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Trevigen comet analysis software version 1 0
Comet Analysis Software Version 1 0, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/comet+analysis+software/analysis+comet+software+trevigen/pmc12565254-192-17-22
Average 86 stars, based on 1 article reviews
comet analysis software version 1 0 - by Bioz Stars, 2026-09
86/100 stars

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Software:

Article Title: Comparative genotoxicity assessment of ortho-phthalaldehyde using human in vitro organotypic airway epithelial cultures and standard in vitro genotoxicity assays.
Article Snippet: ortho-Phthalaldehyde (OPA) is a high-level chemical disinfectant used for sterilizing heat-sensitive medical equipment.. OPA has been reported to be an airway irritant in animal studies, in human case reports, and in a human in vitro organotypic air–liquid-interface (ALI) airway culture model; there are also data indicating that it may be a direct-acting bacterial mutagen.. In this study, we investigated the genotoxic potential of OPA using human ALI airway cultures, human lymphoblastoid TK6 cells, and the Ames test.

Article Title: The Influence of TDP1 Inhibitor Usnic Acid Derivative OL9-116 on the Effects of Topotecan in Human Cells
Article Snippet: The slides were rinsed with cold water and stained with SYBR Green I (Thermo Fisher Scientific, USA). .. Images were acquired using a CELENA S digital microscope (Logos Bio-systems, Inc., Gunpo-si, Gyeonggi-do, Republic of Korea) and analyzed using comet assay analysis software version 1.0 (Trevigen, Inc., Gaithersburg, MD, USA). ..

Article Title: Reduced DNA repair capacity by lymphocytes in response to PM2.5 and wood smoke exposure predicts risk for lung cancer and COPD.
Article Snippet: Background: Susceptibility to lung cancer and COPD may reflect interindividual differences in DNA repair capacity (DRC) to complex airborne particulates.. Methods: Lymphocyte DRC was quantified in two high-risk smoker cohorts with an alkaline comet chip assay after exposure to total particulate matter (TPM) from PM2.5, wood smoke (WS), mainstream (MS) and sidestream (SS) cigarette smoke, and H2O2.. The micronucleus assay evaluated clastogenic response.

Article Title: Transferability and Reproducibility of the HepaRG CometChip Assay
Article Snippet: .. Images of all 96 wells were taken with a 4X objective and tiff files were analyzed using Trevigen Comet Analysis Software (version 1.3b). .. The Trevigen software automatically calculates percent tail DNA.

Article Title: Double strand breaks drive toxicity in a Huntington's disease mouse model with or without somatic expansion.
Article Snippet: 40 μL of this mixture was pipetted onto a 3-well FLARE slide (R&D Systems, Cat no. 3950-075-02) and spread with the pipette tip. .. Comet images were analyzed using Trevigen comet analysis software (R&D Systems, MN, USA). ..

Article Title: Transferability and Reproducibility of the HepaRG CometChip Assay.
Article Snippet: .. Images of all 96 wells were taken with a 4X objective and tiff files were analyzed using Trevigen Comet Analysis Software (version 1.3b). .. Images of all 96 wells were taken with a 4X objective and tiff files were analyzed using Trevigen Comet Analysis Software (version 1.3b).

Article Title: The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair
Article Snippet: Following 5 J m −2 UV-C irradiation, cells were further incubated with 4 mM HU and 40 μM AraC at 37°C for 0, 1, 2, and 4 h. CometChip gels were then covered with 1% LM Agarose (Trevigen, 4250-050-02) and incubated with lysis buffer (Trevigen, 4250-050-01) overnight at 4°C. .. Images were captured on a fluorescence microscope (Olympus, BX53), and the percentage of DNA in the tail was quantified using Comet analysis software (Trevigen, 42600-000-CS) [ ]. .. The chips were washed with 0.4 M Tris–HCl, pH 7.4 and stained with SYBR Gold (Thermo Fisher Scientific, S11494) according to the manufacturer’s protocol.

Article Title: Utilization of the CometChip assay for detecting PAH-induced DNA bulky adducts in a 3D primary human bronchial epithelial cell model
Article Snippet: Chips were imaged by a Keyence BZ-X710 Fluorescence Microscope (Keyence, Itasca, IL) using a Nikon Plan Fluor 10x/0.30∞/1.2 WD 15.2 objective lens (Keyence, Itasca, IL) taking 81 images per macro-well and using Image Merge BZ-XAnalyzer to stitch images into one image per macro-well. .. CometChip images were loaded into the Trevigen ® Comet Analysis Software (bio-techne ® R&D Systems, Minneapolis, MN) and scanned to identify useable comets. .. CometChip images were loaded into the Trevigen Comet Analysis Software (bio-techne R&D Systems, Minneapolis, MN) and scanned to identify useable comets.

Microscopy:

Article Title: The Influence of TDP1 Inhibitor Usnic Acid Derivative OL9-116 on the Effects of Topotecan in Human Cells
Article Snippet: The slides were rinsed with cold water and stained with SYBR Green I (Thermo Fisher Scientific, USA). .. Images were acquired using a CELENA S digital microscope (Logos Bio-systems, Inc., Gunpo-si, Gyeonggi-do, Republic of Korea) and analyzed using comet assay analysis software version 1.0 (Trevigen, Inc., Gaithersburg, MD, USA). ..

Article Title: The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair
Article Snippet: Following 5 J m −2 UV-C irradiation, cells were further incubated with 4 mM HU and 40 μM AraC at 37°C for 0, 1, 2, and 4 h. CometChip gels were then covered with 1% LM Agarose (Trevigen, 4250-050-02) and incubated with lysis buffer (Trevigen, 4250-050-01) overnight at 4°C. .. Images were captured on a fluorescence microscope (Olympus, BX53), and the percentage of DNA in the tail was quantified using Comet analysis software (Trevigen, 42600-000-CS) [ ]. .. The chips were washed with 0.4 M Tris–HCl, pH 7.4 and stained with SYBR Gold (Thermo Fisher Scientific, S11494) according to the manufacturer’s protocol.

Single Cell Gel Electrophoresis:

Article Title: The Influence of TDP1 Inhibitor Usnic Acid Derivative OL9-116 on the Effects of Topotecan in Human Cells
Article Snippet: The slides were rinsed with cold water and stained with SYBR Green I (Thermo Fisher Scientific, USA). .. Images were acquired using a CELENA S digital microscope (Logos Bio-systems, Inc., Gunpo-si, Gyeonggi-do, Republic of Korea) and analyzed using comet assay analysis software version 1.0 (Trevigen, Inc., Gaithersburg, MD, USA). ..

Fluorescence:

Article Title: The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair
Article Snippet: Following 5 J m −2 UV-C irradiation, cells were further incubated with 4 mM HU and 40 μM AraC at 37°C for 0, 1, 2, and 4 h. CometChip gels were then covered with 1% LM Agarose (Trevigen, 4250-050-02) and incubated with lysis buffer (Trevigen, 4250-050-01) overnight at 4°C. .. Images were captured on a fluorescence microscope (Olympus, BX53), and the percentage of DNA in the tail was quantified using Comet analysis software (Trevigen, 42600-000-CS) [ ]. .. The chips were washed with 0.4 M Tris–HCl, pH 7.4 and stained with SYBR Gold (Thermo Fisher Scientific, S11494) according to the manufacturer’s protocol.



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