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anti fh rabbit polyclonal  (Proteintech)


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    Structured Review

    Proteintech anti fh rabbit polyclonal
    Anti Fh Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+factor+h/Complement+factor+H+Antibody/pm41781748-285-17-20
    Average 88 stars, based on 4 article reviews
    anti fh rabbit polyclonal - by Bioz Stars, 2026-11
    88/100 stars

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    Related Articles

    Staining:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Negative Control:

    Article Title: Intracellular complement Factor H promotes tumor progression through modulation of cell cycle and actin cytoskeleton.
    Article Snippet: .. Cells were stained with 1:50 α-p53 mAb (SantaCruz sc126) or anti-FH OX24 (Mouse, expressed in house) or anti-FH rabbit polyclonal (Proteintech, ref 12748-1-AP) or isotype controls (Dako FLEX negative control mouse and rabbit cocktail, catalog no IR750 and IR600 respectively) overnight at 4°C, and then with Phalloidin-IFluor 488 reagent (Abcam ab176753) for 90 minutes in the dark. .. were used 70,71 at different concentrations ranging from 500 nM to 15.62 nM or 250 μM to 7.8 nM diluted in either normal or low ionic strength buffers.Data were analyzed using BIAevaluation software (version 4.1; Biacore, GE Healthcare) and the binding to the empty chip sensor surface was subtracted to estimate the kinetic binding constants.

    Flow Cytometry:

    Article Title: In Vitro Characterization of Derrone as an Aurora Kinase Inhibitor.
    Article Snippet: .. Flow cytometry and xCelligence system were performed in the Key Laboratory of Enzyme and Protein Technology facilities (HUS). ..

    Vaccines:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Control:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Membrane:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Binding Assay:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Recombinant:

    Article Title: Immunogenicity and safety of an investigational multicomponent, recombinant, meningococcal serogroup B vaccine (4CMenB) administered concomitantly with routine infant and child vaccinations: results of two randomised trials
    Article Snippet: Methods We did primary and booster phase 3 studies between March 31, 2008, and Aug 16, 2010, in 70 sites in Europe.. We used two series of sponsor-supplied, computer-generated randomisation envelopes to allocate healthy 2 month-old infants to receive routine vaccinations (diphtheria-tetanus-acellular pertussis, inactivated poliovirus, hepatitis B plus Haemophilus infl uenzae type b, and seven-valent pneumococcal vaccine) wat 2, 4, and 6 months of age alone, or concomitantly with 4CMenB or serogroup C conjugate vaccine (MenC) in: 1) an open-label, lot-to-lot immunogenicity and safety substudy of three 4CMenB lots compared with routine vaccines alone (1:1:1:1, block size eight); or 2) an observer-blind, lot-to-lot safety substudy of three 4CMenB lots compared with MenC (1:1:1:3, block size six).. At 12 months, 4CMenB-primed children from either substudy were randomised (1:1, block size two) to receive 4CMenB booster, with or without measles-mumps-rubella-varicella (MMRV) vaccine.

