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gfp aav control construct serotype 2 1  (Addgene inc)


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    Structured Review

    Addgene inc gfp aav control construct serotype 2 1
    Gfp Aav Control Construct Serotype 2 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+aav+construct/AAV+GFP+(Plasmid+%2349055)/pm29150673-213-14-22
    Average 95 stars, based on 64 article reviews
    gfp aav control construct serotype 2 1 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Regulation of Glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons
    Article Snippet: .. All AAV plasmid backbones were based on AAV-GFP.Cre (Addgene, 49056). ..

    Article Title: Locus coeruleus norepinephrine neurons facilitate orbitofrontal cortex remapping and behavioral flexibility.
    Article Snippet: For rabies tracing experiments, AAV8-hSyn-FLEx loxP -TVA-mCherry and AAV8-hSyn-FLEx loxP -H2B-P2A-N2cG-WPRE were mixed at a 1:1 ratio prior to injecting 200 nL into the left OFC of Emx1 Cre (The Jackson Laboratory, 005628) and Gad2 Cre (The Jackson Laboratory, 010802) mice. .. Additional Dbh Cre KI (The Jackson Laboratory, 033951) mice (n = 2) were injected with Cre-dependent AAV encoding GFP (AAV-CAG-FLEx-eGFP, titer: 2.5*10 ̂13; Addgene. ..

    Article Title: GCGR agonism requires GABAergic signaling in the medial basal hypothalamus to promote weight loss in obese mice
    Article Snippet: .. AAV-hSyn-Cre-GFP (3.1 × 10 13 GC/ml), Addgene plasmid 105,540, serotype 9; AAV-CAG-GFP (2.4 × 10 13 GC/ml), Addgene plasmid 37,825, serotype 9. ..

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples.
    Article Snippet: .. After profiling our recombinant AAV samples, we next isolated genomes harboring a GFP expression cassette from an AAV preparation obtained from a commercial vendor (Addgene; pAAV-hSyn-eGFP) and prepared UMI-labeled amplicons for nanopore sequencing. ..

    Article Title: Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytes
    Article Snippet: pAAV-CA-FLEX , Addgene , 38042. .. AAV GFP (plasmid) , Addgene , 49055. .. Plasmid - pDP8.ape , PlasmidFactory , PF0478.

    Article Title: Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons.
    Article Snippet: .. All AAV plasmid backbones were based on AAV-GFP.Cre59 (Addgene, 49056). ..

    Article Title: Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons
    Article Snippet: .. All AAV plasmid backbones were based on AAV-GFP.Cre (Addgene, 49056). ..

    Plasmid Preparation:

    Article Title: Regulation of Glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons
    Article Snippet: .. All AAV plasmid backbones were based on AAV-GFP.Cre (Addgene, 49056). ..

    Article Title: GCGR agonism requires GABAergic signaling in the medial basal hypothalamus to promote weight loss in obese mice
    Article Snippet: .. AAV-hSyn-Cre-GFP (3.1 × 10 13 GC/ml), Addgene plasmid 105,540, serotype 9; AAV-CAG-GFP (2.4 × 10 13 GC/ml), Addgene plasmid 37,825, serotype 9. ..

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Article Title: Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytes
    Article Snippet: pAAV-CA-FLEX , Addgene , 38042. .. AAV GFP (plasmid) , Addgene , 49055. .. Plasmid - pDP8.ape , PlasmidFactory , PF0478.

    Article Title: Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons.
    Article Snippet: .. All AAV plasmid backbones were based on AAV-GFP.Cre59 (Addgene, 49056). ..

    Article Title: Regulation of glypican 6-mediated Wnt activation maintains TDP-43 nuclear localization in neurons
    Article Snippet: .. All AAV plasmid backbones were based on AAV-GFP.Cre (Addgene, 49056). ..

    Injection:

    Article Title: Locus coeruleus norepinephrine neurons facilitate orbitofrontal cortex remapping and behavioral flexibility.
    Article Snippet: For rabies tracing experiments, AAV8-hSyn-FLEx loxP -TVA-mCherry and AAV8-hSyn-FLEx loxP -H2B-P2A-N2cG-WPRE were mixed at a 1:1 ratio prior to injecting 200 nL into the left OFC of Emx1 Cre (The Jackson Laboratory, 005628) and Gad2 Cre (The Jackson Laboratory, 010802) mice. .. Additional Dbh Cre KI (The Jackson Laboratory, 033951) mice (n = 2) were injected with Cre-dependent AAV encoding GFP (AAV-CAG-FLEx-eGFP, titer: 2.5*10 ̂13; Addgene. ..

    Knockdown:

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    shRNA:

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Sequencing:

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Clone Assay:

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Virus:

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Control:

    Article Title: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.
    Article Snippet: .. To knock down Sh3rf3, short-hairpin RNA (shRNA) sequence (CCTGGCTCTCTATGCATACAA) and ctrl shNC2 RNA (CCTAAGGTTAAGTCGCCCTCG) were cloned into GFP-tagged adeno-associated virus (AAV) -vector (Addgene) under the control of U6 promoter. .. Viral particles (titer: 5.23 × 1012 vg/mL) were produced by the Obio Technology (Shanghai) Corp., Ltd. To re-express Sh3rf3, the Sh3rf3 sequence (GeneID:237353) was cloned into the pAAV-hSyn-EGFP-P2A-tWPA vector.

    Recombinant:

    Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples.
    Article Snippet: .. After profiling our recombinant AAV samples, we next isolated genomes harboring a GFP expression cassette from an AAV preparation obtained from a commercial vendor (Addgene; pAAV-hSyn-eGFP) and prepared UMI-labeled amplicons for nanopore sequencing. ..

    Isolation:

    Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples.
    Article Snippet: .. After profiling our recombinant AAV samples, we next isolated genomes harboring a GFP expression cassette from an AAV preparation obtained from a commercial vendor (Addgene; pAAV-hSyn-eGFP) and prepared UMI-labeled amplicons for nanopore sequencing. ..

    Expressing:

    Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples.
    Article Snippet: .. After profiling our recombinant AAV samples, we next isolated genomes harboring a GFP expression cassette from an AAV preparation obtained from a commercial vendor (Addgene; pAAV-hSyn-eGFP) and prepared UMI-labeled amplicons for nanopore sequencing. ..

    Nanopore Sequencing:

    Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples.
    Article Snippet: .. After profiling our recombinant AAV samples, we next isolated genomes harboring a GFP expression cassette from an AAV preparation obtained from a commercial vendor (Addgene; pAAV-hSyn-eGFP) and prepared UMI-labeled amplicons for nanopore sequencing. ..



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