Review




Structured Review

FUJIFILM colorimetric kit based acs-acox method
A , body weight loss in the fasting rats. B , liver free fatty acids were remarkably increased in the fasting rats. C , liver TAG increased significantly in the fasting rats. D , gene expression of fatty acids ω-oxidation and peroxisomal β-oxidation in liver were upregulated by fasting. E , liver DC-CoA was significantly higher in fasting rats. F , liver peroxisomal β-oxidation was robustly induced in fasting rats. ABCD1, peroxisomal ATP-binding cassette transporter D; <t>ACOX1,</t> <t>acyl-CoA</t> <t>oxidase-1;</t> <t>ACS,</t> acyl-CoA synthetase; L-BP, L-bifunctional protein; LCADH, long-chain alcohol dehydrogenase; Thiolase, peroxisomal 3-oxoacyl-CoA thiolase. Mean ± SEM, n = 8, ∗ p < 0.05 by t -test between paired conditions.
Colorimetric Kit Based Acs Acox Method, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/colorimetric+kit+based+acs+acox+method/pmc08102918-258-11-13
Average 90 stars, based on 1 article reviews
colorimetric kit based acs-acox method - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation"

Article Title: Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2021.100622

A , body weight loss in the fasting rats. B , liver free fatty acids were remarkably increased in the fasting rats. C , liver TAG increased significantly in the fasting rats. D , gene expression of fatty acids ω-oxidation and peroxisomal β-oxidation in liver were upregulated by fasting. E , liver DC-CoA was significantly higher in fasting rats. F , liver peroxisomal β-oxidation was robustly induced in fasting rats. ABCD1, peroxisomal ATP-binding cassette transporter D; ACOX1, acyl-CoA oxidase-1; ACS, acyl-CoA synthetase; L-BP, L-bifunctional protein; LCADH, long-chain alcohol dehydrogenase; Thiolase, peroxisomal 3-oxoacyl-CoA thiolase. Mean ± SEM, n = 8, ∗ p < 0.05 by t -test between paired conditions.
Figure Legend Snippet: A , body weight loss in the fasting rats. B , liver free fatty acids were remarkably increased in the fasting rats. C , liver TAG increased significantly in the fasting rats. D , gene expression of fatty acids ω-oxidation and peroxisomal β-oxidation in liver were upregulated by fasting. E , liver DC-CoA was significantly higher in fasting rats. F , liver peroxisomal β-oxidation was robustly induced in fasting rats. ABCD1, peroxisomal ATP-binding cassette transporter D; ACOX1, acyl-CoA oxidase-1; ACS, acyl-CoA synthetase; L-BP, L-bifunctional protein; LCADH, long-chain alcohol dehydrogenase; Thiolase, peroxisomal 3-oxoacyl-CoA thiolase. Mean ± SEM, n = 8, ∗ p < 0.05 by t -test between paired conditions.

Techniques Used: Expressing, Binding Assay

A , kinetic parameters of key enzymes involved in peroxisomal and mitochondrial β-oxidation with C 12 -CoA and DC 12 -CoA as substrates. Mean ± SEM, n = 3. B , liver peroxisomal and mitochondrial β-oxidation activities with C 12 -CoA and DC 12 -CoA as a substrate respectively. ACOX1, acyl-CoA oxidase-1; CPT1, carnitine palmitoyltransferase-1; LCAD, long-chain acyl-CoA dehydrogenase; MCAD, medium-chain acyl-CoA dehydrogenase; SCAD, short-chain acyl-CoA dehydrogenase. Mean ± SEM, n = 6, ∗ p < 0.05 by t -test between paired conditions.
Figure Legend Snippet: A , kinetic parameters of key enzymes involved in peroxisomal and mitochondrial β-oxidation with C 12 -CoA and DC 12 -CoA as substrates. Mean ± SEM, n = 3. B , liver peroxisomal and mitochondrial β-oxidation activities with C 12 -CoA and DC 12 -CoA as a substrate respectively. ACOX1, acyl-CoA oxidase-1; CPT1, carnitine palmitoyltransferase-1; LCAD, long-chain acyl-CoA dehydrogenase; MCAD, medium-chain acyl-CoA dehydrogenase; SCAD, short-chain acyl-CoA dehydrogenase. Mean ± SEM, n = 6, ∗ p < 0.05 by t -test between paired conditions.

Techniques Used:

Related Articles

Concentration Assay:

Article Title: Long-chain dicarboxylic acids play a critical role in inducing peroxisomal β-oxidation and hepatic triacylglycerol accumulation
Article Snippet: .. Liver FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako). .. Plasma TAG was determined by commercial kit according to the manufacturer’s instructions (Wako).

Article Title: Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation
Article Snippet: .. Plasma FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako Pure Chemical Corporation, Osaka, Japan). .. Plasma TAG was determined by a commercial kit (Wako, Osaka, Japan).

Article Title: Peroxisomal β-oxidation stimulates cholesterol biosynthesis in the liver in diabetic mice
Article Snippet: .. Liver-FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako Pure Chemical Corporation). .. Plasma and liver total cholesterol were determined by a commercial kit from Sigma and performed according to the manufacturer’s instructions.

