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116 gradient concentration  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 116 gradient concentration
    116 Gradient Concentration, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/concentration+gradient/Matrine/pm41708019-70-7-71
    Average 94 stars, based on 1 article reviews
    116 gradient concentration - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: 1,2-Redox Transpositions of Tertiary Amides.
    Article Snippet: HRMS (ES+) exact mass calculated for [M+H]+ (C13H18NO) requires m/z 204.1380, found m/z 204.1383 Compound 3r Before use, commercial matrine (thermoscientific) was dissolved in Et2O (100 mg in 20 mL) and washed with sat. aq.

    Transfection:

    Article Title: Matrine functions as a tumor inhibitor to influence proliferation, metastasis and glycolysis in colorectal cancer via depending on METTL14-mediated m6A methylation of MEX3A.
    Article Snippet: Colorectal cancer (CRC) remains a serious health and economic burden exhibiting considerable incidence and death rate globally [1–3].. Most cases of CRC are usually diagnosed in aging adults, however, the recent evidence has suggested the morbidity is increasing among young adults [4].. Although the clinical treatment through a combination of the curative surgery and other therapies has become mature, some patients are still likely to relapse and have a poor prognosis [5, 6].

    Article Title: Protective role of matrine in sepsis-associated cardiac dysfunction through regulating the lncRNA PTENP1/miR-106b-5p axis.
    Article Snippet: .. In the [5–10] groups, in addition to matrine treatment, the cells were also transfected with the indicated vectors using Lipofectamine 3000 (Invitrogen, Thermo Fisher Scientific, Inc.) according to the manufacturer’s instruction. .. All the vectors for cell transfection were obtained from GenePharma (Shanghai, China).

    Plasmid Preparation:

    Article Title: Matrine functions as a tumor inhibitor to influence proliferation, metastasis and glycolysis in colorectal cancer via depending on METTL14-mediated m6A methylation of MEX3A.
    Article Snippet: Colorectal cancer (CRC) remains a serious health and economic burden exhibiting considerable incidence and death rate globally [1–3].. Most cases of CRC are usually diagnosed in aging adults, however, the recent evidence has suggested the morbidity is increasing among young adults [4].. Although the clinical treatment through a combination of the curative surgery and other therapies has become mature, some patients are still likely to relapse and have a poor prognosis [5, 6].

    Protease Inhibitor:

    Article Title: Src Acts as the Target of Matrine to Inhibit the Proliferation of Cancer Cells by Regulating Phosphorylation Signaling Pathways
    Article Snippet: Kinase activity assay The activity of Src kinase was measured according to the instructions of Src kinase activity detection kit (GENMED Scienti cs Inc., USA). .. Cells with matrine treatment were lysed supplemented with protease inhibitor cocktail (Thermo Scienti c, Rockford, IL, USA). .. The activity of Src kinase in cancer cells was quanti ed at a wavelength of 340 nm by SpectraMax i3x multifunctional microplate reader (Molecular Devices Corporation, CA, USA).

    Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways
    Article Snippet: The activity of Src kinase was measured according to the instructions of Src kinase activity detection kit (GENMED Scientifics Inc., USA). .. Cells with matrine treatment were lysed supplemented with protease inhibitor cocktail (Thermo Scientific, Rockford, IL, USA). .. The activity of Src kinase in cancer cells was quantified at a wavelength of 340 nm by a SpectraMax i3x multifunctional microplate reader (Molecular Devices Corporation, CA, USA).

    Activity Assay:

    Article Title: Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells
    Article Snippet: .. General lysosomal protease activity was measured by DQ green BSA (Thermo Scientific, USA), 1 × 10 6 /mL cells were incubated with 10 μg/mL DQ green BSA for 3 h at 37°C, 5% CO 2 , 0.8 mg/mL matrine was added and cultured for 24 h, and washed 3 times with PBS (Gibco, USA) for 5 min each, and fluorescence intensity was assessed using flow cytometry (BD FACS Canto, USA) [ ]. .. Data were analyzed using GraphPad Prism 7 software and SPSS 25.0.

    Incubation:

    Article Title: Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells
    Article Snippet: .. General lysosomal protease activity was measured by DQ green BSA (Thermo Scientific, USA), 1 × 10 6 /mL cells were incubated with 10 μg/mL DQ green BSA for 3 h at 37°C, 5% CO 2 , 0.8 mg/mL matrine was added and cultured for 24 h, and washed 3 times with PBS (Gibco, USA) for 5 min each, and fluorescence intensity was assessed using flow cytometry (BD FACS Canto, USA) [ ]. .. Data were analyzed using GraphPad Prism 7 software and SPSS 25.0.

    Article Title: Matrine inhibits invasion and migration of gallbladder cancer via regulating the PI3K/AKT signaling pathway.
    Article Snippet: Cell viability was evaluated according to Cell Counting Kit-8 (MedChemExpress, Cat. No. HY-K0301) provided by the vender. .. The cells (HGBEC, GBC-SD, NOZ, and SGC-996) were seeded in 96-well plates overnight and treated with different concentrations of Matrine (0–80 μM) for 12–72 h, or treated with LY294002 (0–80 μM)/740 Y-P(0–80 μM) for 24 h. Then, CCK8 solution with concentration of 10% was added to each well and incubated at 37 °C for 4 h. Finally, the absorbance of 450 nm was detected by Multiskan SkyHigh ThermoFisher, USA. ..

