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human pluripotent stem cell functional identification kit  (R&D Systems)


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    R&D Systems human pluripotent stem cell functional identification kit
    Ethnically diverse parental fibroblasts and induced <t> Pluripotent </t> Stem Cell Lines.
    Human Pluripotent Stem Cell Functional Identification Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+functional+screen+elisa+kit/Human+Pluripotent+Stem+Cell+Functional+Identification+Kit/pmc05388175-109-23-30
    Average 97 stars, based on 141 article reviews
    human pluripotent stem cell functional identification kit - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines"

    Article Title: Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines

    Journal: Scientific Reports

    doi: 10.1038/srep15234

    Ethnically diverse parental fibroblasts and induced Pluripotent Stem Cell Lines.
    Figure Legend Snippet: Ethnically diverse parental fibroblasts and induced Pluripotent Stem Cell Lines.

    Techniques Used:

    ( a ) Schematic of the process flow to generate LTA-PDMS grids used for high throughput embryoid bodies (EBs) templating (top image), a PDMS mold of arrays prepared on a 4 inch wafer (middle), and 5 day time course of EBs formation in 200 μm LTA-PDMS grid wells (bottom image; (N > 400; scale bar is 200 μm). ( b ) EBs characterization by Hoechst, immunocytology for pluripotency markers Sox2 or SSEA-4, and Actin (phalloidin) staining. The F3.5.2 ED-iPSCs EBs shown was templated in a 200 μm well to day 5. Scale bar is 200 μm. ( c ) Representative images of differentiated 200 μm ED-iPSCs EBs using a Human pluripotent stem cell identification kit. Left column image shows bright field of pluripotent ED-iPSCs 2D colonies. Differentiated ED-iPSCs were fixed for ICC analysis at day 4 for the ectoderm and endoderm lineages and at day 3 for the mesoderm lineage. Antibodies used for immunocytology were indicated at the top of each column. Scale bars are 200 μm. ( d ) Colocalization of Hoescht nuclear stain and germ layer specific markers, listed in each image, to the nucleii of differentiated iPSC cells. Negative control is secondary antibody only. Representative images for germ layer commitment are shown for the iPSC line A2.2.2 at day 4 for ectoderm and endoderm and at day 3 for mesoderm. Scale bars are 200 μm.
    Figure Legend Snippet: ( a ) Schematic of the process flow to generate LTA-PDMS grids used for high throughput embryoid bodies (EBs) templating (top image), a PDMS mold of arrays prepared on a 4 inch wafer (middle), and 5 day time course of EBs formation in 200 μm LTA-PDMS grid wells (bottom image; (N > 400; scale bar is 200 μm). ( b ) EBs characterization by Hoechst, immunocytology for pluripotency markers Sox2 or SSEA-4, and Actin (phalloidin) staining. The F3.5.2 ED-iPSCs EBs shown was templated in a 200 μm well to day 5. Scale bar is 200 μm. ( c ) Representative images of differentiated 200 μm ED-iPSCs EBs using a Human pluripotent stem cell identification kit. Left column image shows bright field of pluripotent ED-iPSCs 2D colonies. Differentiated ED-iPSCs were fixed for ICC analysis at day 4 for the ectoderm and endoderm lineages and at day 3 for the mesoderm lineage. Antibodies used for immunocytology were indicated at the top of each column. Scale bars are 200 μm. ( d ) Colocalization of Hoescht nuclear stain and germ layer specific markers, listed in each image, to the nucleii of differentiated iPSC cells. Negative control is secondary antibody only. Representative images for germ layer commitment are shown for the iPSC line A2.2.2 at day 4 for ectoderm and endoderm and at day 3 for mesoderm. Scale bars are 200 μm.

    Techniques Used: High Throughput Screening Assay, Staining, Negative Control

    Related Articles

    Immunofluorescence:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Functional Assay:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Passaging:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Differentiation Assay:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Expressing:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    cDNA Synthesis:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Quantitative RT-PCR:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Incubation:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Blocking Assay:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate

    Marker:

    Article Title: Evaluating and comparing the signalling and functional landscape of hiPSC-CMs derived from patients with dilated cardiomyopathy.
    Article Snippet: min at RT, or with cold methanol:acetone (1:1) for 10minat−20 °C.Cellswerewashed3 timeswithPBSanduntil ready for immunofluorescence using the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, SC027B) to confirm presence of Otx2, Brachyury and Sox17, indicating successful differentiation of ectoderm, mesoderm and endoderm, respectively, as well as F-actin. hiPSCswere dissociate



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