    Article Title: Multiplex colon cancer marker panel
    Article Snippet: Detection antibody enzyme Secondary antibody Substrate 11 Rabbit anti-human Anti-Rabbit IgG HRP HRP SF3B3 pAb4) (Invitrogen) chromogene 0.7 mg/ml 1/50000 12 Rabbit anti-human ALP Colorimetric GARS pAb4) ALP 0.4 mg/ml substrate 13 Rabbit anti-human Anti-Rabbit IgG HRP HRP ENO1 pAb (Invitrogen) chromogene (Proteintech Group) 1/50000 0.3 mg/ml 14 Mouse anti-human Anti-Mouse IgG HRP HRP RCN1 mAb (Abnova) (Invitrogen) chromogene 0.5 mg/ml 1/10000 15 TABLE 4 Gene No. .. Protein Name Name ELISA Kit Standard protein Capture antibody 16 Protein S100- S100A8/A9 Calprotectin, Human, A8/8100-S9 ELISA kit (Hycult biotechnology, Plymouth Meeting, PA) 17 Heat shock protein HSPB1 Hsp27 ELISA Kit beta-1 (Calbiochem, Darmstadt, Germany) 18 Receptor-type PTPRA Recombinant PTPRA Rabbit anti-human tyrosine-protein (SignalChem, British PTPRA pAb phosphatase alpha Columbia, Canada) (Proteintech Group) 3 mg/ml 19 Endoplasmin HSP90B1 Recombinant TRA1 Mouse anti-human (Abnova) TRA1 mAb (Proteintech Group) 5 mg/ml 20 Complement factor H CFH Native Factor H Sheep anti-human (AbD serotec, Factor H pAb (Gene Kidlington, UK) Tex, Irvine, CA) 5 μg/ml Conjugated No. .. Detection antibody enzyme Secondary antibody Substrate 16 17 18 Rabbit anti-human ALP Chemilumine PTPRA pAb4) scent ALP 1 mg/ml substrate 19 Rabbit anti-human Anti-Rabbit IgG ALP Chemilumine TRA1 pAb (Invitrogen) scent ALP (Proteintech Group) 1/20000 substrate 0.5 mg/ml 20 Sheep anti-human ALP Colorimetric Factor H pAb (Gene ALP Tex) substrate 0.5 μg/ml TABLE 5 Gene No.

    Knockdown:

    Article Title: Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling.
    Article Snippet: Four and a half LIM domains 1 (FHL1) belongs to a family of LIM-only proteins that regulate gene transcription, cell proliferation, differentiation and apoptosis.. However, the biological function of FHL1 remains largely unknown.. We used a yeast two-hybrid system and identified receptor interacting protein of 140 kDa (RIP140) as a novel FHL1-binding protein.

    Western Blot:

    Article Title: Four and a half LIM domains 1 (FHL1) and receptor interacting protein of 140kDa (RIP140) interact and cooperate in estrogen signaling.
    Article Snippet: Four and a half LIM domains 1 (FHL1) belongs to a family of LIM-only proteins that regulate gene transcription, cell proliferation, differentiation and apoptosis.. However, the biological function of FHL1 remains largely unknown.. We used a yeast two-hybrid system and identified receptor interacting protein of 140 kDa (RIP140) as a novel FHL1-binding protein.

    other:

    Article Title: Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter
    Article Snippet: FHL1 (rabbit) antibody was purchased from ProteinTech.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Multiplex colon cancer marker panel
    Article Snippet: Detection antibody enzyme Secondary antibody Substrate 11 Rabbit anti-human Anti-Rabbit IgG HRP HRP SF3B3 pAb4) (Invitrogen) chromogene 0.7 mg/ml 1/50000 12 Rabbit anti-human ALP Colorimetric GARS pAb4) ALP 0.4 mg/ml substrate 13 Rabbit anti-human Anti-Rabbit IgG HRP HRP ENO1 pAb (Invitrogen) chromogene (Proteintech Group) 1/50000 0.3 mg/ml 14 Mouse anti-human Anti-Mouse IgG HRP HRP RCN1 mAb (Abnova) (Invitrogen) chromogene 0.5 mg/ml 1/10000 15 TABLE 4 Gene No. .. Protein Name Name ELISA Kit Standard protein Capture antibody 16 Protein S100- S100A8/A9 Calprotectin, Human, A8/8100-S9 ELISA kit (Hycult biotechnology, Plymouth Meeting, PA) 17 Heat shock protein HSPB1 Hsp27 ELISA Kit beta-1 (Calbiochem, Darmstadt, Germany) 18 Receptor-type PTPRA Recombinant PTPRA Rabbit anti-human tyrosine-protein (SignalChem, British PTPRA pAb phosphatase alpha Columbia, Canada) (Proteintech Group) 3 mg/ml 19 Endoplasmin HSP90B1 Recombinant TRA1 Mouse anti-human (Abnova) TRA1 mAb (Proteintech Group) 5 mg/ml 20 Complement factor H CFH Native Factor H Sheep anti-human (AbD serotec, Factor H pAb (Gene Kidlington, UK) Tex, Irvine, CA) 5 μg/ml Conjugated No. .. Detection antibody enzyme Secondary antibody Substrate 16 17 18 Rabbit anti-human ALP Chemilumine PTPRA pAb4) scent ALP 1 mg/ml substrate 19 Rabbit anti-human Anti-Rabbit IgG ALP Chemilumine TRA1 pAb (Invitrogen) scent ALP (Proteintech Group) 1/20000 substrate 0.5 mg/ml 20 Sheep anti-human ALP Colorimetric Factor H pAb (Gene ALP Tex) substrate 0.5 μg/ml TABLE 5 Gene No.



