplus siRNA for KPNB1) (L-017523-00-0010, Horizon Discovery, USA) or TAZ (ON-TARGETplus siRNA for WWTR1) (L-016083-00-0010, Horizon Discovery, USA) was performed as described previously.37 The shRNA targeting YAP (sc-38637-SH, Santa Cruz Biotechnology, USA) or the Article Title: Obestatin Treatment Counteracts Muscle Wasting by Reactivation of Autophagy in Duchenne Muscular Dystrophy Article Snippet: To knockdown NEDD4, AMPKα or USP10 expression, siRNA specifically targeting human NEDD4‐L (sc‐75894; Santa Cruz Biotechnology, CA, USA), AMPKα (sc‐45312; Santa Cruz Biotechnology), or USP10 (sc‐76811; Santa Cruz Biotechnology) was used. siRNA duplexes targeting preproghrelin or c‐Src were selected from ON‐TARGET plus SMARTpool siRNA from Thermo Fisher Scientific (Dharmacon, CO, USA). shRNA‐mediated gene silencing was used for ULK1 knockdown (sc‐44182‐SH; Santa Cruz Biotechnology) in DMD cells. .. An ON‐TARGETplus nontargeting siRNA (Dharmacon) or Article Title: Involvement of SWAP-70 in proteolipid protein-induced experimental autoimmune encephalomyelitis. Article Snippet: To silence the SWAP-70 gene, a lentiviral particle carrying shRNA (sc-153963-V, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased. .. A Plasmid Preparation:Article Title: Deciphering the role of SIRT6 in suppressing the AMPK-mTOR-TFEB axis: regulation of autophagy activation in HCC. Article Snippet: Sirtuin 6 (SIRT6), belong to the NAD-dependent class III protein deacetylase family, is implicated in cancer development through a multifaceted role.. While it has been identified with both tumor-suppressive and tumor-promoting roles in Hepatocellular carcinoma (HCC), there remains considerable debate regarding its exact function.. The specific molecular mechanisms driving its tumor-suppressive effects in HCC remains poorly understood. Article Title: HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors Article Snippet: .. U87MG cells were transfected with either TRPML1 shRNA plasmid (Santa Cruz, Sc-44519) or Article Title: TREM1-NLRP3-driven pyroptosis in sepsis-associated acute kidney injury (AKI) with parallel autophagy changes. Article Snippet: .. For the depletion of TREM-1, TREM-1 siRNA (Cat# sc-42999, Santa Cruz Biotechnology, USA), Article Title: Obestatin Treatment Counteracts Muscle Wasting by Reactivation of Autophagy in Duchenne Muscular Dystrophy Article Snippet: To knockdown NEDD4, AMPKα or USP10 expression, siRNA specifically targeting human NEDD4‐L (sc‐75894; Santa Cruz Biotechnology, CA, USA), AMPKα (sc‐45312; Santa Cruz Biotechnology), or USP10 (sc‐76811; Santa Cruz Biotechnology) was used. siRNA duplexes targeting preproghrelin or c‐Src were selected from ON‐TARGET plus SMARTpool siRNA from Thermo Fisher Scientific (Dharmacon, CO, USA). shRNA‐mediated gene silencing was used for ULK1 knockdown (sc‐44182‐SH; Santa Cruz Biotechnology) in DMD cells. .. An ON‐TARGETplus nontargeting siRNA (Dharmacon) or Article Title: Involvement of SWAP-70 in proteolipid protein-induced experimental autoimmune encephalomyelitis. Article Snippet: To silence the SWAP-70 gene, a lentiviral particle carrying shRNA (sc-153963-V, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased. .. A Transfection:Article Title: Deciphering the role of SIRT6 in suppressing the AMPK-mTOR-TFEB axis: regulation of autophagy activation in HCC. Article Snippet: Sirtuin 6 (SIRT6), belong to the NAD-dependent class III protein deacetylase family, is implicated in cancer development through a multifaceted role.. While it has been identified with both tumor-suppressive and tumor-promoting roles in Hepatocellular carcinoma (HCC), there remains considerable debate regarding its exact function.. The specific molecular mechanisms driving its tumor-suppressive effects in HCC remains poorly understood. Article Title: HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors Article Snippet: .. U87MG cells were transfected with either TRPML1 shRNA plasmid (Santa Cruz, Sc-44519) or Article Title: TREM1-NLRP3-driven pyroptosis in sepsis-associated acute kidney injury (AKI) with parallel autophagy changes. Article Snippet: .. For the depletion of TREM-1, TREM-1 siRNA (Cat# sc-42999, Santa Cruz Biotechnology, USA), Article Title: FTO-Mediated m6A Demethylation of MZF1 Regulates DECR1 to