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algorithms imagej n a n a photoshop n a n a prism graphpad n a n a cometscore 2 0 0 38 n a n a sequest software eng
Algorithms Imagej N A N A Photoshop N A N A Prism Graphpad N A N A Cometscore 2 0 0 38 N A N A Sequest Software Eng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 3 article reviews
Algorithms Imagej N A N A Photoshop N A N A Prism Graphpad N A N A Cometscore 2 0 0 38 N A N A Sequest Software Eng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cometscoring+software/pSB819-PKR-gib-SST+(Plasmid+%2320038)/pm40037355-256-161-157
Average 93 stars, based on 3 article reviews
algorithms imagej n a n a photoshop n a n a prism graphpad n a n a cometscore 2 0 0 38 n a n a sequest software eng - by Bioz Stars,
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Synthesized:Article Title: Localization of Fascin to Dendritic Protrusions Regulates Postsynaptic Plasticity Article Snippet: .. Custom single-stranded DNA oligonucleotides encoding guide RNAs (gRNAs) targeting the N-terminus (5’-TTGGCGGTCATGGTGGCGGA) of Fascin1 were synthesized, annealed, and subcloned into the Plasmid Preparation:Article Title: Localization of Fascin to Dendritic Protrusions Regulates Postsynaptic Plasticity Article Snippet: .. Custom single-stranded DNA oligonucleotides encoding guide RNAs (gRNAs) targeting the N-terminus (5’-TTGGCGGTCATGGTGGCGGA) of Fascin1 were synthesized, annealed, and subcloned into the Article Title: Structure, function, and evolution of the Orthobunyavirus membrane fusion glycoprotein. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. 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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-ET structure: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Halldorsson et al.49 RCSB PDB: 6f9c Previously available amino acid sequences GenBank see method details: Position- specific sequence similarity plots Experimental models: Cell lines Drosophila S2 Gibco/Thermo Fisher Scientific Cat# R690-07 BHK-21/BSR-T7/5 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0583 RK-13 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0109 SP2/0 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0223 Experimental models: Organisms/strains female BALB/c mice Specific pathogen-free breeding unit of the Friedrich-Loeffler-Institut, Germany N/A Recombinant DNA pCMV BAD C-HA vector Invitrogen/Thermo Fisher Scientific Cat# 82028 Software:Article Title: YTHDC1 cooperates with the THO complex to prevent RNA-damage-induced DNA breaks. Article Snippet: .. HeLa-S ATCC N/A Endogenously Flag-tagged YTHDC1 expressing HeLa-S This study N/A U2OS ATCC N/A U2OS 2-6-3 reporter cell Janicki et al.38 N/A HeLa ATCC N/A p53-deficient RPE-1 (parental) Shared by Dr. Martijn Luijsterburg (Leiden University Medical Center) N/A p53-deficient RPE-1 (XPG KO) Shared by Dr. Martijn Luijsterburg (Leiden University Medical Center) N/A p53-deficient RPE-1 (CSB KO) Shared by Dr. Martijn Luijsterburg (Leiden University Medical Center) N/A Recombinant DNA pET-28a-Flag-BsV-AlkB-NLS (WT) Tsao et al.15 N/A pET-28a-Flag-BsV-AlkB-NLS (H156A) Tsao et al.15 N/A pGEX-4T1-YTHDC1-YTH (WT) This study N/A pGEX-4T1-YTHDC1-YTH (W428A) This study N/A pHAGE-3xHA-ASCC2 Brickner et al.12 N/A pHAGE-3xHA-ALKBH3 Tsao et al.15 N/A pHAGE-Flag-TRMT61A (WT)-NLS Tsao et al.15 N/A pHAGE-Flag-TRMT61A (D181A)-NLS Tsao et al.15 N/A pMSCV-Flag-RNF113A Brickner et al.12 N/A pDD-Flag-TRMT61A (WT)-NLS-mCherry-LacI This study N/A pDD-Flag-TRMT61A (D181A)-NLS-mCherry-LacI This study N/A pDD-Flag-METTL1-NLS-mCherry-LacI This study N/A pHAGE-Flag-RNH (WT) This study N/A pHAGE-Flag-RNH (D210N) This study N/A pHAGE-Flag-YTHDC1 (WT) This study N/A pHAGE-Flag-YTHDC1 (W428A) This study N/A pGEX-6P-1-GST-MNase Addgene 136291 pGEX-6P-1-GST-RNase H1 D10R-E48R-MNase Addgene 136292 Software and Article Title: Structure, function, and evolution of the Orthobunyavirus membrane fusion glycoprotein. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-ET structure: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Halldorsson et al.49 RCSB PDB: 6f9c Previously available amino acid sequences GenBank see method details: Position- specific sequence similarity plots Experimental models: Cell lines Drosophila S2 Gibco/Thermo Fisher Scientific Cat# R690-07 BHK-21/BSR-T7/5 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0583 RK-13 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0109 SP2/0 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0223 Experimental models: Organisms/strains female BALB/c mice Specific pathogen-free breeding unit of the Friedrich-Loeffler-Institut, Germany N/A Recombinant DNA pCMV BAD C-HA vector Invitrogen/Thermo Fisher Scientific Cat# 82028 Tomography:Article Title: Structure, function, and evolution of the Orthobunyavirus membrane fusion glycoprotein. