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Promega control cdna sample
Control Cdna Sample, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Narcissus yellow stripe virus and Narcissus mosaic virus detection in Narcissus via multiplex TaqMan-based reverse transcription-PCR assay.
Article Snippet: Aims: Development of a multiplex TaqMan RT-qPCR assay to simultaneously detect Narcissus yellow stripe virus (NYSV) and Narcissus mosaic virus (NMV), frequently causing mixed narcissus infection.. Feasibility verification was confirmed in natural samples.. Methods and Results: Primers and probes were designed based on the conserved CP gene regions of NYSV or NMV and their suitability for singleplex and multiplex TaqMan RT-qPCR assays as well as for conventional RT-PCR.

Article Title: Quorum sensing and RsaM regulons of the rice pathogen Pseudomonas fuscovaginae .
Article Snippet: The synthesized cDNA samples were diluted to 25 ng μl 1, and 2 μl cDNA was mixed with GoTaq qPCR Master Mix kit (Promega), containing SYBR green, and specific primers in a final volume of 12 μl.

Article Title: Identification of a Virulent Newcastle Disease Virus Strain Isolated from Pigeons ( Columbia livia ) in Northeastern Brazil Using Next-Generation Genome Sequencing
Article Snippet: Next, cDNA samples were amplified employing the kit GoTaq ® Probe qPCR (Promega Corporation, Madison, WI, USA) in a QuantStudio5 ® Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA).

Mutagenesis:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Transfection:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Polymerase Chain Reaction:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Article Title: Integrative Multiparametric Analysis of Circulating Cell-Free Nucleic Acids of Plasma in Healthy Individuals During Aging.
Article Snippet: .. A control human genomic DNA (Promega) and a control cDNA sample were added to PCR plates with ccfDNA and ccfRNA assays, respectively. ..

Sequencing:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Amplification:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Synthesized:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Isolation:

Article Title: Multi-Omic Analysis of Two Common P53 Mutations: Proteins Regulated by Mutated P53 as Potential Targets for Immunotherapy
Article Snippet: .. TP53 mutation status was confirmed in the transfected cell lines using PCR and Sanger sequencing of products amplified using primers flanking the TP53 mutations (forward primer 5′ TCTTCTGTCCCTTCCCAGAA3′ and reverse primer 5′ CAAGGCCTCATTCAGCTCTC3′) and cDNA template synthesized (Promega, Madison, WI, USA) using RNA isolated (Qiagen, Hilden, Germany) from the transfected cell lines. .. Cell adhesion assays were performed using Collagen I-coated plates (Gibco).

Quantitative RT-PCR:

Article Title: Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP
Article Snippet: One microgram of RNA was transcribed into cDNA using random primers and Moloney murine leukemia virus reverse transcriptase (Promega, Charbonnieres, France). .. RT-qPCR was performed using the resulting cDNA templates and GoTaq qPCR master mix (Promega) with an Applied Biosystems 7300 real-time PCR cycler (see the supplemental material for the primer sequences). .. The PCR data were analyzed using SDS software (Applied Biosystems), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) expression was used as an internal control.

Real-time Polymerase Chain Reaction:

Article Title: Placental Alkaline Phosphatase Promotes Zika Virus Replication by Stabilizing Viral Proteins through BIP
Article Snippet: One microgram of RNA was transcribed into cDNA using random primers and Moloney murine leukemia virus reverse transcriptase (Promega, Charbonnieres, France). .. RT-qPCR was performed using the resulting cDNA templates and GoTaq qPCR master mix (Promega) with an Applied Biosystems 7300 real-time PCR cycler (see the supplemental material for the primer sequences). .. The PCR data were analyzed using SDS software (Applied Biosystems), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) expression was used as an internal control.

Control:

Article Title: Integrative Multiparametric Analysis of Circulating Cell-Free Nucleic Acids of Plasma in Healthy Individuals During Aging.
Article Snippet: .. A control human genomic DNA (Promega) and a control cDNA sample were added to PCR plates with ccfDNA and ccfRNA assays, respectively. ..



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Image Search Results


(A) qRT-PCR analysis (in triplicate) of samples from patients with BL (n = 15) compared with normal lymphoid control tissue (n = 7). mRNA levels were normalized to β-actin and presented as 2–ΔΔCt. Bars represent the mean ± SEM. *P = 9.1 × 10–4, by t test. (B) Microarray gene expression–profiling data for BCL-W and BCL-2 mRNA in BL (n = 57) compared with normal B cells (n = 49). Bars represent the mean ± SEM. Each circle represents 1 sample, and the y axis represents normalized expression of BCL-W and BCL-2. *P = 1.8 × 10–8, by t test. The data sets analyzed are listed in Supplemental Table 1. (C) Immunohistochemical analysis for BCL-W and BCL-2 protein was performed on samples from patients with BL (n = 26). Box and whisker plots of pathologist scores and representative images of BCL-W and BCL-2 staining from the same tumor sample are shown. Original magnification, ×40; scale bars: 200 μm. *P < 0.0001, by t test. For the box and whisker plots, the box represents the 25th and 75th percentiles, the line indicates the median, the circle indicates the mean, and the whiskers represent the maximum and minimum. (D) Protein expression was assessed by Western blotting for the indicated proteins in BL and DLBCL cell lines. Controls included tissue from 2 human spleens and purified B cells from human peripheral blood.