    Article Title: Matrine inhibits SGIV infection via regulation of the inflammatory and oxidative stress response.
    Article Snippet: 25 Matrine, a tetracyclic quinolizidine alkaloid derived from Sophora flavescens, 26 exhibited antiviral activity against several mammalian viruses.. Singapore grouper 27 iridovirus (SGIV) is a highly pathogenic agent that seriously threatens the sustainable 28 grouper industry, however, few efficient preventive measures were established up to 29 now.. Here, the antiviral effect of matrine against SGIV infection, and the potential 30 mechanism underlying its antiviral action was investigated in vitro.

    Cell Culture:

    Article Title: Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells
    Article Snippet: .. General lysosomal protease activity was measured by DQ green BSA (Thermo Scientific, USA), 1 × 10 6 /mL cells were incubated with 10 μg/mL DQ green BSA for 3 h at 37°C, 5% CO 2 , 0.8 mg/mL matrine was added and cultured for 24 h, and washed 3 times with PBS (Gibco, USA) for 5 min each, and fluorescence intensity was assessed using flow cytometry (BD FACS Canto, USA) [ ]. .. Data were analyzed using GraphPad Prism 7 software and SPSS 25.0.

    Fluorescence:

    Article Title: Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells
    Article Snippet: .. General lysosomal protease activity was measured by DQ green BSA (Thermo Scientific, USA), 1 × 10 6 /mL cells were incubated with 10 μg/mL DQ green BSA for 3 h at 37°C, 5% CO 2 , 0.8 mg/mL matrine was added and cultured for 24 h, and washed 3 times with PBS (Gibco, USA) for 5 min each, and fluorescence intensity was assessed using flow cytometry (BD FACS Canto, USA) [ ]. .. Data were analyzed using GraphPad Prism 7 software and SPSS 25.0.

    Flow Cytometry:

    Article Title: Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells
    Article Snippet: .. General lysosomal protease activity was measured by DQ green BSA (Thermo Scientific, USA), 1 × 10 6 /mL cells were incubated with 10 μg/mL DQ green BSA for 3 h at 37°C, 5% CO 2 , 0.8 mg/mL matrine was added and cultured for 24 h, and washed 3 times with PBS (Gibco, USA) for 5 min each, and fluorescence intensity was assessed using flow cytometry (BD FACS Canto, USA) [ ]. .. Data were analyzed using GraphPad Prism 7 software and SPSS 25.0.

    FACS:

    Article Title: Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells
    Article Snippet: .. General lysosomal protease activity was measured by DQ green BSA (Thermo Scientific, USA), 1 × 10 6 /mL cells were incubated with 10 μg/mL DQ green BSA for 3 h at 37°C, 5% CO 2 , 0.8 mg/mL matrine was added and cultured for 24 h, and washed 3 times with PBS (Gibco, USA) for 5 min each, and fluorescence intensity was assessed using flow cytometry (BD FACS Canto, USA) [ ]. .. Data were analyzed using GraphPad Prism 7 software and SPSS 25.0.

    Concentration Assay:

    Article Title: Matrine inhibits invasion and migration of gallbladder cancer via regulating the PI3K/AKT signaling pathway.
    Article Snippet: Cell viability was evaluated according to Cell Counting Kit-8 (MedChemExpress, Cat. No. HY-K0301) provided by the vender. .. The cells (HGBEC, GBC-SD, NOZ, and SGC-996) were seeded in 96-well plates overnight and treated with different concentrations of Matrine (0–80 μM) for 12–72 h, or treated with LY294002 (0–80 μM)/740 Y-P(0–80 μM) for 24 h. Then, CCK8 solution with concentration of 10% was added to each well and incubated at 37 °C for 4 h. Finally, the absorbance of 450 nm was detected by Multiskan SkyHigh ThermoFisher, USA. ..

    Article Title: Matrine inhibits SGIV infection via regulation of the inflammatory and oxidative stress response.
    Article Snippet: 25 Matrine, a tetracyclic quinolizidine alkaloid derived from Sophora flavescens, 26 exhibited antiviral activity against several mammalian viruses.. Singapore grouper 27 iridovirus (SGIV) is a highly pathogenic agent that seriously threatens the sustainable 28 grouper industry, however, few efficient preventive measures were established up to 29 now.. Here, the antiviral effect of matrine against SGIV infection, and the potential 30 mechanism underlying its antiviral action was investigated in vitro.

    CCK-8 Assay:

    Article Title: Matrine inhibits SGIV infection via regulation of the inflammatory and oxidative stress response.
    Article Snippet: 25 Matrine, a tetracyclic quinolizidine alkaloid derived from Sophora flavescens, 26 exhibited antiviral activity against several mammalian viruses.. Singapore grouper 27 iridovirus (SGIV) is a highly pathogenic agent that seriously threatens the sustainable 28 grouper industry, however, few efficient preventive measures were established up to 29 now.. Here, the antiviral effect of matrine against SGIV infection, and the potential 30 mechanism underlying its antiviral action was investigated in vitro.



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