coding region sequence cds (New England Biolabs)
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New England Biolabs
coding region sequence cds
Coding Region Sequence Cds, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coding+sequences+cds/Gibson+AssemblyMaster+Mix/pm41643674-823-1-15
Average 99 stars, based on 6183 article reviews
Coding Region Sequence Cds, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coding+sequences+cds/Gibson+AssemblyMaster+Mix/pm41643674-823-1-15
Average 99 stars, based on 6183 article reviews
coding region sequence cds - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the Purification:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Article Title: Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR Article Snippet: .. Briefly, DNA fragments were amplified from template plasmids or synthesized DNA fragments (listed in ) via the Q5 polymerase (New England BioLabs), purified with the Zymoclean gel DNA recovery kit (Zymo Research), and assembled with the Gel Extraction:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Clone Assay:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation Article Snippet: The two DNA halves were amplified separately using Phusion polymerase (NEB #M0530L) followed by an overlap extension polymerase chain reaction (PCR) with a forward primer containing a 5′ T7 promoter overhang. .. The amplified products were cloned into pcDNA3.1 using Plasmid Preparation:Article Title: The nuclear envelope protein TMEM209 is an integral component of the nuclear pore complex and interacts with Nup210 Article Snippet: .. For the Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the Construct:Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the Expressing:Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Sequencing:Article Title: Integrative Genomic, Single-Cell, and Functional Profiling of the CD48–CD244 Axis and NK-Cell Dysfunction in Multiple Myeloma Article Snippet: Gene fragments encoding murine CD48 (mature and immature isoforms; UniProt P18181), and dCas9-KRAB with a BFP2-mTag reporter were synthesized by Twist Bioscience (South San Francisco, CA). .. Constructs were assembled into lentiviral expression vectors using Amplification:Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation Article Snippet: The two DNA halves were amplified separately using Phusion polymerase (NEB #M0530L) followed by an overlap extension polymerase chain reaction (PCR) with a forward primer containing a 5′ T7 promoter overhang. .. The amplified products were cloned into pcDNA3.1 using Article Title: Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR Article Snippet: .. Briefly, DNA fragments were amplified from template plasmids or synthesized DNA fragments (listed in ) via the Q5 polymerase (New England BioLabs), purified with the Zymoclean gel DNA recovery kit (Zymo Research), and assembled with the Synthesized:Article Title: Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR Article Snippet: .. Briefly, DNA fragments were amplified from template plasmids or synthesized DNA fragments (listed in ) via the Q5 polymerase (New England BioLabs), purified with the Zymoclean gel DNA recovery kit (Zymo Research), and assembled with the Ligation:Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the |
