construct encoding memerald sec61b c1 (Addgene inc)
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construct encoding memerald sec61b c1
Construct Encoding Memerald Sec61b C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/construct+encoding+memerald+sec61b+c1/mEmerald-Sec61b-C1+(Plasmid+%2390992)/pmc09035751-46-13-24
Average 93 stars, based on 29 article reviews
Construct Encoding Memerald Sec61b C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/construct+encoding+memerald+sec61b+c1/mEmerald-Sec61b-C1+(Plasmid+%2390992)/pmc09035751-46-13-24
Average 93 stars, based on 29 article reviews
construct encoding memerald sec61b c1 - by Bioz Stars,
2026-10
93/100 stars
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Generated:Article Title: A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction. Article Snippet: Lentiviral plasmids were generated by inserting PCR fragments containing coding sequences of NFAST- and CFAST-containing halves flanking IRES element and mApple-6xHp into the lentiviral SJL12 backbone (provided by the St. Jude Vector Core) digested with AgeI and NotI restriction enzymes using InFusion cloning. .. APEX2-6xHp was generated by replacing mApple in mApple6xHp with APEX2 PCR fragments using the NheI and Article Title: Periodic ER-plasma membrane junctions support long-range Ca 2+ signal integration in dendrites. Article Snippet: .. pAAV hSyn INTRON mEmerald-Sec61b WPRE, pAAV hSyn INTRON HaloTag-Sec61b WPRE, pAAV hSyn INTRON mEmerald-ER WPRE, pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE, pAAV hSyn INTRON mScarlet-CAAX2 WPRE, pAAV hSyn INTRON HaloTag-STIM1 were generated subcloning the cORF of Polymerase Chain Reaction:Article Title: A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction. Article Snippet: Lentiviral plasmids were generated by inserting PCR fragments containing coding sequences of NFAST- and CFAST-containing halves flanking IRES element and mApple-6xHp into the lentiviral SJL12 backbone (provided by the St. Jude Vector Core) digested with AgeI and NotI restriction enzymes using InFusion cloning. .. APEX2-6xHp was generated by replacing mApple in mApple6xHp with APEX2 PCR fragments using the NheI and Plasmid Preparation:Article Title: Dual effect of α-synuclein disease variants on condensate formation Article Snippet: .. FSW_jGCaMP8f was a gift from Ronald Hart (Addgene plasmid # 197034; http://n2t.net/addgene:197034 ; RRID:Addgene_197034). pmScarlet_Giantin_C1 was a gift from Dorus Gadella (Addgene plasmid # 85048; http://n2t.net/addgene:85048 ; RRID:Addgene_85048) . Article Title: Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases Article Snippet: Ciliobrevin A (Tocris 4529) was used at a final concentration of 50 μM and added 1 hour prior to imaging. .. Rapalog A/C heterodimerizer (Takara, Cat # 635056) was added at 5 μM final concentration. mCherry-ER-3 (mCherry-KDEL; Addgene plasmid #55041) and mEmerald- TGNP-N-10 (Addgene plasmid #54279) were gifts from Michael Davidson. mCherrySec61-β was a gift from Gia Voeltz (Zurek et al., 2011; Addgene plasmid #49155). Article Title: Kinesin-1 mediates proper ER folding of the Ca V 1.2 channel and maintains mouse glucose homeostasis Article Snippet: For the phogrin - Dronpa-Green1 and phogrin-EGFP expression vectors, mouse phogrin cDNA was ligated with pDronpa-Green1 (MBL, Japan) or pEGFP-N1 (Clontech), and transferred to the ViraPower Adenoviral Expression System (Thermo Fisher). .. The Amplification:Article Title: Dual effect of α-synuclein disease variants on condensate formation Article Snippet: A third generation lentiviral FUGW vector (Addgene #197034) was used as a vector backbone to generate all lentiviral constructs used in this study. aSYN variants tagged to mScarlet-I and Synaptophysin-mEmerald were cloned downstream of the human Synapsin promoter using Gibson assembly (NEB, E2621S). .. Synaptophsyin fragment was amplified from cDNA derived from SH-SY5Y cells. mScarlet-I was derived from pmScarlet_Giantin_C1 (Addgene # 85048), mEmerald was derived from Derivative Assay:Article Title: Dual effect of α-synuclein disease variants on condensate formation Article Snippet: A third generation lentiviral FUGW vector (Addgene #197034) was used as a vector backbone to generate all lentiviral constructs used in this study. aSYN variants tagged to mScarlet-I and Synaptophysin-mEmerald were cloned downstream of the human Synapsin promoter using Gibson assembly (NEB, E2621S). .. Synaptophsyin fragment was amplified from cDNA derived from SH-SY5Y cells. mScarlet-I was derived from pmScarlet_Giantin_C1 (Addgene # 85048), mEmerald was derived from Concentration Assay:Article Title: Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases Article Snippet: Ciliobrevin A (Tocris 4529) was used at a final concentration of 50 μM and added 1 hour prior to imaging. .. Rapalog A/C heterodimerizer (Takara, Cat # 635056) was added at 5 μM final concentration. mCherry-ER-3 (mCherry-KDEL; Addgene plasmid #55041) and mEmerald- TGNP-N-10 (Addgene plasmid #54279) were gifts from Michael Davidson. mCherrySec61-β was a gift from Gia Voeltz (Zurek et al., 2011; Addgene plasmid #49155). Expressing:Article Title: Kinesin-1 mediates proper ER folding of the Ca V 1.2 channel and maintains mouse glucose homeostasis Article Snippet: For the phogrin - Dronpa-Green1 and phogrin-EGFP expression vectors, mouse phogrin cDNA was ligated with pDronpa-Green1 (MBL, Japan) or pEGFP-N1 (Clontech), and transferred to the ViraPower Adenoviral Expression System (Thermo Fisher). .. The Subcloning:Article Title: Periodic ER-plasma membrane junctions support long-range Ca 2+ signal integration in dendrites. Article Snippet: .. pAAV hSyn INTRON mEmerald-Sec61b WPRE, pAAV hSyn INTRON HaloTag-Sec61b WPRE, pAAV hSyn INTRON mEmerald-ER WPRE, pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE, pAAV hSyn INTRON mScarlet-CAAX2 WPRE, pAAV hSyn INTRON HaloTag-STIM1 were generated subcloning the cORF of Synthesized:Article Title: Periodic ER-plasma membrane junctions support long-range Ca 2+ signal integration in dendrites. Article Snippet: .. pAAV hSyn INTRON mEmerald-Sec61b WPRE, pAAV hSyn INTRON HaloTag-Sec61b WPRE, pAAV hSyn INTRON mEmerald-ER WPRE, pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE, pAAV hSyn INTRON mScarlet-CAAX2 WPRE, pAAV hSyn INTRON HaloTag-STIM1 were generated subcloning the cORF of |