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dna purification kit  (Jena Bioscience)


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    Structured Review

    Jena Bioscience dna purification kit
    Dna Purification Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/columns+kit/PCR+Purification+Kit+-+Column+Kit/pmc05779668-52-9-12
    Average 96 stars, based on 135 article reviews
    dna purification kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Identification of reproductive and molecular biomarkers associated with clinical metritis in dromedary camels ( Camelus dromedarius )
    Article Snippet: .. PCR amplicons exhibiting specific single bands were purified using a commercial PCR purification kit (Jena Bioscience, Germany) to eliminate residual primers and non-specific products ( ). ..

    Article Title: Molecular characterization of bovine viral diarrhea virus and host genetic immune and biochemical responses in diarrheic buffalo calves.
    Article Snippet: PCR results containing the target band were sent for forward and reverse DNA sequencing using an ABI 3730XL DNA sequencer (Applied Biosystem, USA) in order to identify SNPs in genes examined in control and diarrhea -affected calves. .. As described by Fonseca-Benitez et al.125, PCR purification kit was used in accordance with the manufacturer’s instructions to purify real-time PCR products with the desired size (target bands) (Jena Bioscience # pp-201×s/ Germany). .. The Nanodrop (Uv-Vis spectrophotometer Q5000/USA) was used to quantify the PCR product in order to produce high-quality results and guarantee sufficient concentrations and purity of the PCR products126.

    Article Title: Nucleotide sequence variations, gene expression profiles, and host biomarkers associated with ovine mange caused by Sarcoptes and Psoroptes mites: implications for diagnosis and disease control
    Article Snippet: .. Real-time PCR products displaying the expected fragment size were purified using a commercial PCR purification kit (Jena Bioscience, Germany; Cat. No. PP-201×s) according to the manufacturer’s instructions, following the principles described for nucleic acid purification protocols ( ). .. The concentration and purity of the purified amplicons were evaluated using a NanoDrop UV–Vis spectrophotometer (Q5000, USA) to confirm the adequate DNA quantity and optimal absorbance ratios required for sequencing analysis.

    Article Title: DNA barcode characteristics of Paphiopedilum supardii Braem & Loeb
    Article Snippet: .. Purification was performed using a Purification Kit (Jena Bioscience) directly from the PCR product (not from an agarose gel), as the separated PCR product showed a single DNA band of the target size. ..

    Article Title: Molecular characterization of bovine viral diarrhea virus and host genetic immune and biochemical responses in diarrheic buffalo calves
    Article Snippet: .. As described by Fonseca-Benitez et al. , PCR purification kit was used in accordance with the manufacturer’s instructions to purify real-time PCR products with the desired size (target bands) (Jena Bioscience # pp-201×s/Germany). .. The Nanodrop (Uv-Vis spectrophotometer Q5000/USA) was used to quantify the PCR product in order to produce high-quality results and guarantee sufficient concentrations and purity of the PCR products .

    Purification:

    Article Title: Identification of reproductive and molecular biomarkers associated with clinical metritis in dromedary camels ( Camelus dromedarius )
    Article Snippet: .. PCR amplicons exhibiting specific single bands were purified using a commercial PCR purification kit (Jena Bioscience, Germany) to eliminate residual primers and non-specific products ( ). ..

    Article Title: Molecular characterization of bovine viral diarrhea virus and host genetic immune and biochemical responses in diarrheic buffalo calves.
    Article Snippet: PCR results containing the target band were sent for forward and reverse DNA sequencing using an ABI 3730XL DNA sequencer (Applied Biosystem, USA) in order to identify SNPs in genes examined in control and diarrhea -affected calves. .. As described by Fonseca-Benitez et al.125, PCR purification kit was used in accordance with the manufacturer’s instructions to purify real-time PCR products with the desired size (target bands) (Jena Bioscience # pp-201×s/ Germany). .. The Nanodrop (Uv-Vis spectrophotometer Q5000/USA) was used to quantify the PCR product in order to produce high-quality results and guarantee sufficient concentrations and purity of the PCR products126.

    Article Title: Nucleotide sequence variations, gene expression profiles, and host biomarkers associated with ovine mange caused by Sarcoptes and Psoroptes mites: implications for diagnosis and disease control
    Article Snippet: .. Real-time PCR products displaying the expected fragment size were purified using a commercial PCR purification kit (Jena Bioscience, Germany; Cat. No. PP-201×s) according to the manufacturer’s instructions, following the principles described for nucleic acid purification protocols ( ). .. The concentration and purity of the purified amplicons were evaluated using a NanoDrop UV–Vis spectrophotometer (Q5000, USA) to confirm the adequate DNA quantity and optimal absorbance ratios required for sequencing analysis.

    Article Title: DNA barcode characteristics of Paphiopedilum supardii Braem & Loeb
    Article Snippet: .. Purification was performed using a Purification Kit (Jena Bioscience) directly from the PCR product (not from an agarose gel), as the separated PCR product showed a single DNA band of the target size. ..