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    Image Search Results


    FHR5 1-9 FH 1-5 binds to C3, destabilizes C3 convertase and acts as a cofactor for factor I. Binding profiles of increasing amounts of FHR5 1-9 FH 1-5 to surface bound C3, C3b, iC3b, C3d and BSA (negative control), detected with both anti-CFHR5 (a) and anti-FH (b) antibodies. Data shown are mean values of triplicate measurements; error bars denote the standard deviation. C3a generated by convertase formation on surface-bound C3b: ELISA plates were coated with purified C3b. Surface C3 convertase was formed by addition of purified factor B (FB), factor D (FD), and properdin (P). Then increasing amounts of either FH (c) or the FHR5 1-9 FH 1-5 (d) were added followed by addition of C3 to enable C3a generation. To investigate factor I (FI) co-factor activity, FI along with increasing amounts of either FH (e) or the FHR5 1-9 FH 1-5 (f) were added prior to the convertase formation step. NC—negative control, no FB, FD, or P added. PC—positive control, no FH, or FHR5 1-9 FH 1-5 added. P values were determined by one-way ANOVA with Dunnett’s multiple comparisons test; **** P < 0.0001. Fluid phase co-factor assay products were visualized by western blotting (g). Cofactor activity was indicated by the cleavage of C3b as determined by the presence of C3 α-chain fragments (arrow). Negative controls: C3, C3b, C3b with FH, C3b with FI, and FHR5 1-9 FH 1-5 alone. Positive control: C3b incubated with FH and FI.

    Journal: Clinical and Experimental Immunology

    Article Title: A novel fusion protein reduces kidney complement in experimental C3 glomerulopathy

    doi: 10.1093/cei/uxag015

    Figure Lengend Snippet: FHR5 1-9 FH 1-5 binds to C3, destabilizes C3 convertase and acts as a cofactor for factor I. Binding profiles of increasing amounts of FHR5 1-9 FH 1-5 to surface bound C3, C3b, iC3b, C3d and BSA (negative control), detected with both anti-CFHR5 (a) and anti-FH (b) antibodies. Data shown are mean values of triplicate measurements; error bars denote the standard deviation. C3a generated by convertase formation on surface-bound C3b: ELISA plates were coated with purified C3b. Surface C3 convertase was formed by addition of purified factor B (FB), factor D (FD), and properdin (P). Then increasing amounts of either FH (c) or the FHR5 1-9 FH 1-5 (d) were added followed by addition of C3 to enable C3a generation. To investigate factor I (FI) co-factor activity, FI along with increasing amounts of either FH (e) or the FHR5 1-9 FH 1-5 (f) were added prior to the convertase formation step. NC—negative control, no FB, FD, or P added. PC—positive control, no FH, or FHR5 1-9 FH 1-5 added. P values were determined by one-way ANOVA with Dunnett’s multiple comparisons test; **** P < 0.0001. Fluid phase co-factor assay products were visualized by western blotting (g). Cofactor activity was indicated by the cleavage of C3b as determined by the presence of C3 α-chain fragments (arrow). Negative controls: C3, C3b, C3b with FH, C3b with FI, and FHR5 1-9 FH 1-5 alone. Positive control: C3b incubated with FH and FI.

    Article Snippet: After incubation and washing, bound FHR5 1-9 FH 1-5 was detected using goat polyclonal anti-CFHR5 antibody (R&D systems), followed by mouse anti-goat/sheep IgG-HRP (Sigma) and finally TMB substrate (BD).

    Techniques: Binding Assay, Negative Control, Standard Deviation, Generated, Enzyme-linked Immunosorbent Assay, Purification, Activity Assay, Positive Control, Western Blot, Incubation