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MyBiosource Biotechnology mouse complement fragment 4 d (c4 d) elisa kit
Mouse Complement Fragment 4 D (C4 D) Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complement+4+(c4/mouse+c3a+elisa+kit/pmc12081738-71-0-12
Average 90 stars, based on 1 article reviews
mouse complement fragment 4 d (c4 d) elisa kit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: An early HMGB1 rise 12 hours before creatinine predicts acute kidney injury and multiple organ failure in a smoke inhalation and burn swine model
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for analyzing HMGB1 (IBL International, Cat# 30164033), SDC-1 (Cloud-Clone Corporation, Cat# SEB966Po), and C3a (MyBioSource, Cat# MBS2509360) were purchased from commercial companies.

Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FITC-labelled hematopoietic lineage cocktail Thermo Fisher Scientific Ref#: 22-7770-72; RRID: AB_2644066 Anti-mouse C-kit (CD117) APC-eFluor780 (clone 2B8) Thermo Fisher Scientific Ref#: 47-1171-80; RRID: AB_1272213 Anti-mouse Sca1 (Ly6A/E) PE-Cy7 (clone J43) Thermo Fisher Scientific Ref#: 25-5981-81; RRID: AB_469668 Anti-mouse CD34 eFluor 450 (clone RAM34) Thermo Fisher Scientific Ref#: 48-0341-80; RRID: AB_2043838 Anti-mouse CD135 (Flt3) PerCP-eFlour 710 (clone A2F10) Thermo Fisher Scientific Ref#: 46-1351-80; RRID: AB_10733392 Anti-mouse IL7R (CD127) PE (clone A7R34) Thermo Fisher Scientific Ref#: 12-1271-82; RRID: AB_465844 Anti-human CFH (clone OX-24) Cedarlane Laboratories Ref#: AM20110PU-N; RRID: AB_10547520 Anti-mouse IgG1 kappa APC (clone P3.6.2.8.1) Thermo Fisher Scientific Ref#: 17-4714-82; RRID: AB_763649 Monoclonal anti-mouse CFH R&D Systems Ref#: MAB4999; RRID: AB_10890774 Polyclonal anti-mouse CFH R&D Systems Ref#: AF4999; RRID: AB_10972783 Anti-human C3 FITC MP BioMedicals Ref#: 0855500 Anti-mouse LC3 GeneTex Ref#: GTX127375; RRID: AB_11176277 Chemicals, peptides, and recombinant proteins Thioglycollate Thermo Fisher Scientific Ref#: CM0173B Zymosan Sigma Aldrich Ref#: Z4250 Bafilomycin A1 Eubio Ref#: BV1-0252-C100 4’,6-Diamidino-2-Phenylindole (DAPI) Sigma Aldrich Ref#: D1306 Cell Tracker Green CMFDA Thermo Fisher Scientific Ref#: C7025 Citrate buffer ph6.0, 10x Sigma Aldrich Ref#: C9999 Recombinant mouse CFH R&D Systems Ref#: 4999-FH pHrodo Red, succinimidyl ester Invitrogen Ref#: P36600 Purified mouse complement C3 Complement Technology Inc Ref#: M113 Critical commercial assays In Situ Cell Death Detection Kit TMR-Red Roche Diagnostics Ref#: 12156792910 Alexa Fluor 488 Antibody Labeling Kit Thermo Fisher Scientific Ref#: A20181 Foxp3/ Transcription Factor Staining Buffer set Thermo Fisher Scientific Ref#: 00-5523-00 Annexin V and 7-AAD viability solution kit BioLegend Ref#: 640934 Mouse C3 ELISA kit Abcam Ref#: ab263884 Mouse C5 ELISA kit Abcam Ref#: ab264609 Mouse C3a ELISA kit MyBioSource Ref#: MBS2701721 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Ref#: 23225 IL-1 beta Mouse Uncoated ELISA Kit Thermo Fisher Scientific Ref#: 88-7013-88 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Ref#: 4368814 Kapa SYBR Fast Bio-Rad iCycler with ROX dye Roche Diagnostics Ref#: KK4602 RNeasy mini kit Qiagen Ref#:74004 TruSeq Stranded mRNA LT sample preparation kit Illumina https://www.illumina.com (Continued on next page) e2 Immunity 56, 1809–1824.e1–e10, August 8, 2023

Enzyme-linked Immunosorbent Assay:

Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
Article Snippet: Adherent monocyte-derived macrophages (Mo-Macs) were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Invitrogen) and supplemented with increasing concentration of purified mouse complement C3 (Complement Technology Inc., Tyler, TX, USA). .. Consumption of added C3 was confirmed with quantifying cell-associated C3a levels using the Mouse C3a ELISA Kit (MyBioSource, San Diego, CA, USA). .. Protein loading was normalized in each lysate of adherent monocyte-derived macrophages using a Pearce BCA Protein Assay Kit (Thermo Fisher Scientific, Gibco).

Article Title: Efficacy of GalNAc C3 siRNAs in factor H-deficient mice with C3 glomerulopathy.
Article Snippet: .. Serum C3 levels were measured using a commercially available mouse C3 ELISA Kit (catalog MBS135930; MybioSource, San Diego, CA) according to the manufacturer’s instructions. ..

Article Title: Chronic rejection models for vascularized composite tissue allotransplantation
Article Snippet: All data were collected on an LSR Fortessa flow cytometer (BD Biosciences, San Jose, CA) and analyzed with WinList 9.0 software (Verity Software House, Topsham, ME). .. Mouse complement fragment 4 d (C4 d) ELISA kit was purchased from MyBioSource (San Diego, CA), and plasma was used to quantify levels of C4 d per the manufacturer’s instructions. ..

Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
Article Snippet: .. The concentration of complement components was determined by specific ELISA kits including the Mouse C3 ELISA kit (Abcam, Cambridge, UK), the Mouse C3a ELISA Kit e6 Immunity 56, 1809–1824.e1–e10, August 8, 2023 (MyBioSource, San Diego, CA, USA) and the Mouse C5 ELISA kit (Abcam, Cambridge, UK), according to the manufacturer’s recommendations. ..

Article Title: Liver factor B silencing to cure C3 glomerulopathy: Evidence from a mouse model of complement dysregulation.
Article Snippet: .. Serum C3 levels were measured using a commercially available mouse C3 ELISA kit (catalog MBS135930; MyBioSource, San Diego, CA, USA) according to the manufacturer’s instructions. ..

Clinical Proteomics:

Article Title: Chronic rejection models for vascularized composite tissue allotransplantation
Article Snippet: All data were collected on an LSR Fortessa flow cytometer (BD Biosciences, San Jose, CA) and analyzed with WinList 9.0 software (Verity Software House, Topsham, ME). .. Mouse complement fragment 4 d (C4 d) ELISA kit was purchased from MyBioSource (San Diego, CA), and plasma was used to quantify levels of C4 d per the manufacturer’s instructions. ..

Concentration Assay:

Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
Article Snippet: .. The concentration of complement components was determined by specific ELISA kits including the Mouse C3 ELISA kit (Abcam, Cambridge, UK), the Mouse C3a ELISA Kit e6 Immunity 56, 1809–1824.e1–e10, August 8, 2023 (MyBioSource, San Diego, CA, USA) and the Mouse C5 ELISA kit (Abcam, Cambridge, UK), according to the manufacturer’s recommendations. ..



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