Article Title: Long-chain dicarboxylic acids play a critical role in inducing peroxisomal β-oxidation and hepatic triacylglycerol accumulation.
Article Snippet: .. Liver FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako). .. Plasma TAG was determined by commercial kit according to the manufacturer’s instructions (Wako).

Article Title: Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation
Article Snippet: .. Plasma FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako Pure Chemical Corporation, Osaka, Japan). .. Plasma TAG was determined by a commercial kit (Wako, Osaka, Japan).

Clinical Proteomics:

Article Title: Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation
Article Snippet: .. Plasma FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako Pure Chemical Corporation, Osaka, Japan). .. Plasma TAG was determined by a commercial kit (Wako, Osaka, Japan).

Article Title: Annona muricata leaf extract attenuates hepatic lipogenesis and adipogenesis.
Article Snippet: Annona muricata (graviola) is a medicinal plant that can be used to alleviate chronic human diseases by providing antioxidants and inducing immunomodulation.. In this study, we found that treatment of AML12 hepatocytes with steam (SGE) and ethanol (EGE) extracts of graviola leaf downregulated the expression of fatty acid (FA) oxidation genes, including ACOX1, CPT1, and PPARα, with no change in the expression of FA synthesis genes.. However, whereas EGE inhibited the differentiation and lipid accumulation of 3T3-L1 adipocytes and downregulated FA synthesis genes, no similar changes were observed in response to treatment with SGE.

Article Title: Fasting induces hepatic lipid accumulation by stimulating peroxisomal dicarboxylic acid oxidation
Article Snippet: .. Plasma FFA concentration was determined using a colorimetric kit based on ACS-ACOX method (Wako Pure Chemical Corporation, Osaka, Japan). .. Plasma TAG was determined by a commercial kit (Wako, Osaka, Japan).

Colorimetric Assay:

Article Title: Annona muricata leaf extract attenuates hepatic lipogenesis and adipogenesis.
Article Snippet: Annona muricata (graviola) is a medicinal plant that can be used to alleviate chronic human diseases by providing antioxidants and inducing immunomodulation.. In this study, we found that treatment of AML12 hepatocytes with steam (SGE) and ethanol (EGE) extracts of graviola leaf downregulated the expression of fatty acid (FA) oxidation genes, including ACOX1, CPT1, and PPARα, with no change in the expression of FA synthesis genes.. However, whereas EGE inhibited the differentiation and lipid accumulation of 3T3-L1 adipocytes and downregulated FA synthesis genes, no similar changes were observed in response to treatment with SGE.



Similar Products

86
Nanjing Jiancheng Bioengineering Research Institute Co Ltd titanium sulfate colorimetric method kit
Titanium Sulfate Colorimetric Method Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/colorimetric+kit+method+sulfate+titanium/pm42075366-175-7-38
Average 86 stars, based on 1 article reviews
titanium sulfate colorimetric method kit - by Bioz Stars, 2026-11
86/100 stars
  Buy from Supplier

97
Elabscience Biotechnology ion colorimetric method
Ion Colorimetric Method, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/Ferrous+Iron+Colorimetric+Assay+Kit/10__1016_slash_j__rbmo__2026__105633-123-17-21
Average 97 stars, based on 1 article reviews
ion colorimetric method - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier

95
Elabscience Biotechnology 733 direct method kits
733 Direct Method Kits, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/Low-density+Lipoprotein+Cholesterol+(LDL-C)+Colorimetric+Assay+Kit/pm41543766-277-10-14
Average 95 stars, based on 1 article reviews
733 direct method kits - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

95
Elabscience Biotechnology direct method kits
Direct Method Kits, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/Low-density+Lipoprotein+Cholesterol+(LDL-C)+Colorimetric+Assay+Kit/pmc12876478-240-9-12
Average 95 stars, based on 1 article reviews
direct method kits - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

95
Elabscience Biotechnology urease method
Urease Method, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/Urea+(BUN)+Colorimetric+Assay+Kit/10__4314_slash_tjpr__v25i9__4-49-14-16
Average 95 stars, based on 1 article reviews
urease method - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

94
Elabscience Biotechnology coomassie brilliant blue g 250 method
Coomassie Brilliant Blue G 250 Method, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/Bradford+Protein+Colorimetric+Assay+Kit/pm41232728-93-17-22
Average 94 stars, based on 1 article reviews
coomassie brilliant blue g 250 method - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

97
Elabscience Biotechnology bca method
Bca Method, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/BCA+Protein+Colorimetric+Assay+Kit/pm41102263-86-7-10
Average 97 stars, based on 1 article reviews
bca method - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier

99
Thermo Fisher colorimetric method kit
Colorimetric Method Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colorimetric+method+kit/Cholesterol/pm23978057__ja404714a_si_001-273-9-19
Average 99 stars, based on 1 article reviews
colorimetric method kit - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

Image Search Results