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Epigenomics ag combinatorial barcodes
Characteristics of sample-multiplexing approaches used for single-cell sequencing
Combinatorial Barcodes, supplied by Epigenomics ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/combinatorial+barcoding/combinatorial+barcodes/pmc11073057-20-11-4
Average 90 stars, based on 1 article reviews
combinatorial barcodes - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Sample-multiplexing approaches for single-cell sequencing

Journal: Cellular and Molecular Life Sciences: CMLS

doi: 10.1007/s00018-022-04482-0

Characteristics of sample-multiplexing approaches used for single-cell sequencing
Figure Legend Snippet: Characteristics of sample-multiplexing approaches used for single-cell sequencing

Techniques Used: Membrane, Clinical Proteomics, Transfection, Transgenic Assay, shRNA, Polymerase Chain Reaction, DNA Methylation Assay, CRISPR

Schematic overview of five sample-multiplexing strategies used for scRNA-seq. A Natural genetic variation. Without additional labeling, computational demultiplexing is conducted based on SNPs. B Nucleotide-barcode anchoring on cellular or nuclear membranes. The example shown here is Cell Hashing, where oligo-tagged antibodies (hashtags) bind to ubiquitously expressed cell-surface proteins. Oligos with a poly (A) tail are captured along with mRNA. Cells can be assigned to their sample of origin based on different barcodes in the hashtags. C Nucleotide-barcode internalization into the cytoplasm or nucleus. Barcoded DNA traverses the cellular or nuclear membrane by liposomal transfection or directly diffuses into the nuclei. SBO: short barcode oligonucleotide. D Vector-based barcode expression in cells. E Nucleotide-barcode incorporation during library construction
Figure Legend Snippet: Schematic overview of five sample-multiplexing strategies used for scRNA-seq. A Natural genetic variation. Without additional labeling, computational demultiplexing is conducted based on SNPs. B Nucleotide-barcode anchoring on cellular or nuclear membranes. The example shown here is Cell Hashing, where oligo-tagged antibodies (hashtags) bind to ubiquitously expressed cell-surface proteins. Oligos with a poly (A) tail are captured along with mRNA. Cells can be assigned to their sample of origin based on different barcodes in the hashtags. C Nucleotide-barcode internalization into the cytoplasm or nucleus. Barcoded DNA traverses the cellular or nuclear membrane by liposomal transfection or directly diffuses into the nuclei. SBO: short barcode oligonucleotide. D Vector-based barcode expression in cells. E Nucleotide-barcode incorporation during library construction

Techniques Used: Multiplexing, Labeling, Membrane, Transfection, Plasmid Preparation, Expressing



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