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control lentiviral particles shctrl  (Genechem)

 
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    Structured Review

    Genechem control lentiviral particles shctrl
    Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in <t>T24-shCtrl</t> and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance
    Control Lentiviral Particles Shctrl, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+shctrl/lentiviral+particles/pmc12462137-114-6-13
    Average 86 stars, based on 1 article reviews
    control lentiviral particles shctrl - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "CCDC137 knockdown suppresses bladder cancer progression by downregulating SCD"

    Article Title: CCDC137 knockdown suppresses bladder cancer progression by downregulating SCD

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-025-07033-w

    Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in T24-shCtrl and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance
    Figure Legend Snippet: Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in T24-shCtrl and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Techniques Used: RNA Sequencing, Functional Assay, Sequencing, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay

    In vivo validation of CCDC137 regulating tumor growth. A Subcutaneous xenograft models were established in nude mice to validate the regulatory role of CCDC137 in tumor growth. B Tumor volume growth curves were plotted over 28 days after tumor cell inoculation. C Tumor weights of T24-shCtrl and T24-shCCDC137-1 groups were measured on day 28. D The Graphical Abstract described the key results in this study. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance
    Figure Legend Snippet: In vivo validation of CCDC137 regulating tumor growth. A Subcutaneous xenograft models were established in nude mice to validate the regulatory role of CCDC137 in tumor growth. B Tumor volume growth curves were plotted over 28 days after tumor cell inoculation. C Tumor weights of T24-shCtrl and T24-shCCDC137-1 groups were measured on day 28. D The Graphical Abstract described the key results in this study. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Techniques Used: In Vivo, Biomarker Discovery

    Related Articles

    Gene Knockout:

    Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
    Article Snippet: The survival differences in panel F were evaluated using Advanced Science, 2026 rules of use; w n p d 4 S w s r t U w g 4 T w d g L 4 I l w ( s w T n f p 4 S s s T u g 4 U I w b p c u s A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202510542 by N at Prov Indonesia, on [14/05/2026] .. All lentiviral particles (including those for gene knockout and UGDH variant overexpression) were customized by GENECHEM (Shanghai, China). ..

    Variant Assay:

    Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
    Article Snippet: The survival differences in panel F were evaluated using Advanced Science, 2026 rules of use; w n p d 4 S w s r t U w g 4 T w d g L 4 I l w ( s w T n f p 4 S s s T u g 4 U I w b p c u s A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202510542 by N at Prov Indonesia, on [14/05/2026] .. All lentiviral particles (including those for gene knockout and UGDH variant overexpression) were customized by GENECHEM (Shanghai, China). ..

    Over Expression:

    Article Title: Targeting UXS1-Dependent Glucuronate Detoxification Potentiates Metformin's Anti-Tumor Efficacy in Lung Adenocarcinoma.
    Article Snippet: The survival differences in panel F were evaluated using Advanced Science, 2026 rules of use; w n p d 4 S w s r t U w g 4 T w d g L 4 I l w ( s w T n f p 4 S s s T u g 4 U I w b p c u s A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202510542 by N at Prov Indonesia, on [14/05/2026] .. All lentiviral particles (including those for gene knockout and UGDH variant overexpression) were customized by GENECHEM (Shanghai, China). ..

    Article Title: YTHDF3 Enhances Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporosis by Promoting TBX19 Expression.
    Article Snippet: .. Ythdf3 overexpression (Ythdf3- OE) and control (Ythdf3- NC) lentiviruses were constructed by Genechem. ..

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). ..

    shRNA:

    Article Title: Rhubarb attenuates intracerebral hemorrhage injury by upregulating Snord17 to inhibit PANoptosis.
    Article Snippet: Background: Intracerebral hemorrhage (ICH) is the most severe subtype of stroke and currently lacks effective therapies.. PANoptosis, a novel programmed cell death mode, plays an important role in ICH.. Rhubarb has shown neuroprotective potential in ICH, but its mechanisms remain unclear.

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). ..

