expression plasmid sino biological (Sino Biological)
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Sino Biological
expression plasmid sino biological
Expression Plasmid Sino Biological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon/Human+coronavirus(HCoV-229E)+Spike+Gene+ORF+cDNA+clone+expression+plasmid(Codon+Optimized)%2C+C-Flag+tag/pmc07687490__mmc2-357-155-157
Average 93 stars, based on 9 article reviews
Expression Plasmid Sino Biological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon/Human+coronavirus(HCoV-229E)+Spike+Gene+ORF+cDNA+clone+expression+plasmid(Codon+Optimized)%2C+C-Flag+tag/pmc07687490__mmc2-357-155-157
Average 93 stars, based on 9 article reviews
expression plasmid sino biological - by Bioz Stars,
2026-10
93/100 stars
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Binding Assay:Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: .. To quantify the levels of IgG binding to various coronavirus spike proteins, 293T cells (ATTC, CRL-3216) were transfected with SARS-CoV-2 (Sino Biological, VG40589-CF) (GenBank YP_009724390.1), OC43 (Sino Biological, VG40607-CF) (GenBank AVR40344.1), or Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: .. To quantify the levels of IgG binding to various coronavirus spike proteins, 293T cells (ATTC, CRL-3216) were transfected with SARS-CoV-2 (Sino Biological, VG40589-CF) (GenBank YP_009724390.1), OC43 (Sino Biological, VG40607-CF) (GenBank AVR40344.1), or Transfection:Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: .. To quantify the levels of IgG binding to various coronavirus spike proteins, 293T cells (ATTC, CRL-3216) were transfected with SARS-CoV-2 (Sino Biological, VG40589-CF) (GenBank YP_009724390.1), OC43 (Sino Biological, VG40607-CF) (GenBank AVR40344.1), or Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: .. To quantify the levels of IgG binding to various coronavirus spike proteins, 293T cells (ATTC, CRL-3216) were transfected with SARS-CoV-2 (Sino Biological, VG40589-CF) (GenBank YP_009724390.1), OC43 (Sino Biological, VG40607-CF) (GenBank AVR40344.1), or Expressing:Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: .. To quantify the levels of IgG binding to various coronavirus spike proteins, 293T cells (ATTC, CRL-3216) were transfected with SARS-CoV-2 (Sino Biological, VG40589-CF) (GenBank YP_009724390.1), OC43 (Sino Biological, VG40607-CF) (GenBank AVR40344.1), or Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: .. To quantify the levels of IgG binding to various coronavirus spike proteins, 293T cells (ATTC, CRL-3216) were transfected with SARS-CoV-2 (Sino Biological, VG40589-CF) (GenBank YP_009724390.1), OC43 (Sino Biological, VG40607-CF) (GenBank AVR40344.1), or other:Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53 Article Snippet: The pcDNA3.1-puro empty vector was cloned by replacing the coding sequence of pcDNA3.1-puro Nodamura B2 (Addgene #17228) with the pcDNA3.1(+) multiple cloning site. Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Nutlin-3a Selleckchem S8059 Imatinib Sigma SML1027-10MG Berzosertib Selleckchem S7102 KU-55933 Selleckchem S1092 Temuterkib Selleckchem S8534 SCH772984 MedChemExpress HY-50846 25-hydroxycholesterol Sigma H1015-10MG Hemin chloride Cayman Chem 16487 Biotin-tyramide Apex Bio A8011 Lys-C Fisher Scientific NC9223464 Trypsin, mass spectrometry-grade Thermo 90305 SignalStain DAB Substrate Kit CST 8059 Critical commercial assays Renilla Luciferase Assay System Promega E2810 TMTpro 16plex Label Reagent Set Thermo A44520 TransIT-X2 Mirus MIR6000 KAPA mRNA HyperPrep kit Roche KK8580 Nano-Glo Live Cell Assay System Promega N2012 CellTiter-Glo Promega G7573 SuperScript IV Reverse Transcriptase kit Thermo 18090050 LightCycler 480 SYBR Green Roche 4887352001 Senescence beta-galactosidase activity assay CST 23833 QIAseq miRNA Library Kit Qiagen 331502 Proteome Profiler Human Cytokine Array Kit R&D Systems ARY005B Deposited data Raw ATAC-seq, RNA-seq, and small RNA-seq datasets This study GEO: GSE241893 Raw iDAPT-MS datasets This study ProteomeXchange: PXD034648 Experimental models: Cell lines HEK293T ATCC CRL-3216 VeroE6 ATCC CRL-1586 Huh7.5 ATCC CVCL-7927 Article Title: Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage Article Snippet: Recombinant DNA reagent , Article Title: Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-FLAG M2 Sigma Cat# F1804; RRID: AB_262044 Anti-DYKDDDDK (FLAG) Tag CST Cat# 14793S; RRID: AB_2572291 Anti-ACE2 ProSci Cat# 3217; RRID: AB_712925 Anti-ACTB Sigma Cat# A5316; RRID: AB_476743 Anti-SARS-CoV-2-Spike Mutagenesis:Article Title: Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Article Snippet: Masiulis et al.22 PDB: 6HUO MERS-CoV S structure in complex with 5-N-acetyl neuraminic acid Park et al.90 PDB: 6Q04 Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper PDB: 7YN0 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper PDB: 7YMZ Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper PDB: 7YMY Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper PDB: 7YMX Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper EMD-33949 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper EMD-33948 