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bruker microct  (Bruker Corporation)


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    Structured Review

    Bruker Corporation bruker microct
    a Schematic of experimental set-up. b Flow cytometry analysis of injury site 1 week after burn/tenotomy in mice treated with clodronate or PBS for monocytes (CD11b + Ly6G − ); neutrophils (CD11b + Ly6G + ); classical monocytes (CD11b + Ly6G − Ly6C hi ), alternatively activated monocytes (CD11b + Ly6G − Ly6C lo ) and macrophages (F4/80 + ). Circulating monocytes: n = 4/group, df = 8, t = −0.213, p = 0.000. Injury site: n = 4/group, df = 8. Neutrophils: t = −0.213,p = 0.837; Monocytes: t = 3.490, p = 0.008; Ly6C − : t = 3.193, p = 0.013; Ly6C low : t = 4.139, p = 0.003; Ly6C hi : t = −0.979, p = 0.356; F4/80 macrophages: t = 3.552, p = 0.007. c IVIS imaging of MPO activity 1 week after injury in mice treated with clodronate or control ( n = 4 mice per treatment). Total bioluminescent signal at the injury site using standardized region of interest (ROIs) was calculated and presented as total flux in photons per second per ROI. df = 3.182, t = 1.995, p = 0.135. d Left: representative images of ankle edema present in each treatment group. Right: quantification of ankle size. n (PBS) = 5, n (Clod) = 4, df = 7, t = 11.350, p = 0.000. e Representative Safranin O staining of tendon injury site 3 weeks after burn/tenotomy in clodronate and PBS treated mice. n = 3/group. f <t>MicroCT</t> analysis of tenotomy site 9 weeks after burn/tenotomy in clodronate and PBS control treated mice. Left: representative 3D reconstruction. Right: quantification of unthresholded total HO, floating HO (HO not associated with tibia or calcaneus) and proximal HO (HO proximal to the calcaneus). n (PBS) = 6, n (Clod) = 5. Total HO: t = 3.302, df = 5.312, p = 0.020; Floating HO: t = 1.867, df = 5.002, p = 0.121; Proximal HO: t = 1.313, df = 9, p = 0.222. All analyses assess for homoscedasticity and difference in means via Levene’s F-test and two-tailed Student’s t-test, respectively. * p < 0.05. Source data are provided as a Source Data file.
    Bruker Microct, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 10599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer-controlled+x-ray+diffractometer+xrd/Micro-CT/pmc07002453-298-9-9
    Average 99 stars, based on 10599 article reviews
    bruker microct - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing"

    Article Title: Regulation of heterotopic ossification by monocytes in a mouse model of aberrant wound healing

