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Sangon Biotech codon optimized gene sequence
Codon Optimized Gene Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon/gene+sequence/pm42296048-193-1-11
Average 86 stars, based on 1 article reviews
codon optimized gene sequence - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Sequencing:

Article Title: Development of a novel high-affinity anti-NGAL monoclonal antibody pair for sensitive detection of acute kidney injury.
Article Snippet: Neutrophil Gelatinase-Associated Lipocalin (NGAL) is an early marker for Acute Kidney Injury (AKI) recommended by the International Society of Nephrology (ISN).. This study obtained recombinant NGAL protein via a prokaryotic expression system.. After immunizing BALB/c mice, four NGAL-specific monoclonal antibodies were screened using hybridoma technology.

Article Title: Molecular chaperone effects on recombinant yield and binding characteristics of an ABA-specific scFv in Escherichia coli
Article Snippet: The E. coli strain BL21 (DE3) was purchased from Sangon Biotech (China). .. The ABA-scFv gene sequence, referenced from , was synthesized by Sangon Biotech and cloned into the pET30a vector to create the expression plasmid pET30a-ABA-scFv . .. To establish the chaperone co-expression systems, BL21 (DE3) competent cells were first transformed with one of five molecular chaperone plasmids: pG-KJE8 , pGro7 , pKJE7 , pG-Tf2 , or pTf16 (Takara, Japan), as detailed in .

Article Title: A genetically programmable, conjugation-free nanomedicine platform for modular antibody-guided drug delivery
Article Snippet: .. The scFv-ALB fusion gene sequence was synthesized by Sangon Biotech and subcloned into the mammalian expression vector pX17.4 to construct the recombinant plasmid pX17.4-scFv-ALB (αHER2). ..

Article Title: Development of a Highly Sensitive ELISA for Detecting Antibodies Against a Novel Variant Avian Reovirus Based on Dual σC and σB Antigens.
Article Snippet: .. Based on the nucleotide sequence of the LY383 strain (GenBank accession number: AWV55509), the σC and σB gene sequences were synthesized by Sangon Biotech (Shanghai, China) and subsequently cloned into the pMD19T vector (Takara, Kyoto, Japan). ..

Article Title: Production method of recombinant
Article Snippet: .. Strain and Plasmid Used in the Present Invention For genes of the 7β-HSDH and the GDH, Sangon Biotech (Shanghai) Co., Ltd. performs a codon optimization and a complete gene synthesis on a gene sequence according to codon bias of E. coli. .. An E. coli BL21(DE3) strain is purchased from Sangon Biotech (Shanghai) Co., Ltd.

Article Title: An Autocatalytic Molecular Sensor Enables Rapid, Sensitive, and One-Pot Detection of Nucleic Acids.
Article Snippet: Circulating nucleic acids are emerging disease markers whose clinical applications are hindered by the lack of rapid, sensitive, convenient, and cost-effective detection assays.. Inspired by the natural replication of virus, here, we developed a biomimetic one-step, one-pot, isothermal detection assay named RAPID to realize rapid and sensitive detection of nucleic acids with minimal reliance on instruments.. The core element of RAPID is an autocatalytic molecular sensor that exploits the viral replication endonuclease of duck circovirus (i.e., DCV) to transform rolling circle amplification (RCA) from linear to exponential.

Article Title: High-level expression of Pandalus borealis duplex-specific nuclease by Komagataella phaffii via promoter and peptide selection
Article Snippet: Duplex-specific nuclease (DSN) is a necessary component to digest double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrid duplexes specifically, and widely used in food analysis and biological sample treatment.. However, few cases were reported to produce DSN harnessing genetic engineering, which limited the application and pushed up the cost of DSN.. In this study, Pandalus borealis DSN gene was expressed by the famous microbial host, Komagataella phaffii, through three steps including promoter and signal peptide selection, DSN gene expression cassette increasing, cultivation optimization to reach 2.6 × 105 U/μL in fermentation broth.

Synthesized:

Article Title: Development of a novel high-affinity anti-NGAL monoclonal antibody pair for sensitive detection of acute kidney injury.
Article Snippet: Neutrophil Gelatinase-Associated Lipocalin (NGAL) is an early marker for Acute Kidney Injury (AKI) recommended by the International Society of Nephrology (ISN).. This study obtained recombinant NGAL protein via a prokaryotic expression system.. After immunizing BALB/c mice, four NGAL-specific monoclonal antibodies were screened using hybridoma technology.