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper EMD-33947 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper EMD-33946 Fourier-cropped cryo-EM map of SARS-CoV-2 S-D614G (corresponding to PDB:7EAZ and EMDB:EMD-31047) This paper EMD-38650 Experimental models: Cell lines Expi293F Cells ThermoFisher Cat# A14527 HEK293 Freestyle ThermoFisher Cat# R79007 Recombinant DNA pcDNA3.1 CD33-N-cadherin (EC1-EC5; residues 160-712) This paper Custom synthesis based on Uniprot ID: NP_031690.3 pcDNA3.1 CD33-N-cadherin (EC4-EC5; residues 331-542) This paper Custom synthesis based on Uniprot ID: NP_031690.3 SARS-CoV-2 S protein with the D614G mutation in pcDNA3.4-TOPO This paper Custom synthesis based on Uniprot ID: P0DTC2 SARS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40150-G-N (GenBank ID: AAP13567.1) MERS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: APF29071.1) (Continued on next page) ll OPEN ACCESS Cell 187, 1296–1311.e1–e15, February 29, 2024 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER MERS-CoV S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: AFS88936.1) hCoV-NL63 S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40604-CF (GenBank ID: APF29071.1) hCoV-229E S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# Software:Article Title: Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Article Snippet: Masiulis et al.22 PDB: 6HUO MERS-CoV S structure in complex with 5-N-acetyl neuraminic acid Park et al.90 PDB: 6Q04 Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper PDB: 7YN0 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper PDB: 7YMZ Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper PDB: 7YMY Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper PDB: 7YMX Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper EMD-33949 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper EMD-33948 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper EMD-33947 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper EMD-33946 Fourier-cropped cryo-EM map of SARS-CoV-2 S-D614G (corresponding to PDB:7EAZ and EMDB:EMD-31047) This paper EMD-38650 Experimental models: Cell lines Expi293F Cells ThermoFisher Cat# A14527 HEK293 Freestyle ThermoFisher Cat# R79007 Recombinant DNA pcDNA3.1 CD33-N-cadherin (EC1-EC5; residues 160-712) This paper Custom synthesis based on Uniprot ID: NP_031690.3 pcDNA3.1 CD33-N-cadherin (EC4-EC5; residues 331-542) This paper Custom synthesis based on Uniprot ID: NP_031690.3 SARS-CoV-2 S protein with the D614G mutation in pcDNA3.4-TOPO This paper Custom synthesis based on Uniprot ID: P0DTC2 SARS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40150-G-N (GenBank ID: AAP13567.1) MERS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: APF29071.1) (Continued on next page) ll OPEN ACCESS Cell 187, 1296–1311.e1–e15, February 29, 2024 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER MERS-CoV S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: AFS88936.1) hCoV-NL63 S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40604-CF (GenBank ID: APF29071.1) hCoV-229E S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# Electron Microscopy:Article Title: Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Article Snippet: Masiulis et al.22 PDB: 6HUO MERS-CoV S structure in complex with 5-N-acetyl neuraminic acid Park et al.90 PDB: 6Q04 Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper PDB: 7YN0 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper PDB: 7YMZ Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper PDB: 7YMY Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper PDB: 7YMX Cryo-EM structure of MERS-CoV spike protein, all RBD-down conformation This paper EMD-33949 Cryo-EM structure of MERS-CoV spike protein, intermediate conformation This paper EMD-33948 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 1 This paper EMD-33947 Cryo-EM structure of MERS-CoV spike protein, One RBD-up conformation 2 This paper EMD-33946 Fourier-cropped cryo-EM map of SARS-CoV-2 S-D614G (corresponding to PDB:7EAZ and EMDB:EMD-31047) This paper EMD-38650 Experimental models: Cell lines Expi293F Cells ThermoFisher Cat# A14527 HEK293 Freestyle ThermoFisher Cat# R79007 Recombinant DNA pcDNA3.1 CD33-N-cadherin (EC1-EC5; residues 160-712) This paper Custom synthesis based on Uniprot ID: NP_031690.3 pcDNA3.1 CD33-N-cadherin (EC4-EC5; residues 331-542) This paper Custom synthesis based on Uniprot ID: NP_031690.3 SARS-CoV-2 S protein with the D614G mutation in pcDNA3.4-TOPO This paper Custom synthesis based on Uniprot ID: P0DTC2 SARS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40150-G-N (GenBank ID: AAP13567.1) MERS-CoV S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: APF29071.1) (Continued on next page) ll OPEN ACCESS Cell 187, 1296–1311.e1–e15, February 29, 2024 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER MERS-CoV S protein with the fm2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40069-NF (GenBank ID: AFS88936.1) hCoV-NL63 S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# VG40604-CF (GenBank ID: APF29071.1) hCoV-229E S protein with the 2P mutation in pcDNA3.4-TOPO This paper Subcloned from Sino Biological Cat# |