    Journal: Nature Communications

    doi: 10.1038/s41467-019-14172-4

    a Schematic of experimental set-up. b Flow cytometry analysis of injury site 1 week after burn/tenotomy in mice treated with clodronate or PBS for monocytes (CD11b + Ly6G − ); neutrophils (CD11b + Ly6G + ); classical monocytes (CD11b + Ly6G − Ly6C hi ), alternatively activated monocytes (CD11b + Ly6G − Ly6C lo ) and macrophages (F4/80 + ). Circulating monocytes: n = 4/group, df = 8, t = −0.213, p = 0.000. Injury site: n = 4/group, df = 8. Neutrophils: t = −0.213,p = 0.837; Monocytes: t = 3.490, p = 0.008; Ly6C − : t = 3.193, p = 0.013; Ly6C low : t = 4.139, p = 0.003; Ly6C hi : t = −0.979, p = 0.356; F4/80 macrophages: t = 3.552, p = 0.007. c IVIS imaging of MPO activity 1 week after injury in mice treated with clodronate or control ( n = 4 mice per treatment). Total bioluminescent signal at the injury site using standardized region of interest (ROIs) was calculated and presented as total flux in photons per second per ROI. df = 3.182, t = 1.995, p = 0.135. d Left: representative images of ankle edema present in each treatment group. Right: quantification of ankle size. n (PBS) = 5, n (Clod) = 4, df = 7, t = 11.350, p = 0.000. e Representative Safranin O staining of tendon injury site 3 weeks after burn/tenotomy in clodronate and PBS treated mice. n = 3/group. f MicroCT analysis of tenotomy site 9 weeks after burn/tenotomy in clodronate and PBS control treated mice. Left: representative 3D reconstruction. Right: quantification of unthresholded total HO, floating HO (HO not associated with tibia or calcaneus) and proximal HO (HO proximal to the calcaneus). n (PBS) = 6, n (Clod) = 5. Total HO: t = 3.302, df = 5.312, p = 0.020; Floating HO: t = 1.867, df = 5.002, p = 0.121; Proximal HO: t = 1.313, df = 9, p = 0.222. All analyses assess for homoscedasticity and difference in means via Levene’s F-test and two-tailed Student’s t-test, respectively. * p < 0.05. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Schematic of experimental set-up. b Flow cytometry analysis of injury site 1 week after burn/tenotomy in mice treated with clodronate or PBS for monocytes (CD11b + Ly6G − ); neutrophils (CD11b + Ly6G + ); classical monocytes (CD11b + Ly6G − Ly6C hi ), alternatively activated monocytes (CD11b + Ly6G − Ly6C lo ) and macrophages (F4/80 + ). Circulating monocytes: n = 4/group, df = 8, t = −0.213, p = 0.000. Injury site: n = 4/group, df = 8. Neutrophils: t = −0.213,p = 0.837; Monocytes: t = 3.490, p = 0.008; Ly6C − : t = 3.193, p = 0.013; Ly6C low : t = 4.139, p = 0.003; Ly6C hi : t = −0.979, p = 0.356; F4/80 macrophages: t = 3.552, p = 0.007. c IVIS imaging of MPO activity 1 week after injury in mice treated with clodronate or control ( n = 4 mice per treatment). Total bioluminescent signal at the injury site using standardized region of interest (ROIs) was calculated and presented as total flux in photons per second per ROI. df = 3.182, t = 1.995, p = 0.135. d Left: representative images of ankle edema present in each treatment group. Right: quantification of ankle size. n (PBS) = 5, n (Clod) = 4, df = 7, t = 11.350, p = 0.000. e Representative Safranin O staining of tendon injury site 3 weeks after burn/tenotomy in clodronate and PBS treated mice. n = 3/group. f MicroCT analysis of tenotomy site 9 weeks after burn/tenotomy in clodronate and PBS control treated mice. Left: representative 3D reconstruction. Right: quantification of unthresholded total HO, floating HO (HO not associated with tibia or calcaneus) and proximal HO (HO proximal to the calcaneus). n (PBS) = 6, n (Clod) = 5. Total HO: t = 3.302, df = 5.312, p = 0.020; Floating HO: t = 1.867, df = 5.002, p = 0.121; Proximal HO: t = 1.313, df = 9, p = 0.222. All analyses assess for homoscedasticity and difference in means via Levene’s F-test and two-tailed Student’s t-test, respectively. * p < 0.05. Source data are provided as a Source Data file.

    Techniques Used: Flow Cytometry, Imaging, Activity Assay, Staining, Two Tailed Test