Journal: The Journal of Clinical Investigation

Article Title: BCL-W has a fundamental role in B cell survival and lymphomagenesis

doi: 10.1172/JCI89486

Figure Lengend Snippet: (A) qRT-PCR analysis (in triplicate) of samples from patients with BL (n = 15) compared with normal lymphoid control tissue (n = 7). mRNA levels were normalized to β-actin and presented as 2–ΔΔCt. Bars represent the mean ± SEM. *P = 9.1 × 10–4, by t test. (B) Microarray gene expression–profiling data for BCL-W and BCL-2 mRNA in BL (n = 57) compared with normal B cells (n = 49). Bars represent the mean ± SEM. Each circle represents 1 sample, and the y axis represents normalized expression of BCL-W and BCL-2. *P = 1.8 × 10–8, by t test. The data sets analyzed are listed in Supplemental Table 1. (C) Immunohistochemical analysis for BCL-W and BCL-2 protein was performed on samples from patients with BL (n = 26). Box and whisker plots of pathologist scores and representative images of BCL-W and BCL-2 staining from the same tumor sample are shown. Original magnification, ×40; scale bars: 200 μm. *P < 0.0001, by t test. For the box and whisker plots, the box represents the 25th and 75th percentiles, the line indicates the median, the circle indicates the mean, and the whiskers represent the maximum and minimum. (D) Protein expression was assessed by Western blotting for the indicated proteins in BL and DLBCL cell lines. Controls included tissue from 2 human spleens and purified B cells from human peripheral blood.

Article Snippet: For qRT-PCR analysis of DLBCL, TissueScan cDNA arrays containing 26 samples from patients with DLBCL and 10 normal lymphoid tissue controls were obtained from OriGene.

Techniques: Quantitative RT-PCR, Microarray, Expressing, Immunohistochemical staining, Whisker Assay, Staining, Western Blot, Purification

(A) Immunohistochemical analysis for BCL-W and BCL-2 protein was performed on samples from patients with DLBCL (n = 57). Box and whisker plots of pathologist scores and representative images of BCL-W and BCL-2 staining from the same 3 tumor samples. Original magnification, ×40; scale bars: 200 μm. For the box and whisker plots, the box represents the 25th and 75th percentiles, the line indicates the median, the circle indicates the mean, and the whiskers represent the maximum and minimum. (B) qRT-PCR analysis in triplicate of samples from patients with DLBCL (n = 26) compared with normal lymphoid control tissue (n = 10). mRNA levels were normalized to β-actin and are presented as 2–ΔΔCt. Error bars represent the mean ± SEM. **P < 6.03 × 10–5 and *P = 0.014, by t test. (C) Microarray gene expression–profiling data for BCL-W and BCL-2 mRNA in DLBCL (n = 319) compared with normal B cells (n = 49). Lines represent the mean ± SEM. Each circle represents 1 sample, and the y axis represents normalized expression of BCL-W and BCL-2. **P < 2.2 × 10–16 and *P = 9.96 × 10–5, by t test. The data sets analyzed are listed in Supplemental Table 1. (D) Patient samples of DLBCL with low BCL-2 from the GSE31312 and GSE10846 data sets were each separated into 2 groups (BCL-W high and BCL-W low) on the basis of the median expression of BCL-W, and Kaplan-Meier analyses were performed. The P values in D were determined by log-rank test.

Journal: The Journal of Clinical Investigation

Article Title: BCL-W has a fundamental role in B cell survival and lymphomagenesis

doi: 10.1172/JCI89486

Figure Lengend Snippet: (A) Immunohistochemical analysis for BCL-W and BCL-2 protein was performed on samples from patients with DLBCL (n = 57). Box and whisker plots of pathologist scores and representative images of BCL-W and BCL-2 staining from the same 3 tumor samples. Original magnification, ×40; scale bars: 200 μm. For the box and whisker plots, the box represents the 25th and 75th percentiles, the line indicates the median, the circle indicates the mean, and the whiskers represent the maximum and minimum. (B) qRT-PCR analysis in triplicate of samples from patients with DLBCL (n = 26) compared with normal lymphoid control tissue (n = 10). mRNA levels were normalized to β-actin and are presented as 2–ΔΔCt. Error bars represent the mean ± SEM. **P < 6.03 × 10–5 and *P = 0.014, by t test. (C) Microarray gene expression–profiling data for BCL-W and BCL-2 mRNA in DLBCL (n = 319) compared with normal B cells (n = 49). Lines represent the mean ± SEM. Each circle represents 1 sample, and the y axis represents normalized expression of BCL-W and BCL-2. **P < 2.2 × 10–16 and *P = 9.96 × 10–5, by t test. The data sets analyzed are listed in Supplemental Table 1. (D) Patient samples of DLBCL with low BCL-2 from the GSE31312 and GSE10846 data sets were each separated into 2 groups (BCL-W high and BCL-W low) on the basis of the median expression of BCL-W, and Kaplan-Meier analyses were performed. The P values in D were determined by log-rank test.

Article Snippet: For qRT-PCR analysis of DLBCL, TissueScan cDNA arrays containing 26 samples from patients with DLBCL and 10 normal lymphoid tissue controls were obtained from OriGene.

Techniques: Immunohistochemical staining, Whisker Assay, Staining, Quantitative RT-PCR, Microarray, Expressing