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codon optimized plasmid  (Addgene inc)


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    Addgene inc codon optimized plasmid
    Codon Optimized Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon/pCDNA3%2E1+(-)+%2B+codon+optimized+Brn3a+(Plasmid+%2386829)/pm41028822-60-1-6
    Average 93 stars, based on 18 article reviews
    codon optimized plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/ μl, NEB) to be phosphorylated.

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

    Clone Assay:

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/ μl, NEB) to be phosphorylated.

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

    Bacteria:

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/ μl, NEB) to be phosphorylated.

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

    DNA Extraction:

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/ μl, NEB) to be phosphorylated.

    Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
    Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

    Construct:

    Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
    Article Snippet: Stably-expressing 5-HT 2A/2B/2C receptor Flp-In 293 T-Rex Tetracycline inducible system (Invitrogen, mycoplasma-free) were used for calcium flux assays. .. Constructs used for these assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs, and then shuttled into pcDNA5/FRT/TO using Gibson Assembly. .. Cell lines were maintained in high-glucose DMEM (VWR) containing 10% FBS (Life Technologies), 10 μg/mL Blasticidin (GoldBio), and 100 μg/mL Hygromycin B (GoldBio).

    Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
    Article Snippet: All BRET assays were conducted using BRET2 in HEK293T cells (ATCC CRL-11268; mycoplasma-free), which were subcultured in high-glucose DMEM (VWR) supplemented with 10% FBS (Life Technologies). .. Constructs in G protein-dissociation BRET assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs. ..

    Article Title: Bioisosteric analogs of MDMA: Improving the pharmacological profile?
    Article Snippet: Stably- expressing 5- HT2A/2B/2C receptor Flp- In 293 T- Rex Tetracycline inducible system (Invitrogen, mycoplasma- free) were used for calcium flux assays (Lewis et al., 2023). .. 5- HT2A/2B/2C receptor constructs were derived from the codon- optimized Tango pcDNA3.1 library (Kroeze et al., 2015; Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de- Tango” constructs, and then shuttled into pcDNA5/ F I G U R E 1 3,4- Methylenedioxymethamphetamine (MDMA) and its analogs 1- (2,1,3- benzoxadiazol- 5- yl)- N- methylpropan- 2- amine (ODMA), 1- (2,1,3- benzothiadiazol- 5- yl)- N- methylpropan- 2- amine (TDMA), and 1- (2,1,3- benzoselenadiazol- 5- yl)- N- methylpropan- 2- amine (SeDMA) interact at low micromolar concentrations with the monoamine transporters. (a) Chemical structures of (±) MDMA and its analogs (±) ODMA, (±) TDMA, and (±) SeDMA, in which the methylenedioxy group or its chemical modification is highlighted in red, blue, purple, or green, respectively. (b) Uptake inhibition curves at human serotonin transporter (hSERT) (left panel), human dopamine transporter (hDAT) (middle panel), and human norepinephrine transporter (hNET) (right panel). ..

    Derivative Assay:

    Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
    Article Snippet: Stably-expressing 5-HT 2A/2B/2C receptor Flp-In 293 T-Rex Tetracycline inducible system (Invitrogen, mycoplasma-free) were used for calcium flux assays. .. Constructs used for these assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs, and then shuttled into pcDNA5/FRT/TO using Gibson Assembly. .. Cell lines were maintained in high-glucose DMEM (VWR) containing 10% FBS (Life Technologies), 10 μg/mL Blasticidin (GoldBio), and 100 μg/mL Hygromycin B (GoldBio).

    Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
    Article Snippet: All BRET assays were conducted using BRET2 in HEK293T cells (ATCC CRL-11268; mycoplasma-free), which were subcultured in high-glucose DMEM (VWR) supplemented with 10% FBS (Life Technologies). .. Constructs in G protein-dissociation BRET assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs. ..

    Article Title: Bioisosteric analogs of MDMA: Improving the pharmacological profile?
    Article Snippet: Stably- expressing 5- HT2A/2B/2C receptor Flp- In 293 T- Rex Tetracycline inducible system (Invitrogen, mycoplasma- free) were used for calcium flux assays (Lewis et al., 2023). .. 5- HT2A/2B/2C receptor constructs were derived from the codon- optimized Tango pcDNA3.1 library (Kroeze et al., 2015; Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de- Tango” constructs, and then shuttled into pcDNA5/ F I G U R E 1 3,4- Methylenedioxymethamphetamine (MDMA) and its analogs 1- (2,1,3- benzoxadiazol- 5- yl)- N- methylpropan- 2- amine (ODMA), 1- (2,1,3- benzothiadiazol- 5- yl)- N- methylpropan- 2- amine (TDMA), and 1- (2,1,3- benzoselenadiazol- 5- yl)- N- methylpropan- 2- amine (SeDMA) interact at low micromolar concentrations with the monoamine transporters. (a) Chemical structures of (±) MDMA and its analogs (±) ODMA, (±) TDMA, and (±) SeDMA, in which the methylenedioxy group or its chemical modification is highlighted in red, blue, purple, or green, respectively. (b) Uptake inhibition curves at human serotonin transporter (hSERT) (left panel), human dopamine transporter (hDAT) (middle panel), and human norepinephrine transporter (hNET) (right panel). ..

    Bioluminescence Resonance Energy Transfer:

    Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
    Article Snippet: All BRET assays were conducted using BRET2 in HEK293T cells (ATCC CRL-11268; mycoplasma-free), which were subcultured in high-glucose DMEM (VWR) supplemented with 10% FBS (Life Technologies). .. Constructs in G protein-dissociation BRET assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs. ..

    Expressing:

    Article Title: A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory target gene expression
    Article Snippet: .. The pcDNA3.1-FLAG -sarA , pcDNA3.1-FLAG -sarA:gp130 chimera, pcDNA3.1-FLAG- gp130dimer , pcDNA3.1-FLAG -gp130:sarA chimera codon-optimized for mammalian expression were previously described in and available from Addgene. .. Site-directed mutagenesis was carried out using Quikchange Lightning Site-Directed Mutagenesis Kit (Agilent, # 210518) (primers in Table S2).

    Modification:

    Article Title: Bioisosteric analogs of MDMA: Improving the pharmacological profile?
    Article Snippet: Stably- expressing 5- HT2A/2B/2C receptor Flp- In 293 T- Rex Tetracycline inducible system (Invitrogen, mycoplasma- free) were used for calcium flux assays (Lewis et al., 2023). .. 5- HT2A/2B/2C receptor constructs were derived from the codon- optimized Tango pcDNA3.1 library (Kroeze et al., 2015; Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de- Tango” constructs, and then shuttled into pcDNA5/ F I G U R E 1 3,4- Methylenedioxymethamphetamine (MDMA) and its analogs 1- (2,1,3- benzoxadiazol- 5- yl)- N- methylpropan- 2- amine (ODMA), 1- (2,1,3- benzothiadiazol- 5- yl)- N- methylpropan- 2- amine (TDMA), and 1- (2,1,3- benzoselenadiazol- 5- yl)- N- methylpropan- 2- amine (SeDMA) interact at low micromolar concentrations with the monoamine transporters. (a) Chemical structures of (±) MDMA and its analogs (±) ODMA, (±) TDMA, and (±) SeDMA, in which the methylenedioxy group or its chemical modification is highlighted in red, blue, purple, or green, respectively. (b) Uptake inhibition curves at human serotonin transporter (hSERT) (left panel), human dopamine transporter (hDAT) (middle panel), and human norepinephrine transporter (hNET) (right panel). ..

    Inhibition:

    Article Title: Bioisosteric analogs of MDMA: Improving the pharmacological profile?
    Article Snippet: Stably- expressing 5- HT2A/2B/2C receptor Flp- In 293 T- Rex Tetracycline inducible system (Invitrogen, mycoplasma- free) were used for calcium flux assays (Lewis et al., 2023). .. 5- HT2A/2B/2C receptor constructs were derived from the codon- optimized Tango pcDNA3.1 library (Kroeze et al., 2015; Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de- Tango” constructs, and then shuttled into pcDNA5/ F I G U R E 1 3,4- Methylenedioxymethamphetamine (MDMA) and its analogs 1- (2,1,3- benzoxadiazol- 5- yl)- N- methylpropan- 2- amine (ODMA), 1- (2,1,3- benzothiadiazol- 5- yl)- N- methylpropan- 2- amine (TDMA), and 1- (2,1,3- benzoselenadiazol- 5- yl)- N- methylpropan- 2- amine (SeDMA) interact at low micromolar concentrations with the monoamine transporters. (a) Chemical structures of (±) MDMA and its analogs (±) ODMA, (±) TDMA, and (±) SeDMA, in which the methylenedioxy group or its chemical modification is highlighted in red, blue, purple, or green, respectively. (b) Uptake inhibition curves at human serotonin transporter (hSERT) (left panel), human dopamine transporter (hDAT) (middle panel), and human norepinephrine transporter (hNET) (right panel). ..



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