Review




Structured Review

Becton Dickinson knockout rnai system
Knockout Rnai System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complement+functional+screen+elisa+kit/knockout+rnai+system/us07638490-416-12-15
Average 90 stars, based on 1 article reviews
knockout rnai system - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Knockdown:

Article Title: Glypican-3-specific antibody and uses thereof
Article Snippet: Membranes were blotted with primary Abs followed by incubation with infrared dye IR680-labeled secondary antibodies and quantified using LI-COR Odyssey software. .. Glypican-3 Knockdown hGPC3-specific short hairpin RNAs (shRNAs) were prepared in the pSIREN-retroQ-zsGreen retroviral vector using BD knockout RNAi systems according to the manufacturer's instruction (Clontech). .. Three pairs of 21 nt oligonucleotides, named sh56, sh57, and sh58 as well as a LacZ (negative control), were predicted according to Ambion SILENCER® Select software, annealed and subcloned into pSIREN-retroQ-zsGreen at the BamHI and EcoRI sites.

Retroviral:

Article Title: Glypican-3-specific antibody and uses thereof
Article Snippet: Membranes were blotted with primary Abs followed by incubation with infrared dye IR680-labeled secondary antibodies and quantified using LI-COR Odyssey software. .. Glypican-3 Knockdown hGPC3-specific short hairpin RNAs (shRNAs) were prepared in the pSIREN-retroQ-zsGreen retroviral vector using BD knockout RNAi systems according to the manufacturer's instruction (Clontech). .. Three pairs of 21 nt oligonucleotides, named sh56, sh57, and sh58 as well as a LacZ (negative control), were predicted according to Ambion SILENCER® Select software, annealed and subcloned into pSIREN-retroQ-zsGreen at the BamHI and EcoRI sites.

Knock-Out:

Article Title: Glypican-3-specific antibody and uses thereof
Article Snippet: Membranes were blotted with primary Abs followed by incubation with infrared dye IR680-labeled secondary antibodies and quantified using LI-COR Odyssey software. .. Glypican-3 Knockdown hGPC3-specific short hairpin RNAs (shRNAs) were prepared in the pSIREN-retroQ-zsGreen retroviral vector using BD knockout RNAi systems according to the manufacturer's instruction (Clontech). .. Three pairs of 21 nt oligonucleotides, named sh56, sh57, and sh58 as well as a LacZ (negative control), were predicted according to Ambion SILENCER® Select software, annealed and subcloned into pSIREN-retroQ-zsGreen at the BamHI and EcoRI sites.

Article Title: Forkhead Box O3a requires BAF57, a subunit of chromatin remodeler SWI/SNF complex for induction of p53 up-regulated modulator of apoptosis (Puma) in a model of Parkinson's disease.
Article Snippet: Parkinson’s disease (PD) results from the selective loss of dopaminergic neurons of substantia nigra pars compacta region of the midbrain.. It has been reported that the transcription factor forkhead Box O3a (FoxO3a) is activated and induces pro-apoptotic protein such as BIM (Bcl-2 interacting mediator of cell death) and PUMA (p53 upregulated modulator of apoptosis) in variety of neuron death paradigms.. Activity of FoxO3a is governed by its post-translational modifications which control its sub-cellular localization.

Article Title: Bcl-2–associated athanogene 3 protects the heart from ischemia/reperfusion injury
Article Snippet: .. Ad-GFP, Ad-BAG3, or Ad-siBAG3 was constructed using the BD Adeno-X Expression System, 2PT3674-1, and BD Knockout RNAi Systems, PT3739 (BD Biosciences-Clontech) as previously described ( 9 ). ..

Article Title: Smokeless tobacco induces toxicity and apoptosis in neuronal cells: a mechanistic evaluation.
Article Snippet: Smokeless tobacco (SLT) or chewing tobacco has been a highly addictive practice in India across ages, posing major threat to the systemic health and possibly neurodegeneration.. Earlier studies showed components of SLT could be harmful to neuronal health.. However, mechanism of SLT in neurodegeneration remained unexplored.

