complementary dna cdna libraries (Roche)

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Roche complementary dna cdna libraries
Complementary Dna Cdna Libraries, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complementary+dna+libraries/HyperPrep/pmc12506486-27-2-31
Average 99 stars, based on 5 article reviews

complementary dna cdna libraries - by Bioz Stars, 2026-09

99/100 stars

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Isolation:

Article Title: Supersensitive and robust disease monitoring in oropharyngeal cancer patients by circulating tumor HPV-DNA sequencing (ctHPV-DNAseq)
Article Snippet: .. In brief, DNA was isolated by Qiagen columns and prepared for sequencing using the KAPA HyperPrep kit (Roche Diagnostics Nederland BV, Almere, The Netherlands). ..

Sequencing:

Article Title: Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency
Article Snippet: The reaction was incubated for a further 20 min at 50 °C, and heat inactivated at 85 °C for 5 min. For producer-cell RNA only, the RT products were further processed by hydrolyzing the RNA in 0.2 M NaOH (final concentration) and heating at 90 °C for 10 min, cleaning up with the MinElute Reaction Cleanup Kit (Qiagen; 28104) according to the manufacturer’s protocols, and left-sided size selection using SPRIselect (Beckman Coulter; B23317 ) at a 1.5× bead ratio to remove smaller products and residual primers. .. Producer-cell cDNAs were subsequently amplified for sequencing with the KAPA HiFi HotStart ReadyMix (Roche Diagnostics; 09420398001) using 2.5 μL of cDNA input per 25 μL reaction and the following conditions: 95 °C for 3 min; 20 cycles of 98 °C for 20 s, 61 °C for 20 s, and 72 °C for 30 s; 72 °C for 2 min. eVLP cDNAs were amplified for sequencing with the KAPA HiFi HotStart ReadyMix using 2.5 μL of cDNA input per 25 μL reaction and the following conditions: 95 °C for 3 min; 14 cycles of 98 °C for 20 s, 61 °C for 20 s, and 72 °C for 30 s; 72 °C for 2 min. Illumina barcodes were installed using KAPA HiFi HotStart ReadyMix using 2 μL of PCR1 input per 25 μL reaction and the following conditions: 95 °C for 3 min; 9 cycles of 98 °C for 20 s, 61 °C for 20 s, and 72 °C for 30 s; 72 °C for 2 min. .. Following PCR2, samples were gel purified in a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen; 28704).

Article Title: Concomitant Clonal CBFB :: MYH11 and PDGFRB Fusions in a Case of De Novo Acute Myeloid Leukemia.
Article Snippet: RNA was extracted from the bone marrow specimen using the Maxwell® RSC simplyRNA Blood kit (Promega, Madison, WI, USA), converted to double-stranded cDNA, and purified. .. Sequencing library was prepared using the KAPA HyperPrep kit (Roche, Basel, Switzerland). .. Target enrichment was performed with xGen capture probes (Integrated DNA Technologies, Coralville, IA, USA) targeting the coding exons of the 50-gene panel.

Article Title: Coptidis Rhizoma extract mitigates sleep deprivation-induced cognitive impairment and neurodegeneration: Insights into hormonal, glymphatic, and molecular mechanisms
Article Snippet: RNA integrity and degradation were further monitored using a Qsep 100 DNA/RNA Analyzer (BiOptic Inc., Taiwan). .. For sequencing library preparation, total RNA was processed with the KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland). .. High-throughput sequencing was conducted using the Illumina NovaSeq 6000 platform, generating raw sequence data.

Construct:

Article Title: The complete mitochondrial genome of Bionychiurus tamilensis (Collembola: Onychiuridae)
Article Snippet: .. A genomic DNA library was constructed using the KAPA Hyper Prep Kit (Kapa Biosystems, Woburn, MA) and sequenced with paired-end reads on the Illumina Nextseq1000 platform (Illumina Inc., San Diego, CA). .. The mitogenome was assembled using a two-step process in Geneious Prime v.2025.0.3 (Biomatters Ltd, Auckland, New Zealand).

Amplification:

RNA Sequencing:

Article Title: The dynamics of centromere assembly and disassembly during quiescence
Article Snippet: .. For RNA sequencing: rRNA depletion and library preparation were done with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (KK8560; Roche) according to the manufacturer’s instructions. .. Sequencing was performed on an Illumina NovaSeq SP.

other:

Article Title: Light-inducible CRISPR/Cas9 system for genome editing
Article Snippet: Next Generation Amplicon Sequencing for Measuring Insertions or Deletions Genomic DNA samples were amplified with PCR using Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs M0494).

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