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codon optimized peptide expression sequences  (Twist Bioscience)


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    Twist Bioscience codon optimized peptide expression sequences
    Codon Optimized Peptide Expression Sequences, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon/codon+experiments+expression+klhdc3+mouse+mouse+optimised+re/pm42264739-122-1-8
    Average 86 stars, based on 1 article reviews
    codon optimized peptide expression sequences - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Sequencing:

    Article Title: Engineering a Novel Bacterial Encapsulin for Programmable Surface Functionalization: From Single-Target to Mosaic Nanovaccines
    Article Snippet: The final preparation was adjusted to 1 mg/mL in 50 mM HEPES (pH 7.4), filtered through a 0.2 μm filter (Millipore), and stored at −30 °C for use in subsequent experiments. .. The E. coli codon optimised AmEnc-SpyCatcher (Am-S) sequence ( Table S2 ) was synthesised by Twist Bioscience into the pET-24(+) expression vector and transformed into E. coli BL21(DE3) competent cells (New England Biolabs). .. Cells from a single colony were cultured in a 5 mL starter LB broth culture containing 50 μg/mL of kanamycin (Thermo Fisher Scientific) and grown for 16–18 h at 37 °C with shaking at 200 rpm.

    Expressing:

    Article Title: Engineering a Novel Bacterial Encapsulin for Programmable Surface Functionalization: From Single-Target to Mosaic Nanovaccines
    Article Snippet: The final preparation was adjusted to 1 mg/mL in 50 mM HEPES (pH 7.4), filtered through a 0.2 μm filter (Millipore), and stored at −30 °C for use in subsequent experiments. .. The E. coli codon optimised AmEnc-SpyCatcher (Am-S) sequence ( Table S2 ) was synthesised by Twist Bioscience into the pET-24(+) expression vector and transformed into E. coli BL21(DE3) competent cells (New England Biolabs). .. Cells from a single colony were cultured in a 5 mL starter LB broth culture containing 50 μg/mL of kanamycin (Thermo Fisher Scientific) and grown for 16–18 h at 37 °C with shaking at 200 rpm.

    Article Title: Mechanistic characterization of insecticidal Shiva toxin interactions with Drosophila melanogaster nicotinic acetylcholine receptors.
    Article Snippet: Shiva family toxins are naturally occurring insecticidal peptides that target insect nicotinic acetylcholine receptors (nAChRs) and show strong utility as bioinsecticides.. However, the molecular basis of their interaction with insect nAChRs remains poorly understood.. In this study, we demonstrate that Shiva toxins exhibit no crossresistance with spinosad, indicating that they do not interact with the Drosophila nAChR α6 subunit.

    Transformation Assay:

    Article Title: Engineering a Novel Bacterial Encapsulin for Programmable Surface Functionalization: From Single-Target to Mosaic Nanovaccines
    Article Snippet: The final preparation was adjusted to 1 mg/mL in 50 mM HEPES (pH 7.4), filtered through a 0.2 μm filter (Millipore), and stored at −30 °C for use in subsequent experiments. .. The E. coli codon optimised AmEnc-SpyCatcher (Am-S) sequence ( Table S2 ) was synthesised by Twist Bioscience into the pET-24(+) expression vector and transformed into E. coli BL21(DE3) competent cells (New England Biolabs). .. Cells from a single colony were cultured in a 5 mL starter LB broth culture containing 50 μg/mL of kanamycin (Thermo Fisher Scientific) and grown for 16–18 h at 37 °C with shaking at 200 rpm.

    Clone Assay:

    Article Title: Biochemical and structural characterisation of MprF homologue, LpiA from Agrobacterium
    Article Snippet: .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience). .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience).

    Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication.
    Article Snippet: .. Human codon-optimised KLHDC3 was obtained from Twist Bioscience as a gene fragment with an N-terminal 3xFlag tag and cloned into the pAIP (pLenti SFFV IRES puromycin, Plasmid #74171, Addgene) and pLenti SFFV SV40 blasticidin vectors. .. 30,000–500,000 cells were acquired on a BD LSRII Fortessa and analysed with FlowJo software Version 9 or 10.0 (Treestar).