Article Title: The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.
Article Snippet: Accepted Manuscript The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas PII: S1044-7431(17)30370-6 DOI: doi:10.1016/j.mcn.2018.02.011 Reference: YMCNE 3287 To appear in: Molecular and Cellular Neuroscience Received date: 20 November 2017 Revised date: 23 February 2018 Accepted date: 25 February 2018 Please cite this article as: Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas , The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

Article Title: Lrp4/corin dopamine-producing neuron precursor cell marker
Article Snippet: The nucleotide sequence of an siRNA can be designed using a computer program such as that disclosed at the Ambion website (http://www.ambion.com/techlib/misc/siRNA_finder.html). .. Kits for screening for functional siRNAs are also commercially available and can be used (for example, BD Knockout RNAi System (BD Biosciences Clontech). .. 228: 225; M. Koizumi and E. Ohtsuka (1990) Protein, Nucleic Acid and Enzyme 35: 2191; Koizumi et al. (1989) Nucleic Acids Res.

Article Title: BAG3 regulates contractility and Ca 2+ homeostasis in adult mouse ventricular myocytes
Article Snippet: .. BAG3shRNA-Ad construct was made using the BD Adeno-X Expression Systems 2PT3674-1 (Pr36024) and BD knockout RNAi Systems PT3739 (PR42756)(BD Biosciences-Clontech, Palo Alto, CA) as previously described [ 15 ]. .. A dsDNA oligonucleotide against a specific BAG3 mRNA (5′-AAG GUU CAG ACC AUC UUG GAA-3′) was inserted in a RNAi-ready pSIREN-DNR vector designed to express a small hairpin RNA (shRNA) driven by the human Pol III-dependent U6 promoter.

Article Title: Cardiac Dysfunction in HIV-1 Transgenic Mouse: Role of Stress and BAG3
Article Snippet: .. BAG3shRNA‐Ad construct was made using the BD Adeno‐X Expression Systems 2PT3674‐1 (Pr36024) and BD knockout RNAi Systems PT3739 (PR42756)(BD Biosciences‐Clontech, Palo Alto, CA, USA) as previously described. ..

shRNA:

Article Title: Forkhead Box O3a requires BAF57, a subunit of chromatin remodeler SWI/SNF complex for induction of p53 up-regulated modulator of apoptosis (Puma) in a model of Parkinson's disease.
Article Snippet: Parkinson’s disease (PD) results from the selective loss of dopaminergic neurons of substantia nigra pars compacta region of the midbrain.. It has been reported that the transcription factor forkhead Box O3a (FoxO3a) is activated and induces pro-apoptotic protein such as BIM (Bcl-2 interacting mediator of cell death) and PUMA (p53 upregulated modulator of apoptosis) in variety of neuron death paradigms.. Activity of FoxO3a is governed by its post-translational modifications which control its sub-cellular localization.

Article Title: Smokeless tobacco induces toxicity and apoptosis in neuronal cells: a mechanistic evaluation.
Article Snippet: Smokeless tobacco (SLT) or chewing tobacco has been a highly addictive practice in India across ages, posing major threat to the systemic health and possibly neurodegeneration.. Earlier studies showed components of SLT could be harmful to neuronal health.. However, mechanism of SLT in neurodegeneration remained unexplored.

Article Title: The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.
Article Snippet: Accepted Manuscript The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas PII: S1044-7431(17)30370-6 DOI: doi:10.1016/j.mcn.2018.02.011 Reference: YMCNE 3287 To appear in: Molecular and Cellular Neuroscience Received date: 20 November 2017 Revised date: 23 February 2018 Accepted date: 25 February 2018 Please cite this article as: Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas , The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

Plasmid Preparation:

Article Title: Forkhead Box O3a requires BAF57, a subunit of chromatin remodeler SWI/SNF complex for induction of p53 up-regulated modulator of apoptosis (Puma) in a model of Parkinson's disease.
Article Snippet: Parkinson’s disease (PD) results from the selective loss of dopaminergic neurons of substantia nigra pars compacta region of the midbrain.. It has been reported that the transcription factor forkhead Box O3a (FoxO3a) is activated and induces pro-apoptotic protein such as BIM (Bcl-2 interacting mediator of cell death) and PUMA (p53 upregulated modulator of apoptosis) in variety of neuron death paradigms.. Activity of FoxO3a is governed by its post-translational modifications which control its sub-cellular localization.

Article Title: Smokeless tobacco induces toxicity and apoptosis in neuronal cells: a mechanistic evaluation.
Article Snippet: Smokeless tobacco (SLT) or chewing tobacco has been a highly addictive practice in India across ages, posing major threat to the systemic health and possibly neurodegeneration.. Earlier studies showed components of SLT could be harmful to neuronal health.. However, mechanism of SLT in neurodegeneration remained unexplored.

