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computer assisted analysis  (Olympus)


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    Structured Review

    Olympus computer assisted analysis
    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Computer Assisted Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 10297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer-assisted+image+analysis/cellSens+Imaging+Software/pmc12939340-97-8-16
    Average 99 stars, based on 10297 article reviews
    computer assisted analysis - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model"

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    Journal: Cells

    doi: 10.3390/cells15040360

    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Figure Legend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Techniques Used: Immunodetection, TUNEL Assay

    Related Articles

    Imaging:

    Article Title: PINK1 and PARKIN Deficiency Increases Neuronal Vulnerability to Autoimmune Neuroinflammation Through Mitochondrial Fragmentation in Aged Mice
    Article Snippet: Sections were then mounted on grids and examined using a JEM‐1400 Flash electron microscope (JEOL, Japan). .. Mitochondria within dopaminergic neurons were manually quantified and measured for area using cellSens imaging analysis software (Olympus, Japan). .. Mitochondria were isolated from midbrain tissue using the Mitochondria Isolation Kit for Tissue (Cat. No. 89801, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions.

    Article Title: Tangeretin protects against nodularin-R-induced hepatotoxicity by modulating oxidative stress, inflammation, and apoptosis.
    Article Snippet: Nodularin (NOD) is a cyanobacterial toxin that induces severe biochemical and structural alterations in the liver.. This study aimed to investigate the protective effects of tangeretin (TAN) against NOD-R-induced hepatotoxicity by evaluating biochemical, oxidative stress, histopathological, and immunohistochemical alterations in a murine model. Fifty-six male mice were randomly allocated to four experimental groups (n = 14/group): Control, TAN, NOD-R, and TAN + NOD-R.. The control, TAN, and NOD-R groups received intraperitoneal (i.p.) injections of 0.9% saline containing 0.1% dimethyl sulfoxide (DMSO), TAN (100 mg/kg b.w., 0.9% saline 0.1% DMSO), and NOD-R (15 μg/kg b.w., 0.9% saline), respectively, for 7 consecutive days.

    Analysis:

    Article Title: PINK1 and PARKIN Deficiency Increases Neuronal Vulnerability to Autoimmune Neuroinflammation Through Mitochondrial Fragmentation in Aged Mice
    Article Snippet: Sections were then mounted on grids and examined using a JEM‐1400 Flash electron microscope (JEOL, Japan). .. Mitochondria within dopaminergic neurons were manually quantified and measured for area using cellSens imaging analysis software (Olympus, Japan). .. Mitochondria were isolated from midbrain tissue using the Mitochondria Isolation Kit for Tissue (Cat. No. 89801, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions.

    Software:

    Article Title: PINK1 and PARKIN Deficiency Increases Neuronal Vulnerability to Autoimmune Neuroinflammation Through Mitochondrial Fragmentation in Aged Mice
    Article Snippet: Sections were then mounted on grids and examined using a JEM‐1400 Flash electron microscope (JEOL, Japan). .. Mitochondria within dopaminergic neurons were manually quantified and measured for area using cellSens imaging analysis software (Olympus, Japan). .. Mitochondria were isolated from midbrain tissue using the Mitochondria Isolation Kit for Tissue (Cat. No. 89801, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions.

    Article Title: Prostaglandin-Endoperoxide Synthase 2 (PTGS2) Expression Is Increased in Chronic Asymptomatic Orchitis in the Dog
    Article Snippet: .. For the interstitial compartment, 20 randomly chosen images (10 for each side in CAO) were taken at 400× magnification (Olympus BX41TF) using the Olympus cellSens Dimension software (version 2.1). ..

    Article Title: A microbiome-bile acid axis mediates protection from chronic liver disease in females.
    Article Snippet: High-resolution live-cell fluorescent images were captured using an Olympus SpinSR10 spinning disk confocal super-resolution microscope equipped with a 100x oil-immersion objective. .. Image acquisition was performed using the cellSens software (Olympus, Tokyo, Japan). ..

    Article Title: Tangeretin protects against nodularin-R-induced hepatotoxicity by modulating oxidative stress, inflammation, and apoptosis.
    Article Snippet: Nodularin (NOD) is a cyanobacterial toxin that induces severe biochemical and structural alterations in the liver.. This study aimed to investigate the protective effects of tangeretin (TAN) against NOD-R-induced hepatotoxicity by evaluating biochemical, oxidative stress, histopathological, and immunohistochemical alterations in a murine model. Fifty-six male mice were randomly allocated to four experimental groups (n = 14/group): Control, TAN, NOD-R, and TAN + NOD-R.. The control, TAN, and NOD-R groups received intraperitoneal (i.p.) injections of 0.9% saline containing 0.1% dimethyl sulfoxide (DMSO), TAN (100 mg/kg b.w., 0.9% saline 0.1% DMSO), and NOD-R (15 μg/kg b.w., 0.9% saline), respectively, for 7 consecutive days.

    Article Title: PRDX4 attenuates mitochondrial dysfunction by targeting CALR/SERCA2 interaction to suppress mitochondrial apoptosis in laryngeal squamous cell carcinoma.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: .. Immunofluorescence images were captured at ×40 magnification using an Olympus FV2000 Confocal Microscope with cellSens software (Olympus Corporation of the Americas, Center Valley, PA, USA). ..

    Fluorescence:

    Article Title: Cell wall metabolism and cellular structure synergistically regulate texture in jujube fruit: implications for quality classification and processing adaptability.
    Article Snippet: The following day, after embedding and polymerization, the samples were sectioned, stained with uranyl acetate and lead citrate, and observed using a Hitachi TEM system. .. Images of jujube pulp cell sections were acquired using a BX53 fluorescence microscopy (Olympus Corporation, Tokyo, Japan) and analyzed and measured using the OLYMPUS-cellsens 2.1 system (Build 17342). ..

    Microscopy:

    Article Title: Cell wall metabolism and cellular structure synergistically regulate texture in jujube fruit: implications for quality classification and processing adaptability.
    Article Snippet: The following day, after embedding and polymerization, the samples were sectioned, stained with uranyl acetate and lead citrate, and observed using a Hitachi TEM system. .. Images of jujube pulp cell sections were acquired using a BX53 fluorescence microscopy (Olympus Corporation, Tokyo, Japan) and analyzed and measured using the OLYMPUS-cellsens 2.1 system (Build 17342). ..

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: .. Immunofluorescence images were captured at ×40 magnification using an Olympus FV2000 Confocal Microscope with cellSens software (Olympus Corporation of the Americas, Center Valley, PA, USA). ..

    Immunofluorescence:

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: .. Immunofluorescence images were captured at ×40 magnification using an Olympus FV2000 Confocal Microscope with cellSens software (Olympus Corporation of the Americas, Center Valley, PA, USA). ..

    Confocal:

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: .. Immunofluorescence images were captured at ×40 magnification using an Olympus FV2000 Confocal Microscope with cellSens software (Olympus Corporation of the Americas, Center Valley, PA, USA). ..



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    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay