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adiponectin  (Boster Bio)


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    Structured Review

    Boster Bio adiponectin
    Biochemical analysis of plasma from animals submitted to the experimental protocol.
    Adiponectin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+functional+screen+elisa+kit/Mouse+Adiponectin+ELISA+Kit+PicoKine/pmc11143230-290-14-16
    Average 93 stars, based on 28 article reviews
    adiponectin - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Consumption of interesterified palm oil leads inflammation of white adipose tissue and triggers metabolic disturbances in mice on a high-fat diet"

    Article Title: Consumption of interesterified palm oil leads inflammation of white adipose tissue and triggers metabolic disturbances in mice on a high-fat diet

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-63488-9

    Biochemical analysis of plasma from animals submitted to the experimental protocol.
    Figure Legend Snippet: Biochemical analysis of plasma from animals submitted to the experimental protocol.

    Techniques Used: Clinical Proteomics

    Palm oil and interesterified palm oil in a high-fat diet model caused morphofunctional changes in white adipose tissue. Hypertrophy resulting from the accumulation of lipids increases the production of inflammatory cytokines (IL-6, TNFα, MCP-1) and immune cells (F4-80), in addition to altering the leptin/adiponectin ratio, which is associated with metabolic dysregulation and the increased risk of chronic diseases. When the influx of fatty acids surpasses the storage capacity of adipose tissue, it leads to lipotoxicity and ectopic lipid accumulation. This accumulation promotes functional changes in the liver and pancreas. In liver tissue, lipogenesis (Srepb-1, Fas, Pparγ) is increased, whereas beta-oxidation (Cpt1-α, Pparα, Pgc1-α) is decreased, which further promotes lipid accumulation in the liver and can cause hepatic steatosis. In the pancreas, in addition to lipid accumulation, hyperglycemia caused by the diet causes pancreatic hypertrophy, with an increase in pancreatic mass (Ki-67) and insulin production, exacerbating the worsening of glucose metabolism. Therefore, the consumption of palm oil and interesterified palm oil in a high-fat diet model causes metabolic changes in the metabolism of carbohydrates and lipids, due to morpho-functional changes in adipose tissue and ectopic accumulation in other metabolic tissues (liver and pancreas).
    Figure Legend Snippet: Palm oil and interesterified palm oil in a high-fat diet model caused morphofunctional changes in white adipose tissue. Hypertrophy resulting from the accumulation of lipids increases the production of inflammatory cytokines (IL-6, TNFα, MCP-1) and immune cells (F4-80), in addition to altering the leptin/adiponectin ratio, which is associated with metabolic dysregulation and the increased risk of chronic diseases. When the influx of fatty acids surpasses the storage capacity of adipose tissue, it leads to lipotoxicity and ectopic lipid accumulation. This accumulation promotes functional changes in the liver and pancreas. In liver tissue, lipogenesis (Srepb-1, Fas, Pparγ) is increased, whereas beta-oxidation (Cpt1-α, Pparα, Pgc1-α) is decreased, which further promotes lipid accumulation in the liver and can cause hepatic steatosis. In the pancreas, in addition to lipid accumulation, hyperglycemia caused by the diet causes pancreatic hypertrophy, with an increase in pancreatic mass (Ki-67) and insulin production, exacerbating the worsening of glucose metabolism. Therefore, the consumption of palm oil and interesterified palm oil in a high-fat diet model causes metabolic changes in the metabolism of carbohydrates and lipids, due to morpho-functional changes in adipose tissue and ectopic accumulation in other metabolic tissues (liver and pancreas).

    Techniques Used: Functional Assay

    Related Articles

    Clinical Proteomics:

    Article Title: Consumption of interesterified palm oil leads inflammation of white adipose tissue and triggers metabolic disturbances in mice on a high-fat diet
    Article Snippet: Fasting blood glucose levels were measured using a glucometer (Accu-Chek; Roche, Germany). .. The plasma concentrations of insulin (EZRMI-13-K, Millipore, MO, USA), leptin (EZML-82K, Millipore, MO, USA), adiponectin (EK0596, Boster Biological Engineering Co., Ltd., Wuhan, China), interleukin 6 (IL-6, BMS603-2, Invitrogen, CA, USA), tumor necrosis factor (TNFα, #88-7324; Thermo Fisher Scientific, MA, USA), interleukin 1 beta (IL-1β #88-7013A-76, Thermo Fisher Scientific, MA, USA), glucagon-like peptide-1 (GLP-1, #BMS2194, Thermo Fisher Scientific, MA, USA) and glucose-dependent insulinotropic popypeptide (GIP, # EZRMGIP-55K, Millipore, MO, USA) were measured using mouse ELISA kits following the manufacturer's instructions. ..

    Article Title: Coffee consumption prevents obesity-related comorbidities and attenuates brown adipose tissue whitening in high-fat diet-fed mice.
    Article Snippet: This study aimed to evaluate the preventive and therapeutic effects of coffee consum ption on molecular changes and adipose tissue remodeling in a murine model of high-fat diet-induced obesity.. Three-month-old C57BL/6 mice were initially divided into three groups, namely, control (C), high-fat (HF), and coffee prevention (HF-CP) groups, and the HF group was subdivided at the end of the 10th week into two subgroups, an HF group and a coffee treatment (HF-CT) group; thus, a total of four groups were investigated at the 14th week of the experiment.. The HF-CP group had lower body mass than the HF group ( −7%, P < .05) and a better distribution of adipose tissue.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Consumption of interesterified palm oil leads inflammation of white adipose tissue and triggers metabolic disturbances in mice on a high-fat diet
    Article Snippet: Fasting blood glucose levels were measured using a glucometer (Accu-Chek; Roche, Germany). .. The plasma concentrations of insulin (EZRMI-13-K, Millipore, MO, USA), leptin (EZML-82K, Millipore, MO, USA), adiponectin (EK0596, Boster Biological Engineering Co., Ltd., Wuhan, China), interleukin 6 (IL-6, BMS603-2, Invitrogen, CA, USA), tumor necrosis factor (TNFα, #88-7324; Thermo Fisher Scientific, MA, USA), interleukin 1 beta (IL-1β #88-7013A-76, Thermo Fisher Scientific, MA, USA), glucagon-like peptide-1 (GLP-1, #BMS2194, Thermo Fisher Scientific, MA, USA) and glucose-dependent insulinotropic popypeptide (GIP, # EZRMGIP-55K, Millipore, MO, USA) were measured using mouse ELISA kits following the manufacturer's instructions. ..

    Article Title: Oleic acid influences the adipogenesis of 3T3-L1 cells via DNA Methylation and may predispose to obesity and obesity-related disorders.
    Article Snippet: .. Levels of adipokines were measured in a medium collected at D8 of differentiation using commercial kits: Mouse Adiponectin ELISA Research Reagent (Boster) for mouse adiponectin, the Mouse Leptin ELISA Kit (Sigma-Aldrich) for mouse leptin and Mouse IL-6 ELISA Kit (Millipore) for IL-6. .. The amount of accumulated lipids was measured using Oil Red O stain (Sigma-Aldrich).

    Article Title: Coffee consumption prevents obesity-related comorbidities and attenuates brown adipose tissue whitening in high-fat diet-fed mice.
    Article Snippet: This study aimed to evaluate the preventive and therapeutic effects of coffee consum ption on molecular changes and adipose tissue remodeling in a murine model of high-fat diet-induced obesity.. Three-month-old C57BL/6 mice were initially divided into three groups, namely, control (C), high-fat (HF), and coffee prevention (HF-CP) groups, and the HF group was subdivided at the end of the 10th week into two subgroups, an HF group and a coffee treatment (HF-CT) group; thus, a total of four groups were investigated at the 14th week of the experiment.. The HF-CP group had lower body mass than the HF group ( −7%, P < .05) and a better distribution of adipose tissue.

    Article Title: Liuwei Dihuang Decoction Alleviates Cognitive Dysfunction in Mice With D-Galactose-Induced Aging by Regulating Lipid Metabolism and Oxidative Stress via the Microbiota-Gut-Brain Axis
    Article Snippet: .. Vitamin E soft capsules (H20003539) were purchased from Zhejiang Pharmaceutical Co., Ltd. (Hangzhou, China); D-galactose (v900922) was purchased from Sigma–Aldrich (Shanghai, China); mouse adiponectin ELISA kit (EK0596) was purchased from Boster (Wuhan, China); mouse apolipoprotein E (ApoE) ELISA kit (ab215086) and mouse free fatty acid (FFA) assay kit (ab65341) were purchased from Abcam (Cambridge, United Kingdom); and superoxide dismutase (SOD) assay kit (20190412), glutathione peroxidase (GSH-Px) assay kit (20190309), and malondialdehyde (MDA) assay kit (20190315) were purchased from Jiancheng (Nanjing, China). ..

    Article Title: Consumption of phenolic-rich jabuticaba (Myrciaria jaboticaba) powder ameliorates obesity-related disorders in mice
    Article Snippet: Accumulating evidence indicates that dietary phenolic compounds can prevent obesity-related disorders.. We investigated whether the consumption of polyphenol-rich jabuticaba peel and seed powder (JPSP) could ameliorate the progression of diet-induced obesity in mice.. Male mice were fed a control diet or a high-fat (HF) diet for 9 weeks.

    Capsules:

    Article Title: Liuwei Dihuang Decoction Alleviates Cognitive Dysfunction in Mice With D-Galactose-Induced Aging by Regulating Lipid Metabolism and Oxidative Stress via the Microbiota-Gut-Brain Axis
    Article Snippet: .. Vitamin E soft capsules (H20003539) were purchased from Zhejiang Pharmaceutical Co., Ltd. (Hangzhou, China); D-galactose (v900922) was purchased from Sigma–Aldrich (Shanghai, China); mouse adiponectin ELISA kit (EK0596) was purchased from Boster (Wuhan, China); mouse apolipoprotein E (ApoE) ELISA kit (ab215086) and mouse free fatty acid (FFA) assay kit (ab65341) were purchased from Abcam (Cambridge, United Kingdom); and superoxide dismutase (SOD) assay kit (20190412), glutathione peroxidase (GSH-Px) assay kit (20190309), and malondialdehyde (MDA) assay kit (20190315) were purchased from Jiancheng (Nanjing, China). ..

    Malondialdehyde MDA Assay:

    Article Title: Liuwei Dihuang Decoction Alleviates Cognitive Dysfunction in Mice With D-Galactose-Induced Aging by Regulating Lipid Metabolism and Oxidative Stress via the Microbiota-Gut-Brain Axis
    Article Snippet: .. Vitamin E soft capsules (H20003539) were purchased from Zhejiang Pharmaceutical Co., Ltd. (Hangzhou, China); D-galactose (v900922) was purchased from Sigma–Aldrich (Shanghai, China); mouse adiponectin ELISA kit (EK0596) was purchased from Boster (Wuhan, China); mouse apolipoprotein E (ApoE) ELISA kit (ab215086) and mouse free fatty acid (FFA) assay kit (ab65341) were purchased from Abcam (Cambridge, United Kingdom); and superoxide dismutase (SOD) assay kit (20190412), glutathione peroxidase (GSH-Px) assay kit (20190309), and malondialdehyde (MDA) assay kit (20190315) were purchased from Jiancheng (Nanjing, China). ..



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