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lentiviral vector plko 1 shrna control shctrl  (Addgene inc)


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    Addgene inc lentiviral vector plko 1 shrna control shctrl
    Lentiviral Vector Plko 1 Shrna Control Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+shctrl/pLKO%2E1+-+TRC+control+(Plasmid+%2310879)/pm41596422-229-0-6
    Average 96 stars, based on 376 article reviews
    lentiviral vector plko 1 shrna control shctrl - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Fatty acids promote uncoupled respiration via ATP/ADP carriers in white adipocytes
    Article Snippet: .. The following reagents were used in this study: amphotericin B (Sigma catalogue number A2411), antimycin A (Sigma catalogue number A8674), atglistatin (Sigma catalogue number SML1075), BKA (Enzo catalogue number BML-CM113), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Sigma catalogue number C2920), CL-316,243 (Sigma catalogue number C5979), collagenase (Sigma catalogue number C6885), CSA (Enzo catalogue number BML-A195), dexamethasone (Sigma catalogue number D4902), DMEM/F-12 50/50 (Corning catalogue number 15-090), etomoxir (AmBeed catalogue number A108121), fetal bovine serum (FBS, Corning catalogue number 35-010), extracellular matrix gel from Engelbreth–Holm–Swarm murine sarcoma (Sigma catalogue number E1270), fibronectin (Sigma catalogue number F1141) forskolin (Sigma catalogue number F3917), FS high-glucose DMEM (Cytiva SH30243), hexadimethrine bromide (polybrene) (Sigma catalogue number H9268), insulin (Sigma catalogue number I6634), 3-isobutyl-1-methylxanthine (Sigma catalogue number I5879), isoproterenol (Sigma catalogue number PHR2722), JC-10 (AAT Bioquest catalogue number 22204), Lenti-X Packaging Single Shots (Takara catalogue number 631276), Maxiprep Kit (Qiagen catalogue number 12162), Miniprep Kit (Qiagen catalogue number 27106), MitoTracker Green (Fisher catalogue number M7514), norepinephrine (Sigma catalogue number A7257), Oligomycin A (Sigma catalogue number 75351), Pen Strep Glutamine (Gibco catalogue number 10378-016), pentobarbital (Covetrus catalogue number 081799), PF-04620110 (DGATi, Sigma catalogue number PZ0207), pLKO.1 Backbone (Addgene Plasmid catalogue number 10879), poly lysine (Sigma catalogue number P1274), Puradisc sterile PES syringe filters (Cytiva catalogue number 6780-1304), puromycin (Sigma catalogue number P8833), rosiglitazone (Sigma catalogue number 557366), rotenone (Sigma catalogue number R8875), Stbl3 Competent E. Coli (Thermo Fisher catalogue number C737303), Tet approved FBS (Takara catalogue number 631107), TMRM (Fisher catalogue number I34361 ). ..

    Article Title: Fatty acids promote uncoupled respiration via ATP/ADP carriers in white adipocytes.
    Article Snippet: .. The following reagents were used in this study: amphotericin B (Sigma catalogue number A2411), antimycin A (Sigma catalogue number A8674), atglistatin (Sigma catalogue number SML1075), BKA (Enzo catalogue number BML-CM113), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, Sigma catalogue number C2920), CL-316,243 (Sigma catalogue number C5979), collagenase (Sigma catalogue number C6885), CSA (Enzo catalogue number BML-A195), dexamethasone (Sigma catalogue number D4902), DMEM/F-12 50/50 (Corning catalogue number 15-090), etomoxir (AmBeed catalogue number A108121), fetal bovine serum (FBS, Corning catalogue number 35-010), extracellular matrix gel from Engelbreth–Holm–Swarm murine sarcoma (Sigma catalogue number E1270), fibronectin (Sigma catalogue number F1141) forskolin (Sigma catalogue number F3917), FS high-glucose DMEM (Cytiva SH30243), hexadimethrine bromide (polybrene) (Sigma catalogue number H9268), insulin (Sigma catalogue number I6634), 3-isobutyl-1-methylxanthine (Sigma catalogue number I5879), isoproterenol (Sigma catalogue number PHR2722), JC-10 (AAT Bioquest catalogue number 22204), Lenti-X Packaging Single Shots (Takara catalogue number 631276), Maxiprep Kit (Qiagen catalogue number 12162), Miniprep Kit (Qiagen catalogue number 27106), MitoTracker Green (Fisher catalogue number M7514), norepinephrine (Sigma catalogue number A7257), Oligomycin A (Sigma catalogue number 75351), Pen Strep Glutamine (Gibco catalogue number 10378-016), pentobarbital (Covetrus catalogue number 081799), PF-04620110 (DGATi, Sigma catalogue number PZ0207), pLKO.1 Backbone (Addgene Plasmid catalogue number 10879), poly lysine (Sigma catalogue number P1274), Puradisc sterile PES syringe filters (Cytiva catalogue number 6780- 1304), puromycin (Sigma catalogue number P8833), rosiglitazone (Sigma catalogue number 557366), rotenone (Sigma catalogue number R8875), Stbl3 Competent E. Coli (Thermo Fisher catalogue number C737303), Tet approved FBS (Takara catalogue number 631107), TMRM (Fisher catalogue number I34361). ..

    Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells.
    Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Lentiviral vector pLKO.1 shRNA control (shCtrl) (Addgene Plasmid #10879) was purchased from Addgene; pLKO-puro shNRF2 (NM_010902) was purchased from Sigma-Aldrich. ..

    Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells
    Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Lentiviral vector pLKO.1 shRNA control (shCtrl) (Addgene Plasmid #10879) was purchased from Addgene; pLKO-puro shNRF2 (NM_010902) was purchased from Sigma-Aldrich. ..

    Sterility:

    Article Title: Fatty acids promote uncoupled respiration via ATP/ADP carriers in white adipocytes
    Article Snippet: .. The following reagents were used in this study: amphotericin B (Sigma catalogue number A2411), antimycin A (Sigma catalogue number A8674), atglistatin (Sigma catalogue number SML1075), BKA (Enzo catalogue number BML-CM113), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Sigma catalogue number C2920), CL-316,243 (Sigma catalogue number C5979), collagenase (Sigma catalogue number C6885), CSA (Enzo catalogue number BML-A195), dexamethasone (Sigma catalogue number D4902), DMEM/F-12 50/50 (Corning catalogue number 15-090), etomoxir (AmBeed catalogue number A108121), fetal bovine serum (FBS, Corning catalogue number 35-010), extracellular matrix gel from Engelbreth–Holm–Swarm murine sarcoma (Sigma catalogue number E1270), fibronectin (Sigma catalogue number F1141) forskolin (Sigma catalogue number F3917), FS high-glucose DMEM (Cytiva SH30243), hexadimethrine bromide (polybrene) (Sigma catalogue number H9268), insulin (Sigma catalogue number I6634), 3-isobutyl-1-methylxanthine (Sigma catalogue number I5879), isoproterenol (Sigma catalogue number PHR2722), JC-10 (AAT Bioquest catalogue number 22204), Lenti-X Packaging Single Shots (Takara catalogue number 631276), Maxiprep Kit (Qiagen catalogue number 12162), Miniprep Kit (Qiagen catalogue number 27106), MitoTracker Green (Fisher catalogue number M7514), norepinephrine (Sigma catalogue number A7257), Oligomycin A (Sigma catalogue number 75351), Pen Strep Glutamine (Gibco catalogue number 10378-016), pentobarbital (Covetrus catalogue number 081799), PF-04620110 (DGATi, Sigma catalogue number PZ0207), pLKO.1 Backbone (Addgene Plasmid catalogue number 10879), poly lysine (Sigma catalogue number P1274), Puradisc sterile PES syringe filters (Cytiva catalogue number 6780-1304), puromycin (Sigma catalogue number P8833), rosiglitazone (Sigma catalogue number 557366), rotenone (Sigma catalogue number R8875), Stbl3 Competent E. Coli (Thermo Fisher catalogue number C737303), Tet approved FBS (Takara catalogue number 631107), TMRM (Fisher catalogue number I34361 ). ..