Promote Fatty Acid Oxidation and Exacerbate Myocardial Ischemia/Reperfusion Injury : FTO-Mediated m6A Demethylation of MZF1 Enhances Fatty Acid Oxidation and Aggravates Myocardial I/R Injury. Article Snippet: Myocardial ischemia-reperfusion injury (MIRI) is a major clinical challenge, marked by metabolic disruptions and cellular damage following the restoration of blood flow after ischemia.. During ischemia, the heart shifts from fatty acid metabolism to glucose metabolism, leading to metabolic abnormalities, including reduced intracellular pH, ion disturbances, cell swelling, and apoptosis.. Upon reperfusion, fatty acid β-oxidation resumes, becoming the dominant energy source, which induces excessive oxidative stress and aggravates myocardial injury. Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors. Article Snippet: .. Transfection of small interfering RNA (siRNA) or short hairpin RNA (shRNA) Transfection of an siRNA targeting either PD-L1 (ON-TARGETplus siRNA for CD274) (L-015836-01-0010, Horizon Discovery, USA) or importin-α3 (ON-TARGETplus siRNA for KPNA4) (L-017477-00-0010, Horizon Discovery, USA) or importin-β1 (ON-TARGETplus siRNA for KPNB1) (L-017523-00-0010, Horizon Discovery, USA) or TAZ (ON-TARGETplus siRNA for WWTR1) (L-016083-00-0010, Horizon Discovery, USA) was performed as described previously.37 The shRNA targeting YAP (sc-38637-SH, Santa Cruz Biotechnology, USA) or the Control:Article Title: Deciphering the role of SIRT6 in suppressing the AMPK-mTOR-TFEB axis: regulation of autophagy activation in HCC. Article Snippet: Sirtuin 6 (SIRT6), belong to the NAD-dependent class III protein deacetylase family, is implicated in cancer development through a multifaceted role.. While it has been identified with both tumor-suppressive and tumor-promoting roles in Hepatocellular carcinoma (HCC), there remains considerable debate regarding its exact function.. The specific molecular mechanisms driving its tumor-suppressive effects in HCC remains poorly understood. Article Title: HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors Article Snippet: .. U87MG cells were transfected with either TRPML1 shRNA plasmid (Santa Cruz, Sc-44519) or Article Title: TREM1-NLRP3-driven pyroptosis in sepsis-associated acute kidney injury (AKI) with parallel autophagy changes. Article Snippet: .. For the depletion of TREM-1, TREM-1 siRNA (Cat# sc-42999, Santa Cruz Biotechnology, USA), Article Title: FTO-Mediated m6A Demethylation of MZF1 Regulates DECR1 to Promote Fatty Acid Oxidation and Exacerbate Myocardial Ischemia/Reperfusion Injury : FTO-Mediated m6A Demethylation of MZF1 Enhances Fatty Acid Oxidation and Aggravates Myocardial I/R Injury. Article Snippet: Myocardial ischemia-reperfusion injury (MIRI) is a major clinical challenge, marked by metabolic disruptions and cellular damage following the restoration of blood flow after ischemia.. During ischemia, the heart shifts from fatty acid metabolism to glucose metabolism, leading to metabolic abnormalities, including reduced intracellular pH, ion disturbances, cell swelling, and apoptosis.. Upon reperfusion, fatty acid β-oxidation resumes, becoming the dominant energy source, which induces excessive oxidative stress and aggravates myocardial injury. Article Title: Dysregulation of a novel autophagosome-mitochondria contact contributes to autophagy dysfunction and neurodegeneration in tauopathy Article Snippet: .. Sources of antibodies or reagents are as follows: polyclonal antibodies against LC3 (Cell Signaling Technology, Cat# 2775), AMPKα (Cell Signaling Technology, Cat# 2532), p62/SQSTM1 (Abnova, Cat# H00008878-M01), NeuN (Millipore/Sigma, Cat# ABN78), TBC1D15 (Abcam, Cat# ab121396), Tau5 (DAKO, Cat# A0024), and synaptophysin/SYP (Abcam, Cat# ab32127); monoclonal antibodies against phospho-AMPKα (Cell Signaling Technology, Cat# 2535), AT8 (ThermoFisher Scientific, Cat# MN1020), syntaxin 1/STX1 (Santa Cruz Biotechnology, Cat# sc-12736), Rab7 (Sigma, Cat# R8779), GAPDH (Sigma, Cat# CB1001), TOM20 (Abcam, Cat# ab186734), mCherry (Takara, Cat# 632543), and Alexa fluor 488- (Cat# A-11017; Cat# A-11070), 546- (Cat# A-11018; Cat# A-11071), and 647- (Cat# A-21237; Cat# A-21246) conjugated secondary antibodies (Invitrogen); 10-NCP (VWR, Cat# 80017-188), trehalose (Cat# T0167), cycloheximide (Cat# 01810), rotenone (Cat# 557368), antimycin A (Cat# 1397-94-0), AICAR (Cat# A9978), CC (Cat# 171264), epoxomicin (Cat# 324800), CID 1067700 (Cat# SML0545), DMSO (Cat# D2650), and Sarkosyl (Cat# L9150) (Sigma); TBC1D15 shRNA (Cat# sc-154093-SH), Fis1 shRNA (Cat# sc-60644-SH), and Article Title: Obestatin Treatment Counteracts Muscle Wasting by Reactivation of Autophagy in Duchenne Muscular Dystrophy Article Snippet: To knockdown NEDD4, AMPKα or USP10 expression, siRNA specifically targeting human NEDD4‐L (sc‐75894; Santa Cruz Biotechnology, CA, USA), AMPKα (sc‐45312; Santa Cruz Biotechnology), or USP10 (sc‐76811; Santa Cruz Biotechnology) was used. siRNA duplexes targeting preproghrelin or c‐Src were selected from ON‐TARGET plus SMARTpool siRNA from Thermo Fisher Scientific (Dharmacon, CO, USA). shRNA‐mediated gene silencing was used for ULK1 knockdown (sc‐44182‐SH; Santa Cruz Biotechnology) in DMD cells. .. An ON‐TARGETplus nontargeting siRNA (Dharmacon) or Article Title: Involvement of SWAP-70 in proteolipid protein-induced experimental autoimmune encephalomyelitis. Article Snippet: To silence the SWAP-70 gene, a lentiviral particle carrying shRNA (sc-153963-V, Santa Cruz Biotechnology, Dallas, TX, USA) was purchased. .. A Negative Control:Article Title: TREM1-NLRP3-driven pyroptosis in sepsis-associated acute kidney injury (AKI) with parallel autophagy changes. Article Snippet: .. For the depletion of TREM-1, TREM-1 siRNA (Cat# sc-42999, Santa Cruz Biotechnology, USA), Bioprocessing:Article Title: Dysregulation of a novel autophagosome-mitochondria contact contributes to autophagy dysfunction and neurodegeneration in tauopathy Article Snippet: .. Sources of antibodies or reagents are as follows: polyclonal antibodies against LC3 (Cell Signaling Technology, Cat# 2775), AMPKα (Cell Signaling Technology, Cat# 2532), p62/SQSTM1 (Abnova, Cat# H00008878-M01), NeuN (Millipore/Sigma, Cat# ABN78), TBC1D15 (Abcam, Cat# ab121396), Tau5 (DAKO, Cat# A0024), and synaptophysin/SYP (Abcam, Cat# ab32127); monoclonal antibodies against phospho-AMPKα (Cell Signaling Technology, Cat# 2535), AT8 (ThermoFisher Scientific, Cat# MN1020), syntaxin 1/STX1 (Santa Cruz Biotechnology, Cat# sc-12736), Rab7 (Sigma, Cat# R8779), GAPDH (Sigma, Cat# CB1001), TOM20 (Abcam, Cat# ab186734), mCherry (Takara, Cat# 632543), and Alexa fluor 488- (Cat# A-11017; Cat# A-11070), 546- (Cat# A-11018; Cat# A-11071), and 647- (Cat# A-21237; Cat# A-21246) conjugated secondary antibodies (Invitrogen); 10-NCP (VWR, Cat# 80017-188), trehalose (Cat# T0167), cycloheximide (Cat# 01810), rotenone (Cat# 557368), antimycin A (Cat# 1397-94-0), AICAR (Cat# A9978), CC (Cat# 171264), epoxomicin (Cat# 324800), CID 1067700 (Cat# SML0545), DMSO (Cat# D2650), and Sarkosyl (Cat# L9150) (Sigma); TBC1D15 shRNA (Cat# sc-154093-SH), Fis1 shRNA (Cat# sc-60644-SH), and Small Interfering RNA:Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors. Article Snippet: .. Transfection of small interfering RNA (siRNA) or short hairpin RNA (shRNA) Transfection of an siRNA targeting either PD-L1 (ON-TARGETplus siRNA for CD274) (L-015836-01-0010, Horizon Discovery, USA) or importin-α3 (ON-TARGETplus siRNA for KPNA4) (L-017477-00-0010, Horizon Discovery, USA) or importin-β1 (ON-TARGETplus siRNA for KPNB1) (L-017523-00-0010, Horizon Discovery, USA) or TAZ (ON-TARGETplus siRNA for WWTR1) (L-016083-00-0010, Horizon Discovery, USA) was performed as described previously.37 The shRNA targeting YAP (sc-38637-SH, Santa Cruz Biotechnology, USA) or the Multiple Displacement Amplification:Article Title: Nuclear PD-L1 regulates YAP-driven transcription via the PGE 2 -EP4-YAP-importin α3 axis in solid tumors. Article Snippet: .. Transfection of small interfering RNA (siRNA) or short hairpin RNA (shRNA) Transfection of an siRNA targeting either PD-L1 (ON-TARGETplus siRNA for CD274) (L-015836-01-0010, Horizon Discovery, USA) or importin-α3 (ON-TARGETplus siRNA for KPNA4) (L-017477-00-0010, Horizon Discovery, USA) or importin-β1 (ON-TARGETplus siRNA for KPNB1) (L-017523-00-0010, Horizon Discovery, USA) or TAZ (ON-TARGETplus siRNA for WWTR1) (L-016083-00-0010, Horizon Discovery, USA) was performed as described previously.37 The shRNA targeting YAP (sc-38637-SH, Santa Cruz Biotechnology, USA) or the |
