human sars coronavirus spike glycoprotein gene orf cdna (Sino Biological)

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Sino Biological human sars coronavirus spike glycoprotein gene orf cdna
Human Sars Coronavirus Spike Glycoprotein Gene Orf Cdna, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon/Human+SARS+coronavirus+(SARS-CoV)+Spike+glycoprotein+Gene+ORF+cDNA+clone+expression+plasmid+(Codon+Optimized)%2C+C-Flag+tag/us11634477-1148-7-28
Average 93 stars, based on 5 article reviews

human sars coronavirus spike glycoprotein gene orf cdna - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike
Article Snippet: The plasmids pCMV3-KLK13-HA (HG10199-CY), pCMV3-PCSK4 (HG21911-UT), pCMV3-SARS-CoV-1 spike-FLAG (VG40150-CF), and pCMV3-FLAG-HKU1 spike (VG40021-NF) were purchased from SinoBiological (Beijing, China).

Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike.
Article Snippet: The plasmids pCMV3-KLK13-HA (HG10199-CY), pCMV3-PCSK4 (HG21911-UT), pCMV3-SARS-CoV-1 spike-FLAG (VG40150CF), and pCMV3-FLAG-HKU1 spike (VG40021-NF) were purchased from SinoBiological (Beijing, China).

Expressing:

Article Title: Neutralizing anti-SARS-CoV-2 antibodies and methods of use thereof
Article Snippet: The S ORF was taken from “Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1” (NC_045512). .. For expression of full-length SARS-CoV S protein, “Human SARS coronavirus Spike glycoprotein Gene ORF cDNA clone expression plasmid (Codon Optimized)” (here referred to as pSARS—CoV-S) was obtained from SinoBiological (Cat: VG40150-G-N). .. A plasmid expressing a C-terminally truncated SARS-CoV-2 S protein (pSARS-CoV2-Strunc) was generated by insertion of a human-codon optimized cDNA encoding SARS-CoV-2 S lacking the C-terminal 19 codons (GENEART) into pCR3.1.

Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes
Article Snippet: SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-FLAG tag (Codon Optimized) , SinoBiological , VG40589-CF. .. Human SARS coronavirus (SARS-CoV) Spike glycoprotein Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tag , SinoBiological , VG40150-CF. .. Human coronavirus (HCoV-OC43) Spike Gene ORF cDNA clone expression plasmid(Codon Optimized), C-Flag tag , SinoBiological , VG40607-CF.

Article Title: Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells.
Article Snippet: .. The expression vectors coding for SARS- or MERS-CoV S with a C-terminal FLAG-tag, pCMV3-MERS-Spike-cFLAG (VG40069-CF) and pCMV3-SARS-Spike-cFLAG (VG40150-CF), as well as a vector encoding human dipeptidyl peptidase 4 (DPP4) with a C-terminal HA-tag, pCMV3-hDPP4-cHA (HG10688-CY), were purchased from Sino Biological, Houston, TX, USA. .. The pcDNA3.1(+) plasmid encoding human angiotensin-converting enzyme 2 (ACE2) with a C-terminal HA-tag was commercially obtained from GeneART (ThermoFisher).

Article Title: Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells
Article Snippet: .. The expression vectors coding for SARS- or MERS-CoV S with a C-terminal FLAG-tag, pCMV3-MERS-Spike-cFLAG (VG40069-CF) and pCMV3-SARS-Spike-cFLAG (VG40150-CF), as well as a vector encoding human dipeptidyl peptidase 4 (DPP4) with a C-terminal HA-tag, pCMV3-hDPP4-cHA (HG10688-CY), were purchased from Sino Biological, Houston, TX, USA. .. The pcDNA3.1(+) plasmid encoding human angiotensin-converting enzyme 2 (ACE2) with a C-terminal HA-tag was commercially obtained from GeneART (ThermoFisher).

Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes
Article Snippet: .. To quantify the levels of IgG binding various coronavirus spike proteins, 293T cells were transfected with SARS-CoV-2 (Sino Biological; VG40589-CF) (Genbank: YP_009724390.1 ), SARS-CoV-1 (Sino Biological; VG40150-CF) (Genbank: AAP13567.1), OC43 (Sino Biological; VG40607-CF) (Genbank: AVR40344.1), NL63 (Sino Biological; VG40604-CF) (Genbank: APF29071.1), or 229E (Sino Biological; VG40605-CF) (Genbank: APT69883.1) spike protein expression vectors. ..

Plasmid Preparation:

Binding Assay:

Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes
Article Snippet: .. To quantify the levels of IgG binding various coronavirus spike proteins, 293T cells were transfected with SARS-CoV-2 (Sino Biological; VG40589-CF) (Genbank: YP_009724390.1 ), SARS-CoV-1 (Sino Biological; VG40150-CF) (Genbank: AAP13567.1), OC43 (Sino Biological; VG40607-CF) (Genbank: AVR40344.1), NL63 (Sino Biological; VG40604-CF) (Genbank: APF29071.1), or 229E (Sino Biological; VG40605-CF) (Genbank: APT69883.1) spike protein expression vectors. ..

Transfection:

Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes
Article Snippet: .. To quantify the levels of IgG binding various coronavirus spike proteins, 293T cells were transfected with SARS-CoV-2 (Sino Biological; VG40589-CF) (Genbank: YP_009724390.1 ), SARS-CoV-1 (Sino Biological; VG40150-CF) (Genbank: AAP13567.1), OC43 (Sino Biological; VG40607-CF) (Genbank: AVR40344.1), NL63 (Sino Biological; VG40604-CF) (Genbank: APF29071.1), or 229E (Sino Biological; VG40605-CF) (Genbank: APT69883.1) spike protein expression vectors. ..

Review