    Transfection:

    Article Title: Identification of PRRG1 as a possible molecular target of pancreatic cancer.
    Article Snippet: AR TI CL E IN P RE SS The lentiviral particles encoding PRRG1 (PRRG1 for human) cDNA (“OE-PRRG1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into CFPAC-1 and PATU-8988T cells. .. The lentiviral particles encoding Prrg1 (Prrg1 for mouse) cDNA (“OE-Prrg1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into PANC02 cells. ..

    Article Title: Identification of PRRG1 as a possible molecular target of pancreatic cancer.
    Article Snippet: .. AR TI CL E IN P RE SS The lentiviral particles encoding PRRG1 (PRRG1 for human) cDNA (“OE-PRRG1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into CFPAC-1 and PATU-8988T cells. .. The lentiviral particles encoding Prrg1 (Prrg1 for mouse) cDNA (“OE-Prrg1”) and the empty vector (“Vector”), provided by Genechem (Shanghai, China), were transfected into PANC02 cells.

    Control:

    Article Title: YTHDF3 Enhances Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporosis by Promoting TBX19 Expression.
    Article Snippet: .. Ythdf3 overexpression (Ythdf3- OE) and control (Ythdf3- NC) lentiviruses were constructed by Genechem. ..

    Construct:

    Article Title: YTHDF3 Enhances Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Osteoporosis by Promoting TBX19 Expression.
    Article Snippet: .. Ythdf3 overexpression (Ythdf3- OE) and control (Ythdf3- NC) lentiviruses were constructed by Genechem. ..

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    CRISPR:

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    Sequencing:

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    Infection:

    Article Title: PEBP4 alleviates muscle wasting in lung cancer cachexia via KEAP1-NRF2-mediated redox homeostasis.
    Article Snippet: All siRNAs were synthesized by JTSBIO Co., Ltd., Wuhan, China. .. Lentiviral particles encoding PEBP4 (GeneChem, Shanghai, China) or CRISPR/Cas9 constructs targeting KEAP1 (Focus Biology, Shanghai, China), with the sgRNA sequence 5′-GGACTTTCGTAGCCCCCATG-3′, were used to infect C2C12 cells at a multiplicity of infection (MOI) of 30, according to the manufacturers’ instructions. .. Total RNA was isolated from C2C12 myoblasts and skeletal muscle tissues using the MagZol Reagent (Magen, R4801), following the manufacturer’s protocol. cDNA was synthesized from 1 μg of total RNA using the HiScript II Q RT SuperMix (Vazyme, R223-01).

    Expressing:

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). ..

    Knockdown:

    Article Title: Integrative multi-omics analysis identifies key ubiquitination regulators in prostate cancer
    Article Snippet: .. Lentiviral particles expressing TRIM8 (LV-TRIM8, titer: 6.2 × 108 TU/mL), TRIM8-specific short hairpin RNA (LV-TRIM8-RNAi, titer: 7.9 × 108 TU/mL), and their corresponding negative controls (CON335 for overexpression, titer: 9.9 × 108 TU/mL; CON313 for knockdown, titer: 1.0 × 109 TU/mL) were purchased from GeneChem (Shanghai, China). ..



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    Image Search Results


    ASNS enhanced H 2 O 2 ‐induced retinal cell proliferation and attenuated senescence. ARPE‐19 cells were transfected with shASNS or ASNS‐OE. (A) The mRNA expression of ASNS was detected by RT‐qPCR. (B) The protein expression of ASNS was detected by WB. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (C) CCK8 assay was utilized to assess cell viability in H 2 O 2 ‐treated ARPE‐19 cells. (D) SA‐β‐gal staining experiments were conducted in the above groups. (E) Measurement of intracellular ROS in the above groups. (F) The protein expression of P53, P21, P16 was detected by WB. (G) The level of GSH and MDA detected by ELISA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: ASNS enhanced H 2 O 2 ‐induced retinal cell proliferation and attenuated senescence. ARPE‐19 cells were transfected with shASNS or ASNS‐OE. (A) The mRNA expression of ASNS was detected by RT‐qPCR. (B) The protein expression of ASNS was detected by WB. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (C) CCK8 assay was utilized to assess cell viability in H 2 O 2 ‐treated ARPE‐19 cells. (D) SA‐β‐gal staining experiments were conducted in the above groups. (E) Measurement of intracellular ROS in the above groups. (F) The protein expression of P53, P21, P16 was detected by WB. (G) The level of GSH and MDA detected by ELISA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Staining, Enzyme-linked Immunosorbent Assay