    Article Title: Fatty acids promote uncoupled respiration via ATP/ADP carriers in white adipocytes.
    Article Snippet: .. The following reagents were used in this study: amphotericin B (Sigma catalogue number A2411), antimycin A (Sigma catalogue number A8674), atglistatin (Sigma catalogue number SML1075), BKA (Enzo catalogue number BML-CM113), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, Sigma catalogue number C2920), CL-316,243 (Sigma catalogue number C5979), collagenase (Sigma catalogue number C6885), CSA (Enzo catalogue number BML-A195), dexamethasone (Sigma catalogue number D4902), DMEM/F-12 50/50 (Corning catalogue number 15-090), etomoxir (AmBeed catalogue number A108121), fetal bovine serum (FBS, Corning catalogue number 35-010), extracellular matrix gel from Engelbreth–Holm–Swarm murine sarcoma (Sigma catalogue number E1270), fibronectin (Sigma catalogue number F1141) forskolin (Sigma catalogue number F3917), FS high-glucose DMEM (Cytiva SH30243), hexadimethrine bromide (polybrene) (Sigma catalogue number H9268), insulin (Sigma catalogue number I6634), 3-isobutyl-1-methylxanthine (Sigma catalogue number I5879), isoproterenol (Sigma catalogue number PHR2722), JC-10 (AAT Bioquest catalogue number 22204), Lenti-X Packaging Single Shots (Takara catalogue number 631276), Maxiprep Kit (Qiagen catalogue number 12162), Miniprep Kit (Qiagen catalogue number 27106), MitoTracker Green (Fisher catalogue number M7514), norepinephrine (Sigma catalogue number A7257), Oligomycin A (Sigma catalogue number 75351), Pen Strep Glutamine (Gibco catalogue number 10378-016), pentobarbital (Covetrus catalogue number 081799), PF-04620110 (DGATi, Sigma catalogue number PZ0207), pLKO.1 Backbone (Addgene Plasmid catalogue number 10879), poly lysine (Sigma catalogue number P1274), Puradisc sterile PES syringe filters (Cytiva catalogue number 6780- 1304), puromycin (Sigma catalogue number P8833), rosiglitazone (Sigma catalogue number 557366), rotenone (Sigma catalogue number R8875), Stbl3 Competent E. Coli (Thermo Fisher catalogue number C737303), Tet approved FBS (Takara catalogue number 631107), TMRM (Fisher catalogue number I34361). ..

    other:

    Article Title: DCPS modulates TDP-43-linked neurodegeneration through P-body-mediated RNA decay
    Article Snippet: Please refer to Table S5 for more details N/A ARTR-seq adapter-RT primer(5 ′ -AGACGTGTG CTCTTCCGATCTNNNNNNNNNN-3 ′ ) Xiao et al. 60 N/A ARTR-seq 3 ′ cDNA adapter (5 ′ Phos-8N-AGAT CGGAAGAGGTCGTGT-3 ′ SpC3) Xiao et al. 60 N/A Recombinant DNA Plasmid: pMDLg/pRRE Dull et al. 81 RRID: Addgene_12251 Plasmid: pRSV-Rev Dull et al. 81 RRID: Addgene_12253 Plasmid: pMD2.G A gift from Didier Trono RRID: Addgene_12259 Plasmid: pLG1-control sgRNA Tian et al. 41 N/A Plasmid: pLG1-TDP-43 sgRNA This paper N/A Plasmid: pLG1-DCPS sgRNA 1 This paper N/A Plasmid: pLG1-DCPS sgRNA 2 This paper N/A Plasmid: pLKO.1-control shRNA A gift from David Root 82 RRID: Addgene_10879 Plasmid: pLKO.1-TDP-43 shRNA This paper N/A Plasmid: pLKO.1-DCPS shRNA #1 This paper N/A Plasmid: pLKO.1-DCPS shRNA #2 This paper N/A Plasmid: Lenti-GFP-puro Zhang et al. 45 N/A Plasmid: Lenti-FLAG-TDP-43 dNLS This paper N/A Plasmid: Lenti- FLAG-TDP-43 WT -APEX2 This paper N/A Plasmid: Lenti- FLAG-TDP-43 dNLS -APEX2 This paper N/A Plasmid: pEGFP-N1-TDP-43 Cheng et al. 83 N/A Plasmid: pcDNA3.1-FLAG-TDP-43 dNLS This paper N/A Plasmid: pT7-EGFP-C1-HsDCP2 Tritschler et al. 84 RRID: Addgene_25031 (Continued on next page) e4 Neuron 114, 1–16.e1–e12, June 3, 2026

    shRNA:

    Article Title: Downregulation of TDP43 by atovaquone inhibits oxidative phosphorylation and enhances sensitivity of triple-negative breast cancer to EGFR-TKIs.
    Article Snippet: .. We used sh-TRC (Addgene #10879) as the shRNA control, 239 nontargeting siRNA as the siRNA control. ..

    Article Title: Deuterium modification of tyrosine kinase inhibitors contributes to reversing ferroptosis resistance through upregulation of aldehyde oxidase 1 in hepatocellular carcinoma
    Article Snippet: .. For RNA interference, shRNA sequences against AOX1 and ACSL5 were obtained from Sigma–Aldrich and cloned into pLKO.1 TRC (#10879; Addgene, Cambridge, MA, USA). ..

    Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells.
    Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Lentiviral vector pLKO.1 shRNA control (shCtrl) (Addgene Plasmid #10879) was purchased from Addgene; pLKO-puro shNRF2 (NM_010902) was purchased from Sigma-Aldrich. ..

    Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells
    Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Lentiviral vector pLKO.1 shRNA control (shCtrl) (Addgene Plasmid #10879) was purchased from Addgene; pLKO-puro shNRF2 (NM_010902) was purchased from Sigma-Aldrich. ..

    Control:

    Article Title: Downregulation of TDP43 by atovaquone inhibits oxidative phosphorylation and enhances sensitivity of triple-negative breast cancer to EGFR-TKIs.
    Article Snippet: .. We used sh-TRC (Addgene #10879) as the shRNA control, 239 nontargeting siRNA as the siRNA control. ..

    Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells.
    Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Lentiviral vector pLKO.1 shRNA control (shCtrl) (Addgene Plasmid #10879) was purchased from Addgene; pLKO-puro shNRF2 (NM_010902) was purchased from Sigma-Aldrich. ..

    Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells
    Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Lentiviral vector pLKO.1 shRNA control (shCtrl) (Addgene Plasmid #10879) was purchased from Addgene; pLKO-puro shNRF2 (NM_010902) was purchased from Sigma-Aldrich. ..

    Clone Assay:

    Article Title: Deuterium modification of tyrosine kinase inhibitors contributes to reversing ferroptosis resistance through upregulation of aldehyde oxidase 1 in hepatocellular carcinoma
    Article Snippet: .. For RNA interference, shRNA sequences against AOX1 and ACSL5 were obtained from Sigma–Aldrich and cloned into pLKO.1 TRC (#10879; Addgene, Cambridge, MA, USA). ..



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    Image Search Results


    ASNS enhanced H 2 O 2 ‐induced retinal cell proliferation and attenuated senescence. ARPE‐19 cells were transfected with shASNS or ASNS‐OE. (A) The mRNA expression of ASNS was detected by RT‐qPCR. (B) The protein expression of ASNS was detected by WB. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (C) CCK8 assay was utilized to assess cell viability in H 2 O 2 ‐treated ARPE‐19 cells. (D) SA‐β‐gal staining experiments were conducted in the above groups. (E) Measurement of intracellular ROS in the above groups. (F) The protein expression of P53, P21, P16 was detected by WB. (G) The level of GSH and MDA detected by ELISA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: ASNS enhanced H 2 O 2 ‐induced retinal cell proliferation and attenuated senescence. ARPE‐19 cells were transfected with shASNS or ASNS‐OE. (A) The mRNA expression of ASNS was detected by RT‐qPCR. (B) The protein expression of ASNS was detected by WB. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (C) CCK8 assay was utilized to assess cell viability in H 2 O 2 ‐treated ARPE‐19 cells. (D) SA‐β‐gal staining experiments were conducted in the above groups. (E) Measurement of intracellular ROS in the above groups. (F) The protein expression of P53, P21, P16 was detected by WB. (G) The level of GSH and MDA detected by ELISA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Staining, Enzyme-linked Immunosorbent Assay

    ASNS regulated glucose metabolism pathways in H 2 O 2 ‐induced retinal cells. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (A) Detection of glucose uptake using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (B) Detection of lactate production using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (C) Measuring ECAR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (D) Measuring OCR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (E) The mRNA expression of Glut1, Glut4, HK2, and PGK1was detected by RT‐qPCR. (F) The protein expression of Glut1, Glut4, HK2, and PGK1was detected by WB. (G) The protein expression of HIF‐1α was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: ASNS regulated glucose metabolism pathways in H 2 O 2 ‐induced retinal cells. ARPE‐19 cells were divided into H 2 O 2 + shCtrl, H 2 O 2 + shASNS or H 2 O 2 + NC, H 2 O 2 + ASNS‐OE groups. (A) Detection of glucose uptake using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (B) Detection of lactate production using a kit in H 2 O 2 ‐treated ARPE‐19 cells. (C) Measuring ECAR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (D) Measuring OCR in H 2 O 2 ‐treated ARPE‐19 cells using the XF‐96 extracellular flux analyzer. (E) The mRNA expression of Glut1, Glut4, HK2, and PGK1was detected by RT‐qPCR. (F) The protein expression of Glut1, Glut4, HK2, and PGK1was detected by WB. (G) The protein expression of HIF‐1α was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Expressing, Quantitative RT-PCR

