Virus:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Recombinant:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Modification:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Produced:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Blocking Assay:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Incubation:
Article Title: HKU1 immune imprinting is associated with post-COVID symptoms after SARS-CoV-2 infection
Article Snippet: After 15 min of incubation at 37 °C, beads were pelleted and washed twice with 15 mM EDTA in PBS (Invitrogen, Thermo Fisher Scientific; cat# AM9260G). .. Beads were incubated with 50 μL of fluorescein-conjugated goat anti-guinea pig C3 antibody (MP Biomedicals, Irvine, CA, USA; cat# 0855385) for 15 min at room temperature, washed, resuspended in PBS, fixed with 4% PFA (Santa Cruz Biotechnology, Dallas, TX, USA; cat# sc-281692), and analyzed by flow cytometry. .. Data were processed using FlowJo software.
Article Title: Fc-engineered antibodies enhance protection against SARS-CoV-2 lung infection and inflammation.
Article Snippet: Fluorescence was acquired on the Intellicyt iQue, and phagocytic scores were determined specifically within the CD66b+ neutrophil population. .. After washing twice with PBS + 15 mM EDTA, beads were incubated with fluoresceinlabeled goat anti-guinea pig C3 (MP Biomedicals) for 30 min at room temperature. .. To evaluate ADCD, individual B.1.351 spike antigens were covalently linked to carboxylated Magplex beads by carbodiimide-NHS ester coupling (Thermo Fisher).
Staining:
Flow Cytometry:Article Title: HKU1 immune imprinting is associated with post-COVID symptoms after SARS-CoV-2 infection
Article Snippet: After 15 min of incubation at 37 °C, beads were pelleted and washed twice with 15 mM EDTA in PBS (Invitrogen, Thermo Fisher Scientific; cat# AM9260G). .. Beads were incubated with 50 μL of fluorescein-conjugated goat anti-guinea pig C3 antibody (MP Biomedicals, Irvine, CA, USA; cat# 0855385) for 15 min at room temperature, washed, resuspended in PBS, fixed with 4% PFA (Santa Cruz Biotechnology, Dallas, TX, USA; cat# sc-281692), and analyzed by flow cytometry. .. Data were processed using FlowJo software.
|