    ASNS regulated glucose metabolism pathways in H 2 O 2 ‐induced retinal cells. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (A) Detection of glucose uptake using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (B) Detection of lactate production using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (C) Measuring ECAR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (D) Measuring OCR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (E) The mRNA expression of Glut1, Glut4, HK2, and PGK1was detected by RT‐qPCR. (F) The protein expression of Glut1, Glut4, HK2, and PGK1was detected by WB. (G) The protein expression of HIF‐1α was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: ASNS regulated glucose metabolism pathways in H 2 O 2 ‐induced retinal cells. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (A) Detection of glucose uptake using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (B) Detection of lactate production using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (C) Measuring ECAR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (D) Measuring OCR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (E) The mRNA expression of Glut1, Glut4, HK2, and PGK1was detected by RT‐qPCR. (F) The protein expression of Glut1, Glut4, HK2, and PGK1was detected by WB. (G) The protein expression of HIF‐1α was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Expressing, Quantitative RT-PCR

    Validation of ASNS's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weight) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shASNS (Injection of shASNS lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of ASNS and USP13 in retinal tissues was detected by WB. (G) IF was used to analyze the expression of ASNS and USP1 in retinal tissues. (H) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: Validation of ASNS's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weight) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shASNS (Injection of shASNS lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of ASNS and USP13 in retinal tissues was detected by WB. (G) IF was used to analyze the expression of ASNS and USP1 in retinal tissues. (H) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Biomarker Discovery, In Vivo, Injection, Staining, Expressing, Quantitative RT-PCR

    Validation of USP13's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weigh) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shUSP13 (Injection of shUSP13 lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of USP13 in retinal tissues was detected by WB. (G) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: Validation of USP13's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weigh) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shUSP13 (Injection of shUSP13 lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of USP13 in retinal tissues was detected by WB. (G) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Biomarker Discovery, In Vivo, Injection, Staining, Expressing, Quantitative RT-PCR

    Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in T24-shCtrl and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Journal: Journal of Translational Medicine

    Article Title: CCDC137 knockdown suppresses bladder cancer progression by downregulating SCD

    doi: 10.1186/s12967-025-07033-w

    Figure Lengend Snippet: Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in T24-shCtrl and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Article Snippet: The shCCDC137 lentiviral particles and corresponding control lentiviral particles (shCtrl) were purchased from Genechem Co., Ltd (Shanghai, China).

    Techniques: RNA Sequencing, Functional Assay, Sequencing, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay

    In vivo validation of CCDC137 regulating tumor growth. A Subcutaneous xenograft models were established in nude mice to validate the regulatory role of CCDC137 in tumor growth. B Tumor volume growth curves were plotted over 28 days after tumor cell inoculation. C Tumor weights of T24-shCtrl and T24-shCCDC137-1 groups were measured on day 28. D The Graphical Abstract described the key results in this study. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Journal: Journal of Translational Medicine

    Article Title: CCDC137 knockdown suppresses bladder cancer progression by downregulating SCD

    doi: 10.1186/s12967-025-07033-w

    Figure Lengend Snippet: In vivo validation of CCDC137 regulating tumor growth. A Subcutaneous xenograft models were established in nude mice to validate the regulatory role of CCDC137 in tumor growth. B Tumor volume growth curves were plotted over 28 days after tumor cell inoculation. C Tumor weights of T24-shCtrl and T24-shCCDC137-1 groups were measured on day 28. D The Graphical Abstract described the key results in this study. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Article Snippet: The shCCDC137 lentiviral particles and corresponding control lentiviral particles (shCtrl) were purchased from Genechem Co., Ltd (Shanghai, China).

    Techniques: In Vivo, Biomarker Discovery