Article Title: Molecular chaperone effects on recombinant yield and binding characteristics of an ABA-specific scFv in Escherichia coli
Article Snippet: The E. coli strain BL21 (DE3) was purchased from Sangon Biotech (China). .. The ABA-scFv gene sequence, referenced from , was synthesized by Sangon Biotech and cloned into the pET30a vector to create the expression plasmid pET30a-ABA-scFv . .. To establish the chaperone co-expression systems, BL21 (DE3) competent cells were first transformed with one of five molecular chaperone plasmids: pG-KJE8 , pGro7 , pKJE7 , pG-Tf2 , or pTf16 (Takara, Japan), as detailed in .

Article Title: A genetically programmable, conjugation-free nanomedicine platform for modular antibody-guided drug delivery
Article Snippet: .. The scFv-ALB fusion gene sequence was synthesized by Sangon Biotech and subcloned into the mammalian expression vector pX17.4 to construct the recombinant plasmid pX17.4-scFv-ALB (αHER2). ..

Article Title: Development of a Highly Sensitive ELISA for Detecting Antibodies Against a Novel Variant Avian Reovirus Based on Dual σC and σB Antigens.
Article Snippet: .. Based on the nucleotide sequence of the LY383 strain (GenBank accession number: AWV55509), the σC and σB gene sequences were synthesized by Sangon Biotech (Shanghai, China) and subsequently cloned into the pMD19T vector (Takara, Kyoto, Japan). ..

Article Title: An Autocatalytic Molecular Sensor Enables Rapid, Sensitive, and One-Pot Detection of Nucleic Acids.
Article Snippet: Circulating nucleic acids are emerging disease markers whose clinical applications are hindered by the lack of rapid, sensitive, convenient, and cost-effective detection assays.. Inspired by the natural replication of virus, here, we developed a biomimetic one-step, one-pot, isothermal detection assay named RAPID to realize rapid and sensitive detection of nucleic acids with minimal reliance on instruments.. The core element of RAPID is an autocatalytic molecular sensor that exploits the viral replication endonuclease of duck circovirus (i.e., DCV) to transform rolling circle amplification (RCA) from linear to exponential.

Article Title: High-level expression of Pandalus borealis duplex-specific nuclease by Komagataella phaffii via promoter and peptide selection
Article Snippet: Duplex-specific nuclease (DSN) is a necessary component to digest double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrid duplexes specifically, and widely used in food analysis and biological sample treatment.. However, few cases were reported to produce DSN harnessing genetic engineering, which limited the application and pushed up the cost of DSN.. In this study, Pandalus borealis DSN gene was expressed by the famous microbial host, Komagataella phaffii, through three steps including promoter and signal peptide selection, DSN gene expression cassette increasing, cultivation optimization to reach 2.6 × 105 U/μL in fermentation broth.

other:

Article Title: Self-Assembled Nanoparticles with Kynureninase-Fc Fusion Protein and Pheophorbide A for Photodynamic Immunometabolic Cancer Therapy.
Article Snippet: The sequence of the gene was custom-synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

Clone Assay:

Article Title: Molecular chaperone effects on recombinant yield and binding characteristics of an ABA-specific scFv in Escherichia coli
Article Snippet: The E. coli strain BL21 (DE3) was purchased from Sangon Biotech (China). .. The ABA-scFv gene sequence, referenced from , was synthesized by Sangon Biotech and cloned into the pET30a vector to create the expression plasmid pET30a-ABA-scFv . .. To establish the chaperone co-expression systems, BL21 (DE3) competent cells were first transformed with one of five molecular chaperone plasmids: pG-KJE8 , pGro7 , pKJE7 , pG-Tf2 , or pTf16 (Takara, Japan), as detailed in .

Article Title: Development of a Highly Sensitive ELISA for Detecting Antibodies Against a Novel Variant Avian Reovirus Based on Dual σC and σB Antigens.
Article Snippet: .. Based on the nucleotide sequence of the LY383 strain (GenBank accession number: AWV55509), the σC and σB gene sequences were synthesized by Sangon Biotech (Shanghai, China) and subsequently cloned into the pMD19T vector (Takara, Kyoto, Japan). ..

