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STEMCELL Technologies Inc column-based purification technique
Column Based Purification Technique, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/column+based+purification+methods/column+based+purification+technique/pmc02193277-58-9-12
Average 90 stars, based on 1 article reviews
column-based purification technique - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Persistence of HIV in gut-associated lymphoid tissue despite long-term antiretroviral therapy.
Article Snippet: CD4 T cells were isolated from PBMCs obtained from HIVinfected individuals, by use of a column-based cell separation technique (StemCell Technologies), as described elsewhere [24].

Article Title: Immune reconstitution in the sigmoid colon after long-term HIV therapy.
Article Snippet: CD4 + T cells were isolated from peripheral blood mononuclear cells using a column-based cell separation technique (StemCell Technologies, Vancouver, BC, Canada) as previously described.

Article Title: Effect of raltegravir intensification on HIV proviral DNA in the blood and gut mucosa of men on long-term therapy
Article Snippet: The amplification reaction was carried out in triplicate using 0.5 mmol/l primers, 0.2 mmol/l fluorescent probe, 0.8 mmol/l dNTPs, 5 mmmol/l MgCl2, and 2.5 U Platinum Taq Polymerase (Invitrogen) in 50 ml total volume.

Article Title: Can Probiotics Reduce Inflammation and Enhance Gut Immune Health in People Living with HIV: Study Designs for the Probiotic Visbiome for Inflammation and Translocation (PROOV IT) Pilot Trials.
Article Snippet: To cite this article: Connie J. Kim, Sharon L. Walmsley, Janet M. Raboud, Colin Kovacs, Bryan Coburn, Rodney Rousseau, Robert Reinhard, Ron Rosenes & Rupert Kaul (2016): Can Probiotics Reduce Inflammation and Enhance Gut Immune Health in People Living with HIV: Study Designs for the Probiotic Visbiome for Inflammation and Translocation (PROOV IT) Pilot Trials, HIV Clinical Trials, DOI: 10.1080/15284336.2016.1184827 To link to this article: http://dx.doi.org/10.1080/15284336.2016.1184827

Article Title: Sigmoid Th17 populations, the HIV latent reservoir, and microbial translocation in men on long-term antiretroviral therapy
Article Snippet: ISS Objective: Th17 cells play an important role in mucosal defence and repair and are highly susceptible to infection by HIV.. Antiretroviral therapy (ART) suppresses HIV viremia and can restore CD4þ numbers in the blood and gastrointestinal mucosa, but the resolution of systemic inflammation and gut microbial translocation is often incomplete.. We hypothesized that this might relate to persistent dysregulation of gut CD4þ Th17 subsets.

FACS:

Article Title: CXCR4 positive cells from Lewis lung carcinoma cell line have cancer metastatic stem cell characteristics.
Article Snippet: There is increasing evidence that cancer stem cells contribute to the initiation and propagation of many tumor.. Therefore, to find out and identify the metastatic tumor stem-like cells in Lewis lung cancer cell line (LLC), the expression of CXCR4 was measured in LLC by flow cytometry and observed by laser scanning confocal microscope (LSCM).. After the CXCR4 LLC cell was isolated from LLC by magnetic cell sorting, its properties were evaluated by their tumorigenic and metastatic potentials.

Purification:

Article Title: CXCR4 positive cells from Lewis lung carcinoma cell line have cancer metastatic stem cell characteristics.
Article Snippet: There is increasing evidence that cancer stem cells contribute to the initiation and propagation of many tumor.. Therefore, to find out and identify the metastatic tumor stem-like cells in Lewis lung cancer cell line (LLC), the expression of CXCR4 was measured in LLC by flow cytometry and observed by laser scanning confocal microscope (LSCM).. After the CXCR4 LLC cell was isolated from LLC by magnetic cell sorting, its properties were evaluated by their tumorigenic and metastatic potentials.

Magnetic Cell Separation:

Article Title: CXCR4 positive cells from Lewis lung carcinoma cell line have cancer metastatic stem cell characteristics.
Article Snippet: There is increasing evidence that cancer stem cells contribute to the initiation and propagation of many tumor.. Therefore, to find out and identify the metastatic tumor stem-like cells in Lewis lung cancer cell line (LLC), the expression of CXCR4 was measured in LLC by flow cytometry and observed by laser scanning confocal microscope (LSCM).. After the CXCR4 LLC cell was isolated from LLC by magnetic cell sorting, its properties were evaluated by their tumorigenic and metastatic potentials.

Suspension:

Article Title: Involvement of GPR43 Receptor in Effect of Lacticaseibacillus rhamnosus on Murine Steroid Resistant Chronic Obstructive Pulmonary Disease: Relevance to Pro-Inflammatory Mediators and Oxidative Stress in Human Macrophages
Article Snippet: .. Then, the cell suspension was added to the Separation Column (STEMCELL Technologies, Cambridge, UK) already properly connected to the magnetic support and with balanced electrical charges, waiting for the elution by gravity. ..



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