Article Title: The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.
Article Snippet: Accepted Manuscript The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas PII: S1044-7431(17)30370-6 DOI: doi:10.1016/j.mcn.2018.02.011 Reference: YMCNE 3287 To appear in: Molecular and Cellular Neuroscience Received date: 20 November 2017 Revised date: 23 February 2018 Accepted date: 25 February 2018 Please cite this article as: Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas , The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

Sequencing:

Article Title: Forkhead Box O3a requires BAF57, a subunit of chromatin remodeler SWI/SNF complex for induction of p53 up-regulated modulator of apoptosis (Puma) in a model of Parkinson's disease.
Article Snippet: Parkinson’s disease (PD) results from the selective loss of dopaminergic neurons of substantia nigra pars compacta region of the midbrain.. It has been reported that the transcription factor forkhead Box O3a (FoxO3a) is activated and induces pro-apoptotic protein such as BIM (Bcl-2 interacting mediator of cell death) and PUMA (p53 upregulated modulator of apoptosis) in variety of neuron death paradigms.. Activity of FoxO3a is governed by its post-translational modifications which control its sub-cellular localization.

Construct:

Article Title: Bcl-2–associated athanogene 3 protects the heart from ischemia/reperfusion injury
Article Snippet: .. Ad-GFP, Ad-BAG3, or Ad-siBAG3 was constructed using the BD Adeno-X Expression System, 2PT3674-1, and BD Knockout RNAi Systems, PT3739 (BD Biosciences-Clontech) as previously described ( 9 ). ..

Article Title: BAG3 regulates contractility and Ca 2+ homeostasis in adult mouse ventricular myocytes
Article Snippet: .. BAG3shRNA-Ad construct was made using the BD Adeno-X Expression Systems 2PT3674-1 (Pr36024) and BD knockout RNAi Systems PT3739 (PR42756)(BD Biosciences-Clontech, Palo Alto, CA) as previously described [ 15 ]. .. A dsDNA oligonucleotide against a specific BAG3 mRNA (5′-AAG GUU CAG ACC AUC UUG GAA-3′) was inserted in a RNAi-ready pSIREN-DNR vector designed to express a small hairpin RNA (shRNA) driven by the human Pol III-dependent U6 promoter.

Article Title: Cardiac Dysfunction in HIV-1 Transgenic Mouse: Role of Stress and BAG3
Article Snippet: .. BAG3shRNA‐Ad construct was made using the BD Adeno‐X Expression Systems 2PT3674‐1 (Pr36024) and BD knockout RNAi Systems PT3739 (PR42756)(BD Biosciences‐Clontech, Palo Alto, CA, USA) as previously described. ..

Transfection:

Article Title: The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.
Article Snippet: Accepted Manuscript The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas PII: S1044-7431(17)30370-6 DOI: doi:10.1016/j.mcn.2018.02.011 Reference: YMCNE 3287 To appear in: Molecular and Cellular Neuroscience Received date: 20 November 2017 Revised date: 23 February 2018 Accepted date: 25 February 2018 Please cite this article as: Rumana Akhter, Suraiya Saleem, Akash Saha, Subhas Chandra Biswas , The pro-apoptotic protein Bmf co-operates with Bim and Puma in neuron death induced by β-amyloid or NGF deprivation.. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

Functional Assay:

Article Title: Lrp4/corin dopamine-producing neuron precursor cell marker
Article Snippet: The nucleotide sequence of an siRNA can be designed using a computer program such as that disclosed at the Ambion website (http://www.ambion.com/techlib/misc/siRNA_finder.html). .. Kits for screening for functional siRNAs are also commercially available and can be used (for example, BD Knockout RNAi System (BD Biosciences Clontech). .. 228: 225; M. Koizumi and E. Ohtsuka (1990) Protein, Nucleic Acid and Enzyme 35: 2191; Koizumi et al. (1989) Nucleic Acids Res.



Similar Products

92
Eagle Biosciences total complement functional screen elisa kit
Total Complement Functional Screen Elisa Kit, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complement+functional+screen+elisa+kit/Total+complement+functional+screen+ELISA/pm37887754-49-11-17
Average 92 stars, based on 1 article reviews
total complement functional screen elisa kit - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier

92
Eagle Biosciences complement functional screen elisa kit
Complement Functional Screen Elisa Kit, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complement+functional+screen+elisa+kit/Total+complement+functional+screen+ELISA/pm37887754-49-12-17
Average 92 stars, based on 1 article reviews
complement functional screen elisa kit - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier

Image Search Results