Article Title: An Autocatalytic Molecular Sensor Enables Rapid, Sensitive, and One-Pot Detection of Nucleic Acids.
Article Snippet: Circulating nucleic acids are emerging disease markers whose clinical applications are hindered by the lack of rapid, sensitive, convenient, and cost-effective detection assays.. Inspired by the natural replication of virus, here, we developed a biomimetic one-step, one-pot, isothermal detection assay named RAPID to realize rapid and sensitive detection of nucleic acids with minimal reliance on instruments.. The core element of RAPID is an autocatalytic molecular sensor that exploits the viral replication endonuclease of duck circovirus (i.e., DCV) to transform rolling circle amplification (RCA) from linear to exponential.

Plasmid Preparation:

Article Title: Molecular chaperone effects on recombinant yield and binding characteristics of an ABA-specific scFv in Escherichia coli
Article Snippet: The E. coli strain BL21 (DE3) was purchased from Sangon Biotech (China). .. The ABA-scFv gene sequence, referenced from , was synthesized by Sangon Biotech and cloned into the pET30a vector to create the expression plasmid pET30a-ABA-scFv . .. To establish the chaperone co-expression systems, BL21 (DE3) competent cells were first transformed with one of five molecular chaperone plasmids: pG-KJE8 , pGro7 , pKJE7 , pG-Tf2 , or pTf16 (Takara, Japan), as detailed in .

Article Title: A genetically programmable, conjugation-free nanomedicine platform for modular antibody-guided drug delivery
Article Snippet: .. The scFv-ALB fusion gene sequence was synthesized by Sangon Biotech and subcloned into the mammalian expression vector pX17.4 to construct the recombinant plasmid pX17.4-scFv-ALB (αHER2). ..

Article Title: Production method of recombinant
Article Snippet: .. Strain and Plasmid Used in the Present Invention For genes of the 7β-HSDH and the GDH, Sangon Biotech (Shanghai) Co., Ltd. performs a codon optimization and a complete gene synthesis on a gene sequence according to codon bias of E. coli. .. An E. coli BL21(DE3) strain is purchased from Sangon Biotech (Shanghai) Co., Ltd.

Expressing:

Article Title: Molecular chaperone effects on recombinant yield and binding characteristics of an ABA-specific scFv in Escherichia coli
Article Snippet: The E. coli strain BL21 (DE3) was purchased from Sangon Biotech (China). .. The ABA-scFv gene sequence, referenced from , was synthesized by Sangon Biotech and cloned into the pET30a vector to create the expression plasmid pET30a-ABA-scFv . .. To establish the chaperone co-expression systems, BL21 (DE3) competent cells were first transformed with one of five molecular chaperone plasmids: pG-KJE8 , pGro7 , pKJE7 , pG-Tf2 , or pTf16 (Takara, Japan), as detailed in .

Article Title: A genetically programmable, conjugation-free nanomedicine platform for modular antibody-guided drug delivery
Article Snippet: .. The scFv-ALB fusion gene sequence was synthesized by Sangon Biotech and subcloned into the mammalian expression vector pX17.4 to construct the recombinant plasmid pX17.4-scFv-ALB (αHER2). ..

Construct:

Article Title: A genetically programmable, conjugation-free nanomedicine platform for modular antibody-guided drug delivery
Article Snippet: .. The scFv-ALB fusion gene sequence was synthesized by Sangon Biotech and subcloned into the mammalian expression vector pX17.4 to construct the recombinant plasmid pX17.4-scFv-ALB (αHER2). ..

Recombinant:

Article Title: A genetically programmable, conjugation-free nanomedicine platform for modular antibody-guided drug delivery
Article Snippet: .. The scFv-ALB fusion gene sequence was synthesized by Sangon Biotech and subcloned into the mammalian expression vector pX17.4 to construct the recombinant plasmid pX17.4-scFv-ALB (αHER2). ..

Derivative Assay:

Article Title: An Autocatalytic Molecular Sensor Enables Rapid, Sensitive, and One-Pot Detection of Nucleic Acids.
Article Snippet: Circulating nucleic acids are emerging disease markers whose clinical applications are hindered by the lack of rapid, sensitive, convenient, and cost-effective detection assays.. Inspired by the natural replication of virus, here, we developed a biomimetic one-step, one-pot, isothermal detection assay named RAPID to realize rapid and sensitive detection of nucleic acids with minimal reliance on instruments.. The core element of RAPID is an autocatalytic molecular sensor that exploits the viral replication endonuclease of duck circovirus (i.e., DCV) to transform rolling circle amplification (RCA) from linear to exponential.



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