    a Representative images of IVIS analysis for MPO of C57BL/6 J and LysMCre- Tgfb1 fl/fl mice bred on a C57BL/6 J background at 1 week after burn/tenotomy. Right: quantification of total bioluminescence/region of interest. n = 5/group. Injury site: t = 1.898, df = 8, p = 0.094; dorsum: t = 6.385, df = 4.007, p = 0.003. b Quantification of neutrophils and monocyte subpopulations based on Ly6C using flow cytometry of injury site 1 week after burn/tenotomy in C57BL/6 J and LysMCre- Tgfb1 fl/fl mice bred on a C57BL/6 J background. n = 4 mice/group. n = 3/group. Neutrophils: t = −1.125, fd = 4, p = 0.324; Ly6C - : t = 1.685, df = 4, p = 0.167; Ly6C lo : t = 0.315, df = 4, p = 0.768; Ly6C hi : t = −1.272, df = 4, p = 0.272. c MicroCT analysis of tenotomy site 9 weeks after burn/tenotomy in C57BL/6 J and LysMCre- Tgfb1 fl/fl mice. Left: Representative 3D reconstruction. Right: Quantification of total HO, floating HO (not associated with tibia or calcaneus) and proximal HO (all HO proximal to calcaneus). n = 5/group. Total HO: t = 2.290, df = 0, p = 0.051; Floating HO: t = 4.591, df = 4, p = 0.008; Proximal HO: t = 2.578, df = 8, p = 0.033. d Levels of TGFβ1 in pg/ug total protein from homogenates from the extremity injury and plasma from LysMCre- Tgfb1 fl/fl , LysMCre- Tgfb1 fl/wt , or wild type mice 3 days after burn/tenotomy n = 3 mice in each genotype. n = 3/group, df across groups = 2. F statistics: homogenate TGFB1: 0.588, plasma TGFB1: 1.008. All pairwise comparisons were analyzed for homoscedasticity and difference in means via Levene’s F-test and two-tailed Student’s t-test, respectively. Homoscedastic and heteroscedastic multi-group analyses were performed via ANOVA + post-hoc Dunnett’s test and Welch’s comparison of means + post-hoc Dunnett’s T3 test, respectively. * p < 0.05, ** p < 0.01. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Representative images of IVIS analysis for MPO of C57BL/6 J and LysMCre- Tgfb1 fl/fl mice bred on a C57BL/6 J background at 1 week after burn/tenotomy. Right: quantification of total bioluminescence/region of interest. n = 5/group. Injury site: t = 1.898, df = 8, p = 0.094; dorsum: t = 6.385, df = 4.007, p = 0.003. b Quantification of neutrophils and monocyte subpopulations based on Ly6C using flow cytometry of injury site 1 week after burn/tenotomy in C57BL/6 J and LysMCre- Tgfb1 fl/fl mice bred on a C57BL/6 J background. n = 4 mice/group. n = 3/group. Neutrophils: t = −1.125, fd = 4, p = 0.324; Ly6C - : t = 1.685, df = 4, p = 0.167; Ly6C lo : t = 0.315, df = 4, p = 0.768; Ly6C hi : t = −1.272, df = 4, p = 0.272. c MicroCT analysis of tenotomy site 9 weeks after burn/tenotomy in C57BL/6 J and LysMCre- Tgfb1 fl/fl mice. Left: Representative 3D reconstruction. Right: Quantification of total HO, floating HO (not associated with tibia or calcaneus) and proximal HO (all HO proximal to calcaneus). n = 5/group. Total HO: t = 2.290, df = 0, p = 0.051; Floating HO: t = 4.591, df = 4, p = 0.008; Proximal HO: t = 2.578, df = 8, p = 0.033. d Levels of TGFβ1 in pg/ug total protein from homogenates from the extremity injury and plasma from LysMCre- Tgfb1 fl/fl , LysMCre- Tgfb1 fl/wt , or wild type mice 3 days after burn/tenotomy n = 3 mice in each genotype. n = 3/group, df across groups = 2. F statistics: homogenate TGFB1: 0.588, plasma TGFB1: 1.008. All pairwise comparisons were analyzed for homoscedasticity and difference in means via Levene’s F-test and two-tailed Student’s t-test, respectively. Homoscedastic and heteroscedastic multi-group analyses were performed via ANOVA + post-hoc Dunnett’s test and Welch’s comparison of means + post-hoc Dunnett’s T3 test, respectively. * p < 0.05, ** p < 0.01. Source data are provided as a Source Data file.