    Validation of ASNS's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weight) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shASNS (Injection of shASNS lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of ASNS and USP13 in retinal tissues was detected by WB. (G) IF was used to analyze the expression of ASNS and USP1 in retinal tissues. (H) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: Validation of ASNS's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weight) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shASNS (Injection of shASNS lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of ASNS and USP13 in retinal tissues was detected by WB. (G) IF was used to analyze the expression of ASNS and USP1 in retinal tissues. (H) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Biomarker Discovery, In Vivo, Injection, Staining, Expressing, Quantitative RT-PCR

    Validation of USP13's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weigh) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shUSP13 (Injection of shUSP13 lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of USP13 in retinal tissues was detected by WB. (G) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biofactors (Oxford, England)

    Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

    doi: 10.1002/biof.70057

    Figure Lengend Snippet: Validation of USP13's impact on the AMD disease process in vivo. Sprague–Dawley rats injected with sodium iodate (30 mg/kg body weigh) and divided into MOCK group (No lentivirus injections, n = 10), shCtrl group (Injection of shCtrl lentivirus, n = 10), and shUSP13 (Injection of shUSP13 lentivirus, n = 10) group. (A) H&E staining analysis of rat retinal tissues in each group. (B) SA‐β‐gal staining of rat retinal tissues were conducted in the above groups. (C) Measuring ECAR and OCR in retinal tissues using the XF‐96 extracellular flux analyzer. (D) The mRNA expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by RT‐qPCR. (E) The protein expression of Glut1, Glut4, HK2, and PGK1 in retinal tissues was detected by WB. (F) The protein expression of USP13 in retinal tissues was detected by WB. (G) The protein expression of HIF‐1α in retinal tissues was detected by WB. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Lentiviral vectors interfering with ASNS and USP13 expression (shASNS and shUSP13) and the negative control shCtrl were synthesized by GeneChem (Shanghai, China).

    Techniques: Biomarker Discovery, In Vivo, Injection, Staining, Expressing, Quantitative RT-PCR

    Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in T24-shCtrl and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Journal: Journal of Translational Medicine

    Article Title: CCDC137 knockdown suppresses bladder cancer progression by downregulating SCD

    doi: 10.1186/s12967-025-07033-w

    Figure Lengend Snippet: Exploration of potential downstream mechanism and upstream regulators of CCDC137. A Heatmap shows DEGs identified by RNA sequencing in T24-shCtrl and T24-shCCDC137-1 cells. B Bar plot displays GO and KEGG functional enrichment results of downregulated DEGs from RNA sequencing. C GSVA-Hallmark pathway enrichment analysis displayed differences between CCDC137 positive (CCDC137 +) and CCDC137 negative (CCDC137 −) cells based on single-cell sequencing data. D mRNA and protein expression of stearoyl-CoA desaturase (SCD) were detected by qRT-PCR and Western blot. E DecoupleR was used to analyze differences in transcription factor activity between CCDC137 + and CCDC137 − epithelial cells in single-cell sequencing data. F TF-Target Finder was employed to identify potential upstream transcription factors of CCDC137. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Article Snippet: The shCCDC137 lentiviral particles and corresponding control lentiviral particles (shCtrl) were purchased from Genechem Co., Ltd (Shanghai, China).

    Techniques: RNA Sequencing, Functional Assay, Sequencing, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay

    In vivo validation of CCDC137 regulating tumor growth. A Subcutaneous xenograft models were established in nude mice to validate the regulatory role of CCDC137 in tumor growth. B Tumor volume growth curves were plotted over 28 days after tumor cell inoculation. C Tumor weights of T24-shCtrl and T24-shCCDC137-1 groups were measured on day 28. D The Graphical Abstract described the key results in this study. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Journal: Journal of Translational Medicine

    Article Title: CCDC137 knockdown suppresses bladder cancer progression by downregulating SCD

    doi: 10.1186/s12967-025-07033-w

    Figure Lengend Snippet: In vivo validation of CCDC137 regulating tumor growth. A Subcutaneous xenograft models were established in nude mice to validate the regulatory role of CCDC137 in tumor growth. B Tumor volume growth curves were plotted over 28 days after tumor cell inoculation. C Tumor weights of T24-shCtrl and T24-shCCDC137-1 groups were measured on day 28. D The Graphical Abstract described the key results in this study. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, no statistical significance

    Article Snippet: The shCCDC137 lentiviral particles and corresponding control lentiviral particles (shCtrl) were purchased from Genechem Co., Ltd (Shanghai, China).

    Techniques: In Vivo, Biomarker Discovery