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decay accelerating factor for complement (cd55) antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology decay accelerating factor for complement (cd55) antibody
    Decay Accelerating Factor For Complement (Cd55) Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+decay-accelerating+factor+(cd55)/cd34+antibody/pmc01613263-256-35-43
    Average 90 stars, based on 1 article reviews
    decay accelerating factor for complement (cd55) antibody - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Staining:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Immunohistochemical staining:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Expressing:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Incubation:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Bioprocessing:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    shRNA:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Plasmid Preparation:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Inhibition:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Labeling:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Flow Cytometry:

    Article Title: The decay-accelerating factor (CD55) in acute leukemia patients and its query implication in cancer pathogenesis.
    Article Snippet: Customized CD55 shRNA plasmid (h) (sc-35012-SH, Santa Cruz Biotechnology, NC, USA) was used for the inhibition of CD55 expression in human cells.

    Article Title: Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells In Vitro .
    Article Snippet: The MSC-hBM were detached with a 2 mM EDTA solution, subsequently incubated for 30 min with anti-human monoclonal antibodies: HLA-DR (Santa Cruz Biotechnology, CA, USA), CD34 (Santa Cruz Biotechnology, CA, USA), CD45 (Thermo Scientific, USA), CD105 (Biolegend, USA), CD73 (Biolegend USA), and CD90 (Biolegend, USA), subsequently the cells were washed with PBS and centrifuged at 700 g min-1.

    Article Title: Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method.
    Article Snippet: Extended author information available on the last page of the article Abstract Mononuclear cells (MNCs), a type of leukocyte, require enrichment owing to their rarity for research and clinical applications.. The enrichment of MNCs is generally performed via conventional methods (e.g., density gradient centrifugation).. However, these methods have downsides, such as being labor intensive, energy and time consuming, and requiring advanced equipment.

    Article Title: Protocol for Obtaining Stem Cells from Menstrual Blood
    Article Snippet: MenSCs from two protocols were fixed in 4% paraformaldehyde (Merck, Cat. No: TP70404 415), and the distribution of MSC markers CD34 (Santa Cruz, Cat. No: sc-74499) and CD44 (Proteintech, Cat. No: 15675-1-AP) was analyzed using a biotin-streptavidin (Thermo Scientific, Cat. No: TP-125-HL) based on indirect immunoperoxidase staining protocol.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway.
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/106 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/106 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45PE (0.25 μg/106 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/106 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/106 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/106 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.

    Article Title: Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway
    Article Snippet: Well-grown passage 3 cells were resuspended for detection and backup, labeled with fluorescent antibodies: anti-CD34-FITC (0.25 μg/10 6 cells, Cat# sc-7324, Santa Cruz, CA, USA), anti-CD90-FITC (0.25 μg/10 6 cells, Cat# 202503, BioLegend, CA, USA), anti-CD45-PE (0.25 μg/10 6 cells, Cat# 202224, BioLegend, CA, USA), anti-CD29-PE (0.25 μg/10 6 cells, Cat# 102207, BioLegend, CA, USA), anti-RT1A-PE (0.25 μg/10 6 cells, Cat# 205208, BioLegend, USA), and anti-RT1B-FITC (0.25 μg/10 6 cells, Cat# 205305, BioLegend, CA, USA), and incubated for 30 min in the dark for flow cytometry.



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