    Article Title: Molecular characterization of bovine viral diarrhea virus and host genetic immune and biochemical responses in diarrheic buffalo calves
    Article Snippet: .. As described by Fonseca-Benitez et al. , PCR purification kit was used in accordance with the manufacturer’s instructions to purify real-time PCR products with the desired size (target bands) (Jena Bioscience # pp-201×s/Germany). .. The Nanodrop (Uv-Vis spectrophotometer Q5000/USA) was used to quantify the PCR product in order to produce high-quality results and guarantee sufficient concentrations and purity of the PCR products .

    Article Title: DNA barcoding and <i>COI</i> -based phylogenetic placement of <i>Ellochelon vaigiensis</i> (Mugiliformes: Mugilidae) from the Vietnamese Mekong Delta
    Article Snippet: The quality of PCR product was checked by electrophoresis on a 1.5% TBE agarose gel at 110 V for 40 min. A DL2000 DNA marker (Phusa company, Vietnam) was used to estimate PCR product size. .. The successfully amplified product was purified using a Purification Kit (Jena Bioscience) according to the manufacturer’s directions. .. Using the method of Sanger et al. (1977), the purified PCR product was sequenced on an ABI 3500 (Thermo Fisher) at PhuSa Biochem LTD, Can Tho City, Vietnam.

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular characterization of bovine viral diarrhea virus and host genetic immune and biochemical responses in diarrheic buffalo calves.
    Article Snippet: PCR results containing the target band were sent for forward and reverse DNA sequencing using an ABI 3730XL DNA sequencer (Applied Biosystem, USA) in order to identify SNPs in genes examined in control and diarrhea -affected calves. .. As described by Fonseca-Benitez et al.125, PCR purification kit was used in accordance with the manufacturer’s instructions to purify real-time PCR products with the desired size (target bands) (Jena Bioscience # pp-201×s/ Germany). .. The Nanodrop (Uv-Vis spectrophotometer Q5000/USA) was used to quantify the PCR product in order to produce high-quality results and guarantee sufficient concentrations and purity of the PCR products126.

    Article Title: Nucleotide sequence variations, gene expression profiles, and host biomarkers associated with ovine mange caused by Sarcoptes and Psoroptes mites: implications for diagnosis and disease control
    Article Snippet: .. Real-time PCR products displaying the expected fragment size were purified using a commercial PCR purification kit (Jena Bioscience, Germany; Cat. No. PP-201×s) according to the manufacturer’s instructions, following the principles described for nucleic acid purification protocols ( ). .. The concentration and purity of the purified amplicons were evaluated using a NanoDrop UV–Vis spectrophotometer (Q5000, USA) to confirm the adequate DNA quantity and optimal absorbance ratios required for sequencing analysis.

    Article Title: Molecular characterization of bovine viral diarrhea virus and host genetic immune and biochemical responses in diarrheic buffalo calves
    Article Snippet: .. As described by Fonseca-Benitez et al. , PCR purification kit was used in accordance with the manufacturer’s instructions to purify real-time PCR products with the desired size (target bands) (Jena Bioscience # pp-201×s/Germany). .. The Nanodrop (Uv-Vis spectrophotometer Q5000/USA) was used to quantify the PCR product in order to produce high-quality results and guarantee sufficient concentrations and purity of the PCR products .

    Nucleic Acid Purification:

    Article Title: Nucleotide sequence variations, gene expression profiles, and host biomarkers associated with ovine mange caused by Sarcoptes and Psoroptes mites: implications for diagnosis and disease control
    Article Snippet: .. Real-time PCR products displaying the expected fragment size were purified using a commercial PCR purification kit (Jena Bioscience, Germany; Cat. No. PP-201×s) according to the manufacturer’s instructions, following the principles described for nucleic acid purification protocols ( ). .. The concentration and purity of the purified amplicons were evaluated using a NanoDrop UV–Vis spectrophotometer (Q5000, USA) to confirm the adequate DNA quantity and optimal absorbance ratios required for sequencing analysis.

    Agarose Gel Electrophoresis:

    Article Title: DNA barcode characteristics of Paphiopedilum supardii Braem &amp;amp; Loeb
    Article Snippet: .. Purification was performed using a Purification Kit (Jena Bioscience) directly from the PCR product (not from an agarose gel), as the separated PCR product showed a single DNA band of the target size. ..

    other:

    Article Title: DNA barcoding and <i>COI</i> -based phylogenetic placement of <i>Ellochelon vaigiensis</i> (Mugiliformes: Mugilidae) from the Vietnamese Mekong Delta
    Article Snippet: The quality of PCR product was checked by electrophoresis on a 1.5% TBE agarose gel at 110 V for 40 min. A DL2000 DNA marker (Phusa company, Vietnam) was used to estimate PCR product size.

    Amplification:

    Article Title: DNA barcoding and <i>COI</i> -based phylogenetic placement of <i>Ellochelon vaigiensis</i> (Mugiliformes: Mugilidae) from the Vietnamese Mekong Delta
    Article Snippet: The quality of PCR product was checked by electrophoresis on a 1.5% TBE agarose gel at 110 V for 40 min. A DL2000 DNA marker (Phusa company, Vietnam) was used to estimate PCR product size. .. The successfully amplified product was purified using a Purification Kit (Jena Bioscience) according to the manufacturer’s directions. .. Using the method of Sanger et al. (1977), the purified PCR product was sequenced on an ABI 3500 (Thermo Fisher) at PhuSa Biochem LTD, Can Tho City, Vietnam.



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