    Modification:

    Article Title: Biochemical and structural characterisation of MprF homologue, LpiA from Agrobacterium
    Article Snippet: .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience). .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience).

    Plasmid Preparation:

    Article Title: Biochemical and structural characterisation of MprF homologue, LpiA from Agrobacterium
    Article Snippet: .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience). .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience).

    Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication.
    Article Snippet: .. Human codon-optimised KLHDC3 was obtained from Twist Bioscience as a gene fragment with an N-terminal 3xFlag tag and cloned into the pAIP (pLenti SFFV IRES puromycin, Plasmid #74171, Addgene) and pLenti SFFV SV40 blasticidin vectors. .. 30,000–500,000 cells were acquired on a BD LSRII Fortessa and analysed with FlowJo software Version 9 or 10.0 (Treestar).

    Strep-tag:

    Article Title: Biochemical and structural characterisation of MprF homologue, LpiA from Agrobacterium
    Article Snippet: .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience). .. The codon optimised MprF gene of the A. fabrum ( formerly called A. tumefaciens strain C58, Uniprot ID - A9CHP8) was synthesised and cloned into a modified pET29b vector with a C-terminal strep-tag II and polyhistidine tag (Twist Bioscience).

    other:

    Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication.
    Article Snippet: From then on cells were counted and passaged every two to three days for 11 days, and cell pellets were collected for gDNA extraction and assessment of CRISPR-induced knockout frequency by TIDE (Brinkman et al, 2018) and ICE (accessed via Synthego) (Conant et al, 2022).

    Produced:

    Article Title: Actin disassembly triggers CNS myelin compaction and wrapping
    Article Snippet: All constructs were created using InFusion cloning (Takara Bio) by annealing one or more DNA fragments into the parent plasmid. .. DNA fragments were produced using PCR or directly synthesized as mouse codon-optimized Infusion-competent “gene fragments without adaptors” (Twist Biosciences), in both cases with the 15 base pair overhangs required for InFusion reactions. .. All constructs were sequence-verified by full plasmid sequencing (Plasmidsaurus) both at the time of cloning and after midiprepping (Qiagen) for AAV production.

    Polymerase Chain Reaction:

    Article Title: Actin disassembly triggers CNS myelin compaction and wrapping
    Article Snippet: All constructs were created using InFusion cloning (Takara Bio) by annealing one or more DNA fragments into the parent plasmid. .. DNA fragments were produced using PCR or directly synthesized as mouse codon-optimized Infusion-competent “gene fragments without adaptors” (Twist Biosciences), in both cases with the 15 base pair overhangs required for InFusion reactions. .. All constructs were sequence-verified by full plasmid sequencing (Plasmidsaurus) both at the time of cloning and after midiprepping (Qiagen) for AAV production.

    Synthesized:

    Article Title: Actin disassembly triggers CNS myelin compaction and wrapping
    Article Snippet: All constructs were created using InFusion cloning (Takara Bio) by annealing one or more DNA fragments into the parent plasmid. .. DNA fragments were produced using PCR or directly synthesized as mouse codon-optimized Infusion-competent “gene fragments without adaptors” (Twist Biosciences), in both cases with the 15 base pair overhangs required for InFusion reactions. .. All constructs were sequence-verified by full plasmid sequencing (Plasmidsaurus) both at the time of cloning and after midiprepping (Qiagen) for AAV production.

    Article Title: Mechanistic characterization of insecticidal Shiva toxin interactions with Drosophila melanogaster nicotinic acetylcholine receptors.
    Article Snippet: Shiva family toxins are naturally occurring insecticidal peptides that target insect nicotinic acetylcholine receptors (nAChRs) and show strong utility as bioinsecticides.. However, the molecular basis of their interaction with insect nAChRs remains poorly understood.. In this study, we demonstrate that Shiva toxins exhibit no crossresistance with spinosad, indicating that they do not interact with the Drosophila nAChR α6 subunit.



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