    Techniques Used: Flow Cytometry, Two Tailed Test

    a Representative Safranin O stain of tendon injury site 3 weeks after burn/tenotomy in p7N3 (CD47 agonist) treated and PBS control mice. n = 3/group. b MicroCT analysis of tenotomy site 9 weeks after burn/tenotomy in PBS and p7N3 (CD47 agonist) treated mice. Left: Representative 3D reconstruction. Right: Quantification of total HO, floating HO and proximal HO. n = 7/group. Total HO: t = 3.415, df = 7.840, p = 0.009; Floating HO: t = 2.201, df = 12, p = 0.048; Proximal HO: t = 2.686, df = 8.549, p = 0.026. c Levels of TGF-β1, TGFβ2, and TGFβ3 in pg/ug total protein and represented as median with interquartile range from Top: homogenates from the extremity injury (TGF-β1: t = −0.635, df = 4, p = 0.560; TGF-β2: t = −0.643, df = 4, p = 0.555; TGF-β3: t = −1.272, df = 2.186, p = 0.322) and Bottom: plasma from PBS and p7N3 (CD47) peptide treated mice 3 days after burn/tenotomy (TGF-β1: t = 1.544, df = 2.037, p = 0.260; TGF-β2: t = 2.747, df = 4, p = 0.052; TGF-β3: t = −1.492, df = 4, p = 0.210). n = 3 mice per treatment group. d qPCR analysis of M1 ( iNos ) and M2 ( Arg1 and Mrc1 ) macrophage markers and Tgfb1 in macrophages isolated from the extremity injury site of naive (day 0), burn/tenotomy day 3, burn/tenotomy day 3 treated with PBS, and burn/tenotomy day 3 treated with p7N3 (CD47) peptide. Day 0 vs. Day 3— iNOS : t = 2.020, df = 2, p = 0.181; Arg1 : t = −6.084, df = 3, p = 0.009; Mrc1 : t = 0.703, df = 4, p = 0.521; Tgfb1 : t = 0.253, df = 4, p = 0.812. PBS vs. CD47 – iNOS : t = −0.834, df = 2.043, p = 0.491; Arg1 : t = 0.895, df = 4, p = 0.421; Mrc1 : t = 1.176, df = 4, p = 0.305; Tgfb1 : t = 1.186, df = 4, p = 0.301. e Representative images of phagocytosis assay using macrophages isolated from the extremity injury at day 3 after burn/tenotomy in mice treated with PBS or p7N3 (CD47). PBS n = 3, CD47 n = 4 approximately 25 cells/mouse. f Measurement of cellular circularity Circularity: t = 6.119, df = 55.537, p = 0.000 and quantification of mean fluorescent intensity phagocytosed by each macrophage. MFI: t = −0.357, df = 111, p = 0.722. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Representative Safranin O stain of tendon injury site 3 weeks after burn/tenotomy in p7N3 (CD47 agonist) treated and PBS control mice. n = 3/group. b MicroCT analysis of tenotomy site 9 weeks after burn/tenotomy in PBS and p7N3 (CD47 agonist) treated mice. Left: Representative 3D reconstruction. Right: Quantification of total HO, floating HO and proximal HO. n = 7/group. Total HO: t = 3.415, df = 7.840, p = 0.009; Floating HO: t = 2.201, df = 12, p = 0.048; Proximal HO: t = 2.686, df = 8.549, p = 0.026. c Levels of TGF-β1, TGFβ2, and TGFβ3 in pg/ug total protein and represented as median with interquartile range from Top: homogenates from the extremity injury (TGF-β1: t = −0.635, df = 4, p = 0.560; TGF-β2: t = −0.643, df = 4, p = 0.555; TGF-β3: t = −1.272, df = 2.186, p = 0.322) and Bottom: plasma from PBS and p7N3 (CD47) peptide treated mice 3 days after burn/tenotomy (TGF-β1: t = 1.544, df = 2.037, p = 0.260; TGF-β2: t = 2.747, df = 4, p = 0.052; TGF-β3: t = −1.492, df = 4, p = 0.210). n = 3 mice per treatment group. d qPCR analysis of M1 ( iNos ) and M2 ( Arg1 and Mrc1 ) macrophage markers and Tgfb1 in macrophages isolated from the extremity injury site of naive (day 0), burn/tenotomy day 3, burn/tenotomy day 3 treated with PBS, and burn/tenotomy day 3 treated with p7N3 (CD47) peptide. Day 0 vs. Day 3— iNOS : t = 2.020, df = 2, p = 0.181; Arg1 : t = −6.084, df = 3, p = 0.009; Mrc1 : t = 0.703, df = 4, p = 0.521; Tgfb1 : t = 0.253, df = 4, p = 0.812. PBS vs. CD47 – iNOS : t = −0.834, df = 2.043, p = 0.491; Arg1 : t = 0.895, df = 4, p = 0.421; Mrc1 : t = 1.176, df = 4, p = 0.305; Tgfb1 : t = 1.186, df = 4, p = 0.301. e Representative images of phagocytosis assay using macrophages isolated from the extremity injury at day 3 after burn/tenotomy in mice treated with PBS or p7N3 (CD47). PBS n = 3, CD47 n = 4 approximately 25 cells/mouse. f Measurement of cellular circularity Circularity: t = 6.119, df = 55.537, p = 0.000 and quantification of mean fluorescent intensity phagocytosed by each macrophage. MFI: t = −0.357, df = 111, p = 0.722. Source data are provided as a Source Data file.

