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human complement factor c3  (Cusabio)


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    Structured Review

    Cusabio human complement factor c3
    Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; <t>C3,</t> <t>complement</t> C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.
    Human Complement Factor C3, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+factor+3+(c3/Human+Complement+3%2CC3+ELISA+Kit/pm25017204-109-20-29
    Average 92 stars, based on 5 article reviews
    human complement factor c3 - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells."

    Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.

    Journal: Molecular medicine reports

    doi: 10.3892/mmr.2014.2376

    Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; C3, complement C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.
    Figure Legend Snippet: Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; C3, complement C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.

    Techniques Used: Microarray, Biomarker Discovery, Gene Expression, Real-time Polymerase Chain Reaction, Binding Assay

    Figure 4. Effects of SLC34A2 on C3, C4b production and Pi absorption of A549 cells. (A) Effect of SLC34A2 on C3 production of A549 cells was detected by ELISA assay at consecutive time points. (B) Effect of SLC34A2 on the C4b production of A549 cells was detected by an ELISA assay at consecutive time points. (C) Effect of SLC34A2 on the absorption of Pi in A549 cells was assayed by the phosphomolybdic acid method at consecutive time points. The values are expressed as the mean ± standard error of the mean of three independent sets of data. **P<0.01. C3, complement C3 precursor; C4b, complement 4B prepropro tein; SLC34A2, solute carrier family 34 (sodium phosphate), member 2.
    Figure Legend Snippet: Figure 4. Effects of SLC34A2 on C3, C4b production and Pi absorption of A549 cells. (A) Effect of SLC34A2 on C3 production of A549 cells was detected by ELISA assay at consecutive time points. (B) Effect of SLC34A2 on the C4b production of A549 cells was detected by an ELISA assay at consecutive time points. (C) Effect of SLC34A2 on the absorption of Pi in A549 cells was assayed by the phosphomolybdic acid method at consecutive time points. The values are expressed as the mean ± standard error of the mean of three independent sets of data. **P<0.01. C3, complement C3 precursor; C4b, complement 4B prepropro tein; SLC34A2, solute carrier family 34 (sodium phosphate), member 2.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Extracellular Vesicles Play a Central Role in Cerebral Venous Disease‐Associated Brain Atrophy
    Article Snippet: .. C3 levels were detected using an ELISA kit (CSB‐E08665h, Cusabio). .. After treatment with EVs from r‐DMV and c‐DMV, the level of soluble Aβ1‐40 and Aβ1‐42 in SH‐SY5Y cell pellets were detected using Aβ1‐40 (CSB‐E08299 h, Cusabio) and Aβ1‐42 (CSB‐E10684 h, Cusabio) ELISA kits.

    Article Title: Extracellular Vesicles Play a Central Role in Cerebral Venous Disease-Associated Brain Atrophy.
    Article Snippet: .. C3 levels were detected using an ELISA kit (CSB-E08665h, Cusabio). .. After treatment with EVs from r-DMV and c-DMV, the level of soluble Aβ1-40 and Aβ1-42 in SH-SY5Y cell pellets were detected using Aβ1-40 (CSB-E08299 h, Cusabio) and Aβ1-42 (CSB-E10684 h, Cusabio) ELISA kits.

    Article Title: Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals
    Article Snippet: .. C3d levels were detected in plasma using a Human complement C3 ELISA kit (Cusabio) according to manufacturer’s protocol. .. IL-1β and TNF- was detected in plasma and cell free supernatants using Human IL-1β DuoSet ELISA kit (R&D Systems) or Human TNF- DuoSet ELISA kit (R&D Systems) according to manufacturer’s protocol.

    Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.
    Article Snippet: .. Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34). .. Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34).

