computer-based image-analysis system  (Medical Imaging Applications LLC)

Journal: Frontiers in Plant Science

Article Title: PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro

doi: 10.3389/fpls.2018.00219

Figure Lengend Snippet: Use of PlantSize to measure rosette sizes of Arabidopsis plants. (A) Dialog box of PlantSize with imported images of young wild type and mutant Arabidopsis plants. (B) Linear correlation of fresh weights and rosette sizes of individual Arabidopsis plants, grown on standard culture medium. Note high level of correlation between rosette sizes (shown in pixel numbers) and fresh weights (FW) of individual plants. (C) Change of average fresh weights of wild type Arabidopsis plants in 14 days growth period. (D) Change of average rosette sizes of wild type Arabidopsis plants in 14 days growth period, as determined by PlantSize. (E) Linear correlation of average fresh weights and average rosette sizes displayed on C,D .

Article Snippet: Images are analyzed with the MatLab-based computer application PlantSize, which simultaneously calculates several parameters including rosette size, convex area, convex ratio, chlorophyll and anthocyanin contents of all plants identified on the image.

Techniques: Mutagenesis

Journal: Frontiers in Plant Science

Article Title: PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro

doi: 10.3389/fpls.2018.00219

Figure Lengend Snippet: Repression of Arabidopsis growth by increasing concentrations of salt. Five days-old seedlings were transferred to media supplemented by different concentrations of NaCl. Growth was monitored either by rosette imaging or weight measurements. (A) Rosette sizes of Arabidopsis plants determined by imaging and PlantSize analysis. (B) Average growth rates of plants calculated by the “Logest” function of Excel. (C,D) Average fresh weights (FW) and dry weights (DW) of plants grown on saline media. Bars on diagrams indicate standard deviation, ∗ shows significant differences to control tested by one-way ANOVA ( p < 0.05).

Article Snippet: Images are analyzed with the MatLab-based computer application PlantSize, which simultaneously calculates several parameters including rosette size, convex area, convex ratio, chlorophyll and anthocyanin contents of all plants identified on the image.

Techniques: Imaging, Saline, Standard Deviation, Control

Journal: Frontiers in Plant Science

Article Title: PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro

doi: 10.3389/fpls.2018.00219

Figure Lengend Snippet: Correlation of chlorophyll contents with Hue values. Fourteen days-old Arabidopsis plants treated by different stresses: 0,1 mM CdCl 2 , 3 mM H 2 O 2 , 150 mM NaCl, known to affect chlorophyll content. Plants were photographed after 3 days, and images were analyzed by PlantSize. (A) Images of treated plants. (B) Linear correlation of Hue values and chlorophyll contents of individual Arabidopsis plants (pixel numbers were used according to Figure ). (C,D) Average chlorophyll contents and HUE values of treated plants. (E) Correlation of average chlorophyll contents and average HUE values. Bars on diagrams show standard deviation, ∗ Indicates significant differences to control tested by one-way ANOVA ( p < 0.05).

Article Snippet: Images are analyzed with the MatLab-based computer application PlantSize, which simultaneously calculates several parameters including rosette size, convex area, convex ratio, chlorophyll and anthocyanin contents of all plants identified on the image.

Techniques: Standard Deviation, Control

Journal: Frontiers in Plant Science

Article Title: PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro

doi: 10.3389/fpls.2018.00219

Figure Lengend Snippet: Estimation of anthocyanin accumulation in Arabidopsis plants. Fourteen days-old Arabidopsis plants were treated by 0,1 mM CdCl 2 , 3 mM H 2 O 2 , 200 mM, and 500 mM sucrose, known to stimulate anthocyanin accumulation. Plants were photographed after 3 days and images were analyzed by PlantSize. (A) Images of plants with different anthocyanin content. (B) Reverse correlation of Anthocyanin contents and HUE values of individual Arabidopsis plants (pixel numbers were used according to Figure ). (C,D) Average anthocyanin contents and average HUE values of treated plants. (E) Reverse correlation of average anthocyanin contents and HUE values. Bars on diagrams indicate standard deviation, ∗ shows significant differences to control tested by one-way ANOVA ( p < 0.05).

Article Snippet: Images are analyzed with the MatLab-based computer application PlantSize, which simultaneously calculates several parameters including rosette size, convex area, convex ratio, chlorophyll and anthocyanin contents of all plants identified on the image.

Techniques: Standard Deviation, Control

Journal: Frontiers in Plant Science

Article Title: PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro

doi: 10.3389/fpls.2018.00219

Figure Lengend Snippet: Heat shock factor A4A (HSFA4A) modulates stress tolerance. (A) 5-days-old HSFA4A overexpressing seedlings (lines HSFox1, HSFox2) and wild type plants were transferred to culture medium supplemented by 100 mM NaCl. Growth was monitored by periodic imaging and evaluated by PlantSize. (A) Relative rosette sizes of plants grown on standard culture medium (1 corresponds to pixel No. on day 0). (B) Plant growth on saline medium. (C,D) 14-days-old plants were transferred to medium containing 150 mM NaCl and photographed at daily intervals. Changes in chlorophyll (C) and anthocyanin (D) contents were calculated from shifts in HUE values of the color images. Relative values are shown, where 1 corresponds to values of non-treated plants (day 0). Error bars indicate standard deviation, ∗ shows significant differences to control tested by one-way ANOVA ( p < 0.05).

Article Snippet: Images are analyzed with the MatLab-based computer application PlantSize, which simultaneously calculates several parameters including rosette size, convex area, convex ratio, chlorophyll and anthocyanin contents of all plants identified on the image.

Techniques: Imaging, Saline, Standard Deviation, Control

Journal: Frontiers in Plant Science

Article Title: PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro

doi: 10.3389/fpls.2018.00219

Figure Lengend Snippet: Comparison of PlantSize with other imaging tools, developed for quantification of different plant parameters.

Article Snippet: Images are analyzed with the MatLab-based computer application PlantSize, which simultaneously calculates several parameters including rosette size, convex area, convex ratio, chlorophyll and anthocyanin contents of all plants identified on the image.

Techniques: Comparison, Imaging, Software, In Vitro