    Techniques Used: Staining, Isolation, Phagocytosis Assay

    Related Articles

    Control:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Ex Vivo:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Micro-CT:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Article Title: Moxibustion Regulates the Imbalance of Cartilage Degradation and Repair in Knee Osteoarthritis Rabbits via the SDF-1/CXCR4 Axis: Is Stimulating Heat-Sensitized Acupoints More Efficacious?
    Article Snippet: Band intensity was analyzed using ImageJ software, with GAPDH serving as the internal reference protein. .. The right knee joints of the New Zealand rabbits were fixed in 4% paraformaldehyde (Servicebio, Guangzhou, China) for 24 hours and subsequently scanned using a Micro-CT system (Bruker, Germany). ..

    Article Title: Anti-migratory chitosan/BaSO4 markers with hydration in situ for long-term multimodal breast tissue imaging
    Article Snippet: Finally, compression tests (crosshead speed 1 mm/min) were conducted on the hydrated samples using a universal testing machine (Instron 34SC-1, USA) to assess their compressive strength and toughness under simulated physiological conditions (PBS, pH 7.4, 37 ◦C). .. The xerogel samples, both in their initial dry state (5 mm in length and 1.5 mm in diameter) and corresponding hydrated state, were imaged via a high-resolution micro-CT scanner (Bruker SkyScan 1272, USA). ..

    Article Title: OXR1 is a dual redox-mitochondrial checkpoint governing osteoclast differentiation and bone loss in an osteoporosis mouse model.
    Article Snippet: .. Reconstruction of the acquired images was performed with CTvox software (Bruker microCT, Kontich, Belgium), utilizing a ring artifact correction of 7, a smoothing factor of 2, and a beam hardening correction of 40%. ..

    Article Title: Divergent pathways of straw versus biochar integration with manure to enhance SOC sequestration in coastal saline soils
    Article Snippet: .. Three representative large macroaggregates from each sample were scanned using a micro-CT scanner (Bruker Skyscan 1272, Germany) at 100 keV and 100 μA. ..

    Article Title: Establishment of a novel asymmetric force degenerative lumbar scoliosis aging bipedal rat model
    Article Snippet: .. After scanning, the micro-CT images of the spinal specimens were imported into the NRecon reconstruction software (version 1.7; Bruker Corporation) to obtain cross-sectional images of the L1-L6 vertebrae of the rat spinal specimens. ..

    Imaging:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Software:

    Article Title: OXR1 is a dual redox-mitochondrial checkpoint governing osteoclast differentiation and bone loss in an osteoporosis mouse model.
    Article Snippet: .. Reconstruction of the acquired images was performed with CTvox software (Bruker microCT, Kontich, Belgium), utilizing a ring artifact correction of 7, a smoothing factor of 2, and a beam hardening correction of 40%. ..

    Article Title: Establishment of a novel asymmetric force degenerative lumbar scoliosis aging bipedal rat model
    Article Snippet: .. After scanning, the micro-CT images of the spinal specimens were imported into the NRecon reconstruction software (version 1.7; Bruker Corporation) to obtain cross-sectional images of the L1-L6 vertebrae of the rat spinal specimens. ..

    other:

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: The fixed femoral specimens were examined and imaged using Micro-CT (Skyscan 1276, Bruker, Kontich, Belgium) to evaluate the newly formed bone within the defect area.



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