    Clinical Proteomics:

    Article Title: Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals
    Article Snippet: .. C3d levels were detected in plasma using a Human complement C3 ELISA kit (Cusabio) according to manufacturer’s protocol. .. IL-1β and TNF- was detected in plasma and cell free supernatants using Human IL-1β DuoSet ELISA kit (R&D Systems) or Human TNF- DuoSet ELISA kit (R&D Systems) according to manufacturer’s protocol.

    other:

    Article Title: Proteomic Identification of Biomarkers Associated with Eating Control and Bariatric Surgery Outcomes in Patients with Morbid Obesity.
    Article Snippet: The following ELISA kits were used: E-EL-H0038 and E-EL-H0848 (Elabscience, Wuhan, Hubei, China) for clusterin and complement factor B, respectively; KA1841 (Abnova, Taoyuan, Taiwan) for serum amyloid P protein; OKIA00064 (Aviva Systems Biology, San Diego, CA, USA) for haptoglobin; and CSB-E08665h (Cusabio, Houston, TX, USA) for complement factor C3.

    Concentration Assay:

    Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.
    Article Snippet: .. Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34). .. Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34).



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    Cusabio human complement factor c3
    Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; <t>C3,</t> <t>complement</t> C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.
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    Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; C3, complement C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.

    Journal: Molecular medicine reports

    Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.

    doi: 10.3892/mmr.2014.2376

    Figure Lengend Snippet: Figure 3. Differentially expressed genes between A549‑P‑S and A549‑P cells by microarray assay and qPCR validation. (A) Each group in triplicate was shown on the same chip. Color intensity was assigned to ratios of gene expression; shades of red indicate genes that were upregulated; shades of green indicate genes that were downregulated. (B) 10 upregulated genes were identified by qPCR, which was consistent with cDNA microarray data. qPCR, quantitative polymerase chain reaction; C3, complement C3 precursor; C4b, complement 4B preproprotein; FGA, fibrinogen α chain precursor; FGB, fibrinogen β chain precursor; FGG, fibrinogen γ chain precursor; SELENBP1, selenium binding protein 1; TXNIP, thioredoxin-interacting protein; PDZK1IP1, PDZK-interacting protein 1; DUSP6, dual specificity protein phosphatase 6.

    Article Snippet: Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34).

    Techniques: Microarray, Biomarker Discovery, Gene Expression, Real-time Polymerase Chain Reaction, Binding Assay

    Figure 4. Effects of SLC34A2 on C3, C4b production and Pi absorption of A549 cells. (A) Effect of SLC34A2 on C3 production of A549 cells was detected by ELISA assay at consecutive time points. (B) Effect of SLC34A2 on the C4b production of A549 cells was detected by an ELISA assay at consecutive time points. (C) Effect of SLC34A2 on the absorption of Pi in A549 cells was assayed by the phosphomolybdic acid method at consecutive time points. The values are expressed as the mean ± standard error of the mean of three independent sets of data. **P<0.01. C3, complement C3 precursor; C4b, complement 4B prepropro tein; SLC34A2, solute carrier family 34 (sodium phosphate), member 2.

    Journal: Molecular medicine reports

    Article Title: Elevated expression of SLC34A2 inhibits the viability and invasion of A549 cells.

    doi: 10.3892/mmr.2014.2376

    Figure Lengend Snippet: Figure 4. Effects of SLC34A2 on C3, C4b production and Pi absorption of A549 cells. (A) Effect of SLC34A2 on C3 production of A549 cells was detected by ELISA assay at consecutive time points. (B) Effect of SLC34A2 on the C4b production of A549 cells was detected by an ELISA assay at consecutive time points. (C) Effect of SLC34A2 on the absorption of Pi in A549 cells was assayed by the phosphomolybdic acid method at consecutive time points. The values are expressed as the mean ± standard error of the mean of three independent sets of data. **P<0.01. C3, complement C3 precursor; C4b, complement 4B prepropro tein; SLC34A2, solute carrier family 34 (sodium phosphate), member 2.

    Article Snippet: Then, the supernatants of each group of cells at 24, 48, 72 and 96 h were respectively collected to measure human complement factor C3 using the C3 ELISA kit (Cusabio, Wuhan, China) and C4b concentration using the C4b ELISA kit (Cusabio) according to the manufacturer's instructions (34).

    Techniques: Enzyme-linked Immunosorbent Assay