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Epigenomics ag combinatorial barcodes
Characteristics of sample-multiplexing approaches used for single-cell sequencing
Combinatorial Barcodes, supplied by Epigenomics ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Sample-multiplexing approaches for single-cell sequencing

Journal: Cellular and Molecular Life Sciences: CMLS

doi: 10.1007/s00018-022-04482-0

Characteristics of sample-multiplexing approaches used for single-cell sequencing
Figure Legend Snippet: Characteristics of sample-multiplexing approaches used for single-cell sequencing

Techniques Used: Membrane, Clinical Proteomics, Transfection, Transgenic Assay, shRNA, Polymerase Chain Reaction, DNA Methylation Assay, CRISPR

Schematic overview of five sample-multiplexing strategies used for scRNA-seq. A Natural genetic variation. Without additional labeling, computational demultiplexing is conducted based on SNPs. B Nucleotide-barcode anchoring on cellular or nuclear membranes. The example shown here is Cell Hashing, where oligo-tagged antibodies (hashtags) bind to ubiquitously expressed cell-surface proteins. Oligos with a poly (A) tail are captured along with mRNA. Cells can be assigned to their sample of origin based on different barcodes in the hashtags. C Nucleotide-barcode internalization into the cytoplasm or nucleus. Barcoded DNA traverses the cellular or nuclear membrane by liposomal transfection or directly diffuses into the nuclei. SBO: short barcode oligonucleotide. D Vector-based barcode expression in cells. E Nucleotide-barcode incorporation during library construction
Figure Legend Snippet: Schematic overview of five sample-multiplexing strategies used for scRNA-seq. A Natural genetic variation. Without additional labeling, computational demultiplexing is conducted based on SNPs. B Nucleotide-barcode anchoring on cellular or nuclear membranes. The example shown here is Cell Hashing, where oligo-tagged antibodies (hashtags) bind to ubiquitously expressed cell-surface proteins. Oligos with a poly (A) tail are captured along with mRNA. Cells can be assigned to their sample of origin based on different barcodes in the hashtags. C Nucleotide-barcode internalization into the cytoplasm or nucleus. Barcoded DNA traverses the cellular or nuclear membrane by liposomal transfection or directly diffuses into the nuclei. SBO: short barcode oligonucleotide. D Vector-based barcode expression in cells. E Nucleotide-barcode incorporation during library construction

Techniques Used: Multiplexing, Labeling, Membrane, Transfection, Plasmid Preparation, Expressing

Related Articles

Polymerase Chain Reaction:

Article Title: Sample-multiplexing approaches for single-cell sequencing
Article Snippet: SPLiT-seq , 20180413 , Transcriptomics , mRNA , □ , Barcoded primers hybridize with 3’ mRNA , 48 , 1758; 156049 , 5498; 4497; 2055; 677 , Fixed cells and nuclei , NIH-3T3, HEK293, Hela-S3, Mouse brains and spinal cords , Single-cell combinatorial indexing (x-well plate) , [ 59 ]. .. sci-ATAC-seq , 20150522 , Epigenomics , Chromatin accessibility , □ , Combinatorial barcodes are introduced into DNA fragments by Tn5 and PCR , NA , >15000 , > 400 sites , Nuclei , GM12878, HL60, HEK293T , Single-cell combinatorial indexing (x-well plate) , [ 62 ]. .. dsciATAC-seq , 20190624 , Epigenomics , Chromatin accessibility , □ , Combinatorial barcodes are introduced into DNA fragments by Tn5 and PCR , 24; 96 , 510123 , ~ 100000 fragments per cell , Nuclei , GM12878, K562, NIH-3T3, Flash-frozen adult mouse whole-brain tissue, human BMMCs and PBMCs , Single-cell combinatorial indexing (x-well plate) and ddSEQ , [ 63 ].

Single Cell:

Article Title: Sample-multiplexing approaches for single-cell sequencing
Article Snippet: SPLiT-seq , 20180413 , Transcriptomics , mRNA , □ , Barcoded primers hybridize with 3’ mRNA , 48 , 1758; 156049 , 5498; 4497; 2055; 677 , Fixed cells and nuclei , NIH-3T3, HEK293, Hela-S3, Mouse brains and spinal cords , Single-cell combinatorial indexing (x-well plate) , [ 59 ]. .. sci-ATAC-seq , 20150522 , Epigenomics , Chromatin accessibility , □ , Combinatorial barcodes are introduced into DNA fragments by Tn5 and PCR , NA , >15000 , > 400 sites , Nuclei , GM12878, HL60, HEK293T , Single-cell combinatorial indexing (x-well plate) , [ 62 ]. .. dsciATAC-seq , 20190624 , Epigenomics , Chromatin accessibility , □ , Combinatorial barcodes are introduced into DNA fragments by Tn5 and PCR , 24; 96 , 510123 , ~ 100000 fragments per cell , Nuclei , GM12878, K562, NIH-3T3, Flash-frozen adult mouse whole-brain tissue, human BMMCs and PBMCs , Single-cell combinatorial indexing (x-well plate) and ddSEQ , [ 63 ].



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