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A) Representative images of 53BP1 and γ-H2AX marked DNA DSBs in MCF10A cells 3-hours after mock treatment or treatment with10nM Estradiol (E2). B) Quantification of 53BP1 and γ-H2AX foci in cells described above. Bars represent mean number of foci per cell +/− SEM from three independent experiments (>200 cells were counted per experiment). Significance of changes in foci numbers were assessed using students two-tailed t-test C) Representative images of 53BP1 and γ-H2AX marked DNA DSBs in MCF10A cells 3-hours after mock treatment or treatment with1μM 2-OHE2 or 4-OHE2. D) Quantification of 53BP1 and γ-H2AX foci in cells described above. E) Representative comet images of MCF10A cells treated as above and subjected to neutral single cell electrophoresis. Fluorescence intensity mapping of comet images has been applied using <t>CometScore.</t> F) Quantification of tail DNA in comets from above. Tail DNA was quantified using CometScore. Bars represent mean percentage of DNA in comet tails, +/− SEM, from three independent experiments (>100 comets were scored per experiment). G) Frequency distribution of 53BP1 and γ-H2AX foci in MCF10A cells treated with 2-OHE2 or 4-OHE2 H) Representative images of γ-H2AX marked DNA DSBs in MCF10A cells 3-hours after mock treatment or treatment with 1μM 2-OHE2 or 4-OHE2. Cells were also stained with Cyclin-A antibodies to identify cells in S/G2-phase. I) Quantification of γ-H2AX foci in Cyclin-A positive and negative cells treated as above.

Cometscore, supplied by TriTek Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/comet+scoring+software+cometscore/cometscore/pmc04024319-74-4-5
Average 90 stars, based on 1 article reviews

cometscore - by Bioz Stars, 2026-10

90/100 stars

Images

1) Product Images from "BRCA1 DEFICIENCY EXACERBATES ESTROGEN INDUCED DNA DAMAGE AND GENOMIC INSTABILITY"

Article Title: BRCA1 DEFICIENCY EXACERBATES ESTROGEN INDUCED DNA DAMAGE AND GENOMIC INSTABILITY

Journal: Cancer research

doi: 10.1158/0008-5472.CAN-13-2611

Figure Legend Snippet: A) Representative images of 53BP1 and γ-H2AX marked DNA DSBs in MCF10A cells 3-hours after mock treatment or treatment with10nM Estradiol (E2). B) Quantification of 53BP1 and γ-H2AX foci in cells described above. Bars represent mean number of foci per cell +/− SEM from three independent experiments (>200 cells were counted per experiment). Significance of changes in foci numbers were assessed using students two-tailed t-test C) Representative images of 53BP1 and γ-H2AX marked DNA DSBs in MCF10A cells 3-hours after mock treatment or treatment with1μM 2-OHE2 or 4-OHE2. D) Quantification of 53BP1 and γ-H2AX foci in cells described above. E) Representative comet images of MCF10A cells treated as above and subjected to neutral single cell electrophoresis. Fluorescence intensity mapping of comet images has been applied using CometScore. F) Quantification of tail DNA in comets from above. Tail DNA was quantified using CometScore. Bars represent mean percentage of DNA in comet tails, +/− SEM, from three independent experiments (>100 comets were scored per experiment). G) Frequency distribution of 53BP1 and γ-H2AX foci in MCF10A cells treated with 2-OHE2 or 4-OHE2 H) Representative images of γ-H2AX marked DNA DSBs in MCF10A cells 3-hours after mock treatment or treatment with 1μM 2-OHE2 or 4-OHE2. Cells were also stained with Cyclin-A antibodies to identify cells in S/G2-phase. I) Quantification of γ-H2AX foci in Cyclin-A positive and negative cells treated as above.

Techniques Used: Two Tailed Test, Electrophoresis, Fluorescence, Staining

Microscopy:

Article Title: RETRACTED ARTICLE: Genotoxicity and alteration of the Gene Regulatory Network expression during Paracentrotus lividus development in the presence of carbon nanoparticles
Article Snippet: Slides were stained with ethidium bromide (20 μg/ ml) and analysed by Leica DM750 Fluorescence Microscope (Leica Microsystems GmbH, Wetzlar, Germany). .. Images were taken with a CCD Leica ICC50 HD camera coupled to an epifluorescence microscope and analysed with Comet Score TM (freeware from TriTek Corp., Summerduck, VA, USA). ..

Article Title: Protective effects of zinc L-carnosine against hydrogen peroxide-induced DNA damage and micronucleus formation in CCD-18co human colon fibroblast cells.
Article Snippet: Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=ifra20 Free Radical Research ISSN: 1071-5762 (Print) 1029-2470 (Online) Journal homepage: https://www.tandfonline.com/loi/ifra20 Protective effects of Zinc L-carnosine against hydrogen peroxide-induced DNA damage and micronucleus formation in CCD-18co human colon fibroblast cells Theng Choon Ooi, Kok Meng Chan & Razinah Sharif To cite this article: Theng Choon Ooi, Kok Meng Chan & Razinah Sharif (2020): Protective effects of Zinc L-carnosine against hydrogen peroxide-induced DNA damage and micronucleus formation in CCD-18co human colon fibroblast cells, Free Radical Research, DOI: 10.1080/10715762.2020.1763333 To link to this article: https://doi.org/10.1080/10715762.2020.1763333 Accepted author version posted online: 05 May 2020.. Submit your article to this journal View related articles View Crossmark data Protective effects of Zinc L-carnosine against hydrogen peroxideinduced DNA damage and micronucleus formation in CCD-18co human colon fibroblast cells.. Theng Choon Ooi a , Kok Meng Chan b and Razinah Sharif a,c * a Center for Healthy Ageing & Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. b Environmental Health and Industrial Safety Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. c Biocompatibility Laboratory, Centre for Research and Instrumentation, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia Theng Choon Ooi Email: ooithengchoon@ukm.edu.my Kok Meng Chan Email: chan@ukm.edu.my Razinah Sharif * Corresponding Author Address: Center for Healthy Ageing & Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.

Generated:

Article Title: Epigenetic reversal of hematopoietic stem cell aging in Phf6 knockout mice
Article Snippet: .. Analysis was performed on blinded files using CometScore (TriTek Corp), where values for percent tail DNA and Olive tail moment were generated. ..

Fluorescence:

other:

Article Title: G2 arrest primes hematopoietic stem cells for megakaryopoiesis
Article Snippet: Comet Score , TriTek Corp , .

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