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-ET structure: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Halldorsson et al.49 RCSB PDB: 6f9c Previously available amino acid sequences GenBank see method details: Position- specific sequence similarity plots Experimental models: Cell lines Drosophila S2 Gibco/Thermo Fisher Scientific Cat# R690-07 BHK-21/BSR-T7/5 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0583 RK-13 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0109 SP2/0 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0223 Experimental models: Organisms/strains female BALB/c mice Specific pathogen-free breeding unit of the Friedrich-Loeffler-Institut, Germany N/A Recombinant DNA pCMV BAD C-HA vector Invitrogen/Thermo Fisher Scientific Cat# 82028 Virus:Article Title: Structure, function, and evolution of the Orthobunyavirus membrane fusion glycoprotein. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-ET structure: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Halldorsson et al.49 RCSB PDB: 6f9c Previously available amino acid sequences GenBank see method details: Position- specific sequence similarity plots Experimental models: Cell lines Drosophila S2 Gibco/Thermo Fisher Scientific Cat# R690-07 BHK-21/BSR-T7/5 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0583 RK-13 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0109 SP2/0 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0223 Experimental models: Organisms/strains female BALB/c mice Specific pathogen-free breeding unit of the Friedrich-Loeffler-Institut, Germany N/A Recombinant DNA pCMV BAD C-HA vector Invitrogen/Thermo Fisher Scientific Cat# 82028 Sequencing:Article Title: Structure, function, and evolution of the Orthobunyavirus membrane fusion glycoprotein. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-ET structure: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Halldorsson et al.49 RCSB PDB: 6f9c Previously available amino acid sequences GenBank see method details: Position- specific sequence similarity plots Experimental models: Cell lines Drosophila S2 Gibco/Thermo Fisher Scientific Cat# R690-07 BHK-21/BSR-T7/5 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0583 RK-13 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0109 SP2/0 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0223 Experimental models: Organisms/strains female BALB/c mice Specific pathogen-free breeding unit of the Friedrich-Loeffler-Institut, Germany N/A Recombinant DNA pCMV BAD C-HA vector Invitrogen/Thermo Fisher Scientific Cat# 82028 Membrane:Article Title: Structure, function, and evolution of the Orthobunyavirus membrane fusion glycoprotein. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LACV-GcF mAb 2A3 This study N/A anti-mouse Alexa Fluor 488 Invitrogen/Thermo Fisher Scientific RRID: AB_2534084, Cat# A-11017 anti-mouse Alexa Fluor 594 Invitrogen/Thermo Fisher Scientific RRID: AB_2534087, Cat# A-11020 Chemicals, peptides, and recombinant proteins 1:1 v/v mixture of paraffin oil and silicon oil Hampton Research Cat# HR3-413 Adjuvant MM GERBU Biotechnik Cat# 3001 Effectene transfection reagent Qiagen Cat# 301427 Xfect transfection reagent Takara Bio Cat# 631318 Insect-XPRESS protein-free medium with L-glutamine Lonza Cat# BE12-730Q penicillin-streptomycin Gibco Cat# 15140122 puromycin Invivogen Cat# ant-pr-1 polyethylene glycol 1500 Sigma-Aldrich Cat# P7306 Recombinant protein: LACV GcF (918–1364) W1066H mutant This study RCSB PDB: 7a57 Recombinant protein: LACV GcF (918–1345) This study N/A Recombinant protein: LACV GcF (927–1364) This study N/A Recombinant protein: LACV GcS (477–723) This study N/A Recombinant protein: LACV GcS (477–911) Hellert et al.42 RCSB PDB: 6h3w Recombinant protein: SBV GcF (881–1306) This study RCSB PDB: 7a56 Recombinant protein: SBV GcF (890–1326) This study N/A Critical commercial assays Organelle-LightsTM Golgi-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36215 Organelle-LightsTM ER-GFP BacMam 1.0 Invitrogen/Thermo Fisher Scientific Cat# O36212 Deposited data X-ray structure: La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a57 X-ray structure: Schmallenberg Virus Envelope Glycoprotein Gc Fusion Domains in Postfusion Conformation This study RCSB PDB: 7a56 X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form) Mishra et al.50 RCSB PDB: 7a5a X-ray structure: Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form) Mishra et al.50 RCSB PDB: 7a59 X-ray structure: CCHFV Gc prefusion monomer bound to ADI-36121 and ADI-37801 Fabs Mishra et al.50 RCSB PDB: 7l7r X-ray structure: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation Guardado-Calvo et al.48 RCSB PDB: 5ljz X-ray structure: Structure of RVFV envelope protein Gc in postfusion conformation in complex with 1,2-dipropionyl-sn-glycero-3-phosphocholine Guardado-Calvo et al.47 RCSB PDB: 6egu (Continued on next page) 16 Cell Reports 42, 112142, March 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-ET structure: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Halldorsson et al.49 RCSB PDB: 6f9c Previously available amino acid sequences GenBank see method details: Position- specific sequence similarity plots Experimental models: Cell lines Drosophila S2 Gibco/Thermo Fisher Scientific Cat# R690-07 BHK-21/BSR-T7/5 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0583 RK-13 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0109 SP2/0 Collection of Cell Lines in Veterinary Medicine, FLI, Germany Cat# CCLV-RIE 0223 Experimental models: Organisms/strains female BALB/c mice Specific pathogen-free breeding unit of the Friedrich-Loeffler-Institut, Germany N/A Recombinant DNA pCMV BAD C-HA vector Invitrogen/Thermo Fisher Scientific Cat# 82028 |